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Q9H9L3 (I20L2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon-stimulated 20 kDa exonuclease-like 2

EC=3.1.-.-
Gene names
Name:ISG20L2
ORF Names:HSD-38, HSD38
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

3'-> 5'-exoribonuclease involved in ribosome biogenesis in the processing of the 12S pre-rRNA. Displays a strong specificity for a 3'-end containing a free hydroxyl group. Ref.7

Subcellular location

Nucleusnucleolus Ref.6 Ref.7.

Sequence similarities

Contains 1 exonuclease domain.

Ontologies

Keywords
   Biological processRibosome biogenesis
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   Molecular functionExonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processribosome biogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionexonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

poly(A) RNA binding

Inferred from direct assay PubMed 22658674. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 16189514PubMed 17474147PubMed 19060904. Source: IntAct

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Interferon-stimulated 20 kDa exonuclease-like 2
PRO_0000084121

Regions

Domain178 – 353176Exonuclease

Natural variations

Natural variant1301N → S.
Corresponds to variant rs3795737 [ dbSNP | Ensembl ].
VAR_038171

Sequences

Sequence LengthMass (Da)Tools
Q9H9L3 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 07558CDED402C195

FASTA35339,154
        10         20         30         40         50         60 
MSTLLLNLDF GEPPPKKALE GNAKHRNFVK KRRLLERRGF LSKKNQPPSK APKLHSEPSK 

        70         80         90        100        110        120 
KGETPTVDGT WKTPSFPKKK TAASSNGSGQ PLDKKAAVSW LTPAPSKKAD SVAAKVDLLG 

       130        140        150        160        170        180 
EFQSALPKIN SHPTRSQKKS SQKKSSKKNH PQKNAPQNST QAHSENKCSG ASQKLPRKMV 

       190        200        210        220        230        240 
AIDCEMVGTG PKGHVSSLAR CSIVNYNGDV LYDEYILPPC HIVDYRTRWS GIRKQHMVNA 

       250        260        270        280        290        300 
TPFKIARGQI LKILTGKIVV GHAIHNDFKA LQYFHPKSLT RDTSHIPPLN RKADCPENAT 

       310        320        330        340        350 
MSLKHLTKKL LNRDIQVGKS GHSSVEDAQA TMELYKLVEV EWEEHLARNP PTD 

« Hide

References

« Hide 'large scale' references
[1]"A new spermatogenesis-related gene."
Yang C.B., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.
Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Functional proteomic analysis of human nucleolus."
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"ISG20L2, a novel vertebrate nucleolar exoribonuclease involved in ribosome biogenesis."
Coute Y., Kindbeiter K., Belin S., Dieckmann R., Duret L., Bezin L., Sanchez J.-C., Diaz J.-J.
Mol. Cell. Proteomics 7:546-559(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY391837 mRNA. Translation: AAS55433.1.
AK022733 mRNA. Translation: BAB14212.1.
AL590666 Genomic DNA. Translation: CAI16341.1.
CH471121 Genomic DNA. Translation: EAW52919.1.
CH471121 Genomic DNA. Translation: EAW52920.1.
BC000575 mRNA. Translation: AAH00575.1.
CCDSCCDS1153.1.
RefSeqNP_112242.1. NM_030980.1.
XP_005245573.1. XM_005245516.2.
XP_005245574.1. XM_005245517.2.
XP_006711623.1. XM_006711560.1.
XP_006711624.1. XM_006711561.1.
UniGeneHs.301904.

3D structure databases

ProteinModelPortalQ9H9L3.
SMRQ9H9L3. Positions 178-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123618. 1 interaction.
IntActQ9H9L3. 7 interactions.
MINTMINT-1457372.
STRING9606.ENSP00000323424.

PTM databases

PhosphoSiteQ9H9L3.

Polymorphism databases

DMDM42559527.

2D gel databases

SWISS-2DPAGEQ9H9L3.

Proteomic databases

MaxQBQ9H9L3.
PaxDbQ9H9L3.
PeptideAtlasQ9H9L3.
PRIDEQ9H9L3.

Protocols and materials databases

DNASU81875.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000313146; ENSP00000323424; ENSG00000143319.
ENST00000368219; ENSP00000357202; ENSG00000143319.
GeneID81875.
KEGGhsa:81875.
UCSCuc001fps.1. human.

Organism-specific databases

CTD81875.
GeneCardsGC01M156691.
HGNCHGNC:25745. ISG20L2.
HPAHPA028005.
MIM611930. gene.
neXtProtNX_Q9H9L3.
PharmGKBPA142671650.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0847.
HOGENOMHOG000182422.
HOVERGENHBG107986.
InParanoidQ9H9L3.
KOK01175.
OMAHMVNATP.
OrthoDBEOG7WHHB0.
PhylomeDBQ9H9L3.
TreeFamTF354340.

Gene expression databases

BgeeQ9H9L3.
CleanExHS_ISG20L2.
GenevestigatorQ9H9L3.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
InterProIPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 1 hit.
ProtoNetSearch...

Other

ChiTaRSISG20L2. human.
GenomeRNAi81875.
NextBio72218.
PROQ9H9L3.
SOURCESearch...

Entry information

Entry nameI20L2_HUMAN
AccessionPrimary (citable) accession number: Q9H9L3
Secondary accession number(s): D3DVC6, Q64KA2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM