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Q9HAU5 (RENT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of nonsense transcripts 2
Alternative name(s):
Nonsense mRNA reducing factor 2
Up-frameshift suppressor 2 homolog
Short name=hUpf2
Gene names
Name:UPF2
Synonyms:KIAA1408, RENT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1272 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA. Ref.2 Ref.13 Ref.15

Subunit structure

Found in a post-splicing messenger ribonucleoprotein (mRNP) complex. Associates with the exon junction complex (EJC). Interacts with SMG1, EST1A, UPF1, UPF3A, UPF3B, EIF4A1 and EIF1. Ref.1 Ref.2 Ref.3 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14

Subcellular location

Cytoplasmperinuclear region Ref.1 Ref.2 Ref.3.

Tissue specificity

Ubiquitous. Ref.1

Sequence similarities

Contains 3 MIF4G domains.

Sequence caution

The sequence BAA92646.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9HAU5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9HAU5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     54-83: Missing.
     588-609: AMDFCMNMNTKANRKKLVRALF → EKAFCNGNLARVNLLLRMAVKK
     610-1272: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12721272Regulator of nonsense transcripts 2
PRO_0000097248

Regions

Domain168 – 431264MIF4G 1
Domain569 – 758190MIF4G 2
Domain773 – 986214MIF4G 3
Region94 – 13340Sufficient for interaction with UPF1
Region711 – 928218Sufficient for interaction with UPF3A and UPF3B
Region757 – 1272516Sufficient for interaction with EIF4A1 and EIF1
Region839 – 85921Binds to UPF3B
Region1084 – 1272189Sufficient for interaction with UPF1 C-terminus
Region1105 – 119894Necessary for interaction with UPF1
Region1105 – 112925Interaction with UPF1
Region1167 – 120741Interaction with UPF1
Coiled coil54 – 13481 Potential
Coiled coil487 – 55973 Potential
Compositional bias6 – 136131Glu/Lys-rich
Compositional bias1025 – 109470Glu-rich

Amino acid modifications

Modified residue10881Phosphothreonine Ref.17

Natural variations

Alternative sequence54 – 8330Missing in isoform 2.
VSP_012958
Alternative sequence588 – 60922AMDFC…VRALF → EKAFCNGNLARVNLLLRMAV KK in isoform 2.
VSP_012959
Alternative sequence610 – 1272663Missing in isoform 2.
VSP_012960
Natural variant4961N → S.
Corresponds to variant rs7079388 [ dbSNP | Ensembl ].
VAR_024345

Experimental info

Mutagenesis796 – 7972RK → EE: Strongly impairs RNA-binding.
Mutagenesis8471D → K: Does not abolish interaction with UPF3B. Ref.19
Mutagenesis851 – 8522ED → KR: Does not abolish interaction with UPF3B. Does not abolish interaction with UPF3B; when associated with D-854.
Mutagenesis8541R → D: Does not abolish interaction with UPF3B; when associated with K-851 and R-852. Ref.19
Mutagenesis8581E → R: Abolishes interaction with UPF3B and association with SMG1 and RBM8A; reduces phosphorylation of UPF1. Ref.14 Ref.19
Mutagenesis8941Y → A: Does not impair RNA-binding; when associated with A-932. Ref.19
Mutagenesis9321Y → A: Does not impair RNA-binding; when associated with A-894. Ref.19
Mutagenesis11131F → E: Abolishes interaction with UPF1.
Mutagenesis11201M → E: Decreases interaction with UPF1; does not reduce NMD efficiency. Ref.20
Mutagenesis11211M → E: Decreases interaction with UPF1; does not reduce NMD efficiency. Ref.20
Mutagenesis11231E → R: Decreases interaction with UPF1. Ref.20
Mutagenesis11691M → E: Decreases interaction with UPF1. Ref.20
Mutagenesis11711F → E: Abolishes interaction with UPF1; reduces NMD efficiency. Ref.20
Mutagenesis11711F → E: Greatly reduces NMD efficiency; when associated with E-1173 and E-1174. Ref.20
Mutagenesis11731M → E: Abolishes interaction with UPF1. Ref.20
Mutagenesis11731M → E: Greatly reduces NMD efficiency; when associated with E-1171 and E-1174. Ref.20
Mutagenesis11741L → E: Abolishes interaction with UPF1; reduces NMD efficiency. Ref.20
Mutagenesis11741L → E: Greatly reduces NMD efficiency; when associated with E-1171 and E-1173. Ref.20
Mutagenesis11761R → E: Decreases interaction with UPF1. Ref.20
Sequence conflict1191A → T in BAC04721. Ref.5
Sequence conflict3381F → L in BAC04721. Ref.5
Sequence conflict8441H → Q in AAG48509. Ref.2
Sequence conflict8441H → Q in BAA92646. Ref.4
Sequence conflict9691P → S in BAA92646. Ref.4

Secondary structure

........................................................................................................... 1272
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 95F3C57D2854BB44

FASTA1,272147,810
        10         20         30         40         50         60 
MPAERKKPAS MEEKDSLPNN KEKDCSERRT VSSKERPKDD IKLTAKKEVS KAPEDKKKRL 

        70         80         90        100        110        120 
EDDKRKKEDK ERKKKDEEKV KAEEESKKKE EEEKKKHQEE ERKKQEEQAK RQQEEEAAAQ 

       130        140        150        160        170        180 
MKEKEESIQL HQEAWERHHL RKELRSKNQN APDSRPEENF FSRLDSSLKK NTAFVKKLKT 

       190        200        210        220        230        240 
ITEQQRDSLS HDFNGLNLSK YIAEAVASIV EAKLKISDVN CAVHLCSLFH QRYADFAPSL 

       250        260        270        280        290        300 
LQVWKKHFEA RKEEKTPNIT KLRTDLRFIA ELTIVGIFTD KEGLSLIYEQ LKNIINADRE 

       310        320        330        340        350        360 
SHTHVSVVIS FCRHCGDDIA GLVPRKVKSA AEKFNLSFPP SEIISPEKQQ PFQNLLKEYF 

       370        380        390        400        410        420 
TSLTKHLKRD HRELQNTERQ NRRILHSKGE LSEDRHKQYE EFAMSYQKLL ANSQSLADLL 

       430        440        450        460        470        480 
DENMPDLPQD KPTPEEHGPG IDIFTPGKPG EYDLEGGIWE DEDARNFYEN LIDLKAFVPA 

       490        500        510        520        530        540 
ILFKDNEKSC QNKESNKDDT KEAKESKENK EVSSPDDLEL ELENLEINDD TLELEGGDEA 

       550        560        570        580        590        600 
EDLTKKLLDE QEQEDEEAST GSHLKLIVDA FLQQLPNCVN RDLIDKAAMD FCMNMNTKAN 

       610        620        630        640        650        660 
RKKLVRALFI VPRQRLDLLP FYARLVATLH PCMSDVAEDL CSMLRGDFRF HVRKKDQINI 

       670        680        690        700        710        720 
ETKNKTVRFI GELTKFKMFT KNDTLHCLKM LLSDFSHHHI EMACTLLETC GRFLFRSPES 

       730        740        750        760        770        780 
HLRTSVLLEQ MMRKKQAMHL DARYVTMVEN AYYYCNPPPA EKTVKKKRPP LQEYVRKLLY 

       790        800        810        820        830        840 
KDLSKVTTEK VLRQMRKLPW QDQEVKDYVI CCMINIWNVK YNSIHCVANL LAGLVLYQED 

       850        860        870        880        890        900 
VGIHVVDGVL EDIRLGMEVN QPKFNQRRIS SAKFLGELYN YRMVESAVIF RTLYSFTSFG 

       910        920        930        940        950        960 
VNPDGSPSSL DPPEHLFRIR LVCTILDTCG QYFDRGSSKR KLDCFLVYFQ RYVWWKKSLE 

       970        980        990       1000       1010       1020 
VWTKDHPFPI DIDYMISDTL ELLRPKIKLC NSLEESIRQV QDLEREFLIK LGLVNDKDSK 

      1030       1040       1050       1060       1070       1080 
DSMTEGENLE EDEEEEEGGA ETEEQSGNES EVNEPEEEEG SDNDDDEGEE EEEENTDYLT 

      1090       1100       1110       1120       1130       1140 
DSNKENETDE ENTEVMIKGG GLKHVPCVED EDFIQALDKM MLENLQQRSG ESVKVHQLDV 

      1150       1160       1170       1180       1190       1200 
AIPLHLKSQL RKGPPLGGGE GEAESADTMP FVMLTRKGNK QQFKILNVPM SSQLAANHWN 

      1210       1220       1230       1240       1250       1260 
QQQAEQEERM RMKKLTLDIN ERQEQEDYQE MLQSLAQRPA PANTNRERRP RYQHPKGAPN 

      1270 
ADLIFKTGGR RR 

« Hide

Isoform 2 [UniParc].

Checksum: FA8367F0C864C24E
Show »

FASTA57966,842

References

« Hide 'large scale' references
[1]"Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes."
Mendell J.T., Medghalchi S.M., Lake R.G., Noensie E.N., Dietz H.C.
Mol. Cell. Biol. 20:8944-8957(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH UPF1; EIF4A1 AND EIF1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Heart.
[2]"Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon."
Lykke-Andersen J., Shu M.-D., Steitz J.A.
Cell 103:1121-1131(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN NONSENSE-MEDIATED MRNA DECAY, INTERACTION WITH UPF1; UPF3A AND UPF3B, SUBCELLULAR LOCATION.
[3]"Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4)."
Serin G., Gersappe A., Black J.D., Aronoff R., Maquat L.E.
Mol. Cell. Biol. 21:209-223(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH UPF1; UPF3A AND UPF3B, SUBCELLULAR LOCATION.
Tissue: Cervix carcinoma.
[4]"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1023-1272.
[6]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 576-1272.
Tissue: Testis.
[10]"Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay."
Yamashita A., Ohnishi T., Kashima I., Taya Y., Ohno S.
Genes Dev. 15:2215-2228(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SMG1.
[11]"Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1."
Lykke-Andersen J., Shu M.-D., Steitz J.A.
Science 293:1836-1839(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A POST-SPLICING MRNP COMPLEX.
[12]"Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1."
Chiu S.-Y., Serin G., Ohara O., Maquat L.E.
RNA 9:77-87(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EST1A.
[13]"Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements."
Gehring N.H., Kunz J.B., Neu-Yilik G., Breit S., Viegas M.H., Hentze M.W., Kulozik A.E.
Mol. Cell 20:65-75(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN NONSENSE-MEDIATED MRNA DECAY, IDENTIFICATION IN A COMPLEX WITH UPF3B AND RNPS1.
[14]"Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay."
Kashima I., Yamashita A., Izumi N., Kataoka N., Morishita R., Hoshino S., Ohno M., Dreyfuss G., Ohno S.
Genes Dev. 20:355-367(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, MUTAGENESIS OF GLU-858.
[15]"NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity."
Chamieh H., Ballut L., Bonneau F., Le Hir H.
Nat. Struct. Mol. Biol. 15:85-93(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, RECONSTITUTION OF THE EJC CORE-UPF COMPLEX.
[16]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1088, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[19]"The structural basis for the interaction between nonsense-mediated mRNA decay factors UPF2 and UPF3."
Kadlec J., Izaurralde E., Cusack S.
Nat. Struct. Mol. Biol. 11:330-337(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 768-1015 IN COMPLEX WITH UPF3B RNP-LIKE DOMAIN, RNA-BINDING, MUTAGENESIS OF 796-ARG-ARG-797; ASP-847; 851-GLU-ASP-852; ARG-854; GLU-858; TYR-894 AND TYR-932.
[20]"Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2."
Clerici M., Mourao A., Gutsche I., Gehring N.H., Hentze M.W., Kulozik A., Kadlec J., Sattler M., Cusack S.
EMBO J. 28:2293-2306(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1105-1198 IN COMPLEX WITH UPF1, MUTAGENESIS OF MET-1120; MET-1121; GLU-1123; MET-1169; PHE-1171; MET-1173; LEU-1174 AND ARG-1176.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF301013 mRNA. Translation: AAG33225.1.
AY013249 mRNA. Translation: AAG48509.1.
AF318574 mRNA. Translation: AAG60689.1.
AB037829 mRNA. Translation: BAA92646.1. Different initiation.
AK000764 mRNA. Translation: BAA91369.1.
AK096191 mRNA. Translation: BAC04721.1.
AL138898, AC073160, AL645617 Genomic DNA. Translation: CAH73458.1.
AL138898, AC073160, AL645617 Genomic DNA. Translation: CAH73459.1.
AL645617, AC073160, AL138898 Genomic DNA. Translation: CAI16755.1.
AL645617, AC073160, AL138898 Genomic DNA. Translation: CAI16756.1.
CH471072 Genomic DNA. Translation: EAW86329.1.
CH471072 Genomic DNA. Translation: EAW86330.1.
CH471072 Genomic DNA. Translation: EAW86332.1.
CH471072 Genomic DNA. Translation: EAW86333.1.
BC114964 mRNA. Translation: AAI14965.1.
BC115737 mRNA. Translation: AAI15738.1.
AL080198 mRNA. Translation: CAB45771.1.
PIRT12507.
RefSeqNP_056357.1. NM_015542.3.
NP_542166.1. NM_080599.2.
UniGeneHs.370689.
Hs.610110.
Hs.732383.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UW4X-ray1.95B/D768-1015[»]
2WJVX-ray2.85D/E1105-1198[»]
4CEKX-ray2.35A455-757[»]
4CEMX-ray2.60A/B121-486[»]
ProteinModelPortalQ9HAU5.
SMRQ9HAU5. Positions 768-1014, 1105-1198.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117490. 86 interactions.
DIPDIP-31148N.
IntActQ9HAU5. 35 interactions.
MINTMINT-265195.

PTM databases

PhosphoSiteQ9HAU5.

Polymorphism databases

DMDM60390647.

Proteomic databases

PaxDbQ9HAU5.
PRIDEQ9HAU5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356352; ENSP00000348708; ENSG00000151461. [Q9HAU5-1]
ENST00000357604; ENSP00000350221; ENSG00000151461. [Q9HAU5-1]
ENST00000397053; ENSP00000380244; ENSG00000151461. [Q9HAU5-1]
GeneID26019.
KEGGhsa:26019.
UCSCuc001ila.3. human. [Q9HAU5-1]

Organism-specific databases

CTD26019.
GeneCardsGC10M011962.
HGNCHGNC:17854. UPF2.
MIM605529. gene.
neXtProtNX_Q9HAU5.
PharmGKBPA134945630.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG321770.
HOVERGENHBG079124.
InParanoidQ9HAU5.
KOK14327.
OMAAGIWEDE.
OrthoDBEOG70GMDS.
PhylomeDBQ9HAU5.
TreeFamTF300543.

Enzyme and pathway databases

ReactomeREACT_21257. Metabolism of RNA.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ9HAU5.
BgeeQ9HAU5.
GenevestigatorQ9HAU5.

Family and domain databases

Gene3D1.25.40.180. 4 hits.
InterProIPR016024. ARM-type_fold.
IPR016021. MIF4-like_typ_1/2/3.
IPR003890. MIF4G-like_typ-3.
IPR007193. Up-fram_suppressor-2.
[Graphical view]
PfamPF02854. MIF4G. 3 hits.
PF04050. Upf2. 1 hit.
[Graphical view]
SMARTSM00543. MIF4G. 3 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 3 hits.
ProtoNetSearch...

Other

ChiTaRSUPF2. human.
EvolutionaryTraceQ9HAU5.
GeneWikiUPF2.
GenomeRNAi26019.
NextBio47788.
PROQ9HAU5.
SOURCESearch...

Entry information

Entry nameRENT2_HUMAN
AccessionPrimary (citable) accession number: Q9HAU5
Secondary accession number(s): A6NLJ5 expand/collapse secondary AC list , D3DRS0, Q14BM1, Q5W0J4, Q8N8U1, Q9H1J2, Q9NWL1, Q9P2D9, Q9Y4M9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM