Q9H9A5 (CNOTA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCR4-NOT transcription complex subunit 10

Gene names

Name:

CNOT10

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the CCR4-NOT complex at least composed of CNOT1, CNOT2, CNOT3, CNOT8, CNOT9, CNOT10 and TNKS1BP1 By similarity.
Sequence similarities	Belongs to the CNOT10 family.
Sequence caution	The sequence BAB13876.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear-transcribed mRNA poly(A) tail shortening Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CNOT6	Q9ULM6	1	EBI-1054261,EBI-2104530
CNOT6L	Q96LI5	1	EBI-1054261,EBI-1046635
CNOT8	Q9UFF9	1	EBI-1054261,EBI-742299
TOB1	P50616	1	EBI-1054261,EBI-723281

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H9A5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H9A5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     144-144: Missing.

Isoform 3 (identifier: Q9H9A5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     505-532: SSKSHDGDKFIPAPPSSPLRKQELENLK → R

Isoform 4 (identifier: Q9H9A5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     505-532: SSKSHDGDKFIPAPPSSPLRKQELENLK → R
     556-577: Missing.

Isoform 5 (identifier: Q9H9A5-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-293: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 744	743	CCR4-NOT transcription complex subunit 10		PRO_0000314579
Regions
	Coiled coil	74 – 107	34	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.5
Natural variations
	Alternative sequence	1 – 293	293	Missing in isoform 5.		VSP_030311
	Alternative sequence	144	1	Missing in isoform 2.		VSP_030312
	Alternative sequence	505 – 532	28	SSKSH…LENLK → R in isoform 3 and isoform 4.		VSP_030313
	Alternative sequence	556 – 577	22	Missing in isoform 4.		VSP_030314
	Natural variant	348	1	T → S. Corresponds to variant rs11558687 [ dbSNP | Ensembl ].		VAR_053982
	Natural variant	736	1	P → S. Ref.3 Corresponds to variant rs17849684 [ dbSNP | Ensembl ].		VAR_037957
Experimental info
	Sequence conflict	5	1	K → R in BAB14108. Ref.1
	Sequence conflict	52	1	C → R in BAB14478. Ref.1
	Sequence conflict	270	1	V → M in BAB13876. Ref.1
	Sequence conflict	478	1	D → G in BAB14108. Ref.1
	Sequence conflict	509	1	H → Y in BAB14108. Ref.1
	Sequence conflict	622	1	A → T in BAB14478. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BA1984B71F0A1E7A	FASTA	744	82,310
	10 20 30 40 50 60 MAADKPADQG AEKHEGTGQS SGITDQEKEL STNAFQAFTS GNYDACLQHL ACLQDINKDD 70 80 90 100 110 120 YKIILNTAVA EFFKSNQTTT DNLRQTLNQL KNQVHSAVEE MDGLDDVENS MLYYNQAVIL 130 140 150 160 170 180 YHLRQYTEAI SVGEKLYQFI EPFEEKFAQA VCFLLVDLYI LTYQAEKALH LLAVLEKMIS 190 200 210 220 230 240 QGNNNKNGKN ETGNNNNKDG SNHKAESGAL IEAAKSKIHQ YKVRAYIQMK SLKACKREIK 250 260 270 280 290 300 SVMNTAGNSA PSLFLKSNFE YLRGNYRKAV KLLNSSNIAE HPGFMKTGEC LRCMFWNNLG 310 320 330 340 350 360 CIHFAMSKHN LGIFYFKKAL QENDNVCAQL SAGSTDPGKK FSGRPMCTLL TNKRYELLYN 370 380 390 400 410 420 CGIQLLHIGR PLAAFECLIE AVQVYHANPR LWLRLAECCI AANKGTSEQE TKGLPSKKGI 430 440 450 460 470 480 VQSIVGQGYH RKIVLASQSI QNTVYNDGQS SAIPVASMEF AAICLRNALL LLPEEQQDPK 490 500 510 520 530 540 QENGAKNSNQ LGGNTESSES SETCSSKSHD GDKFIPAPPS SPLRKQELEN LKCSILACSA 550 560 570 580 590 600 YVALALGDNL MALNHADKLL QQPKLSGSLK FLGHLYAAEA LISLDRISDA ITHLNPENVT 610 620 630 640 650 660 DVSLGISSNE QDQGSDKGEN EAMESSGKRA PQCYPSSVNS ARTVMLFNLG SAYCLRSEYD 670 680 690 700 710 720 KARKCLHQAA SMIHPKEVPP EAILLAVYLE LQNGNTQLAL QIIKRNQLLP AVKTHSEVRK 730 740 KPVFQPVHPI QPIQMPAFTT VQRK
	Isoform 2 [UniParc]. Checksum: 46D04260B4E124E7	FASTA	743	82,181
	10 20 30 40 50 60 MAADKPADQG AEKHEGTGQS SGITDQEKEL STNAFQAFTS GNYDACLQHL ACLQDINKDD 70 80 90 100 110 120 YKIILNTAVA EFFKSNQTTT DNLRQTLNQL KNQVHSAVEE MDGLDDVENS MLYYNQAVIL 130 140 150 160 170 180 YHLRQYTEAI SVGEKLYQFI EPFEKFAQAV CFLLVDLYIL TYQAEKALHL LAVLEKMISQ 190 200 210 220 230 240 GNNNKNGKNE TGNNNNKDGS NHKAESGALI EAAKSKIHQY KVRAYIQMKS LKACKREIKS 250 260 270 280 290 300 VMNTAGNSAP SLFLKSNFEY LRGNYRKAVK LLNSSNIAEH PGFMKTGECL RCMFWNNLGC 310 320 330 340 350 360 IHFAMSKHNL GIFYFKKALQ ENDNVCAQLS AGSTDPGKKF SGRPMCTLLT NKRYELLYNC 370 380 390 400 410 420 GIQLLHIGRP LAAFECLIEA VQVYHANPRL WLRLAECCIA ANKGTSEQET KGLPSKKGIV 430 440 450 460 470 480 QSIVGQGYHR KIVLASQSIQ NTVYNDGQSS AIPVASMEFA AICLRNALLL LPEEQQDPKQ 490 500 510 520 530 540 ENGAKNSNQL GGNTESSESS ETCSSKSHDG DKFIPAPPSS PLRKQELENL KCSILACSAY 550 560 570 580 590 600 VALALGDNLM ALNHADKLLQ QPKLSGSLKF LGHLYAAEAL ISLDRISDAI THLNPENVTD 610 620 630 640 650 660 VSLGISSNEQ DQGSDKGENE AMESSGKRAP QCYPSSVNSA RTVMLFNLGS AYCLRSEYDK 670 680 690 700 710 720 ARKCLHQAAS MIHPKEVPPE AILLAVYLEL QNGNTQLALQ IIKRNQLLPA VKTHSEVRKK 730 740 PVFQPVHPIQ PIQMPAFTTV QRK
	Isoform 3 [UniParc]. Checksum: 4981E1632AB0368B	FASTA	717	79,377
	10 20 30 40 50 60 MAADKPADQG AEKHEGTGQS SGITDQEKEL STNAFQAFTS GNYDACLQHL ACLQDINKDD 70 80 90 100 110 120 YKIILNTAVA EFFKSNQTTT DNLRQTLNQL KNQVHSAVEE MDGLDDVENS MLYYNQAVIL 130 140 150 160 170 180 YHLRQYTEAI SVGEKLYQFI EPFEEKFAQA VCFLLVDLYI LTYQAEKALH LLAVLEKMIS 190 200 210 220 230 240 QGNNNKNGKN ETGNNNNKDG SNHKAESGAL IEAAKSKIHQ YKVRAYIQMK SLKACKREIK 250 260 270 280 290 300 SVMNTAGNSA PSLFLKSNFE YLRGNYRKAV KLLNSSNIAE HPGFMKTGEC LRCMFWNNLG 310 320 330 340 350 360 CIHFAMSKHN LGIFYFKKAL QENDNVCAQL SAGSTDPGKK FSGRPMCTLL TNKRYELLYN 370 380 390 400 410 420 CGIQLLHIGR PLAAFECLIE AVQVYHANPR LWLRLAECCI AANKGTSEQE TKGLPSKKGI 430 440 450 460 470 480 VQSIVGQGYH RKIVLASQSI QNTVYNDGQS SAIPVASMEF AAICLRNALL LLPEEQQDPK 490 500 510 520 530 540 QENGAKNSNQ LGGNTESSES SETCRCSILA CSAYVALALG DNLMALNHAD KLLQQPKLSG 550 560 570 580 590 600 SLKFLGHLYA AEALISLDRI SDAITHLNPE NVTDVSLGIS SNEQDQGSDK GENEAMESSG 610 620 630 640 650 660 KRAPQCYPSS VNSARTVMLF NLGSAYCLRS EYDKARKCLH QAASMIHPKE VPPEAILLAV 670 680 690 700 710 YLELQNGNTQ LALQIIKRNQ LLPAVKTHSE VRKKPVFQPV HPIQPIQMPA FTTVQRK
	Isoform 4 [UniParc]. Checksum: DEA9C9A66A253FE1	FASTA	695	76,968
	10 20 30 40 50 60 MAADKPADQG AEKHEGTGQS SGITDQEKEL STNAFQAFTS GNYDACLQHL ACLQDINKDD 70 80 90 100 110 120 YKIILNTAVA EFFKSNQTTT DNLRQTLNQL KNQVHSAVEE MDGLDDVENS MLYYNQAVIL 130 140 150 160 170 180 YHLRQYTEAI SVGEKLYQFI EPFEEKFAQA VCFLLVDLYI LTYQAEKALH LLAVLEKMIS 190 200 210 220 230 240 QGNNNKNGKN ETGNNNNKDG SNHKAESGAL IEAAKSKIHQ YKVRAYIQMK SLKACKREIK 250 260 270 280 290 300 SVMNTAGNSA PSLFLKSNFE YLRGNYRKAV KLLNSSNIAE HPGFMKTGEC LRCMFWNNLG 310 320 330 340 350 360 CIHFAMSKHN LGIFYFKKAL QENDNVCAQL SAGSTDPGKK FSGRPMCTLL TNKRYELLYN 370 380 390 400 410 420 CGIQLLHIGR PLAAFECLIE AVQVYHANPR LWLRLAECCI AANKGTSEQE TKGLPSKKGI 430 440 450 460 470 480 VQSIVGQGYH RKIVLASQSI QNTVYNDGQS SAIPVASMEF AAICLRNALL LLPEEQQDPK 490 500 510 520 530 540 QENGAKNSNQ LGGNTESSES SETCRCSILA CSAYVALALG DNLMALNHAE ALISLDRISD 550 560 570 580 590 600 AITHLNPENV TDVSLGISSN EQDQGSDKGE NEAMESSGKR APQCYPSSVN SARTVMLFNL 610 620 630 640 650 660 GSAYCLRSEY DKARKCLHQA ASMIHPKEVP PEAILLAVYL ELQNGNTQLA LQIIKRNQLL 670 680 690 PAVKTHSEVR KKPVFQPVHP IQPIQMPAFT TVQRK
	Isoform 5 [UniParc]. Checksum: 8F1BA3511B5863D5	FASTA	451	49,483
	10 20 30 40 50 60 MFWNNLGCIH FAMSKHNLGI FYFKKALQEN DNVCAQLSAG STDPGKKFSG RPMCTLLTNK 70 80 90 100 110 120 RYELLYNCGI QLLHIGRPLA AFECLIEAVQ VYHANPRLWL RLAECCIAAN KGTSEQETKG 130 140 150 160 170 180 LPSKKGIVQS IVGQGYHRKI VLASQSIQNT VYNDGQSSAI PVASMEFAAI CLRNALLLLP 190 200 210 220 230 240 EEQQDPKQEN GAKNSNQLGG NTESSESSET CSSKSHDGDK FIPAPPSSPL RKQELENLKC 250 260 270 280 290 300 SILACSAYVA LALGDNLMAL NHADKLLQQP KLSGSLKFLG HLYAAEALIS LDRISDAITH 310 320 330 340 350 360 LNPENVTDVS LGISSNEQDQ GSDKGENEAM ESSGKRAPQC YPSSVNSART VMLFNLGSAY 370 380 390 400 410 420 CLRSEYDKAR KCLHQAASMI HPKEVPPEAI LLAVYLELQN GNTQLALQII KRNQLLPAVK 430 440 450 THSEVRKKPV FQPVHPIQPI QMPAFTTVQR K

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 85-744 (ISOFORM 4). Tissue: Embryo.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT SER-736. Tissue: Skin.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 482-744 (ISOFORM 1). Tissue: Testis.
[5]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK021695 mRNA. Translation: BAB13876.1. Different initiation. AK022576 mRNA. Translation: BAB14108.1. AK022952 mRNA. Translation: BAB14327.1. AK023227 mRNA. Translation: BAB14478.1. AK027026 mRNA. Translation: BAB15629.1. CH471055 Genomic DNA. Translation: EAW64435.1. BC002928 mRNA. Translation: AAH02928.1. BC002931 mRNA. Translation: AAH02931.1. AL117639 mRNA. Translation: CAB56027.2.
IPI	IPI00396314. IPI00791933. IPI00794753. IPI00878368. IPI01014565.
RefSeq	NP_056257.1. NM_015442.1.
UniGene	Hs.444851.
3D structure databases
ProteinModelPortal	Q9H9A5.
SMR	Q9H9A5. Positions 29-70, 112-138, 356-401, 642-705.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46839N.
IntAct	Q9H9A5. 6 interactions.
STRING	Q9H9A5.
PTM databases
PhosphoSite	Q9H9A5.
Polymorphism databases
DMDM	74733982.
Proteomic databases
PRIDE	Q9H9A5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000328834; ENSP00000330060; ENSG00000182973.
GeneID	25904.
KEGG	hsa:25904.
UCSC	uc003cfc.1. human. uc003cfd.1. human. uc003cfe.1. human.
Organism-specific databases
CTD	25904.
GeneCards	GC03P032701.
H-InvDB	HIX0018971.
HGNC	HGNC:23817. CNOT10.
HPA	HPA035614.
neXtProt	NX_Q9H9A5.
PharmGKB	PA134862503.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17557.
GeneTree	ENSGT00390000001827.
HOVERGEN	HBG077464.
InParanoid	Q9H9A5.
OMA	KTLSEMR.
OrthoDB	EOG4CG07N.
PhylomeDB	Q9H9A5.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q9H9A5.
Bgee	Q9H9A5.
CleanEx	HS_CNOT10.
Genevestigator	Q9H9A5.
Family and domain databases
InterPro	IPR001440. TPR-1. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 4 hits.
KO	K12607.
Pfam	PF00515. TPR_1. 1 hit. [Graphical view]
SMART	SM00028. TPR. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	47378.

Entry information

Entry name

CNOTA_HUMAN

Accession

Primary (citable) accession number: Q9H9A5
Secondary accession number(s): Q9BU30 , Q9H5J7, Q9H8X1, Q9H9W0, Q9HAH3, Q9UFJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.