Q9H9G7 (AGO3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein argonaute-3
Short name=Argonaute3
Short name=hAgo3

Alternative name(s):
Eukaryotic translation initiation factor 2C 3
Short name=eIF-2C 3
Short name=eIF2C 3

Gene names

Name:	EIF2C3
Synonyms:	AGO3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	860 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Ref.6
Subunit structure	Interacts with EIF4B, IMP8, PRMT5 and TNRC6B. Ref.7
Subcellular location	Cytoplasm › P-body Ref.5.
Sequence similarities	Belongs to the argonaute family. Ago subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain.

Ontologies

Keywords
Biological process	RNA-mediated gene silencing Translation regulation
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA catabolic process Inferred from direct assay Ref.6. Source: UniProtKB negative regulation of translation involved in gene silencing by miRNA Inferred from direct assay Ref.6. Source: UniProtKB
Cellular component	cytoplasmic mRNA processing body Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.7. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IPO8	O15397	5	EBI-2267883,EBI-358808

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H9G7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H9G7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 860	860	Protein argonaute-3		PRO_0000194061
Regions
	Domain	236 – 349	114	PAZ
	Domain	518 – 819	302	Piwi
Natural variations
	Alternative sequence	1 – 234	234	Missing in isoform 2.		VSP_041084
Experimental info
	Sequence conflict	25	1	T → A in BAB14262. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: 6FF1277995E5322E	FASTA	860	97,360
	10 20 30 40 50 60 MEIGSAGPAG AQPLLMVPRR PGYGTMGKPI KLLANCFQVE IPKIDVYLYE VDIKPDKCPR 70 80 90 100 110 120 RVNREVVDSM VQHFKVTIFG DRRPVYDGKR SLYTANPLPV ATTGVDLDVT LPGEGGKDRP 130 140 150 160 170 180 FKVSIKFVSR VSWHLLHEVL TGRTLPEPLE LDKPISTNPV HAVDVVLRHL PSMKYTPVGR 190 200 210 220 230 240 SFFSAPEGYD HPLGGGREVW FGFHQSVRPA MWKMMLNIDV SATAFYKAQP VIQFMCEVLD 250 260 270 280 290 300 IHNIDEQPRP LTDSHRVKFT KEIKGLKVEV THCGTMRRKY RVCNVTRRPA SHQTFPLQLE 310 320 330 340 350 360 NGQTVERTVA QYFREKYTLQ LKYPHLPCLQ VGQEQKHTYL PLEVCNIVAG QRCIKKLTDN 370 380 390 400 410 420 QTSTMIKATA RSAPDRQEEI SRLVRSANYE TDPFVQEFQF KVRDEMAHVT GRVLPAPMLQ 430 440 450 460 470 480 YGGRNRTVAT PSHGVWDMRG KQFHTGVEIK MWAIACFATQ RQCREEILKG FTDQLRKISK 490 500 510 520 530 540 DAGMPIQGQP CFCKYAQGAD SVEPMFRHLK NTYSGLQLII VILPGKTPVY AEVKRVGDTL 550 560 570 580 590 600 LGMATQCVQV KNVIKTSPQT LSNLCLKINV KLGGINNILV PHQRPSVFQQ PVIFLGADVT 610 620 630 640 650 660 HPPAGDGKKP SIAAVVGSMD AHPSRYCATV RVQRPRQEII QDLASMVREL LIQFYKSTRF 670 680 690 700 710 720 KPTRIIFYRD GVSEGQFRQV LYYELLAIRE ACISLEKDYQ PGITYIVVQK RHHTRLFCAD 730 740 750 760 770 780 RTERVGRSGN IPAGTTVDTD ITHPYEFDFY LCSHAGIQGT SRPSHYHVLW DDNCFTADEL 790 800 810 820 830 840 QLLTYQLCHT YVRCTRSVSI PAPAYYAHLV AFRARYHLVD KEHDSAEGSH VSGQSNGRDP 850 860 QALAKAVQIH QDTLRTMYFA
	Isoform 2 [UniParc]. Checksum: 5475FD0337AAD9CE	FASTA	626	71,211
	10 20 30 40 50 60 MCEVLDIHNI DEQPRPLTDS HRVKFTKEIK GLKVEVTHCG TMRRKYRVCN VTRRPASHQT 70 80 90 100 110 120 FPLQLENGQT VERTVAQYFR EKYTLQLKYP HLPCLQVGQE QKHTYLPLEV CNIVAGQRCI 130 140 150 160 170 180 KKLTDNQTST MIKATARSAP DRQEEISRLV RSANYETDPF VQEFQFKVRD EMAHVTGRVL 190 200 210 220 230 240 PAPMLQYGGR NRTVATPSHG VWDMRGKQFH TGVEIKMWAI ACFATQRQCR EEILKGFTDQ 250 260 270 280 290 300 LRKISKDAGM PIQGQPCFCK YAQGADSVEP MFRHLKNTYS GLQLIIVILP GKTPVYAEVK 310 320 330 340 350 360 RVGDTLLGMA TQCVQVKNVI KTSPQTLSNL CLKINVKLGG INNILVPHQR PSVFQQPVIF 370 380 390 400 410 420 LGADVTHPPA GDGKKPSIAA VVGSMDAHPS RYCATVRVQR PRQEIIQDLA SMVRELLIQF 430 440 450 460 470 480 YKSTRFKPTR IIFYRDGVSE GQFRQVLYYE LLAIREACIS LEKDYQPGIT YIVVQKRHHT 490 500 510 520 530 540 RLFCADRTER VGRSGNIPAG TTVDTDITHP YEFDFYLCSH AGIQGTSRPS HYHVLWDDNC 550 560 570 580 590 600 FTADELQLLT YQLCHTYVRC TRSVSIPAPA YYAHLVAFRA RYHLVDKEHD SAEGSHVSGQ 610 620 SNGRDPQALA KAVQIHQDTL RTMYFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Leukocyte.
[4]	"Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs." Meister G., Landthaler M., Patkaniowska A., Dorsett Y., Teng G., Tuschl T. Mol. Cell 15:185-197(2004) [PubMed: 15260970] [Abstract] Cited for: ASSOCIATION WITH MIRNA.
[5]	"Inhibition of translational initiation by Let-7 MicroRNA in human cells." Pillai R.S., Bhattacharyya S.N., Artus C.G., Zoller T., Cougot N., Basyuk E., Bertrand E., Filipowicz W. Science 309:1573-1576(2005) [PubMed: 16081698] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Importance of translation and nonnucleolytic ago proteins for on-target RNA interference." Wu L., Fan J., Belasco J.G. Curr. Biol. 18:1327-1332(2008) [PubMed: 18771919] [Abstract] Cited for: FUNCTION.
[7]	"Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs." Weinmann L., Hoeck J., Ivacevic T., Ohrt T., Muetze J., Schwille P., Kremmer E., Benes V., Urlaub H., Meister G. Cell 136:496-507(2009) [PubMed: 19167051] [Abstract] Cited for: INTERACTION WITH EIF4B; IMP8; PRMT5 AND TNRC6B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK022827 mRNA. Translation: BAB14262.1. AL139286, AL138787 Genomic DNA. Translation: CAI22802.1. AL138787, AL139286 Genomic DNA. Translation: CAI22268.1. BC025769 mRNA. No translation available.
IPI	IPI00304181. IPI00551009.
RefSeq	NP_079128.2. NM_024852.3.
UniGene	Hs.657659.
3D structure databases
ProteinModelPortal	Q9H9G7.
SMR	Q9H9G7. Positions 223-389, 441-859.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H9G7. 47 interactions.
STRING	Q9H9G7.
PTM databases
PhosphoSite	Q9H9G7.
Polymorphism databases
DMDM	76803660.
Proteomic databases
PRIDE	Q9H9G7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373191; ENSP00000362287; ENSG00000126070.
GeneID	192669.
KEGG	hsa:192669.
UCSC	uc001bzp.1. human.
Organism-specific databases
CTD	192669.
GeneCards	GC01P036396.
HGNC	HGNC:18421. EIF2C3.
MIM	607355. gene.
neXtProt	NX_Q9H9G7.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12419.
GeneTree	ENSGT00550000074185.
HOGENOM	HBG717005.
InParanoid	Q9H9G7.
OMA	NWACINF.
OrthoDB	EOG4229J0.
PhylomeDB	Q9H9G7.
Enzyme and pathway databases
Reactome	REACT_12472. Regulatory RNA pathways.
Gene expression databases
ArrayExpress	Q9H9G7.
Bgee	Q9H9G7.
CleanEx	HS_EIF2C3.
Genevestigator	Q9H9G7.
GermOnline	ENSG00000126070. Homo sapiens.
Family and domain databases
InterPro	IPR014811. DUF1785. IPR003100. PAZ. IPR003165. Piwi. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K11593.
Pfam	PF08699. DUF1785. 1 hit. PF02170. PAZ. 1 hit. PF02171. Piwi. 1 hit. [Graphical view]
SMART	SM00949. PAZ. 1 hit. SM00950. Piwi. 1 hit. [Graphical view]
SUPFAM	SSF101690. PAZ. 1 hit. SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS50821. PAZ. 1 hit. PS50822. PIWI. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	89367.
SOURCE	Search...

Entry information

Entry name

AGO3_HUMAN

Accession

Primary (citable) accession number: Q9H9G7
Secondary accession number(s): Q5TA55, Q9H1U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	September 27, 2005
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.