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Q9HA77

- SYCM_HUMAN

UniProt

Q9HA77 - SYCM_HUMAN

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Protein
Probable cysteine--tRNA ligase, mitochondrial
Gene
CARS2, OK/SW-cl.10
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi78 – 781Zinc By similarity
Metal bindingi257 – 2571Zinc By similarity
Metal bindingi282 – 2821Zinc By similarity
Metal bindingi286 – 2861Zinc By similarity
Binding sitei320 – 3201ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-tRNA ligase activity Source: RefGenome
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cysteinyl-tRNA aminoacylation Source: RefGenome
  2. gene expression Source: Reactome
  3. tRNA aminoacylation for protein translation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_15302. Mitochondrial tRNA aminoacylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cysteine--tRNA ligase, mitochondrial (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:CARS2
ORF Names:OK/SW-cl.10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 13

Organism-specific databases

HGNCiHGNC:25695. CARS2.

Subcellular locationi

Mitochondrion matrix By similarity UniRule annotation

GO - Cellular componenti

  1. cytosol Source: RefGenome
  2. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162381083.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 564Probable cysteine--tRNA ligase, mitochondrialUniRule annotationPRO_0000250741
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Proteomic databases

MaxQBiQ9HA77.
PaxDbiQ9HA77.
PeptideAtlasiQ9HA77.
PRIDEiQ9HA77.

PTM databases

PhosphoSiteiQ9HA77.

Expressioni

Gene expression databases

ArrayExpressiQ9HA77.
BgeeiQ9HA77.
CleanExiHS_CARS2.
GenevestigatoriQ9HA77.

Organism-specific databases

HPAiHPA041776.
HPA043935.

Interactioni

Protein-protein interaction databases

BioGridi122730. 1 interaction.
IntActiQ9HA77. 1 interaction.
STRINGi9606.ENSP00000257347.

Structurei

3D structure databases

ProteinModelPortaliQ9HA77.
SMRiQ9HA77. Positions 52-417.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi80 – 9011"HIGH" regionUniRule annotation
Add
BLAST
Motifi317 – 3215"KMSKS" regionUniRule annotation

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0215.
HOGENOMiHOG000245250.
HOVERGENiHBG094012.
InParanoidiQ9HA77.
KOiK01883.
OMAiDKRHASD.
OrthoDBiEOG7XPZ5N.
PhylomeDBiQ9HA77.
TreeFamiTF300384.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HA77-1 [UniParc]FASTAAdd to Basket

« Hide

MLRTTRGPGL GPPLLQAALG LGRAGWHWPA GRAASGGRGR AWLQPTGRET    50
GVQVYNSLTG RKEPLIVAHA EAASWYSCGP TVYDHAHLGH ACSYVRFDII 100
RRILTKVFGC SIVMVMGITD VDDKIIKRAN EMNISPASLA SLYEEDFKQD 150
MAALKVLPPT VYLRVTENIP QIISFIEGII ARGNAYSTAK GNVYFDLKSR 200
GDKYGKLVGV VPGPVGEPAD SDKRHASDFA LWKAAKPQEV FWASPWGPGR 250
PGWHIECSAI ASMVFGSQLD IHSGGIDLAF PHHENEIAQC EVFHQCEQWG 300
NYFLHSGHLH AKGKEEKMSK SLKNYITIKD FLKTFSPDVF RFFCLRSSYR 350
SAIDYSDSAM LQAQQLLLGL GSFLEDARAY MKGQLACGSV REAMLWERLS 400
STKRAVKAAL ADDFDTPRVV DAILGLAHHG NGQLRASLKE PEGPRSPAVF 450
GAIISYFEQF FETVGISLAN QQYVSGDGSE ATLHGVVDEL VRFRQKVRQF 500
ALAMPEATGD ARRQQLLERQ PLLEACDTLR RGLTAHGINI KDRSSTTSTW 550
ELLDQRTKDQ KSAG 564
Length:564
Mass (Da):62,224
Last modified:March 1, 2001 - v1
Checksum:i92F8B615E6657D50
GO

Sequence cautioni

The sequence BAB93499.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti440 – 4401E → K.
Corresponds to variant rs965189 [ dbSNP | Ensembl ].
VAR_034523
Natural varianti555 – 5551Q → P.1 Publication
Corresponds to variant rs1043886 [ dbSNP | Ensembl ].
VAR_034524

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 20221RGNAY…KSRGD → SWERLFNGKRQCLLRSESLE ET in BAB93499. 1 Publication
Add
BLAST
Sequence conflicti207 – 22317LVGVV…ADSDK → IGRRGPWSSPETSGLLTS in BAB93499. 1 Publication
Add
BLAST
Sequence conflicti227 – 2271S → N in BAB93499. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK022180 mRNA. Translation: BAB13978.1.
AL157820, AL139385 Genomic DNA. Translation: CAI16971.1.
AL139385, AL157820 Genomic DNA. Translation: CAI17004.1.
BC007220 mRNA. Translation: AAH07220.1.
AB062436 mRNA. Translation: BAB93499.1. Different initiation.
CCDSiCCDS9514.1.
RefSeqiNP_078813.1. NM_024537.2.
UniGeneiHs.508725.

Genome annotation databases

EnsembliENST00000257347; ENSP00000257347; ENSG00000134905.
GeneIDi79587.
KEGGihsa:79587.
UCSCiuc001vrd.2. human.

Polymorphism databases

DMDMi74761587.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK022180 mRNA. Translation: BAB13978.1 .
AL157820 , AL139385 Genomic DNA. Translation: CAI16971.1 .
AL139385 , AL157820 Genomic DNA. Translation: CAI17004.1 .
BC007220 mRNA. Translation: AAH07220.1 .
AB062436 mRNA. Translation: BAB93499.1 . Different initiation.
CCDSi CCDS9514.1.
RefSeqi NP_078813.1. NM_024537.2.
UniGenei Hs.508725.

3D structure databases

ProteinModelPortali Q9HA77.
SMRi Q9HA77. Positions 52-417.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122730. 1 interaction.
IntActi Q9HA77. 1 interaction.
STRINGi 9606.ENSP00000257347.

Chemistry

DrugBanki DB00151. L-Cysteine.

PTM databases

PhosphoSitei Q9HA77.

Polymorphism databases

DMDMi 74761587.

Proteomic databases

MaxQBi Q9HA77.
PaxDbi Q9HA77.
PeptideAtlasi Q9HA77.
PRIDEi Q9HA77.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000257347 ; ENSP00000257347 ; ENSG00000134905 .
GeneIDi 79587.
KEGGi hsa:79587.
UCSCi uc001vrd.2. human.

Organism-specific databases

CTDi 79587.
GeneCardsi GC13M111293.
HGNCi HGNC:25695. CARS2.
HPAi HPA041776.
HPA043935.
MIMi 612800. gene.
neXtProti NX_Q9HA77.
PharmGKBi PA162381083.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0215.
HOGENOMi HOG000245250.
HOVERGENi HBG094012.
InParanoidi Q9HA77.
KOi K01883.
OMAi DKRHASD.
OrthoDBi EOG7XPZ5N.
PhylomeDBi Q9HA77.
TreeFami TF300384.

Enzyme and pathway databases

Reactomei REACT_15302. Mitochondrial tRNA aminoacylation.

Miscellaneous databases

ChiTaRSi CARS2. human.
GenomeRNAii 79587.
NextBioi 68590.
PROi Q9HA77.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9HA77.
Bgeei Q9HA77.
CleanExi HS_CARS2.
Genevestigatori Q9HA77.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00041. Cys_tRNA_synth.
InterProi IPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
PANTHERi PTHR10890. PTHR10890. 1 hit.
Pfami PF01406. tRNA-synt_1e. 1 hit.
[Graphical view ]
PRINTSi PR00983. TRNASYNTHCYS.
SUPFAMi SSF47323. SSF47323. 1 hit.
TIGRFAMsi TIGR00435. cysS. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  2. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-555.
    Tissue: Lung.
  4. "Identification of immuno-peptidmics that recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
    Shichijo S., Itoh K.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-564.
    Tissue: Colon adenocarcinoma.
  5. "Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS."
    Bonnefond L., Fender A., Rudinger-Thirion J., Giege R., Florentz C., Sissler M.
    Biochemistry 44:4805-4816(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYCM_HUMAN
AccessioniPrimary (citable) accession number: Q9HA77
Secondary accession number(s): Q8NI84, Q96IV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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