Reviewed, UniProtKB/Swiss-Prot Q9HAU0 (PKHA5_HUMAN)

Names and origin

Protein names

Recommended name:
    Pleckstrin homology domain-containing family A member 5
      Short name=PH domain-containing family A member 5
Alternative name(s):
    Phosphoinositol 3-phosphate-binding protein 2
      Short name=PEPP-2

Gene names

Name:	PLEKHA5
Synonyms:	KIAA1686, PEPP2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1116 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Tissue specificity	Highly expressed in heart and kidney. Ref.1
Sequence similarities	Contains 1 PH domain. Contains 2 WW domains.
Sequence caution	The sequence BAA91742.1 differs from that shown. Reason: Frameshift at position 759.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Molecular function	phosphatidylinositol binding Ref.1 Non-traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATN1	P54259	1	EBI-945934,EBI-945980
ATXN1	P54253	1	EBI-945934,EBI-930964
FOX1	Q9NWB1	1	EBI-945934,EBI-945906

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9HAU0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9HAU0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     615-615: T → TPEELTLLLIKLRRQQAELSSIREHTLAQLMQLKLEAHSPKNEILSHHLQRNTIYLDHQ

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9HAU0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     616-650: LSQDEGRGTLYKYRPEEVDIDAKLSRLCEQDKVVH → WGREKVATATGAAEAVASDTHLPRTGSSSPSLLCV
     651-1116: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9HAU0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     800-800: E → EEEEVVPPRPPLPRSYDFTEQPPIIPPLPSDSSSLLCYSRGPVHLPEEKKMYQVQGYPRNGSHC

Note: No experimental confirmation available.

Isoform 5 (identifier: Q9HAU0-5)

The sequence of this isoform differs from the canonical sequence as follows:
     785-840: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1116

1116

Pleckstrin homology domain-containing family A member 5

PRO_0000053883

Regions

Domain

10 – 43

34

WW 1

Domain

56 – 89

34

WW 2

Domain

169 – 268

100

PH

Amino acid modifications

Modified residue

128

1

Phosphotyrosine Ref.7

Modified residue

134

1

Phosphotyrosine Ref.7 Ref.14

Modified residue

353

1

Phosphotyrosine Ref.14

Modified residue

410

1

Phosphoserine Ref.13

Modified residue

438

1

Phosphothreonine Ref.11

Modified residue

476

1

Phosphothreonine Ref.11

Modified residue

568

1

Phosphoserine Ref.13 Ref.9

Modified residue

607

1

Phosphoserine Ref.13

Modified residue

855

1

Phosphoserine Ref.8 Ref.10 Ref.12

Modified residue

857

1

Phosphothreonine Ref.8

Modified residue

930

1

Phosphoserine Ref.13

Modified residue

933

1

Phosphoserine Ref.13 Ref.11 Ref.8 Ref.12 Ref.15

Modified residue

937

1

Phosphoserine Ref.13 Ref.11 Ref.8 Ref.12

Natural variations

Alternative sequence

615

1

T → TPEELTLLLIKLRRQQAELS SIREHTLAQLMQLKLEAHSP KNEILSHHLQRNTIYLDHQ in isoform 2.

VSP_009775

Alternative sequence

616 – 650

35

LSQDE…DKVVH → WGREKVATATGAAEAVASDT HLPRTGSSSPSLLCV in isoform 3.

VSP_009776

Alternative sequence

651 – 1116

466

Missing in isoform 3.

VSP_014595

Alternative sequence

785 – 840

56

Missing in isoform 5.

VSP_009777

Alternative sequence

800

1

E → EEEEVVPPRPPLPRSYDFTE QPPIIPPLPSDSSSLLCYSR GPVHLPEEKKMYQVQGYPRN GSHC in isoform 4.

VSP_009778

Experimental info

Sequence conflict

627

1

K → R in BAB14419. Ref.6

Sequence conflict

736

1

M → T in BAB14419. Ref.6

Sequence conflict

970

1

P → S in BAA91742. Ref.6

Secondary structure

1

.

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1116

Helix Strand Turn

Details...

Beta strand

172 – 179

8

Beta strand

182 – 184

3

Beta strand

187 – 195

9

Beta strand

198 – 204

7

Beta strand

209 – 214

6

Helix

216 – 218

3

Beta strand

219 – 223

5

Helix

226 – 228

3

Beta strand

234 – 239

6

Beta strand

241 – 243

3

Beta strand

246 – 249

4

Helix

253 – 267

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 327F792F644B9D48	FASTA	1,116	127,464
	10 20 30 40 50 60 MAADLNLEWI SLPRSWTYGI TRGGRVFFIN EEAKSTTWLH PVTGEAVVTG HRRQSTDLPT 70 80 90 100 110 120 GWEEAYTFEG ARYYINHNER KVTCKHPVTG QPSQDNCIFV VNEQTVATMT SEEKKERPIS 130 140 150 160 170 180 MINEASNYNV TSDYAVHPMS PVGRTSRASK KVHNFGKRSN SIKRNPNAPV VRRGWLYKQD 190 200 210 220 230 240 STGMKLWKKR WFVLSDLCLF YYRDEKEEGI LGSILLPSFQ IALLTSEDHI NRKYAFKAAH 250 260 270 280 290 300 PNMRTYYFCT DTGKEMELWM KAMLDAALVQ TEPVKRVDKI TSENAPTKET NNIPNHRVLI 310 320 330 340 350 360 KPEIQNNQKN KEMSKIEEKK ALEAEKYGFQ KDGQDRPLTK INSVKLNSLP SEYESGSACP 370 380 390 400 410 420 AQTVHYRPIN LSSSENKIVN VSLADLRGGN RPNTGPLYTE ADRVIQRTNS MQQLEQWIKI 430 440 450 460 470 480 QKGRGHEEET RGVISYQTLP RNMPSHRAQI MARYPEGYRT LPRNSKTRPE SICSVTPSTH 490 500 510 520 530 540 DKTLGPGAEE KRRSMRDDTM WQLYEWQQRQ FYNKQSTLPR HSTLSSPKTM VNISDQTMHS 550 560 570 580 590 600 IPTSPSHGSI AAYQGYSPQR TYRSEVSSPI QRGDVTIDRR HRAHHPKHVY VPDRRSVPAG 610 620 630 640 650 660 LTLQSVSPQS LQGKTLSQDE GRGTLYKYRP EEVDIDAKLS RLCEQDKVVH ALEEKLQQLH 670 680 690 700 710 720 KEKYTLEQAL LSASQEIEMH ADNPAAIQTV VLQRDDLQNG LLSTCRELSR ATAELERAWR 730 740 750 760 770 780 EYDKLEYDVT VTRNQMQEQL DHLGEVQTES AGIQRAQIQK ELWRIQDVME GLSKHKQQRG 790 800 810 820 830 840 TTEIGMIGSK PFSTVKYKNE GPDYRLYKSE PELTTVAEVD ESNGEEKSEP VSEIETSVVK 850 860 870 880 890 900 GSHFPVGVVP PRAKSPTPES STIASYVTLR KTKKMMDLRT ERPRSAVEQL CLAESTRPRM 910 920 930 940 950 960 TVEEQMERIR RHQQACLREK KKGLNVIGAS DQSPLQSPSN LRDNPFRTTQ TRRRDDKELD 970 980 990 1000 1010 1020 TAIRENDVKP DHETPATEIV QLKETEPQNV DFSKELKKTE NISYEMLFEP EPNGVNSVEM 1030 1040 1050 1060 1070 1080 MDKERNKDKM PEDVTFSPQD ETQTANHKPE EHPEENTKNS VDEQEETVIS YESTPEVSRG 1090 1100 1110 NQTMAVKSLS PSPESSASPV PSTQPQLTEG SHFMCV
	Isoform 2. Checksum: B82D29C6F281D311	FASTA	1,174	134,323
	10 20 30 40 50 60 MAADLNLEWI SLPRSWTYGI TRGGRVFFIN EEAKSTTWLH PVTGEAVVTG HRRQSTDLPT 70 80 90 100 110 120 GWEEAYTFEG ARYYINHNER KVTCKHPVTG QPSQDNCIFV VNEQTVATMT SEEKKERPIS 130 140 150 160 170 180 MINEASNYNV TSDYAVHPMS PVGRTSRASK KVHNFGKRSN SIKRNPNAPV VRRGWLYKQD 190 200 210 220 230 240 STGMKLWKKR WFVLSDLCLF YYRDEKEEGI LGSILLPSFQ IALLTSEDHI NRKYAFKAAH 250 260 270 280 290 300 PNMRTYYFCT DTGKEMELWM KAMLDAALVQ TEPVKRVDKI TSENAPTKET NNIPNHRVLI 310 320 330 340 350 360 KPEIQNNQKN KEMSKIEEKK ALEAEKYGFQ KDGQDRPLTK INSVKLNSLP SEYESGSACP 370 380 390 400 410 420 AQTVHYRPIN LSSSENKIVN VSLADLRGGN RPNTGPLYTE ADRVIQRTNS MQQLEQWIKI 430 440 450 460 470 480 QKGRGHEEET RGVISYQTLP RNMPSHRAQI MARYPEGYRT LPRNSKTRPE SICSVTPSTH 490 500 510 520 530 540 DKTLGPGAEE KRRSMRDDTM WQLYEWQQRQ FYNKQSTLPR HSTLSSPKTM VNISDQTMHS 550 560 570 580 590 600 IPTSPSHGSI AAYQGYSPQR TYRSEVSSPI QRGDVTIDRR HRAHHPKHVY VPDRRSVPAG 610 620 630 640 650 660 LTLQSVSPQS LQGKTPEELT LLLIKLRRQQ AELSSIREHT LAQLMQLKLE AHSPKNEILS 670 680 690 700 710 720 HHLQRNTIYL DHQLSQDEGR GTLYKYRPEE VDIDAKLSRL CEQDKVVHAL EEKLQQLHKE 730 740 750 760 770 780 KYTLEQALLS ASQEIEMHAD NPAAIQTVVL QRDDLQNGLL STCRELSRAT AELERAWREY 790 800 810 820 830 840 DKLEYDVTVT RNQMQEQLDH LGEVQTESAG IQRAQIQKEL WRIQDVMEGL SKHKQQRGTT 850 860 870 880 890 900 EIGMIGSKPF STVKYKNEGP DYRLYKSEPE LTTVAEVDES NGEEKSEPVS EIETSVVKGS 910 920 930 940 950 960 HFPVGVVPPR AKSPTPESST IASYVTLRKT KKMMDLRTER PRSAVEQLCL AESTRPRMTV 970 980 990 1000 1010 1020 EEQMERIRRH QQACLREKKK GLNVIGASDQ SPLQSPSNLR DNPFRTTQTR RRDDKELDTA 1030 1040 1050 1060 1070 1080 IRENDVKPDH ETPATEIVQL KETEPQNVDF SKELKKTENI SYEMLFEPEP NGVNSVEMMD 1090 1100 1110 1120 1130 1140 KERNKDKMPE DVTFSPQDET QTANHKPEEH PEENTKNSVD EQEETVISYE STPEVSRGNQ 1150 1160 1170 TMAVKSLSPS PESSASPVPS TQPQLTEGSH FMCV
	Isoform 3. Checksum: 98FCFC6BD6243232	FASTA	650	73,909
	10 20 30 40 50 60 MAADLNLEWI SLPRSWTYGI TRGGRVFFIN EEAKSTTWLH PVTGEAVVTG HRRQSTDLPT 70 80 90 100 110 120 GWEEAYTFEG ARYYINHNER KVTCKHPVTG QPSQDNCIFV VNEQTVATMT SEEKKERPIS 130 140 150 160 170 180 MINEASNYNV TSDYAVHPMS PVGRTSRASK KVHNFGKRSN SIKRNPNAPV VRRGWLYKQD 190 200 210 220 230 240 STGMKLWKKR WFVLSDLCLF YYRDEKEEGI LGSILLPSFQ IALLTSEDHI NRKYAFKAAH 250 260 270 280 290 300 PNMRTYYFCT DTGKEMELWM KAMLDAALVQ TEPVKRVDKI TSENAPTKET NNIPNHRVLI 310 320 330 340 350 360 KPEIQNNQKN KEMSKIEEKK ALEAEKYGFQ KDGQDRPLTK INSVKLNSLP SEYESGSACP 370 380 390 400 410 420 AQTVHYRPIN LSSSENKIVN VSLADLRGGN RPNTGPLYTE ADRVIQRTNS MQQLEQWIKI 430 440 450 460 470 480 QKGRGHEEET RGVISYQTLP RNMPSHRAQI MARYPEGYRT LPRNSKTRPE SICSVTPSTH 490 500 510 520 530 540 DKTLGPGAEE KRRSMRDDTM WQLYEWQQRQ FYNKQSTLPR HSTLSSPKTM VNISDQTMHS 550 560 570 580 590 600 IPTSPSHGSI AAYQGYSPQR TYRSEVSSPI QRGDVTIDRR HRAHHPKHVY VPDRRSVPAG 610 620 630 640 650 LTLQSVSPQS LQGKTWGREK VATATGAAEA VASDTHLPRT GSSSPSLLCV
	Isoform 4. Checksum: A7E7827DDD2166DC	FASTA	1,179	134,593
	10 20 30 40 50 60 MAADLNLEWI SLPRSWTYGI TRGGRVFFIN EEAKSTTWLH PVTGEAVVTG HRRQSTDLPT 70 80 90 100 110 120 GWEEAYTFEG ARYYINHNER KVTCKHPVTG QPSQDNCIFV VNEQTVATMT SEEKKERPIS 130 140 150 160 170 180 MINEASNYNV TSDYAVHPMS PVGRTSRASK KVHNFGKRSN SIKRNPNAPV VRRGWLYKQD 190 200 210 220 230 240 STGMKLWKKR WFVLSDLCLF YYRDEKEEGI LGSILLPSFQ IALLTSEDHI NRKYAFKAAH 250 260 270 280 290 300 PNMRTYYFCT DTGKEMELWM KAMLDAALVQ TEPVKRVDKI TSENAPTKET NNIPNHRVLI 310 320 330 340 350 360 KPEIQNNQKN KEMSKIEEKK ALEAEKYGFQ KDGQDRPLTK INSVKLNSLP SEYESGSACP 370 380 390 400 410 420 AQTVHYRPIN LSSSENKIVN VSLADLRGGN RPNTGPLYTE ADRVIQRTNS MQQLEQWIKI 430 440 450 460 470 480 QKGRGHEEET RGVISYQTLP RNMPSHRAQI MARYPEGYRT LPRNSKTRPE SICSVTPSTH 490 500 510 520 530 540 DKTLGPGAEE KRRSMRDDTM WQLYEWQQRQ FYNKQSTLPR HSTLSSPKTM VNISDQTMHS 550 560 570 580 590 600 IPTSPSHGSI AAYQGYSPQR TYRSEVSSPI QRGDVTIDRR HRAHHPKHVY VPDRRSVPAG 610 620 630 640 650 660 LTLQSVSPQS LQGKTLSQDE GRGTLYKYRP EEVDIDAKLS RLCEQDKVVH ALEEKLQQLH 670 680 690 700 710 720 KEKYTLEQAL LSASQEIEMH ADNPAAIQTV VLQRDDLQNG LLSTCRELSR ATAELERAWR 730 740 750 760 770 780 EYDKLEYDVT VTRNQMQEQL DHLGEVQTES AGIQRAQIQK ELWRIQDVME GLSKHKQQRG 790 800 810 820 830 840 TTEIGMIGSK PFSTVKYKNE EEEVVPPRPP LPRSYDFTEQ PPIIPPLPSD SSSLLCYSRG 850 860 870 880 890 900 PVHLPEEKKM YQVQGYPRNG SHCGPDYRLY KSEPELTTVA EVDESNGEEK SEPVSEIETS 910 920 930 940 950 960 VVKGSHFPVG VVPPRAKSPT PESSTIASYV TLRKTKKMMD LRTERPRSAV EQLCLAESTR 970 980 990 1000 1010 1020 PRMTVEEQME RIRRHQQACL REKKKGLNVI GASDQSPLQS PSNLRDNPFR TTQTRRRDDK 1030 1040 1050 1060 1070 1080 ELDTAIREND VKPDHETPAT EIVQLKETEP QNVDFSKELK KTENISYEML FEPEPNGVNS 1090 1100 1110 1120 1130 1140 VEMMDKERNK DKMPEDVTFS PQDETQTANH KPEEHPEENT KNSVDEQEET VISYESTPEV 1150 1160 1170 SRGNQTMAVK SLSPSPESSA SPVPSTQPQL TEGSHFMCV
	Isoform 5. Checksum: EC6B2DC66DC64F6A	FASTA	1,060	121,272
	10 20 30 40 50 60 MAADLNLEWI SLPRSWTYGI TRGGRVFFIN EEAKSTTWLH PVTGEAVVTG HRRQSTDLPT 70 80 90 100 110 120 GWEEAYTFEG ARYYINHNER KVTCKHPVTG QPSQDNCIFV VNEQTVATMT SEEKKERPIS 130 140 150 160 170 180 MINEASNYNV TSDYAVHPMS PVGRTSRASK KVHNFGKRSN SIKRNPNAPV VRRGWLYKQD 190 200 210 220 230 240 STGMKLWKKR WFVLSDLCLF YYRDEKEEGI LGSILLPSFQ IALLTSEDHI NRKYAFKAAH 250 260 270 280 290 300 PNMRTYYFCT DTGKEMELWM KAMLDAALVQ TEPVKRVDKI TSENAPTKET NNIPNHRVLI 310 320 330 340 350 360 KPEIQNNQKN KEMSKIEEKK ALEAEKYGFQ KDGQDRPLTK INSVKLNSLP SEYESGSACP 370 380 390 400 410 420 AQTVHYRPIN LSSSENKIVN VSLADLRGGN RPNTGPLYTE ADRVIQRTNS MQQLEQWIKI 430 440 450 460 470 480 QKGRGHEEET RGVISYQTLP RNMPSHRAQI MARYPEGYRT LPRNSKTRPE SICSVTPSTH 490 500 510 520 530 540 DKTLGPGAEE KRRSMRDDTM WQLYEWQQRQ FYNKQSTLPR HSTLSSPKTM VNISDQTMHS 550 560 570 580 590 600 IPTSPSHGSI AAYQGYSPQR TYRSEVSSPI QRGDVTIDRR HRAHHPKHVY VPDRRSVPAG 610 620 630 640 650 660 LTLQSVSPQS LQGKTLSQDE GRGTLYKYRP EEVDIDAKLS RLCEQDKVVH ALEEKLQQLH 670 680 690 700 710 720 KEKYTLEQAL LSASQEIEMH ADNPAAIQTV VLQRDDLQNG LLSTCRELSR ATAELERAWR 730 740 750 760 770 780 EYDKLEYDVT VTRNQMQEQL DHLGEVQTES AGIQRAQIQK ELWRIQDVME GLSKHKQQRG 790 800 810 820 830 840 TTEIGSHFPV GVVPPRAKSP TPESSTIASY VTLRKTKKMM DLRTERPRSA VEQLCLAEST 850 860 870 880 890 900 RPRMTVEEQM ERIRRHQQAC LREKKKGLNV IGASDQSPLQ SPSNLRDNPF RTTQTRRRDD 910 920 930 940 950 960 KELDTAIREN DVKPDHETPA TEIVQLKETE PQNVDFSKEL KKTENISYEM LFEPEPNGVN 970 980 990 1000 1010 1020 SVEMMDKERN KDKMPEDVTF SPQDETQTAN HKPEEHPEEN TKNSVDEQEE TVISYESTPE 1030 1040 1050 1060 VSRGNQTMAV KSLSPSPESS ASPVPSTQPQ LTEGSHFMCV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities." Dowler S.J., Currie R.A., Campbell D.G., Deak M., Kular G., Downes C.P., Alessi D.R. Biochem. J. 351:19-31(2000) [PubMed: 11001876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O. DNA Res. 7:347-355(2000) [PubMed: 11214970] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Amygdala.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 159-1116 (ISOFORM 3). Tissue: Placenta.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 625-1116 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 664-1116 (ISOFORM 5). Tissue: Teratocarcinoma.
[7]	"Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." Zhang Y., Wolf-Yadlin A., Ross P.L., Pappin D.J., Rush J., Lauffenburger D.A., White F.M. Mol. Cell. Proteomics 4:1240-1250(2005) [PubMed: 15951569] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-128 AND TYR-134, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-855; THR-857; SER-933 AND SER-937, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-568, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction." Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R. Mol. Cell. Proteomics 6:1952-1967(2007) [PubMed: 17693683] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-855, MASS SPECTROMETRY.
[11]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-438; THR-476; SER-933 AND SER-937, MASS SPECTROMETRY.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-855; SER-933 AND SER-937, MASS SPECTROMETRY.
[13]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-410; SER-568; SER-607; SER-930; SER-933 AND SER-937, MASS SPECTROMETRY.
[14]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-134 AND TYR-353, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[15]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-933, MASS SPECTROMETRY. Tissue: T-cell.
[16]	"Solution structure of the PH domain of pleckstrin homology domain-containing protein family A member 5 from human." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 156-271.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF302150 mRNA. Translation: AAG22817.1.
AB051473 mRNA. Translation: BAB21777.2. Different initiation.
AL834259 mRNA. Translation: CAD38934.1.
BC000969 mRNA. Translation: AAH00969.1.
BC013133 mRNA. Translation: AAH13133.3.
BC127092 mRNA. Translation: AAI27093.1.
AK001529 mRNA. Translation: BAA91742.1. Frameshift.
AK023127 mRNA. Translation: BAB14419.1. Different initiation.

IPI

IPI00029515.
IPI00169401.
IPI00409581.
IPI00409582.
IPI00409583.

RefSeq

NP_001137293.1.
NP_061885.2.

UniGene

Hs.188614

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DKP	NMR	-	A	157-271	[»]

SMR

Q9HAU0. Positions 2-87.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9HAU0. 3 interactions.

PTM databases

PhosphoSite

Q9HAU0.

Proteomic databases

PRIDE

Q9HAU0.

Genome annotation databases

Ensembl

ENST00000299275; ENSP00000299275; ENSG00000052126; Homo sapiens. [Genome view]

GeneID

54477.

KEGG

hsa:54477.

UCSC

uc001rea.1. human.
uc001reb.1. human.

Organism-specific databases

CTD

54477.

GeneCards

GC12P019174.

H-InvDB

HIX0010473.

HGNC

HGNC:30036. PLEKHA5.

MIM

607770. gene.

PharmGKB

PA134949896.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q9HAU0.

Gene expression databases

ArrayExpress

Q9HAU0.

Bgee

Q9HAU0.

CleanEx

HS_PLEKHA5.

Genevestigator

Q9HAU0.

GermOnline

ENSG00000052126. Homo sapiens.

Family and domain databases

InterPro

IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR001202. WW_Rsp5_WWP.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.

Pfam

PF00169. PH. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00456. WW. 2 hits.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

56792.

SOURCE

Search...

Entry information

Entry name

PKHA5_HUMAN

Accession

Primary (citable) accession number: Q9HAU0
Secondary accession number(s): A0JP03 , Q8N3K6, Q96DY9, Q9BVR4, Q9C0H7, Q9H924, Q9NVK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.