Q9HA47 (UCK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uridine-cytidine kinase 1
Short name=UCK 1
EC=2.7.1.48

Alternative name(s):
Cytidine monophosphokinase 1
Uridine monophosphokinase 1

Gene names

Name:	UCK1
Synonyms:	URK1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.
Catalytic activity	ATP + uridine = ADP + UMP. ATP + cytidine = ADP + CMP.
Pathway	Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3. Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the uridine kinase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CTP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway UMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway pyrimidine nucleobase metabolic process Traceable author statement. Source: Reactome pyrimidine nucleoside salvage Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside kinase activity Inferred from experiment. Source: Reactome phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro uridine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9HA47-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9HA47-2)

The sequence of this isoform differs from the canonical sequence as follows:
     170-277: VLRDVRRGRD...SHLESSSRPH → DKEVCRCDHPARSGQYGCHQPDRAAHPGHSEW

Isoform 3 (identifier: Q9HA47-3)

The sequence of this isoform differs from the canonical sequence as follows:
     28-36: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 277

277

Uridine-cytidine kinase 1

PRO_0000164453

Regions

Nucleotide binding

30 – 38

9

ATP

Sites

Binding site

87

1

Substrate By similarity

Binding site

115

1

Substrate By similarity

Binding site

120

1

Substrate By similarity

Binding site

169

1

Substrate By similarity

Binding site

178

1

Substrate By similarity

Binding site

186

1

Substrate By similarity

Binding site

215

1

ATP

Natural variations

Alternative sequence

28 – 36

9

Missing in isoform 3.

VSP_045174

Alternative sequence

170 – 277

108

VLRDV…SSRPH → DKEVCRCDHPARSGQYGCHQ PDRAAHPGHSEW in isoform 2.

VSP_041269

Experimental info

Sequence conflict

8 – 17

10

DCESPAPEAD → GARARAGAN in AAL75943. Ref.3

Sequence conflict

56 – 57

2

QR → HG in AAL75943. Ref.3

Sequence conflict

175

1

R → H in CAG33562. Ref.5

Sequence conflict

247

1

S → T in AAL75943. Ref.3

Isoform 2:

Sequence conflict

180

1

A → T in AAH15547. Ref.8

Secondary structure

1

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277

Helix Strand Turn

Details...

Beta strand

23 – 29

7

Helix

36 – 46

11

Helix

49 – 51

3

Helix

54 – 56

3

Beta strand

58 – 63

6

Helix

64 – 67

4

Helix

73 – 80

8

Helix

89 – 91

3

Helix

94 – 105

12

Beta strand

110 – 112

3

Turn

117 – 120

4

Beta strand

127 – 129

3

Beta strand

133 – 138

6

Turn

140 – 143

4

Helix

146 – 149

4

Beta strand

153 – 159

7

Helix

162 – 173

12

Helix

180 – 189

10

Helix

191 – 198

8

Helix

200 – 205

6

Beta strand

207 – 213

7

Helix

217 – 232

16

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: AFD9ED92780CD502	FASTA	277	31,435
	10 20 30 40 50 60 MASAGGEDCE SPAPEADRPH QRPFLIGVSG GTASGKSTVC EKIMELLGQN EVEQRQRKVV 70 80 90 100 110 120 ILSQDRFYKV LTAEQKAKAL KGQYNFDHPD AFDNDLMHRT LKNIVEGKTV EVPTYDFVTH 130 140 150 160 170 180 SRLPETTVVY PADVVLFEGI LVFYSQEIRD MFHLRLFVDT DSDVRLSRRV LRDVRRGRDL 190 200 210 220 230 240 EQILTQYTTF VKPAFEEFCL PTKKYADVII PRGVDNMVAI NLIVQHIQDI LNGDICKWHR 250 260 270 GGSNGRSYKR TFSEPGDHPG MLTSGKRSHL ESSSRPH
	Isoform 2 [UniParc]. Checksum: 0E5F2F00FF6052D3	FASTA	201	22,761
	10 20 30 40 50 60 MASAGGEDCE SPAPEADRPH QRPFLIGVSG GTASGKSTVC EKIMELLGQN EVEQRQRKVV 70 80 90 100 110 120 ILSQDRFYKV LTAEQKAKAL KGQYNFDHPD AFDNDLMHRT LKNIVEGKTV EVPTYDFVTH 130 140 150 160 170 180 SRLPETTVVY PADVVLFEGI LVFYSQEIRD MFHLRLFVDT DSDVRLSRRD KEVCRCDHPA 190 200 RSGQYGCHQP DRAAHPGHSE W
	Isoform 3 [UniParc]. Checksum: 3944B9DCF2A0BAE3	FASTA	268	30,690
	10 20 30 40 50 60 MASAGGEDCE SPAPEADRPH QRPFLIGSTV CEKIMELLGQ NEVEQRQRKV VILSQDRFYK 70 80 90 100 110 120 VLTAEQKAKA LKGQYNFDHP DAFDNDLMHR TLKNIVEGKT VEVPTYDFVT HSRLPETTVV 130 140 150 160 170 180 YPADVVLFEG ILVFYSQEIR DMFHLRLFVD TDSDVRLSRR VLRDVRRGRD LEQILTQYTT 190 200 210 220 230 240 FVKPAFEEFC LPTKKYADVI IPRGVDNMVA INLIVQHIQD ILNGDICKWH RGGSNGRSYK 250 260 RTFSEPGDHP GMLTSGKRSH LESSSRPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases." Van Rompay A.R., Norda A., Linden K., Johansson M., Karlsson A. Mol. Pharmacol. 59:1181-1186(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
[2]	"Human uridine kinase from prostate cancer cell line (LNCaP)." Ho Y.S., Johnson R.K. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Cloning of a new human cDNA similar to Mus musculus uridine kinase mRNA." Xin Y.R., Yu L., Zhao S.Y. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Hippocampus and Mammary gland.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Colon.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Structure of human uridine-cytidine kinase 1." Northeast structural genomics consortium (NESG) Submitted (JAN-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 22-243 IN COMPLEX WITH ADP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF237290 mRNA. Translation: AAK28324.1.
AF254133 mRNA. Translation: AAK49122.1.
AF125106 mRNA. Translation: AAL75943.1.
AK022317 mRNA. Translation: BAB14010.1.
AK295471 mRNA. Translation: BAG58400.1.
CR457281 mRNA. Translation: CAG33562.1.
AL358781 Genomic DNA. Translation: CAI40228.1.
AL358781 Genomic DNA. Translation: CAI40229.1.
AL358781 Genomic DNA. Translation: CAI40231.1.
CH471090 Genomic DNA. Translation: EAW87983.1.
BC015547 mRNA. Translation: AAH15547.1.
BC091495 mRNA. Translation: AAH91495.1.

IPI

IPI00027771.
IPI00303595.

RefSeq

NP_001129426.1. NM_001135954.1.
NP_001248379.1. NM_001261450.1.
NP_001248380.1. NM_001261451.1.
NP_113620.1. NM_031432.2.

UniGene

Hs.9597.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JEO	X-ray	2.50	A	22-243	[»]
2UVQ	X-ray	3.00	A	22-243	[»]

ProteinModelPortal

Q9HA47.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9HA47. 2 interactions.

MINT

MINT-1392002.

STRING

9606.ENSP00000361289.

PTM databases

PhosphoSite

Q9HA47.

Polymorphism databases

DMDM

20455360.

Proteomic databases

PaxDb

Q9HA47.

PRIDE

Q9HA47.

Protocols and materials databases

DNASU

83549.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000372208; ENSP00000361282; ENSG00000130717.
ENST00000372210; ENSP00000361284; ENSG00000130717.
ENST00000372215; ENSP00000361289; ENSG00000130717.

GeneID

83549.

KEGG

hsa:83549.

UCSC

uc004cay.3. human.
uc004cba.3. human.
uc010mzk.3. human.

Organism-specific databases

CTD

83549.

GeneCards

GC09M134399.

HGNC

HGNC:14859. UCK1.

HPA

HPA050969.

MIM

609328. gene.

neXtProt

NX_Q9HA47.

PharmGKB

PA134861770.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0572.

HOGENOM

HOG000262756.

HOVERGEN

HBG023339.

KO

K00876.

OrthoDB

EOG4R7VBB.

PhylomeDB

Q9HA47.

Enzyme and pathway databases

BioCyc

MetaCyc:HS05429-MONOMER.

Reactome

REACT_111217. Metabolism.

SABIO-RK

Q9HA47.

UniPathway

UPA00574; UER00637.
UPA00579; UER00640.

Gene expression databases

ArrayExpress

Q9HA47.

Bgee

Q9HA47.

CleanEx

HS_UCK1.

Genevestigator

Q9HA47.

GermOnline

ENSG00000130717. Homo sapiens.

Family and domain databases

InterPro

IPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR000764. Uridine_kinase.
[Graphical view]

Pfam

PF00485. PRK. 1 hit.
[Graphical view]

PRINTS

PR00988. URIDINKINASE.

SUPFAM

SSF52540. SSF52540. 1 hit.

TIGRFAMs

TIGR00235. udk. 1 hit.

ProtoNet

Search...

Other

ChEMBL

CHEMBL5682.

ChiTaRS

UCK1. human.

EvolutionaryTrace

Q9HA47.

GenomeRNAi

83549.

NextBio

72489.

SOURCE

Search...

Entry information

Entry name

UCK1_HUMAN

Accession

Primary (citable) accession number: Q9HA47
Secondary accession number(s): Q5JT10 , Q5JT12, Q5JT13, Q6IA74, Q96BJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.