Q9H9Y6 (RPA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase I subunit RPA2
Short name=RNA polymerase I subunit 2
EC=2.7.7.6

Alternative name(s):
DNA-directed RNA polymerase I 135 kDa polypeptide
Short name=RPA135

Gene names

Name:

POLR1B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1135 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity. Ref.4
Subcellular location	Nucleus › nucleolus Ref.3.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryo development Inferred from electronic annotation. Source: Compara embryo implantation Inferred from electronic annotation. Source: Compara nucleologenesis Inferred from electronic annotation. Source: Compara rRNA transcription Inferred from electronic annotation. Source: Compara termination of RNA polymerase I transcription Traceable author statement. Source: Reactome transcription elongation from RNA polymerase I promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase I promoter Traceable author statement. Source: Reactome
Cellular_component	DNA-directed RNA polymerase I complex Inferred from electronic annotation. Source: Compara cytoplasm Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay. Source: HPA nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H9Y6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H9Y6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     61-116: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1135	1135	DNA-directed RNA polymerase I subunit RPA2		PRO_0000048072
Regions
	Zinc finger	1070 – 1101	32	C4-type Potential
Natural variations
	Alternative sequence	61 – 116	56	Missing in isoform 2.		VSP_017823
	Natural variant	295	1	S → L. Ref.1 Corresponds to variant rs1545133 [ dbSNP | Ensembl ].		VAR_034476
Experimental info
	Sequence conflict	247	1	L → R in BAB14082. Ref.1
	Sequence conflict	746	1	I → T in BAB14296. Ref.1
	Sequence conflict	800	1	D → G in BAC87247. Ref.1
	Sequence conflict	868	1	C → R in BAB14082. Ref.1
	Sequence conflict	887	1	H → R in BAC87247. Ref.1
	Sequence conflict	889	1	Q → R in BAB14082. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 4, 2006. Version 2. Checksum: 1F019E07F866C22E	FASTA	1,135	128,229
	10 20 30 40 50 60 MDPGSRWRNL PSGPSLKHLT DPSYGIPREQ QKAALQELTR AHVESFNYAV HEGLGLAVQA 70 80 90 100 110 120 IPPFEFAFKD ERISFTILDA VISPPTVPKG TICKEANVYP AECRGRRSTY RGKLTADINW 130 140 150 160 170 180 AVNGISKGII KQFLGYVPIM VKSKLCNLRN LPPQALIEHH EEAEEMGGYF IINGIEKVIR 190 200 210 220 230 240 MLIMPRRNFP IAMIRPKWKT RGPGYTQYGV SMHCVREEHS AVNMNLHYLE NGTVMLNFIY 250 260 270 280 290 300 RKELFFLPLG FALKALVSFS DYQIFQELIK GKEDDSFLRN SVSQMLRIVM EEGCSTQKQV 310 320 330 340 350 360 LNYLGECFRV KLNVPDWYPN EQAAEFLFNQ CICIHLKSNT EKFYMLCLMT RKLFALAKGE 370 380 390 400 410 420 CMEDNPDSLV NQEVLTPGQL FLMFLKEKLE GWLVSIKIAF DKKAQKTSVS MNTDNLMRIF 430 440 450 460 470 480 TMGIDLTKPF EYLFATGNLR SKTGLGLLQD SGLCVVADKL NFIRYLSHFR CVHRGADFAK 490 500 510 520 530 540 MRTTTVRRLL PESWGFLCPV HTPDGEPCGL MNHLTAVCEV VTQFVYTASI PALLCNLGVT 550 560 570 580 590 600 PIDGAPHRSY SECYPVLLDG VMVGWVDKDL APGIADSLRH FKVLREKRIP PWMEVVLIPM 610 620 630 640 650 660 TGKPSLYPGL FLFTTPCRLV RPVQNLALGK EELIGTMEQI FMNVAIFEDE VFAGVTTHQE 670 680 690 700 710 720 LFPHSLLSVI ANFIPFSDHN QSPRNMYQCQ MGKQTMGFPL LTYQDRSDNK LYRLQTPQSP 730 740 750 760 770 780 LVRPSMYDYY DMDNYPIGTN AIVAVISYTG YDMEDAMIVN KASWERGFAH GSVYKSEFID 790 800 810 820 830 840 LSEKIKQGDS SLVFGIKPGD PRVLQKLDDD GLPFIGAKLQ YGDPYYSYLN LNTGESFVMY 850 860 870 880 890 900 YKSKENCVVD NIKVCSNDTG SGKFKCVCIT MRVPRNPTIG DKFASRHGQK GILSRLWPAE 910 920 930 940 950 960 DMPFTESGMV PDILFNPHGF PSRMTIGMLI ESMAGKSAAL HGLCHDATPF IFSEENSALE 970 980 990 1000 1010 1020 YFGEMLKAAG YNFYGTERLY SGISGLELEA DIFIGVVYYQ RLRHMVSDKF QVRTTGARDR 1030 1040 1050 1060 1070 1080 VTNQPIGGRN VQGGIRFGEM ERDALLAHGT SFLLHDRLFN CSDRSVAHVC VKCGSLLSPL 1090 1100 1110 1120 1130 LEKPPPSWSA MRNRKYNCTL CSRSDTIDTV SVPYVFRYFV AELAAMNIKV KLDVV
	Isoform 2 [UniParc]. Checksum: FC7A4BB26405E435	FASTA	1,079	121,988
	10 20 30 40 50 60 MDPGSRWRNL PSGPSLKHLT DPSYGIPREQ QKAALQELTR AHVESFNYAV HEGLGLAVQA 70 80 90 100 110 120 DINWAVNGIS KGIIKQFLGY VPIMVKSKLC NLRNLPPQAL IEHHEEAEEM GGYFIINGIE 130 140 150 160 170 180 KVIRMLIMPR RNFPIAMIRP KWKTRGPGYT QYGVSMHCVR EEHSAVNMNL HYLENGTVML 190 200 210 220 230 240 NFIYRKELFF LPLGFALKAL VSFSDYQIFQ ELIKGKEDDS FLRNSVSQML RIVMEEGCST 250 260 270 280 290 300 QKQVLNYLGE CFRVKLNVPD WYPNEQAAEF LFNQCICIHL KSNTEKFYML CLMTRKLFAL 310 320 330 340 350 360 AKGECMEDNP DSLVNQEVLT PGQLFLMFLK EKLEGWLVSI KIAFDKKAQK TSVSMNTDNL 370 380 390 400 410 420 MRIFTMGIDL TKPFEYLFAT GNLRSKTGLG LLQDSGLCVV ADKLNFIRYL SHFRCVHRGA 430 440 450 460 470 480 DFAKMRTTTV RRLLPESWGF LCPVHTPDGE PCGLMNHLTA VCEVVTQFVY TASIPALLCN 490 500 510 520 530 540 LGVTPIDGAP HRSYSECYPV LLDGVMVGWV DKDLAPGIAD SLRHFKVLRE KRIPPWMEVV 550 560 570 580 590 600 LIPMTGKPSL YPGLFLFTTP CRLVRPVQNL ALGKEELIGT MEQIFMNVAI FEDEVFAGVT 610 620 630 640 650 660 THQELFPHSL LSVIANFIPF SDHNQSPRNM YQCQMGKQTM GFPLLTYQDR SDNKLYRLQT 670 680 690 700 710 720 PQSPLVRPSM YDYYDMDNYP IGTNAIVAVI SYTGYDMEDA MIVNKASWER GFAHGSVYKS 730 740 750 760 770 780 EFIDLSEKIK QGDSSLVFGI KPGDPRVLQK LDDDGLPFIG AKLQYGDPYY SYLNLNTGES 790 800 810 820 830 840 FVMYYKSKEN CVVDNIKVCS NDTGSGKFKC VCITMRVPRN PTIGDKFASR HGQKGILSRL 850 860 870 880 890 900 WPAEDMPFTE SGMVPDILFN PHGFPSRMTI GMLIESMAGK SAALHGLCHD ATPFIFSEEN 910 920 930 940 950 960 SALEYFGEML KAAGYNFYGT ERLYSGISGL ELEADIFIGV VYYQRLRHMV SDKFQVRTTG 970 980 990 1000 1010 1020 ARDRVTNQPI GGRNVQGGIR FGEMERDALL AHGTSFLLHD RLFNCSDRSV AHVCVKCGSL 1030 1040 1050 1060 1070 LSPLLEKPPP SWSAMRNRKY NCTLCSRSDT IDTVSVPYVF RYFVAELAAM NIKVKLDVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT LEU-295. Tissue: Testis.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"hRRN3 is essential in the SL1-mediated recruitment of RNA polymerase I to rRNA gene promoters." Miller G., Panov K.I., Friedrich J.K., Trinkle-Mulcahy L., Lamond A.I., Zomerdijk J.C.B.M. EMBO J. 20:1373-1382(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor." Panov K.I., Panova T.B., Gadal O., Nishiyama K., Saito T., Russell J., Zomerdijk J.C.B.M. Mol. Cell. Biol. 26:5436-5448(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK022533 mRNA. Translation: BAB14082.1. AK022890 mRNA. Translation: BAB14296.1. AK128044 mRNA. Translation: BAC87247.1. AC012442 Genomic DNA. Translation: AAX81999.1.
IPI	IPI00026445. IPI01010348.
RefSeq	NP_001131076.1. NM_001137604.1. NP_061887.2. NM_019014.4.
UniGene	Hs.741552.
3D structure databases
ProteinModelPortal	Q9H9Y6.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-27538N.
IntAct	Q9H9Y6. 18 interactions.
MINT	MINT-4727878.
STRING	9606.ENSP00000263331.
PTM databases
PhosphoSite	Q9H9Y6.
Polymorphism databases
DMDM	92090637.
2D gel databases
SWISS-2DPAGE	Q9H9Y6.
Proteomic databases
PaxDb	Q9H9Y6.
PRIDE	Q9H9Y6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263331; ENSP00000263331; ENSG00000125630. ENST00000417433; ENSP00000405358; ENSG00000125630.
GeneID	84172.
KEGG	hsa:84172.
UCSC	uc002thw.2. human. uc010fkn.2. human.
Organism-specific databases
CTD	84172.
GeneCards	GC02P113299.
H-InvDB	HIX0002380.
HGNC	HGNC:20454. POLR1B.
HPA	HPA037528.
MIM	602000. gene.
neXtProt	NX_Q9H9Y6.
PharmGKB	PA130601182.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0085.
HOVERGEN	HBG017742.
InParanoid	Q9H9Y6.
KO	K03002.
OrthoDB	EOG4H9XJP.
PhylomeDB	Q9H9Y6.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q9H9Y6.
Bgee	Q9H9Y6.
CleanEx	HS_POLR1B.
Genevestigator	Q9H9Y6.
GermOnline	ENSG00000125630. Homo sapiens.
Family and domain databases
Gene3D	2.40.270.10. 2 hits. 2.40.50.150. 1 hit.
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR009674. RNA_pol_Rpa2-specific. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
PANTHER	PTHR20856. PTHR20856. 1 hit.
Pfam	PF06883. RNA_pol_Rpa2_4. 1 hit. PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	84172.
NextBio	73536.
SOURCE	Search...

Entry information

Entry name

RPA2_HUMAN

Accession

Primary (citable) accession number: Q9H9Y6
Secondary accession number(s): Q585T5, Q6ZRR2, Q9H9D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	April 4, 2006
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.