ID   I20L2_HUMAN             Reviewed;         353 AA.
AC   Q9H9L3; D3DVC6; Q64KA2;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   24-JAN-2024, entry version 166.
DE   RecName: Full=Interferon-stimulated 20 kDa exonuclease-like 2;
DE            EC=3.1.-.-;
GN   Name=ISG20L2; ORFNames=HSD-38, HSD38;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RA   Yang C.B., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.;
RT   "A new spermatogenesis-related gene.";
RL   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA   Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA   Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA   Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA   Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA   Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA   Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA   Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA   Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA   Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA   Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA   Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA   Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA   McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA   Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA   White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA   Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA   Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA   Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=12429849; DOI=10.1091/mbc.e02-05-0271;
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   "Functional proteomic analysis of human nucleolus.";
RL   Mol. Biol. Cell 13:4100-4109(2002).
RN   [7]
RP   FUNCTION, CATALYTIC ACTIVITY, AND SUBCELLULAR LOCATION.
RX   PubMed=18065403; DOI=10.1074/mcp.m700510-mcp200;
RA   Coute Y., Kindbeiter K., Belin S., Dieckmann R., Duret L., Bezin L.,
RA   Sanchez J.-C., Diaz J.-J.;
RT   "ISG20L2, a novel vertebrate nucleolar exoribonuclease involved in ribosome
RT   biogenesis.";
RL   Mol. Cell. Proteomics 7:546-559(2008).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
CC   -!- FUNCTION: 3'-> 5'-exoribonuclease involved in ribosome biogenesis in
CC       the processing of the 12S pre-rRNA. Displays a strong specificity for a
CC       3'-end containing a free hydroxyl group. {ECO:0000269|PubMed:18065403}.
CC   -!- INTERACTION:
CC       Q9H9L3; O00423: EML1; NbExp=3; IntAct=EBI-751335, EBI-751327;
CC       Q9H9L3; P16333: NCK1; NbExp=2; IntAct=EBI-751335, EBI-389883;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12429849,
CC       ECO:0000269|PubMed:18065403}.
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DR   EMBL; AY391837; AAS55433.1; -; mRNA.
DR   EMBL; AK022733; BAB14212.1; -; mRNA.
DR   EMBL; AL590666; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471121; EAW52919.1; -; Genomic_DNA.
DR   EMBL; CH471121; EAW52920.1; -; Genomic_DNA.
DR   EMBL; BC000575; AAH00575.1; -; mRNA.
DR   CCDS; CCDS1153.1; -.
DR   RefSeq; NP_001290024.1; NM_001303095.1.
DR   RefSeq; XP_005245573.1; XM_005245516.4.
DR   RefSeq; XP_006711623.1; XM_006711560.3.
DR   RefSeq; XP_006711624.1; XM_006711561.3.
DR   AlphaFoldDB; Q9H9L3; -.
DR   SMR; Q9H9L3; -.
DR   BioGRID; 123618; 44.
DR   IntAct; Q9H9L3; 22.
DR   MINT; Q9H9L3; -.
DR   STRING; 9606.ENSP00000323424; -.
DR   iPTMnet; Q9H9L3; -.
DR   PhosphoSitePlus; Q9H9L3; -.
DR   SwissPalm; Q9H9L3; -.
DR   BioMuta; ISG20L2; -.
DR   DMDM; 42559527; -.
DR   EPD; Q9H9L3; -.
DR   jPOST; Q9H9L3; -.
DR   MassIVE; Q9H9L3; -.
DR   MaxQB; Q9H9L3; -.
DR   PaxDb; 9606-ENSP00000323424; -.
DR   PeptideAtlas; Q9H9L3; -.
DR   ProteomicsDB; 81328; -.
DR   Pumba; Q9H9L3; -.
DR   Antibodypedia; 20441; 49 antibodies from 20 providers.
DR   DNASU; 81875; -.
DR   Ensembl; ENST00000313146.10; ENSP00000323424.6; ENSG00000143319.17.
DR   Ensembl; ENST00000368219.2; ENSP00000357202.2; ENSG00000143319.17.
DR   GeneID; 81875; -.
DR   KEGG; hsa:81875; -.
DR   MANE-Select; ENST00000368219.2; ENSP00000357202.2; NM_001370150.2; NP_001357079.1.
DR   UCSC; uc001fps.2; human.
DR   AGR; HGNC:25745; -.
DR   CTD; 81875; -.
DR   GeneCards; ISG20L2; -.
DR   HGNC; HGNC:25745; ISG20L2.
DR   HPA; ENSG00000143319; Tissue enriched (testis).
DR   MIM; 611930; gene.
DR   neXtProt; NX_Q9H9L3; -.
DR   OpenTargets; ENSG00000143319; -.
DR   PharmGKB; PA142671650; -.
DR   VEuPathDB; HostDB:ENSG00000143319; -.
DR   eggNOG; KOG2249; Eukaryota.
DR   GeneTree; ENSGT00940000159724; -.
DR   HOGENOM; CLU_022453_0_0_1; -.
DR   InParanoid; Q9H9L3; -.
DR   OMA; PCPIVDY; -.
DR   OrthoDB; 5479236at2759; -.
DR   PhylomeDB; Q9H9L3; -.
DR   TreeFam; TF354340; -.
DR   PathwayCommons; Q9H9L3; -.
DR   Reactome; R-HSA-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   SignaLink; Q9H9L3; -.
DR   BioGRID-ORCS; 81875; 708 hits in 1152 CRISPR screens.
DR   ChiTaRS; ISG20L2; human.
DR   GenomeRNAi; 81875; -.
DR   Pharos; Q9H9L3; Tdark.
DR   PRO; PR:Q9H9L3; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   RNAct; Q9H9L3; Protein.
DR   Bgee; ENSG00000143319; Expressed in right testis and 199 other cell types or tissues.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000175; F:3'-5'-RNA exonuclease activity; IEA:InterPro.
DR   GO; GO:0004527; F:exonuclease activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR   GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR   CDD; cd06149; ISG20; 1.
DR   Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR037433; ISG20_DEDDh.
DR   InterPro; IPR047021; REXO1/3/4-like.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR12801:SF78; INTERFERON-STIMULATED 20 KDA EXONUCLEASE-LIKE 2; 1.
DR   PANTHER; PTHR12801; RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED; 1.
DR   Pfam; PF00929; RNase_T; 1.
DR   SMART; SM00479; EXOIII; 1.
DR   SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR   SWISS-2DPAGE; Q9H9L3; -.
DR   Genevisible; Q9H9L3; HS.
PE   1: Evidence at protein level;
KW   Exonuclease; Hydrolase; Nuclease; Nucleus; Reference proteome;
KW   Ribosome biogenesis.
FT   CHAIN           1..353
FT                   /note="Interferon-stimulated 20 kDa exonuclease-like 2"
FT                   /id="PRO_0000084121"
FT   DOMAIN          178..353
FT                   /note="Exonuclease"
FT   REGION          1..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          125..172
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        66..92
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        151..171
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         130
FT                   /note="N -> S (in dbSNP:rs3795737)"
FT                   /id="VAR_038171"
SQ   SEQUENCE   353 AA;  39154 MW;  07558CDED402C195 CRC64;
     MSTLLLNLDF GEPPPKKALE GNAKHRNFVK KRRLLERRGF LSKKNQPPSK APKLHSEPSK
     KGETPTVDGT WKTPSFPKKK TAASSNGSGQ PLDKKAAVSW LTPAPSKKAD SVAAKVDLLG
     EFQSALPKIN SHPTRSQKKS SQKKSSKKNH PQKNAPQNST QAHSENKCSG ASQKLPRKMV
     AIDCEMVGTG PKGHVSSLAR CSIVNYNGDV LYDEYILPPC HIVDYRTRWS GIRKQHMVNA
     TPFKIARGQI LKILTGKIVV GHAIHNDFKA LQYFHPKSLT RDTSHIPPLN RKADCPENAT
     MSLKHLTKKL LNRDIQVGKS GHSSVEDAQA TMELYKLVEV EWEEHLARNP PTD
//