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Q9HA77

- SYCM_HUMAN

UniProt

Q9HA77 - SYCM_HUMAN

Protein

Probable cysteine--tRNA ligase, mitochondrial

Gene

CARS2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi78 – 781ZincBy similarity
    Metal bindingi257 – 2571ZincBy similarity
    Metal bindingi282 – 2821ZincBy similarity
    Metal bindingi286 – 2861ZincBy similarity
    Binding sitei320 – 3201ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-tRNA ligase activity Source: RefGenome
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cysteinyl-tRNA aminoacylation Source: RefGenome
    2. gene expression Source: Reactome
    3. tRNA aminoacylation for protein translation Source: Reactome

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_15302. Mitochondrial tRNA aminoacylation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable cysteine--tRNA ligase, mitochondrial (EC:6.1.1.16)
    Alternative name(s):
    Cysteinyl-tRNA synthetase
    Short name:
    CysRS
    Gene namesi
    Name:CARS2
    ORF Names:OK/SW-cl.10
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 13

    Organism-specific databases

    HGNCiHGNC:25695. CARS2.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. cytosol Source: RefGenome
    2. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162381083.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 564Probable cysteine--tRNA ligase, mitochondrialPRO_0000250741
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Proteomic databases

    MaxQBiQ9HA77.
    PaxDbiQ9HA77.
    PeptideAtlasiQ9HA77.
    PRIDEiQ9HA77.

    PTM databases

    PhosphoSiteiQ9HA77.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9HA77.
    BgeeiQ9HA77.
    CleanExiHS_CARS2.
    GenevestigatoriQ9HA77.

    Organism-specific databases

    HPAiHPA041776.
    HPA043935.

    Interactioni

    Protein-protein interaction databases

    BioGridi122730. 2 interactions.
    IntActiQ9HA77. 1 interaction.
    STRINGi9606.ENSP00000257347.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HA77.
    SMRiQ9HA77. Positions 52-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi80 – 9011"HIGH" regionAdd
    BLAST
    Motifi317 – 3215"KMSKS" region

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0215.
    HOGENOMiHOG000245250.
    HOVERGENiHBG094012.
    InParanoidiQ9HA77.
    KOiK01883.
    OMAiDKRHASD.
    OrthoDBiEOG7XPZ5N.
    PhylomeDBiQ9HA77.
    TreeFamiTF300384.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00041. Cys_tRNA_synth.
    InterProiIPR015803. Cys-tRNA-ligase.
    IPR024909. Cys-tRNA/MSH_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PANTHERiPTHR10890. PTHR10890. 1 hit.
    PfamiPF01406. tRNA-synt_1e. 1 hit.
    [Graphical view]
    PRINTSiPR00983. TRNASYNTHCYS.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    TIGRFAMsiTIGR00435. cysS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9HA77-1 [UniParc]FASTAAdd to Basket

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    MLRTTRGPGL GPPLLQAALG LGRAGWHWPA GRAASGGRGR AWLQPTGRET    50
    GVQVYNSLTG RKEPLIVAHA EAASWYSCGP TVYDHAHLGH ACSYVRFDII 100
    RRILTKVFGC SIVMVMGITD VDDKIIKRAN EMNISPASLA SLYEEDFKQD 150
    MAALKVLPPT VYLRVTENIP QIISFIEGII ARGNAYSTAK GNVYFDLKSR 200
    GDKYGKLVGV VPGPVGEPAD SDKRHASDFA LWKAAKPQEV FWASPWGPGR 250
    PGWHIECSAI ASMVFGSQLD IHSGGIDLAF PHHENEIAQC EVFHQCEQWG 300
    NYFLHSGHLH AKGKEEKMSK SLKNYITIKD FLKTFSPDVF RFFCLRSSYR 350
    SAIDYSDSAM LQAQQLLLGL GSFLEDARAY MKGQLACGSV REAMLWERLS 400
    STKRAVKAAL ADDFDTPRVV DAILGLAHHG NGQLRASLKE PEGPRSPAVF 450
    GAIISYFEQF FETVGISLAN QQYVSGDGSE ATLHGVVDEL VRFRQKVRQF 500
    ALAMPEATGD ARRQQLLERQ PLLEACDTLR RGLTAHGINI KDRSSTTSTW 550
    ELLDQRTKDQ KSAG 564
    Length:564
    Mass (Da):62,224
    Last modified:March 1, 2001 - v1
    Checksum:i92F8B615E6657D50
    GO

    Sequence cautioni

    The sequence BAB93499.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti182 – 20221RGNAY…KSRGD → SWERLFNGKRQCLLRSESLE ET in BAB93499. 1 PublicationCuratedAdd
    BLAST
    Sequence conflicti207 – 22317LVGVV…ADSDK → IGRRGPWSSPETSGLLTS in BAB93499. 1 PublicationCuratedAdd
    BLAST
    Sequence conflicti227 – 2271S → N in BAB93499. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti440 – 4401E → K.
    Corresponds to variant rs965189 [ dbSNP | Ensembl ].
    VAR_034523
    Natural varianti555 – 5551Q → P.1 Publication
    Corresponds to variant rs1043886 [ dbSNP | Ensembl ].
    VAR_034524

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK022180 mRNA. Translation: BAB13978.1.
    AL157820, AL139385 Genomic DNA. Translation: CAI16971.1.
    AL139385, AL157820 Genomic DNA. Translation: CAI17004.1.
    BC007220 mRNA. Translation: AAH07220.1.
    AB062436 mRNA. Translation: BAB93499.1. Different initiation.
    CCDSiCCDS9514.1.
    RefSeqiNP_078813.1. NM_024537.2.
    UniGeneiHs.508725.

    Genome annotation databases

    EnsembliENST00000257347; ENSP00000257347; ENSG00000134905.
    GeneIDi79587.
    KEGGihsa:79587.
    UCSCiuc001vrd.2. human.

    Polymorphism databases

    DMDMi74761587.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK022180 mRNA. Translation: BAB13978.1 .
    AL157820 , AL139385 Genomic DNA. Translation: CAI16971.1 .
    AL139385 , AL157820 Genomic DNA. Translation: CAI17004.1 .
    BC007220 mRNA. Translation: AAH07220.1 .
    AB062436 mRNA. Translation: BAB93499.1 . Different initiation.
    CCDSi CCDS9514.1.
    RefSeqi NP_078813.1. NM_024537.2.
    UniGenei Hs.508725.

    3D structure databases

    ProteinModelPortali Q9HA77.
    SMRi Q9HA77. Positions 52-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122730. 2 interactions.
    IntActi Q9HA77. 1 interaction.
    STRINGi 9606.ENSP00000257347.

    Chemistry

    DrugBanki DB00151. L-Cysteine.

    PTM databases

    PhosphoSitei Q9HA77.

    Polymorphism databases

    DMDMi 74761587.

    Proteomic databases

    MaxQBi Q9HA77.
    PaxDbi Q9HA77.
    PeptideAtlasi Q9HA77.
    PRIDEi Q9HA77.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000257347 ; ENSP00000257347 ; ENSG00000134905 .
    GeneIDi 79587.
    KEGGi hsa:79587.
    UCSCi uc001vrd.2. human.

    Organism-specific databases

    CTDi 79587.
    GeneCardsi GC13M111293.
    HGNCi HGNC:25695. CARS2.
    HPAi HPA041776.
    HPA043935.
    MIMi 612800. gene.
    neXtProti NX_Q9HA77.
    PharmGKBi PA162381083.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0215.
    HOGENOMi HOG000245250.
    HOVERGENi HBG094012.
    InParanoidi Q9HA77.
    KOi K01883.
    OMAi DKRHASD.
    OrthoDBi EOG7XPZ5N.
    PhylomeDBi Q9HA77.
    TreeFami TF300384.

    Enzyme and pathway databases

    Reactomei REACT_15302. Mitochondrial tRNA aminoacylation.

    Miscellaneous databases

    ChiTaRSi CARS2. human.
    GenomeRNAii 79587.
    NextBioi 68590.
    PROi Q9HA77.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9HA77.
    Bgeei Q9HA77.
    CleanExi HS_CARS2.
    Genevestigatori Q9HA77.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00041. Cys_tRNA_synth.
    InterProi IPR015803. Cys-tRNA-ligase.
    IPR024909. Cys-tRNA/MSH_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    PANTHERi PTHR10890. PTHR10890. 1 hit.
    Pfami PF01406. tRNA-synt_1e. 1 hit.
    [Graphical view ]
    PRINTSi PR00983. TRNASYNTHCYS.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    TIGRFAMsi TIGR00435. cysS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    2. "The DNA sequence and analysis of human chromosome 13."
      Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
      Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-555.
      Tissue: Lung.
    4. "Identification of immuno-peptidmics that recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
      Shichijo S., Itoh K.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-564.
      Tissue: Colon adenocarcinoma.
    5. "Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS."
      Bonnefond L., Fender A., Rudinger-Thirion J., Giege R., Florentz C., Sissler M.
      Biochemistry 44:4805-4816(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYCM_HUMAN
    AccessioniPrimary (citable) accession number: Q9HA77
    Secondary accession number(s): Q8NI84, Q96IV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Human chromosome 13
      Human chromosome 13: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3