Q9H981 (ARP8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Actin-related protein 8
Short name=hArp8

Alternative name(s):
INO80 complex subunit N

Gene names

Name:	ACTR8
Synonyms:	ARP8, INO80N

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the functional organization of mitotic chromosomes. Ref.7 Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA reoplication and probably DNA repair. Required for the recruitment of INO80 (and probably the INO80 complex) to sites of DNA damage. Ref.7
Subunit structure	Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Interacts with ACTR5; the interaction is observed in asynchronous (interphase) cells but not in metaphase-arrested cells indicative for a possible dissociation of the INO80 complex in mitotic cells. Ref.7
Subcellular location	Nucleus. Chromosome. Note: Specifically localizes to mitotic chromosomes. Ref.7
Sequence similarities	Belongs to the actin family. ARP8 subfamily.
Sequence caution	The sequence BAB15402.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair Mitosis Transcription Transcription regulation
Cellular component	Chromosome Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Ino80 complex Inferred from direct assay Ref.10. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.7Ref.8. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ACTR5	Q9H9F9	2	EBI-769597,EBI-769418
UCHL5	Q9Y5K5	3	EBI-769597,EBI-1051183

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H981-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H981-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9H981-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-250: Missing.
     305-354: LCLAYGGSDVSRCFYWLMQRAGFPYRECQLTNKMDCLLLQHLKETFCHLD → IFSWN

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	Actin-related protein 8		PRO_0000089123
Amino acid modifications
	Modified residue	132	1	Phosphoserine Ref.5 Ref.6
	Modified residue	412	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	1 – 250	250	Missing in isoform 3.		VSP_040506
	Alternative sequence	1 – 111	111	Missing in isoform 2.		VSP_040507
	Alternative sequence	305 – 354	50	LCLAY…FCHLD → IFSWN in isoform 3.		VSP_040508
	Natural variant	56	1	T → I. Corresponds to variant rs3733082 [ dbSNP | Ensembl ].		VAR_028033
Experimental info
	Sequence conflict	596	1	D → G in BAB14352. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: DA71C49B9D2C4E19	FASTA	624	70,484
	10 20 30 40 50 60 MTQAEKGDTE NGKEKGGEKE KEQRGVKRPI VPALVPESLQ EQIQSNFIIV IHPGSTTLRI 70 80 90 100 110 120 GRATDTLPAS IPHVIARRHK QQGQPLYKDS WLLREGLNKP ESNEQRQNGL KMVDQAIWSK 130 140 150 160 170 180 KMSNGTRRIP VSPEQARSYN KQMRPAILDH CSGNKWTNTS HHPEYLVGEE ALYVNPLDCY 190 200 210 220 230 240 NIHWPIRRGQ LNIHPGPGGS LTAVLADIEV IWSHAIQKYL EIPLKDLKYY RCILLIPDIY 250 260 270 280 290 300 NKQHVKELVN MILMKMGFSG IVVHQESVCA TYGSGLSSTC IVDVGDQKTS VCCVEDGVSH 310 320 330 340 350 360 RNTRLCLAYG GSDVSRCFYW LMQRAGFPYR ECQLTNKMDC LLLQHLKETF CHLDQDISGL 370 380 390 400 410 420 QDHEFQIRHP DSPALLYQFR LGDEKLQAPM ALFYPATFGI VGQKMTTLQH RSQGDPEDPH 430 440 450 460 470 480 DEHYLLATQS KQEQSAKATA DRKSASKPIG FEGDLRGQSS DLPERLHSQE VDLGSAQGDG 490 500 510 520 530 540 LMAGNDSEEA LTALMSRKTA ISLFEGKALG LDKAILHSID CCSSDDTKKK MYSSILVVGG 550 560 570 580 590 600 GLMFHKAQEF LQHRILNKMP PSFRRIIENV DVITRPKDMD PRLIAWKGGA VLACLDTTQE 610 620 LWIYQREWQR FGVRMLRERA AFVW
	Isoform 2 [UniParc]. Checksum: 601862886A522A00	FASTA	513	58,045
	10 20 30 40 50 60 MVDQAIWSKK MSNGTRRIPV SPEQARSYNK QMRPAILDHC SGNKWTNTSH HPEYLVGEEA 70 80 90 100 110 120 LYVNPLDCYN IHWPIRRGQL NIHPGPGGSL TAVLADIEVI WSHAIQKYLE IPLKDLKYYR 130 140 150 160 170 180 CILLIPDIYN KQHVKELVNM ILMKMGFSGI VVHQESVCAT YGSGLSSTCI VDVGDQKTSV 190 200 210 220 230 240 CCVEDGVSHR NTRLCLAYGG SDVSRCFYWL MQRAGFPYRE CQLTNKMDCL LLQHLKETFC 250 260 270 280 290 300 HLDQDISGLQ DHEFQIRHPD SPALLYQFRL GDEKLQAPMA LFYPATFGIV GQKMTTLQHR 310 320 330 340 350 360 SQGDPEDPHD EHYLLATQSK QEQSAKATAD RKSASKPIGF EGDLRGQSSD LPERLHSQEV 370 380 390 400 410 420 DLGSAQGDGL MAGNDSEEAL TALMSRKTAI SLFEGKALGL DKAILHSIDC CSSDDTKKKM 430 440 450 460 470 480 YSSILVVGGG LMFHKAQEFL QHRILNKMPP SFRRIIENVD VITRPKDMDP RLIAWKGGAV 490 500 510 LACLDTTQEL WIYQREWQRF GVRMLRERAA FVW
	Isoform 3 [UniParc]. Checksum: 5A83E55423F44021	FASTA	329	36,737
	10 20 30 40 50 60 MILMKMGFSG IVVHQESVCA TYGSGLSSTC IVDVGDQKTS VCCVEDGVSH RNTRIFSWNQ 70 80 90 100 110 120 DISGLQDHEF QIRHPDSPAL LYQFRLGDEK LQAPMALFYP ATFGIVGQKM TTLQHRSQGD 130 140 150 160 170 180 PEDPHDEHYL LATQSKQEQS AKATADRKSA SKPIGFEGDL RGQSSDLPER LHSQEVDLGS 190 200 210 220 230 240 AQGDGLMAGN DSEEALTALM SRKTAISLFE GKALGLDKAI LHSIDCCSSD DTKKKMYSSI 250 260 270 280 290 300 LVVGGGLMFH KAQEFLQHRI LNKMPPSFRR IIENVDVITR PKDMDPRLIA WKGGAVLACL 310 320 DTTQELWIYQ REWQRFGVRM LRERAAFVW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain cortex and Small intestine.
[2]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed: 16641997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[4]	"A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex." Jin J., Cai Y., Yao T., Gottschalk A.J., Florens L., Swanson S.K., Gutierrez J.L., Coleman M.K., Workman J.L., Mushegian A., Washburn M.P., Conaway R.C., Conaway J.W. J. Biol. Chem. 280:41207-41212(2005) [PubMed: 16230350] [Abstract] Cited for: IDENTIFICATION IN INO80 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"The actin-related protein hArp8 accumulates on the mitotic chromosomes and functions in chromosome alignment." Aoyama N., Oka A., Kitayama K., Kurumizaka H., Harata M. Exp. Cell Res. 314:859-868(2008) [PubMed: 18163988] [Abstract] Cited for: FUNCTION IN MITOSIS, INTERACTION WITH ACTR5, SUBCELLULAR LOCATION.
[8]	"Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex." Yao T., Song L., Jin J., Cai Y., Takahashi H., Swanson S.K., Washburn M.P., Florens L., Conaway R.C., Cohen R.E., Conaway J.W. Mol. Cell 31:909-917(2008) [PubMed: 18922472] [Abstract] Cited for: IDENTIFICATION IN THE INO80 COMPLEX, MASS SPECTROMETRY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Subunit organization of the human INO80 chromatin remodeling complex: An evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling." Chen L., Cai Y., Jin J., Florens L., Swanson S.K., Washburn M.P., Conaway J.W., Conaway R.C. J. Biol. Chem. 286:11283-11289(2011) [PubMed: 21303910] [Abstract] Cited for: IDENTIFICATION IN THE INO80 COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK022996 mRNA. Translation: BAB14352.1. AK094507 mRNA. Translation: BAG52879.1. AK026232 mRNA. Translation: BAB15402.1. Different initiation. AC012467 Genomic DNA. No translation available. BC032744 mRNA. Translation: AAH32744.1.
IPI	IPI00025646. IPI00166798. IPI00794608.
RefSeq	NP_075050.3. NM_022899.4.
UniGene	Hs.412186.
3D structure databases
ProteinModelPortal	Q9H981.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H981. 14 interactions.
STRING	Q9H981.
PTM databases
PhosphoSite	Q9H981.
Polymorphism databases
DMDM	116241257.
Proteomic databases
PRIDE	Q9H981.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000335754; ENSP00000336842; ENSG00000113812.
GeneID	93973.
KEGG	hsa:93973.
UCSC	uc003dhc.1. human.
Organism-specific databases
CTD	93973.
GeneCards	GC03M053877.
H-InvDB	HIX0003379.
HGNC	HGNC:14672. ACTR8.
neXtProt	NX_Q9H981.
PharmGKB	PA24491.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000001763.
HOGENOM	HBG315884.
HOVERGEN	HBG023939.
InParanoid	Q9H981.
OMA	PKDMDPR.
OrthoDB	EOG4SXNC6.
PhylomeDB	Q9H981.
Gene expression databases
ArrayExpress	Q9H981.
Bgee	Q9H981.
CleanEx	HS_ACTR8.
Genevestigator	Q9H981.
GermOnline	ENSG00000113812. Homo sapiens.
Family and domain databases
InterPro	IPR004000. Actin-like. [Graphical view]
KO	K11673.
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	78228.

Entry information

Entry name

ARP8_HUMAN

Accession

Primary (citable) accession number: Q9H981
Secondary accession number(s): B3KSW7, Q8N566, Q9H663

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	October 17, 2006
Last modified:	December 14, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.