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Q9H9S3 (S61A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein Sec61 subunit alpha isoform 2

Short name=Sec61 alpha-2
Gene names
Name:SEC61A2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins. Found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins By similarity.

Subunit structure

Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the SecY/SEC61-alpha family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H9S3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H9S3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     416-476: YIPTAAAFGG...EVGGMGALFF → PKVKLRRWKGEGRHFTKRILFY
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9H9S3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     26-47: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 476475Protein transport protein Sec61 subunit alpha isoform 2
PRO_0000131795

Regions

Topological domain2 – 3332Cytoplasmic Potential
Transmembrane34 – 5320Helical; Potential
Topological domain54 – 7623Lumenal Potential
Transmembrane77 – 9620Helical; Potential
Topological domain97 – 11721Cytoplasmic Potential
Transmembrane118 – 13821Helical; Potential
Topological domain139 – 1446Lumenal Potential
Transmembrane145 – 16521Helical; Potential
Topological domain166 – 1727Cytoplasmic Potential
Transmembrane173 – 19321Helical; Potential
Topological domain194 – 24047Lumenal Potential
Transmembrane241 – 26121Helical; Potential
Topological domain262 – 28827Cytoplasmic Potential
Transmembrane289 – 30921Helical; Potential
Topological domain310 – 35445Lumenal Potential
Transmembrane355 – 37521Helical; Potential
Topological domain376 – 42045Cytoplasmic Potential
Transmembrane421 – 44121Helical; Potential
Topological domain442 – 4454Lumenal Potential
Transmembrane446 – 46217Helical; Potential
Topological domain463 – 47614Cytoplasmic Potential

Natural variations

Alternative sequence26 – 4722Missing in isoform 3.
VSP_046380
Alternative sequence416 – 47661YIPTA…GALFF → PKVKLRRWKGEGRHFTKRIL FY in isoform 2.
VSP_010472

Experimental info

Sequence conflict161L → P in BAB14148. Ref.2
Sequence conflict1331M → I in BAB14148. Ref.2
Sequence conflict3701G → E in BAG63284. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 87CC8E7D366C2C37

FASTA47652,248
        10         20         30         40         50         60 
MGIKFLEVIK PFCAVLPEIQ KPERKIQFRE KVLWTAITLF IFLVCCQIPL FGIMSSDSAD 

        70         80         90        100        110        120 
PFYWMRVILA SNRGTLMELG ISPIVTSGLI MQLLAGAKII EVGDTPKDRA LFNGAQKLFG 

       130        140        150        160        170        180 
MIITIGQAIV YVMTGMYGDP AEMGAGICLL IIIQLFVAGL IVLLLDELLQ KGYGLGSGIS 

       190        200        210        220        230        240 
LFIATNICET IVWKAFSPTT INTGRGTEFE GAVIALFHLL ATRTDKVRAL REAFYRQNLP 

       250        260        270        280        290        300 
NLMNLIATVF VFAVVIYFQG FRVDLPIKSA RYRGQYSSYP IKLFYTSNIP IILQSALVSN 

       310        320        330        340        350        360 
LYVISQMLSV RFSGNFLVNL LGQWADVSGG GPARSYPVGG LCYYLSPPES MGAIFEDPVH 

       370        380        390        400        410        420 
VVVYIIFMLG SCAFFSKTWI EVSGSSAKDV AKQLKEQQMV MRGHRDTSMV HELNRYIPTA 

       430        440        450        460        470 
AAFGGLCIGA LSVLADFLGA IGSGTGILLA VTIIYQYFEI FVKEQAEVGG MGALFF 

« Hide

Isoform 2 [UniParc].

Checksum: 6566C69D09393E35
Show »

FASTA43748,787
Isoform 3 [UniParc].

Checksum: B5FC2AE8AB2B312C
Show »

FASTA45449,593

References

« Hide 'large scale' references
[1]"Sec61 alpha isoforms."
Finke K., Prehn S., Hartmann E.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Testis.
[3]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF346603 mRNA. Translation: AAK29084.1.
AK022640 mRNA. Translation: BAB14148.1.
AK292295 mRNA. Translation: BAF84984.1.
AK301841 mRNA. Translation: BAG63284.1.
AC073160 Genomic DNA. No translation available.
CH471072 Genomic DNA. Translation: EAW86324.1.
RefSeqNP_001136099.1. NM_001142627.1.
NP_001136100.1. NM_001142628.1.
NP_060614.2. NM_018144.3.
UniGeneHs.112955.

3D structure databases

ProteinModelPortalQ9H9S3.
SMRQ9H9S3. Positions 1-476.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120475. 5 interactions.
IntActQ9H9S3. 4 interactions.
STRING9606.ENSP00000298428.

Protein family/group databases

TCDB3.A.5.9.1. the general secretory pathway (sec) family.

PTM databases

PhosphoSiteQ9H9S3.

Polymorphism databases

DMDM48477069.

Proteomic databases

PRIDEQ9H9S3.

Protocols and materials databases

DNASU55176.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000298428; ENSP00000298428; ENSG00000065665. [Q9H9S3-1]
ENST00000304267; ENSP00000302048; ENSG00000065665. [Q9H9S3-2]
ENST00000379033; ENSP00000368319; ENSG00000065665. [Q9H9S3-3]
GeneID55176.
KEGGhsa:55176.
UCSCuc001ile.2. human. [Q9H9S3-1]
uc001ilg.4. human. [Q9H9S3-2]

Organism-specific databases

CTD55176.
GeneCardsGC10P012171.
HGNCHGNC:17702. SEC61A2.
neXtProtNX_Q9H9S3.
PharmGKBPA134861382.
GenAtlasSearch...

Phylogenomic databases

HOGENOMHOG000231249.
HOVERGENHBG055271.
KOK10956.
OMANIQLWAR.
PhylomeDBQ9H9S3.
TreeFamTF300348.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
REACT_6900. Immune System.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ9H9S3.
BgeeQ9H9S3.
CleanExHS_SEC61A2.
GenevestigatorQ9H9S3.

Family and domain databases

Gene3D1.10.3370.10. 1 hit.
InterProIPR002208. SecY/SEC61-alpha.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]
PANTHERPTHR10906. PTHR10906. 1 hit.
PfamPF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]
PIRSFPIRSF004557. SecY. 1 hit.
SUPFAMSSF103491. SSF103491. 1 hit.
TIGRFAMsTIGR00967. 3a0501s007. 1 hit.
PROSITEPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi55176.
NextBio58975.
PROQ9H9S3.

Entry information

Entry nameS61A2_HUMAN
AccessionPrimary (citable) accession number: Q9H9S3
Secondary accession number(s): A8K8D0, B4DX72, F8W773
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM