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Q9HAP6

- LIN7B_HUMAN

UniProt

Q9HAP6 - LIN7B_HUMAN

Protein

Protein lin-7 homolog B

Gene

LIN7B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ASIC3 acid-evoked currents by stabilizing the channel at the cell surface By similarity.By similarity

    GO - Molecular functioni

    1. protein domain specific binding Source: BHF-UCL

    GO - Biological processi

    1. exocytosis Source: UniProtKB-KW
    2. neurotransmitter secretion Source: Ensembl
    3. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein lin-7 homolog B
    Short name:
    Lin-7B
    Short name:
    hLin7B
    Alternative name(s):
    Mammalian lin-seven protein 2
    Short name:
    MALS-2
    Vertebrate lin-7 homolog 2
    Short name:
    Veli-2
    Short name:
    hVeli2
    Gene namesi
    Name:LIN7B
    Synonyms:MALS2, VELI2
    ORF Names:UNQ3116/PRO10200
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:17788. LIN7B.

    Subcellular locationi

    Cell membrane; Peripheral membrane protein. Basolateral cell membrane; Peripheral membrane protein. Cell junction By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity; Peripheral membrane protein By similarity. Cell junctiontight junction By similarity. Cell junctionsynapsesynaptosome By similarity
    Note: Enriched in synaptosomes and at epithelial cell-cell junctions By similarity. Mainly basolateral in renal epithelial cells.By similarity

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB-SubCell
    2. neuron projection Source: UniProtKB-SubCell
    3. postsynaptic density Source: UniProtKB-SubCell
    4. postsynaptic membrane Source: UniProtKB-KW
    5. tight junction Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome, Tight junction

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134914453.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 207207Protein lin-7 homolog BPRO_0000189626Add
    BLAST

    Proteomic databases

    PaxDbiQ9HAP6.
    PRIDEiQ9HAP6.

    PTM databases

    PhosphoSiteiQ9HAP6.

    Expressioni

    Gene expression databases

    BgeeiQ9HAP6.
    CleanExiHS_LIN7B.
    GenevestigatoriQ9HAP6.

    Organism-specific databases

    HPAiHPA046419.

    Interactioni

    Subunit structurei

    Forms two exclusive ternary complexes with CASK and APBA1 or CASKIN1. Can also interact with other modular proteins containing protein-protein interaction domains like MPP5, MPP6, MPP7, DLG1, DLG2 and DLG3 through its L27 domain. Interacts with DLG4 and GRIN2B as well as CDH1 and CTNNB1, the channels KCNJ12/Kir2.2, KCNJ4/Kir2.3 and probably KCNJ2/Kir2.1 and SLC6A12/BGT-1 via its PDZ domain. The association of LIN7A with cadherin and beta-catenin is calcium-dependent, occurs at synaptic junctions and requires the actin cytoskeleton. Interacts with EGFR, ERBB2, ERBB3 and ERBB4 with both PDZ and KID domains. Associates with KIF17 via APBA1 By similarity. Interacts with ASIC3. Interacts with TOPK. Interacts with RTKN.By similarity4 Publications

    Protein-protein interaction databases

    BioGridi122079. 7 interactions.
    IntActiQ9HAP6. 1 interaction.
    MINTiMINT-224720.
    STRINGi9606.ENSP00000221459.

    Structurei

    Secondary structure

    1
    207
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi93 – 975
    Beta strandi105 – 1084
    Beta strandi110 – 1145
    Beta strandi118 – 1225
    Helixi127 – 1315
    Beta strandi139 – 1435
    Helixi153 – 16210
    Beta strandi165 – 1717

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DKRNMR-A93-172[»]
    ProteinModelPortaliQ9HAP6.
    SMRiQ9HAP6. Positions 3-65, 93-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9HAP6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 6556L27PROSITE-ProRule annotationAdd
    BLAST
    Domaini93 – 17583PDZPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1 – 1313Kinase interacting siteBy similarityAdd
    BLAST

    Domaini

    The kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane.By similarity
    The PDZ domain regulates endocytosis and recycling of the receptor at the membrane.By similarity
    The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes.By similarity

    Sequence similaritiesi

    Belongs to the lin-7 family.Curated
    Contains 1 L27 domain.PROSITE-ProRule annotation
    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG320117.
    HOGENOMiHOG000285929.
    HOVERGENiHBG052329.
    InParanoidiQ9HAP6.
    OMAiCLERDVC.
    OrthoDBiEOG75MVXG.
    PhylomeDBiQ9HAP6.
    TreeFamiTF316850.

    Family and domain databases

    Gene3Di2.30.42.10. 1 hit.
    InterProiIPR004172. L27.
    IPR014775. L27_C.
    IPR017365. Lin-7_homologue.
    IPR001478. PDZ.
    [Graphical view]
    PfamiPF02828. L27. 1 hit.
    PF00595. PDZ. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038039. Lin-7_homologue. 1 hit.
    SMARTiSM00569. L27. 1 hit.
    SM00228. PDZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 1 hit.
    PROSITEiPS51022. L27. 1 hit.
    PS50106. PDZ. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9HAP6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAALVEPLGL ERDVSRAVEL LERLQRSGEL PPQKLQALQR VLQSRFCSAI    50
    REVYEQLYDT LDITGSAEIR AHATAKATVA AFTASEGHAH PRVVELPKTD 100
    EGLGFNIMGG KEQNSPIYIS RVIPGGVADR HGGLKRGDQL LSVNGVSVEG 150
    EQHEKAVELL KAAQGSVKLV VRYTPRVLEE MEARFEKMRS ARRRQQHQSY 200
    SSLESRG 207
    Length:207
    Mass (Da):22,896
    Last modified:March 1, 2001 - v1
    Checksum:i63189D82706B9B00
    GO
    Isoform 2 (identifier: Q9HAP6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         77-146: Missing.

    Show »
    Length:137
    Mass (Da):15,620
    Checksum:iE24A181A837542DD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651G → R in AI826082. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei77 – 14670Missing in isoform 2. 1 PublicationVSP_042156Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF311862 mRNA. Translation: AAG34117.1.
    AY358744 mRNA. Translation: AAQ89104.1.
    AI826082 mRNA. No translation available.
    BC027618 mRNA. Translation: AAH27618.1.
    CCDSiCCDS12757.1. [Q9HAP6-1]
    RefSeqiNP_071448.1. NM_022165.2. [Q9HAP6-1]
    XP_005259206.1. XM_005259149.1. [Q9HAP6-2]
    UniGeneiHs.221737.

    Genome annotation databases

    EnsembliENST00000221459; ENSP00000221459; ENSG00000104863. [Q9HAP6-1]
    ENST00000391864; ENSP00000375737; ENSG00000104863. [Q9HAP6-2]
    GeneIDi64130.
    KEGGihsa:64130.
    UCSCiuc002pmp.3. human. [Q9HAP6-1]

    Polymorphism databases

    DMDMi59798472.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF311862 mRNA. Translation: AAG34117.1 .
    AY358744 mRNA. Translation: AAQ89104.1 .
    AI826082 mRNA. No translation available.
    BC027618 mRNA. Translation: AAH27618.1 .
    CCDSi CCDS12757.1. [Q9HAP6-1 ]
    RefSeqi NP_071448.1. NM_022165.2. [Q9HAP6-1 ]
    XP_005259206.1. XM_005259149.1. [Q9HAP6-2 ]
    UniGenei Hs.221737.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DKR NMR - A 93-172 [» ]
    ProteinModelPortali Q9HAP6.
    SMRi Q9HAP6. Positions 3-65, 93-175.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122079. 7 interactions.
    IntActi Q9HAP6. 1 interaction.
    MINTi MINT-224720.
    STRINGi 9606.ENSP00000221459.

    PTM databases

    PhosphoSitei Q9HAP6.

    Polymorphism databases

    DMDMi 59798472.

    Proteomic databases

    PaxDbi Q9HAP6.
    PRIDEi Q9HAP6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000221459 ; ENSP00000221459 ; ENSG00000104863 . [Q9HAP6-1 ]
    ENST00000391864 ; ENSP00000375737 ; ENSG00000104863 . [Q9HAP6-2 ]
    GeneIDi 64130.
    KEGGi hsa:64130.
    UCSCi uc002pmp.3. human. [Q9HAP6-1 ]

    Organism-specific databases

    CTDi 64130.
    GeneCardsi GC19P049623.
    HGNCi HGNC:17788. LIN7B.
    HPAi HPA046419.
    MIMi 612331. gene.
    neXtProti NX_Q9HAP6.
    PharmGKBi PA134914453.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG320117.
    HOGENOMi HOG000285929.
    HOVERGENi HBG052329.
    InParanoidi Q9HAP6.
    OMAi CLERDVC.
    OrthoDBi EOG75MVXG.
    PhylomeDBi Q9HAP6.
    TreeFami TF316850.

    Miscellaneous databases

    EvolutionaryTracei Q9HAP6.
    GeneWikii LIN7B.
    GenomeRNAii 64130.
    NextBioi 66020.
    PROi Q9HAP6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9HAP6.
    CleanExi HS_LIN7B.
    Genevestigatori Q9HAP6.

    Family and domain databases

    Gene3Di 2.30.42.10. 1 hit.
    InterProi IPR004172. L27.
    IPR014775. L27_C.
    IPR017365. Lin-7_homologue.
    IPR001478. PDZ.
    [Graphical view ]
    Pfami PF02828. L27. 1 hit.
    PF00595. PDZ. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038039. Lin-7_homologue. 1 hit.
    SMARTi SM00569. L27. 1 hit.
    SM00228. PDZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 1 hit.
    PROSITEi PS51022. L27. 1 hit.
    PS50106. PDZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Basolateral membrane expression of the Kir 2.3 channel is coordinated by PDZ interaction with Lin-7/CASK complex."
      Olsen O., Liu H., Wade J.B., Merot J., Welling P.A.
      Am. J. Physiol. 282:C183-C195(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH KCNJ4 AND CASK (ISOFORM 1).
      Tissue: Kidney.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain and Testis.
    4. "Polar expression of ErbB-2/HER2 in epithelia. Bimodal regulation by Lin-7."
      Shelly M., Mosesson Y., Citri A., Lavi S., Zwang Y., Melamed-Book N., Aroeti B., Yarden Y.
      Dev. Cell 5:475-486(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH EGFR.
    5. "PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current."
      Hruska-Hageman A.M., Benson C.J., Leonard A.S., Price M.P., Welsh M.J.
      J. Biol. Chem. 279:46962-46968(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ASIC3.
    6. "Identification of a cell polarity-related protein, Lin-7B, as a binding partner for a Rho effector, Rhotekin, and their possible interaction in neurons."
      Sudo K., Ito H., Iwamoto I., Morishita R., Asano T., Nagata K.
      Neurosci. Res. 56:347-355(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RTKN.
    7. "The stardust family protein MPP7 forms a tripartite complex with LIN7 and DLG1 that regulates the stability and localization of DLG1 to cell junctions."
      Bohl J., Brimer N., Lyons C., Vande Pol S.B.
      J. Biol. Chem. 282:9392-9400(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MPP7.
    8. "Solution structure of the PDZ domain from human LIN-7 homolog B."
      RIKEN structural genomics initiative (RSGI)
      Submitted (OCT-2006) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 93-172.

    Entry informationi

    Entry nameiLIN7B_HUMAN
    AccessioniPrimary (citable) accession number: Q9HAP6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3