SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9HAP6

- LIN7B_HUMAN

UniProt

Q9HAP6 - LIN7B_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Protein lin-7 homolog B
Gene
LIN7B, MALS2, VELI2, UNQ3116/PRO10200
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ASIC3 acid-evoked currents by stabilizing the channel at the cell surface By similarity.1 Publication

GO - Molecular functioni

  1. protein domain specific binding Source: BHF-UCL

GO - Biological processi

  1. exocytosis Source: UniProtKB-KW
  2. neurotransmitter secretion Source: Ensembl
  3. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein lin-7 homolog B
Short name:
Lin-7B
Short name:
hLin7B
Alternative name(s):
Mammalian lin-seven protein 2
Short name:
MALS-2
Vertebrate lin-7 homolog 2
Short name:
Veli-2
Short name:
hVeli2
Gene namesi
Name:LIN7B
Synonyms:MALS2, VELI2
ORF Names:UNQ3116/PRO10200
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:17788. LIN7B.

Subcellular locationi

Cell membrane; Peripheral membrane protein. Basolateral cell membrane; Peripheral membrane protein. Cell junction By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density; Peripheral membrane protein By similarity. Cell junctiontight junction By similarity. Cell junctionsynapsesynaptosome By similarity
Note: Enriched in synaptosomes and at epithelial cell-cell junctions By similarity. Mainly basolateral in renal epithelial cells.2 Publications

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB-SubCell
  2. neuron projection Source: UniProtKB-SubCell
  3. postsynaptic density Source: UniProtKB-SubCell
  4. postsynaptic membrane Source: UniProtKB-KW
  5. tight junction Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome, Tight junction

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134914453.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Protein lin-7 homolog B
PRO_0000189626Add
BLAST

Proteomic databases

PaxDbiQ9HAP6.
PRIDEiQ9HAP6.

PTM databases

PhosphoSiteiQ9HAP6.

Expressioni

Gene expression databases

BgeeiQ9HAP6.
CleanExiHS_LIN7B.
GenevestigatoriQ9HAP6.

Organism-specific databases

HPAiHPA046419.

Interactioni

Subunit structurei

Forms two exclusive ternary complexes with CASK and APBA1 or CASKIN1. Can also interact with other modular proteins containing protein-protein interaction domains like MPP5, MPP6, MPP7, DLG1, DLG2 and DLG3 through its L27 domain. Interacts with DLG4 and GRIN2B as well as CDH1 and CTNNB1, the channels KCNJ12/Kir2.2, KCNJ4/Kir2.3 and probably KCNJ2/Kir2.1 and SLC6A12/BGT-1 via its PDZ domain. The association of LIN7A with cadherin and beta-catenin is calcium-dependent, occurs at synaptic junctions and requires the actin cytoskeleton. Interacts with EGFR, ERBB2, ERBB3 and ERBB4 with both PDZ and KID domains. Associates with KIF17 via APBA1 By similarity. Interacts with ASIC3. Interacts with TOPK. Interacts with RTKN.5 Publications

Protein-protein interaction databases

BioGridi122079. 7 interactions.
IntActiQ9HAP6. 1 interaction.
MINTiMINT-224720.
STRINGi9606.ENSP00000221459.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi93 – 975
Beta strandi105 – 1084
Beta strandi110 – 1145
Beta strandi118 – 1225
Helixi127 – 1315
Beta strandi139 – 1435
Helixi153 – 16210
Beta strandi165 – 1717

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DKRNMR-A93-172[»]
ProteinModelPortaliQ9HAP6.
SMRiQ9HAP6. Positions 3-65, 93-175.

Miscellaneous databases

EvolutionaryTraceiQ9HAP6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 6556L27
Add
BLAST
Domaini93 – 17583PDZ
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 1313Kinase interacting site By similarity
Add
BLAST

Domaini

The kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane By similarity.
The PDZ domain regulates endocytosis and recycling of the receptor at the membrane By similarity.
The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes By similarity.

Sequence similaritiesi

Belongs to the lin-7 family.
Contains 1 L27 domain.
Contains 1 PDZ (DHR) domain.

Phylogenomic databases

eggNOGiNOG320117.
HOGENOMiHOG000285929.
HOVERGENiHBG052329.
InParanoidiQ9HAP6.
OMAiCLERDVC.
OrthoDBiEOG75MVXG.
PhylomeDBiQ9HAP6.
TreeFamiTF316850.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR004172. L27.
IPR014775. L27_C.
IPR017365. Lin-7_homologue.
IPR001478. PDZ.
[Graphical view]
PfamiPF02828. L27. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
PIRSFiPIRSF038039. Lin-7_homologue. 1 hit.
SMARTiSM00569. L27. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51022. L27. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9HAP6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAALVEPLGL ERDVSRAVEL LERLQRSGEL PPQKLQALQR VLQSRFCSAI    50
REVYEQLYDT LDITGSAEIR AHATAKATVA AFTASEGHAH PRVVELPKTD 100
EGLGFNIMGG KEQNSPIYIS RVIPGGVADR HGGLKRGDQL LSVNGVSVEG 150
EQHEKAVELL KAAQGSVKLV VRYTPRVLEE MEARFEKMRS ARRRQQHQSY 200
SSLESRG 207
Length:207
Mass (Da):22,896
Last modified:March 1, 2001 - v1
Checksum:i63189D82706B9B00
GO
Isoform 2 (identifier: Q9HAP6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-146: Missing.

Show »
Length:137
Mass (Da):15,620
Checksum:iE24A181A837542DD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei77 – 14670Missing in isoform 2.
VSP_042156Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651G → R in AI826082. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF311862 mRNA. Translation: AAG34117.1.
AY358744 mRNA. Translation: AAQ89104.1.
AI826082 mRNA. No translation available.
BC027618 mRNA. Translation: AAH27618.1.
CCDSiCCDS12757.1. [Q9HAP6-1]
RefSeqiNP_071448.1. NM_022165.2. [Q9HAP6-1]
XP_005259206.1. XM_005259149.1. [Q9HAP6-2]
UniGeneiHs.221737.

Genome annotation databases

EnsembliENST00000221459; ENSP00000221459; ENSG00000104863. [Q9HAP6-1]
ENST00000391864; ENSP00000375737; ENSG00000104863. [Q9HAP6-2]
GeneIDi64130.
KEGGihsa:64130.
UCSCiuc002pmp.3. human. [Q9HAP6-1]

Polymorphism databases

DMDMi59798472.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF311862 mRNA. Translation: AAG34117.1 .
AY358744 mRNA. Translation: AAQ89104.1 .
AI826082 mRNA. No translation available.
BC027618 mRNA. Translation: AAH27618.1 .
CCDSi CCDS12757.1. [Q9HAP6-1 ]
RefSeqi NP_071448.1. NM_022165.2. [Q9HAP6-1 ]
XP_005259206.1. XM_005259149.1. [Q9HAP6-2 ]
UniGenei Hs.221737.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DKR NMR - A 93-172 [» ]
ProteinModelPortali Q9HAP6.
SMRi Q9HAP6. Positions 3-65, 93-175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122079. 7 interactions.
IntActi Q9HAP6. 1 interaction.
MINTi MINT-224720.
STRINGi 9606.ENSP00000221459.

PTM databases

PhosphoSitei Q9HAP6.

Polymorphism databases

DMDMi 59798472.

Proteomic databases

PaxDbi Q9HAP6.
PRIDEi Q9HAP6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000221459 ; ENSP00000221459 ; ENSG00000104863 . [Q9HAP6-1 ]
ENST00000391864 ; ENSP00000375737 ; ENSG00000104863 . [Q9HAP6-2 ]
GeneIDi 64130.
KEGGi hsa:64130.
UCSCi uc002pmp.3. human. [Q9HAP6-1 ]

Organism-specific databases

CTDi 64130.
GeneCardsi GC19P049623.
HGNCi HGNC:17788. LIN7B.
HPAi HPA046419.
MIMi 612331. gene.
neXtProti NX_Q9HAP6.
PharmGKBi PA134914453.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG320117.
HOGENOMi HOG000285929.
HOVERGENi HBG052329.
InParanoidi Q9HAP6.
OMAi CLERDVC.
OrthoDBi EOG75MVXG.
PhylomeDBi Q9HAP6.
TreeFami TF316850.

Miscellaneous databases

EvolutionaryTracei Q9HAP6.
GeneWikii LIN7B.
GenomeRNAii 64130.
NextBioi 66020.
PROi Q9HAP6.
SOURCEi Search...

Gene expression databases

Bgeei Q9HAP6.
CleanExi HS_LIN7B.
Genevestigatori Q9HAP6.

Family and domain databases

Gene3Di 2.30.42.10. 1 hit.
InterProi IPR004172. L27.
IPR014775. L27_C.
IPR017365. Lin-7_homologue.
IPR001478. PDZ.
[Graphical view ]
Pfami PF02828. L27. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view ]
PIRSFi PIRSF038039. Lin-7_homologue. 1 hit.
SMARTi SM00569. L27. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 1 hit.
PROSITEi PS51022. L27. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Basolateral membrane expression of the Kir 2.3 channel is coordinated by PDZ interaction with Lin-7/CASK complex."
    Olsen O., Liu H., Wade J.B., Merot J., Welling P.A.
    Am. J. Physiol. 282:C183-C195(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH KCNJ4 AND CASK (ISOFORM 1).
    Tissue: Kidney.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Testis.
  4. "Polar expression of ErbB-2/HER2 in epithelia. Bimodal regulation by Lin-7."
    Shelly M., Mosesson Y., Citri A., Lavi S., Zwang Y., Melamed-Book N., Aroeti B., Yarden Y.
    Dev. Cell 5:475-486(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH EGFR.
  5. "PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current."
    Hruska-Hageman A.M., Benson C.J., Leonard A.S., Price M.P., Welsh M.J.
    J. Biol. Chem. 279:46962-46968(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ASIC3.
  6. "Identification of a cell polarity-related protein, Lin-7B, as a binding partner for a Rho effector, Rhotekin, and their possible interaction in neurons."
    Sudo K., Ito H., Iwamoto I., Morishita R., Asano T., Nagata K.
    Neurosci. Res. 56:347-355(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RTKN.
  7. "The stardust family protein MPP7 forms a tripartite complex with LIN7 and DLG1 that regulates the stability and localization of DLG1 to cell junctions."
    Bohl J., Brimer N., Lyons C., Vande Pol S.B.
    J. Biol. Chem. 282:9392-9400(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPP7.
  8. "Solution structure of the PDZ domain from human LIN-7 homolog B."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 93-172.

Entry informationi

Entry nameiLIN7B_HUMAN
AccessioniPrimary (citable) accession number: Q9HAP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi