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Protein

Interferon-stimulated 20 kDa exonuclease-like 2

Gene

ISG20L2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

3'-> 5'-exoribonuclease involved in ribosome biogenesis in the processing of the 12S pre-rRNA. Displays a strong specificity for a 3'-end containing a free hydroxyl group.1 Publication

GO - Molecular functioni

  • exonuclease activity Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Ribosome biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-stimulated 20 kDa exonuclease-like 2 (EC:3.1.-.-)
Gene namesi
Name:ISG20L2
ORF Names:HSD-38, HSD38
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:25745. ISG20L2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671650.

Polymorphism and mutation databases

BioMutaiISG20L2.
DMDMi42559527.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Interferon-stimulated 20 kDa exonuclease-like 2PRO_0000084121Add
BLAST

Proteomic databases

MaxQBiQ9H9L3.
PaxDbiQ9H9L3.
PeptideAtlasiQ9H9L3.
PRIDEiQ9H9L3.

2D gel databases

SWISS-2DPAGEQ9H9L3.

PTM databases

PhosphoSiteiQ9H9L3.

Expressioni

Gene expression databases

BgeeiQ9H9L3.
CleanExiHS_ISG20L2.
GenevestigatoriQ9H9L3.

Organism-specific databases

HPAiHPA028005.
HPA054285.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
EML1O004233EBI-751335,EBI-751327
NCK1P163332EBI-751335,EBI-389883

Protein-protein interaction databases

BioGridi123618. 3 interactions.
IntActiQ9H9L3. 9 interactions.
MINTiMINT-1457372.
STRINGi9606.ENSP00000323424.

Structurei

3D structure databases

ProteinModelPortaliQ9H9L3.
SMRiQ9H9L3. Positions 178-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini178 – 353176ExonucleaseAdd
BLAST

Sequence similaritiesi

Contains 1 exonuclease domain.Curated

Phylogenomic databases

eggNOGiCOG0847.
GeneTreeiENSGT00520000055542.
HOGENOMiHOG000182422.
HOVERGENiHBG107986.
InParanoidiQ9H9L3.
KOiK18329.
OMAiHMVNATP.
OrthoDBiEOG7WHHB0.
PhylomeDBiQ9H9L3.
TreeFamiTF354340.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9H9L3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLLLNLDF GEPPPKKALE GNAKHRNFVK KRRLLERRGF LSKKNQPPSK
60 70 80 90 100
APKLHSEPSK KGETPTVDGT WKTPSFPKKK TAASSNGSGQ PLDKKAAVSW
110 120 130 140 150
LTPAPSKKAD SVAAKVDLLG EFQSALPKIN SHPTRSQKKS SQKKSSKKNH
160 170 180 190 200
PQKNAPQNST QAHSENKCSG ASQKLPRKMV AIDCEMVGTG PKGHVSSLAR
210 220 230 240 250
CSIVNYNGDV LYDEYILPPC HIVDYRTRWS GIRKQHMVNA TPFKIARGQI
260 270 280 290 300
LKILTGKIVV GHAIHNDFKA LQYFHPKSLT RDTSHIPPLN RKADCPENAT
310 320 330 340 350
MSLKHLTKKL LNRDIQVGKS GHSSVEDAQA TMELYKLVEV EWEEHLARNP

PTD
Length:353
Mass (Da):39,154
Last modified:March 1, 2001 - v1
Checksum:i07558CDED402C195
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti130 – 1301N → S.
Corresponds to variant rs3795737 [ dbSNP | Ensembl ].
VAR_038171

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY391837 mRNA. Translation: AAS55433.1.
AK022733 mRNA. Translation: BAB14212.1.
AL590666 Genomic DNA. Translation: CAI16341.1.
CH471121 Genomic DNA. Translation: EAW52919.1.
CH471121 Genomic DNA. Translation: EAW52920.1.
BC000575 mRNA. Translation: AAH00575.1.
CCDSiCCDS1153.1.
RefSeqiNP_001290024.1. NM_001303095.1.
XP_005245573.1. XM_005245516.3.
XP_006711623.1. XM_006711560.2.
XP_006711624.1. XM_006711561.2.
UniGeneiHs.301904.

Genome annotation databases

EnsembliENST00000313146; ENSP00000323424; ENSG00000143319.
ENST00000368219; ENSP00000357202; ENSG00000143319.
GeneIDi81875.
KEGGihsa:81875.
UCSCiuc001fps.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY391837 mRNA. Translation: AAS55433.1.
AK022733 mRNA. Translation: BAB14212.1.
AL590666 Genomic DNA. Translation: CAI16341.1.
CH471121 Genomic DNA. Translation: EAW52919.1.
CH471121 Genomic DNA. Translation: EAW52920.1.
BC000575 mRNA. Translation: AAH00575.1.
CCDSiCCDS1153.1.
RefSeqiNP_001290024.1. NM_001303095.1.
XP_005245573.1. XM_005245516.3.
XP_006711623.1. XM_006711560.2.
XP_006711624.1. XM_006711561.2.
UniGeneiHs.301904.

3D structure databases

ProteinModelPortaliQ9H9L3.
SMRiQ9H9L3. Positions 178-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123618. 3 interactions.
IntActiQ9H9L3. 9 interactions.
MINTiMINT-1457372.
STRINGi9606.ENSP00000323424.

PTM databases

PhosphoSiteiQ9H9L3.

Polymorphism and mutation databases

BioMutaiISG20L2.
DMDMi42559527.

2D gel databases

SWISS-2DPAGEQ9H9L3.

Proteomic databases

MaxQBiQ9H9L3.
PaxDbiQ9H9L3.
PeptideAtlasiQ9H9L3.
PRIDEiQ9H9L3.

Protocols and materials databases

DNASUi81875.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313146; ENSP00000323424; ENSG00000143319.
ENST00000368219; ENSP00000357202; ENSG00000143319.
GeneIDi81875.
KEGGihsa:81875.
UCSCiuc001fps.1. human.

Organism-specific databases

CTDi81875.
GeneCardsiGC01M156691.
HGNCiHGNC:25745. ISG20L2.
HPAiHPA028005.
HPA054285.
MIMi611930. gene.
neXtProtiNX_Q9H9L3.
PharmGKBiPA142671650.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0847.
GeneTreeiENSGT00520000055542.
HOGENOMiHOG000182422.
HOVERGENiHBG107986.
InParanoidiQ9H9L3.
KOiK18329.
OMAiHMVNATP.
OrthoDBiEOG7WHHB0.
PhylomeDBiQ9H9L3.
TreeFamiTF354340.

Miscellaneous databases

ChiTaRSiISG20L2. human.
GenomeRNAii81875.
NextBioi72218.
PROiQ9H9L3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H9L3.
CleanExiHS_ISG20L2.
GenevestigatoriQ9H9L3.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new spermatogenesis-related gene."
    Yang C.B., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "ISG20L2, a novel vertebrate nucleolar exoribonuclease involved in ribosome biogenesis."
    Coute Y., Kindbeiter K., Belin S., Dieckmann R., Duret L., Bezin L., Sanchez J.-C., Diaz J.-J.
    Mol. Cell. Proteomics 7:546-559(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiI20L2_HUMAN
AccessioniPrimary (citable) accession number: Q9H9L3
Secondary accession number(s): D3DVC6, Q64KA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: March 1, 2001
Last modified: May 27, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.