Q9HAK2 (COE2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor COE2

Alternative name(s):
Early B-cell factor 2
Short name=EBF-2

Gene names

Name:	EBF2
Synonyms:	COE2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' By similarity.
Subunit structure	Forms either a homodimer or a heterodimer with a related family member By similarity.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the COE family. Contains 1 IPT/TIG domain.
Sequence caution	The sequence BAB13843.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Transcription factor COE2		PRO_0000107828
Regions
	Domain	253 – 336	84	IPT/TIG
	Zinc finger	150 – 169	20	C5-type Potential
	Region	62 – 65	4	Interaction with DNA By similarity
	Region	196 – 203	8	Interaction with DNA By similarity
	Region	235 – 238	4	Interaction with DNA By similarity
	Compositional bias	453 – 534	82	Pro/Ser/Thr-rich
Sites
	Site	162	1	Interaction with DNA By similarity
	Site	171	1	Interaction with DNA By similarity
Natural variations
	Natural variant	559	1	G → S. Corresponds to variant rs17054477 [ dbSNP | Ensembl ].		VAR_048754
Experimental info
	Sequence conflict	513	1	S → L in BAB13843. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9HAK2 [UniParc]. Last modified December 12, 2006. Version 4. Checksum: 05FCDBC9CB81EC89	FASTA	575	62,650
	10 20 30 40 50 60 MFGIQDTLGR GPTLKEKSLG AEMDSVRSWV RNVGVVDANV AAQSGVALSR AHFEKQPPSN 70 80 90 100 110 120 LRKSNFFHFV LALYDRQGQP VEIERTAFVD FVENDKEQGN EKTNNGTHYK LQLLYSNGVR 130 140 150 160 170 180 TEQDLYVRLI DSVTKQPIAY EGQNKNPEMC RVLLTHEVMC SRCCEKKSCG NRNETPSDPV 190 200 210 220 230 240 IIDRFFLKFF LKCNQNCLKT AGNPRDMRRF QVVLSTTVNV DGHVLAVSDN MFVHNNSKHG 250 260 270 280 290 300 RRARRLDPSE ATPCIKAISP SEGWTTGGAM VIIIGDNFFD GLQVVFGTML VWSELITPHA 310 320 330 340 350 360 IRVQTPPRHI PGVVEVTLSY KSKQFCKGAP GRFIYTALNE PTIDYGFQRL QKVIPRHPGD 370 380 390 400 410 420 PERLAKEMLL KRAADLVEAL YGTPHNNQDI ILKRAADIAE ALYSVPRNPS QLPALSSSPA 430 440 450 460 470 480 HSGMMGINSY GSQLGVSISE STQGNNQGYI RNTSSISPRG YSSSSTPQQS NYSTSSNSMN 490 500 510 520 530 540 GYSNVPMANL GVPGSPGFLN GSPTGSPYGI MSSSPTVGSS STSSILPFSS SVFPAVKQKS 550 560 570 AFAPVIRPQG SPSPACSSGN GNGFRAMTGL VVPPM

References

[1]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed: 16421571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 233-575. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 240-575. Tissue: Brain and Fetal brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC023566 Genomic DNA. No translation available. AC090103 Genomic DNA. No translation available. AK021562 mRNA. Translation: BAB13843.1. Different initiation. BC069665 mRNA. Translation: AAH69665.1. BC069726 mRNA. Translation: AAH69726.1. BC069747 mRNA. Translation: AAH69747.1. BC069768 mRNA. Translation: AAH69768.1. BC074794 mRNA. Translation: AAH74794.2. BC113478 mRNA. Translation: AAI13479.1. BC113504 mRNA. Translation: AAI13505.1.
IPI	IPI00165598.
RefSeq	NP_073150.2. NM_022659.3.
UniGene	Hs.710674.
3D structure databases
ProteinModelPortal	Q9HAK2.
SMR	Q9HAK2. Positions 35-384.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9HAK2.
Polymorphism databases
DMDM	119370337.
Proteomic databases
PRIDE	Q9HAK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000408929; ENSP00000386178; ENSG00000221818.
GeneID	64641.
KEGG	hsa:64641.
UCSC	uc003xes.1. human.
Organism-specific databases
CTD	64641.
GeneCards	GC08M025757.
HGNC	HGNC:19090. EBF2.
HPA	CAB037026.
MIM	609934. gene.
neXtProt	NX_Q9HAK2.
PharmGKB	PA38794.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000014051.
HOGENOM	HBG444229.
HOVERGEN	HBG005108.
InParanoid	Q9HAK2.
OMA	TMEYATP.
OrthoDB	EOG4TXBRK.
PhylomeDB	Q9HAK2.
Gene expression databases
ArrayExpress	Q9HAK2.
Bgee	Q9HAK2.
CleanEx	HS_EBF2.
Genevestigator	Q9HAK2.
GermOnline	ENSG00000134025. Homo sapiens.
Family and domain databases
InterPro	IPR011598. HLH_DNA-bd. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR002909. IPT_TIG_rcpt. IPR003523. Transcription_factor_COE. IPR018350. Transcription_factor_COE_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K09103.
PANTHER	PTHR10747. TF_COE. 1 hit.
Pfam	PF01833. TIG. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. SM00429. IPT. 1 hit. [Graphical view]
SUPFAM	SSF81296. Ig_E-set. 1 hit.
PROSITE	PS01345. COE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	66577.
SOURCE	Search...

Entry information

Entry name

COE2_HUMAN

Accession

Primary (citable) accession number: Q9HAK2
Secondary accession number(s): A0PJM4 , A6NMF7, Q6DK36, Q6IS86, Q6ISA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 86 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.