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Q9HAP6 (LIN7B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein lin-7 homolog B

Short name=Lin-7B
Short name=hLin7B
Alternative name(s):
Mammalian lin-seven protein 2
Short name=MALS-2
Vertebrate lin-7 homolog 2
Short name=Veli-2
Short name=hVeli2
Gene names
Name:LIN7B
Synonyms:MALS2, VELI2
ORF Names:UNQ3116/PRO10200
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ASIC3 acid-evoked currents by stabilizing the channel at the cell surface By similarity. Ref.1

Subunit structure

Forms two exclusive ternary complexes with CASK and APBA1 or CASKIN1. Can also interact with other modular proteins containing protein-protein interaction domains like MPP5, MPP6, MPP7, DLG1, DLG2 and DLG3 through its L27 domain. Interacts with DLG4 and GRIN2B as well as CDH1 and CTNNB1, the channels KCNJ12/Kir2.2, KCNJ4/Kir2.3 and probably KCNJ2/Kir2.1 and SLC6A12/BGT-1 via its PDZ domain. The association of LIN7A with cadherin and beta-catenin is calcium-dependent, occurs at synaptic junctions and requires the actin cytoskeleton. Interacts with EGFR, ERBB2, ERBB3 and ERBB4 with both PDZ and KID domains. Associates with KIF17 via APBA1 By similarity. Interacts with ASIC3. Interacts with TOPK. Interacts with RTKN. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7

Subcellular location

Cell membrane; Peripheral membrane protein. Basolateral cell membrane; Peripheral membrane protein. Cell junction By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density; Peripheral membrane protein By similarity. Cell junctiontight junction By similarity. Cell junctionsynapsesynaptosome By similarity. Note: Enriched in synaptosomes and at epithelial cell-cell junctions By similarity. Mainly basolateral in renal epithelial cells. Ref.1 Ref.4

Domain

The kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane By similarity.

The PDZ domain regulates endocytosis and recycling of the receptor at the membrane By similarity.

The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes By similarity.

Sequence similarities

Belongs to the lin-7 family.

Contains 1 L27 domain.

Contains 1 PDZ (DHR) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9HAP6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9HAP6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     77-146: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 207207Protein lin-7 homolog B
PRO_0000189626

Regions

Domain10 – 6556L27
Domain93 – 17583PDZ
Motif1 – 1313Kinase interacting site By similarity

Natural variations

Alternative sequence77 – 14670Missing in isoform 2.
VSP_042156

Experimental info

Sequence conflict651G → R in AI826082. Ref.3

Secondary structure

................. 207
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 63189D82706B9B00

FASTA20722,896
        10         20         30         40         50         60 
MAALVEPLGL ERDVSRAVEL LERLQRSGEL PPQKLQALQR VLQSRFCSAI REVYEQLYDT 

        70         80         90        100        110        120 
LDITGSAEIR AHATAKATVA AFTASEGHAH PRVVELPKTD EGLGFNIMGG KEQNSPIYIS 

       130        140        150        160        170        180 
RVIPGGVADR HGGLKRGDQL LSVNGVSVEG EQHEKAVELL KAAQGSVKLV VRYTPRVLEE 

       190        200 
MEARFEKMRS ARRRQQHQSY SSLESRG 

« Hide

Isoform 2 [UniParc].

Checksum: E24A181A837542DD
Show »

FASTA13715,620

References

« Hide 'large scale' references
[1]"Basolateral membrane expression of the Kir 2.3 channel is coordinated by PDZ interaction with Lin-7/CASK complex."
Olsen O., Liu H., Wade J.B., Merot J., Welling P.A.
Am. J. Physiol. 282:C183-C195(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH KCNJ4 AND CASK (ISOFORM 1).
Tissue: Kidney.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Testis.
[4]"Polar expression of ErbB-2/HER2 in epithelia. Bimodal regulation by Lin-7."
Shelly M., Mosesson Y., Citri A., Lavi S., Zwang Y., Melamed-Book N., Aroeti B., Yarden Y.
Dev. Cell 5:475-486(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH EGFR.
[5]"PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current."
Hruska-Hageman A.M., Benson C.J., Leonard A.S., Price M.P., Welsh M.J.
J. Biol. Chem. 279:46962-46968(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ASIC3.
[6]"Identification of a cell polarity-related protein, Lin-7B, as a binding partner for a Rho effector, Rhotekin, and their possible interaction in neurons."
Sudo K., Ito H., Iwamoto I., Morishita R., Asano T., Nagata K.
Neurosci. Res. 56:347-355(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RTKN.
[7]"The stardust family protein MPP7 forms a tripartite complex with LIN7 and DLG1 that regulates the stability and localization of DLG1 to cell junctions."
Bohl J., Brimer N., Lyons C., Vande Pol S.B.
J. Biol. Chem. 282:9392-9400(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MPP7.
[8]"Solution structure of the PDZ domain from human LIN-7 homolog B."
RIKEN structural genomics initiative (RSGI)
Submitted (OCT-2006) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 93-172.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF311862 mRNA. Translation: AAG34117.1.
AY358744 mRNA. Translation: AAQ89104.1.
AI826082 mRNA. No translation available.
BC027618 mRNA. Translation: AAH27618.1.
CCDSCCDS12757.1. [Q9HAP6-1]
RefSeqNP_071448.1. NM_022165.2. [Q9HAP6-1]
XP_005259206.1. XM_005259149.1. [Q9HAP6-2]
UniGeneHs.221737.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DKRNMR-A93-172[»]
ProteinModelPortalQ9HAP6.
SMRQ9HAP6. Positions 3-65, 93-175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122079. 7 interactions.
IntActQ9HAP6. 1 interaction.
MINTMINT-224720.
STRING9606.ENSP00000221459.

PTM databases

PhosphoSiteQ9HAP6.

Polymorphism databases

DMDM59798472.

Proteomic databases

PaxDbQ9HAP6.
PRIDEQ9HAP6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000221459; ENSP00000221459; ENSG00000104863. [Q9HAP6-1]
ENST00000391864; ENSP00000375737; ENSG00000104863. [Q9HAP6-2]
GeneID64130.
KEGGhsa:64130.
UCSCuc002pmp.3. human. [Q9HAP6-1]

Organism-specific databases

CTD64130.
GeneCardsGC19P049623.
HGNCHGNC:17788. LIN7B.
HPAHPA046419.
MIM612331. gene.
neXtProtNX_Q9HAP6.
PharmGKBPA134914453.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG320117.
HOGENOMHOG000285929.
HOVERGENHBG052329.
InParanoidQ9HAP6.
OMACLERDVC.
OrthoDBEOG75MVXG.
PhylomeDBQ9HAP6.
TreeFamTF316850.

Gene expression databases

BgeeQ9HAP6.
CleanExHS_LIN7B.
GenevestigatorQ9HAP6.

Family and domain databases

Gene3D2.30.42.10. 1 hit.
InterProIPR004172. L27.
IPR014775. L27_C.
IPR017365. Lin-7_homologue.
IPR001478. PDZ.
[Graphical view]
PfamPF02828. L27. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
PIRSFPIRSF038039. Lin-7_homologue. 1 hit.
SMARTSM00569. L27. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS51022. L27. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9HAP6.
GeneWikiLIN7B.
GenomeRNAi64130.
NextBio66020.
PROQ9HAP6.
SOURCESearch...

Entry information

Entry nameLIN7B_HUMAN
AccessionPrimary (citable) accession number: Q9HAP6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM