Q9H9S3 (S61A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein transport protein Sec61 subunit alpha isoform 2
Short name=Sec61 alpha-2

Gene names

Name:

SEC61A2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins. Found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins By similarity.
Subunit structure	Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the SecY/SEC61-alpha family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Traceable author statement. Source: Reactome protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ribosome binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H9S3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H9S3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     416-476: YIPTAAAFGG...EVGGMGALFF → PKVKLRRWKGEGRHFTKRILFY

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9H9S3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     26-47: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Protein transport protein Sec61 subunit alpha isoform 2		PRO_0000131795
Regions
	Topological domain	1 – 33	33	Cytoplasmic Potential
	Transmembrane	34 – 53	20	Helical; Potential
	Topological domain	54 – 76	23	Lumenal Potential
	Transmembrane	77 – 96	20	Helical; Potential
	Topological domain	97 – 117	21	Cytoplasmic Potential
	Transmembrane	118 – 138	21	Helical; Potential
	Topological domain	139 – 144	6	Lumenal Potential
	Transmembrane	145 – 165	21	Helical; Potential
	Topological domain	166 – 172	7	Cytoplasmic Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Topological domain	194 – 240	47	Lumenal Potential
	Transmembrane	241 – 261	21	Helical; Potential
	Topological domain	262 – 288	27	Cytoplasmic Potential
	Transmembrane	289 – 309	21	Helical; Potential
	Topological domain	310 – 354	45	Lumenal Potential
	Transmembrane	355 – 375	21	Helical; Potential
	Topological domain	376 – 420	45	Cytoplasmic Potential
	Transmembrane	421 – 441	21	Helical; Potential
	Topological domain	442 – 445	4	Lumenal Potential
	Transmembrane	446 – 462	17	Helical; Potential
	Topological domain	463 – 476	14	Cytoplasmic Potential
Natural variations
	Alternative sequence	26 – 47	22	Missing in isoform 3.		VSP_046380
	Alternative sequence	416 – 476	61	YIPTA…GALFF → PKVKLRRWKGEGRHFTKRIL FY in isoform 2.		VSP_010472
Experimental info
	Sequence conflict	16	1	L → P in BAB14148. Ref.2
	Sequence conflict	133	1	M → I in BAB14148. Ref.2
	Sequence conflict	370	1	G → E in BAG63284. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 87CC8E7D366C2C37	FASTA	476	52,248
	10 20 30 40 50 60 MGIKFLEVIK PFCAVLPEIQ KPERKIQFRE KVLWTAITLF IFLVCCQIPL FGIMSSDSAD 70 80 90 100 110 120 PFYWMRVILA SNRGTLMELG ISPIVTSGLI MQLLAGAKII EVGDTPKDRA LFNGAQKLFG 130 140 150 160 170 180 MIITIGQAIV YVMTGMYGDP AEMGAGICLL IIIQLFVAGL IVLLLDELLQ KGYGLGSGIS 190 200 210 220 230 240 LFIATNICET IVWKAFSPTT INTGRGTEFE GAVIALFHLL ATRTDKVRAL REAFYRQNLP 250 260 270 280 290 300 NLMNLIATVF VFAVVIYFQG FRVDLPIKSA RYRGQYSSYP IKLFYTSNIP IILQSALVSN 310 320 330 340 350 360 LYVISQMLSV RFSGNFLVNL LGQWADVSGG GPARSYPVGG LCYYLSPPES MGAIFEDPVH 370 380 390 400 410 420 VVVYIIFMLG SCAFFSKTWI EVSGSSAKDV AKQLKEQQMV MRGHRDTSMV HELNRYIPTA 430 440 450 460 470 AAFGGLCIGA LSVLADFLGA IGSGTGILLA VTIIYQYFEI FVKEQAEVGG MGALFF
	Isoform 2 [UniParc]. Checksum: 6566C69D09393E35	FASTA	437	48,787
	10 20 30 40 50 60 MGIKFLEVIK PFCAVLPEIQ KPERKIQFRE KVLWTAITLF IFLVCCQIPL FGIMSSDSAD 70 80 90 100 110 120 PFYWMRVILA SNRGTLMELG ISPIVTSGLI MQLLAGAKII EVGDTPKDRA LFNGAQKLFG 130 140 150 160 170 180 MIITIGQAIV YVMTGMYGDP AEMGAGICLL IIIQLFVAGL IVLLLDELLQ KGYGLGSGIS 190 200 210 220 230 240 LFIATNICET IVWKAFSPTT INTGRGTEFE GAVIALFHLL ATRTDKVRAL REAFYRQNLP 250 260 270 280 290 300 NLMNLIATVF VFAVVIYFQG FRVDLPIKSA RYRGQYSSYP IKLFYTSNIP IILQSALVSN 310 320 330 340 350 360 LYVISQMLSV RFSGNFLVNL LGQWADVSGG GPARSYPVGG LCYYLSPPES MGAIFEDPVH 370 380 390 400 410 420 VVVYIIFMLG SCAFFSKTWI EVSGSSAKDV AKQLKEQQMV MRGHRDTSMV HELNRPKVKL 430 RRWKGEGRHF TKRILFY
	Isoform 3 [UniParc]. Checksum: B5FC2AE8AB2B312C	FASTA	454	49,593
	10 20 30 40 50 60 MGIKFLEVIK PFCAVLPEIQ KPERKIPLFG IMSSDSADPF YWMRVILASN RGTLMELGIS 70 80 90 100 110 120 PIVTSGLIMQ LLAGAKIIEV GDTPKDRALF NGAQKLFGMI ITIGQAIVYV MTGMYGDPAE 130 140 150 160 170 180 MGAGICLLII IQLFVAGLIV LLLDELLQKG YGLGSGISLF IATNICETIV WKAFSPTTIN 190 200 210 220 230 240 TGRGTEFEGA VIALFHLLAT RTDKVRALRE AFYRQNLPNL MNLIATVFVF AVVIYFQGFR 250 260 270 280 290 300 VDLPIKSARY RGQYSSYPIK LFYTSNIPII LQSALVSNLY VISQMLSVRF SGNFLVNLLG 310 320 330 340 350 360 QWADVSGGGP ARSYPVGGLC YYLSPPESMG AIFEDPVHVV VYIIFMLGSC AFFSKTWIEV 370 380 390 400 410 420 SGSSAKDVAK QLKEQQMVMR GHRDTSMVHE LNRYIPTAAA FGGLCIGALS VLADFLGAIG 430 440 450 SGTGILLAVT IIYQYFEIFV KEQAEVGGMG ALFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sec61 alpha isoforms." Finke K., Prehn S., Hartmann E. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Testis.
[3]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF346603 mRNA. Translation: AAK29084.1. AK022640 mRNA. Translation: BAB14148.1. AK292295 mRNA. Translation: BAF84984.1. AK301841 mRNA. Translation: BAG63284.1. AC073160 Genomic DNA. No translation available. CH471072 Genomic DNA. Translation: EAW86324.1.
IPI	IPI00182313. IPI00414427. IPI00414428.
RefSeq	NP_001136099.1. NM_001142627.1. NP_001136100.1. NM_001142628.1. NP_060614.2. NM_018144.3.
UniGene	Hs.112955.
3D structure databases
ProteinModelPortal	Q9H9S3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H9S3. 4 interactions.
STRING	9606.ENSP00000298428.
Protein family/group databases
TCDB	3.A.5.9.1. general secretory pathway (Sec) family.
PTM databases
PhosphoSite	Q9H9S3.
Polymorphism databases
DMDM	48477069.
Proteomic databases
PRIDE	Q9H9S3.
Protocols and materials databases
DNASU	55176.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000298428; ENSP00000298428; ENSG00000065665. ENST00000304267; ENSP00000302048; ENSG00000065665. ENST00000379033; ENSP00000368319; ENSG00000065665.
GeneID	55176.
KEGG	hsa:55176.
UCSC	uc001ile.2. human. uc001ilg.4. human. uc010qbq.1. human.
Organism-specific databases
CTD	55176.
GeneCards	GC10P012171.
HGNC	HGNC:17702. SEC61A2.
neXtProt	NX_Q9H9S3.
PharmGKB	PA134861382.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HOG000231249.
HOVERGEN	HBG055271.
KO	K10956.
OMA	GIVIYFQ.
PhylomeDB	Q9H9S3.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins. REACT_6900. Immune System. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q9H9S3.
Bgee	Q9H9S3.
CleanEx	HS_SEC61A2.
Genevestigator	Q9H9S3.
GermOnline	ENSG00000065665. Homo sapiens.
Family and domain databases
Gene3D	1.10.3370.10. 1 hit.
InterPro	IPR002208. SecY/SEC61-alpha. IPR023201. SecY_su_dom. IPR019561. Translocon_Sec61/SecY_plug_dom. [Graphical view]
PANTHER	PTHR10906. PTHR10906. 1 hit.
Pfam	PF10559. Plug_translocon. 1 hit. PF00344. SecY. 1 hit. [Graphical view]
PIRSF	PIRSF004557. SecY. 1 hit.
SUPFAM	SSF103491. SecY. 1 hit.
TIGRFAMs	TIGR00967. 3a0501s007. 1 hit.
PROSITE	PS00755. SECY_1. 1 hit. PS00756. SECY_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55176.
NextBio	58975.

Entry information

Entry name

S61A2_HUMAN

Accession

Primary (citable) accession number: Q9H9S3
Secondary accession number(s): A8K8D0, B4DX72, F8W773

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.