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Protein

Protein transport protein Sec61 subunit alpha isoform 2

Gene

SEC61A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins. Found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiREACT_111178. ER-Phagosome pathway.
REACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_18273. XBP1(S) activates chaperone genes.

Protein family/group databases

TCDBi3.A.5.9.1. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein Sec61 subunit alpha isoform 2
Short name:
Sec61 alpha-2
Gene namesi
Name:SEC61A2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:17702. SEC61A2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 3332CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei34 – 5320HelicalSequence AnalysisAdd
BLAST
Topological domaini54 – 7623LumenalSequence AnalysisAdd
BLAST
Transmembranei77 – 9620HelicalSequence AnalysisAdd
BLAST
Topological domaini97 – 11721CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Topological domaini139 – 1446LumenalSequence Analysis
Transmembranei145 – 16521HelicalSequence AnalysisAdd
BLAST
Topological domaini166 – 1727CytoplasmicSequence Analysis
Transmembranei173 – 19321HelicalSequence AnalysisAdd
BLAST
Topological domaini194 – 24047LumenalSequence AnalysisAdd
BLAST
Transmembranei241 – 26121HelicalSequence AnalysisAdd
BLAST
Topological domaini262 – 28827CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei289 – 30921HelicalSequence AnalysisAdd
BLAST
Topological domaini310 – 35445LumenalSequence AnalysisAdd
BLAST
Transmembranei355 – 37521HelicalSequence AnalysisAdd
BLAST
Topological domaini376 – 42045CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei421 – 44121HelicalSequence AnalysisAdd
BLAST
Topological domaini442 – 4454LumenalSequence Analysis
Transmembranei446 – 46217HelicalSequence AnalysisAdd
BLAST
Topological domaini463 – 47614CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134861382.

Polymorphism and mutation databases

BioMutaiSEC61A2.
DMDMi48477069.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 476475Protein transport protein Sec61 subunit alpha isoform 2PRO_0000131795Add
BLAST

Proteomic databases

MaxQBiQ9H9S3.
PRIDEiQ9H9S3.

PTM databases

PhosphoSiteiQ9H9S3.

Expressioni

Gene expression databases

BgeeiQ9H9S3.
CleanExiHS_SEC61A2.
ExpressionAtlasiQ9H9S3. baseline and differential.
GenevestigatoriQ9H9S3.

Organism-specific databases

HPAiHPA045070.

Interactioni

Subunit structurei

Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma.By similarity

Protein-protein interaction databases

BioGridi120475. 4 interactions.
IntActiQ9H9S3. 4 interactions.
STRINGi9606.ENSP00000298428.

Structurei

3D structure databases

ProteinModelPortaliQ9H9S3.
SMRiQ9H9S3. Positions 25-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecY/SEC61-alpha family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003721.
HOGENOMiHOG000231249.
HOVERGENiHBG055271.
InParanoidiQ9H9S3.
KOiK10956.
OMAiWEIGIRE.
PhylomeDBiQ9H9S3.
TreeFamiTF300348.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
InterProiIPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]
PIRSFiPIRSF004557. SecY. 1 hit.
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H9S3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGIKFLEVIK PFCAVLPEIQ KPERKIQFRE KVLWTAITLF IFLVCCQIPL
60 70 80 90 100
FGIMSSDSAD PFYWMRVILA SNRGTLMELG ISPIVTSGLI MQLLAGAKII
110 120 130 140 150
EVGDTPKDRA LFNGAQKLFG MIITIGQAIV YVMTGMYGDP AEMGAGICLL
160 170 180 190 200
IIIQLFVAGL IVLLLDELLQ KGYGLGSGIS LFIATNICET IVWKAFSPTT
210 220 230 240 250
INTGRGTEFE GAVIALFHLL ATRTDKVRAL REAFYRQNLP NLMNLIATVF
260 270 280 290 300
VFAVVIYFQG FRVDLPIKSA RYRGQYSSYP IKLFYTSNIP IILQSALVSN
310 320 330 340 350
LYVISQMLSV RFSGNFLVNL LGQWADVSGG GPARSYPVGG LCYYLSPPES
360 370 380 390 400
MGAIFEDPVH VVVYIIFMLG SCAFFSKTWI EVSGSSAKDV AKQLKEQQMV
410 420 430 440 450
MRGHRDTSMV HELNRYIPTA AAFGGLCIGA LSVLADFLGA IGSGTGILLA
460 470
VTIIYQYFEI FVKEQAEVGG MGALFF
Length:476
Mass (Da):52,248
Last modified:January 23, 2007 - v3
Checksum:i87CC8E7D366C2C37
GO
Isoform 2 (identifier: Q9H9S3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-476: YIPTAAAFGG...EVGGMGALFF → PKVKLRRWKGEGRHFTKRILFY

Note: No experimental confirmation available.

Show »
Length:437
Mass (Da):48,787
Checksum:i6566C69D09393E35
GO
Isoform 3 (identifier: Q9H9S3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     26-47: Missing.

Note: No experimental confirmation available.

Show »
Length:454
Mass (Da):49,593
Checksum:iB5FC2AE8AB2B312C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161L → P in BAB14148 (PubMed:14702039).Curated
Sequence conflicti133 – 1331M → I in BAB14148 (PubMed:14702039).Curated
Sequence conflicti370 – 3701G → E in BAG63284 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei26 – 4722Missing in isoform 3. 1 PublicationVSP_046380Add
BLAST
Alternative sequencei416 – 47661YIPTA…GALFF → PKVKLRRWKGEGRHFTKRIL FY in isoform 2. 1 PublicationVSP_010472Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF346603 mRNA. Translation: AAK29084.1.
AK022640 mRNA. Translation: BAB14148.1.
AK292295 mRNA. Translation: BAF84984.1.
AK301841 mRNA. Translation: BAG63284.1.
AC073160 Genomic DNA. No translation available.
CH471072 Genomic DNA. Translation: EAW86324.1.
CCDSiCCDS44358.1. [Q9H9S3-2]
CCDS44359.1. [Q9H9S3-3]
CCDS7088.1. [Q9H9S3-1]
RefSeqiNP_001136099.1. NM_001142627.2. [Q9H9S3-2]
NP_001136100.1. NM_001142628.1. [Q9H9S3-3]
NP_060614.2. NM_018144.3. [Q9H9S3-1]
UniGeneiHs.112955.

Genome annotation databases

EnsembliENST00000298428; ENSP00000298428; ENSG00000065665. [Q9H9S3-1]
ENST00000304267; ENSP00000302048; ENSG00000065665. [Q9H9S3-2]
ENST00000379033; ENSP00000368319; ENSG00000065665. [Q9H9S3-3]
GeneIDi55176.
KEGGihsa:55176.
UCSCiuc001ile.2. human. [Q9H9S3-1]
uc001ilg.4. human. [Q9H9S3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF346603 mRNA. Translation: AAK29084.1.
AK022640 mRNA. Translation: BAB14148.1.
AK292295 mRNA. Translation: BAF84984.1.
AK301841 mRNA. Translation: BAG63284.1.
AC073160 Genomic DNA. No translation available.
CH471072 Genomic DNA. Translation: EAW86324.1.
CCDSiCCDS44358.1. [Q9H9S3-2]
CCDS44359.1. [Q9H9S3-3]
CCDS7088.1. [Q9H9S3-1]
RefSeqiNP_001136099.1. NM_001142627.2. [Q9H9S3-2]
NP_001136100.1. NM_001142628.1. [Q9H9S3-3]
NP_060614.2. NM_018144.3. [Q9H9S3-1]
UniGeneiHs.112955.

3D structure databases

ProteinModelPortaliQ9H9S3.
SMRiQ9H9S3. Positions 25-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120475. 4 interactions.
IntActiQ9H9S3. 4 interactions.
STRINGi9606.ENSP00000298428.

Protein family/group databases

TCDBi3.A.5.9.1. the general secretory pathway (sec) family.

PTM databases

PhosphoSiteiQ9H9S3.

Polymorphism and mutation databases

BioMutaiSEC61A2.
DMDMi48477069.

Proteomic databases

MaxQBiQ9H9S3.
PRIDEiQ9H9S3.

Protocols and materials databases

DNASUi55176.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298428; ENSP00000298428; ENSG00000065665. [Q9H9S3-1]
ENST00000304267; ENSP00000302048; ENSG00000065665. [Q9H9S3-2]
ENST00000379033; ENSP00000368319; ENSG00000065665. [Q9H9S3-3]
GeneIDi55176.
KEGGihsa:55176.
UCSCiuc001ile.2. human. [Q9H9S3-1]
uc001ilg.4. human. [Q9H9S3-2]

Organism-specific databases

CTDi55176.
GeneCardsiGC10P012171.
HGNCiHGNC:17702. SEC61A2.
HPAiHPA045070.
neXtProtiNX_Q9H9S3.
PharmGKBiPA134861382.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000003721.
HOGENOMiHOG000231249.
HOVERGENiHBG055271.
InParanoidiQ9H9S3.
KOiK10956.
OMAiWEIGIRE.
PhylomeDBiQ9H9S3.
TreeFamiTF300348.

Enzyme and pathway databases

ReactomeiREACT_111178. ER-Phagosome pathway.
REACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_18273. XBP1(S) activates chaperone genes.

Miscellaneous databases

ChiTaRSiSEC61A2. human.
GenomeRNAii55176.
NextBioi58975.
PROiQ9H9S3.

Gene expression databases

BgeeiQ9H9S3.
CleanExiHS_SEC61A2.
ExpressionAtlasiQ9H9S3. baseline and differential.
GenevestigatoriQ9H9S3.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
InterProiIPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]
PIRSFiPIRSF004557. SecY. 1 hit.
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sec61 alpha isoforms."
    Finke K., Prehn S., Hartmann E.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Tissue: Testis.
  3. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiS61A2_HUMAN
AccessioniPrimary (citable) accession number: Q9H9S3
Secondary accession number(s): A8K8D0, B4DX72, F8W773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.