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Protein

Queuine tRNA-ribosyltransferase subunit QTRTD1

Gene

QTRTD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei272 – 2721NucleophileUniRule annotation
Metal bindingi351 – 3511ZincUniRule annotation
Metal bindingi353 – 3531ZincUniRule annotation
Metal bindingi356 – 3561ZincUniRule annotation
Metal bindingi382 – 3821ZincUniRule annotation

GO - Molecular functioni

  1. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferase subunit QTRTD1UniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Queuine tRNA-ribosyltransferase domain-containing protein 1UniRule annotation
Gene namesi
Name:QTRTD1UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:25771. QTRTD1.

Subcellular locationi

Cytoplasm UniRule annotation. Mitochondrion UniRule annotation
Note: May associate with the mitochondrion outer membrane.UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134925851.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Queuine tRNA-ribosyltransferase subunit QTRTD1PRO_0000295633Add
BLAST

Proteomic databases

MaxQBiQ9H974.
PaxDbiQ9H974.
PRIDEiQ9H974.

PTM databases

PhosphoSiteiQ9H974.

Expressioni

Gene expression databases

BgeeiQ9H974.
CleanExiHS_QTRTD1.
ExpressionAtlasiQ9H974. baseline and differential.
GenevestigatoriQ9H974.

Organism-specific databases

HPAiHPA036827.
HPA036828.

Interactioni

Subunit structurei

Interacts with QTRT1.UniRule annotation

Protein-protein interaction databases

BioGridi122812. 9 interactions.
IntActiQ9H974. 3 interactions.
STRINGi9606.ENSP00000281273.

Structurei

3D structure databases

ProteinModelPortaliQ9H974.
SMRiQ9H974. Positions 70-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0343.
GeneTreeiENSGT00530000063679.
HOGENOMiHOG000243329.
HOVERGENiHBG057274.
InParanoidiQ9H974.
KOiK15407.
OMAiMHNFEHY.
PhylomeDBiQ9H974.
TreeFamiTF317105.

Family and domain databases

Gene3Di3.20.20.105. 2 hits.
HAMAPiMF_03043. QTRD1.
InterProiIPR028592. QTRTD1.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 2 hits.
TIGRFAMsiTIGR00449. tgt_general. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H974-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLSLTKVVN GCRLGKIKNL GKTGDHTMDI PGCLLYTKTG SAPHLTHHTL
60 70 80 90 100
HNIHGVPAMA QLTLSSLAEH HEVLTEYKEG VGKFIGMPES LLYCSLHDPV
110 120 130 140 150
SPCPAGYVTN KSVSVWSVAG RVEMTVSKFM AIQKALQPDW FQCLSDGEVS
160 170 180 190 200
CKEATSIKRV RKSVDRSLLF LDNCLRLQEE SEVLQKSVII GVIEGGDVME
210 220 230 240 250
ERLRSARETA KRPVGGFLLD GFQGNPTTLE ARLRLLSSVT AELPEDKPRL
260 270 280 290 300
ISGVSRPDEV LECIERGVDL FESFFPYQVT ERGCALTFSF DYQPNPEETL
310 320 330 340 350
LQQNGTQEEI KCMDQIKKIE TTGCNQEITS FEINLKEKKY QEDFNPLVRG
360 370 380 390 400
CSCYCCKNHT RAYIHHLLVT NELLAGVLLM MHNFEHYFGF FHYIREALKS
410
DKLAQLKELI HRQAS
Length:415
Mass (Da):46,713
Last modified:March 1, 2001 - v1
Checksum:iC9447FD900DD667B
GO
Isoform 2 (identifier: Q9H974-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Show »
Length:292
Mass (Da):33,471
Checksum:iDCFC6BF2B6F7C601
GO
Isoform 3 (identifier: Q9H974-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: MKLSLTKVVN...PCPAGYVTNK → MVCWQ

Show »
Length:309
Mass (Da):35,377
Checksum:iA2A2F97FE37E120D
GO
Isoform 4 (identifier: Q9H974-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVCWQDLEESLRM

Note: No experimental confirmation available.

Show »
Length:427
Mass (Da):48,203
Checksum:i3D4B55C405EDDAD5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti246 – 2461D → G in CAG33577 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 123123Missing in isoform 2. 1 PublicationVSP_045140Add
BLAST
Alternative sequencei1 – 111111MKLSL…YVTNK → MVCWQ in isoform 3. 1 PublicationVSP_045141Add
BLAST
Alternative sequencei1 – 11M → MVCWQDLEESLRM in isoform 4. 1 PublicationVSP_046859

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023022 mRNA. Translation: BAB14361.1.
AK296938 mRNA. Translation: BAH12458.1.
AK299313 mRNA. Translation: BAH12998.1.
CR457296 mRNA. Translation: CAG33577.1.
AL833709 mRNA. No translation available.
AC092896 Genomic DNA. No translation available.
AC128687 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79613.1.
BC034559 mRNA. Translation: AAH34559.1.
BC039265 mRNA. Translation: AAH39265.1.
CCDSiCCDS33828.1. [Q9H974-1]
CCDS58844.1. [Q9H974-4]
CCDS58845.1. [Q9H974-3]
CCDS58846.1. [Q9H974-2]
RefSeqiNP_001243764.1. NM_001256835.1. [Q9H974-4]
NP_001243765.1. NM_001256836.1. [Q9H974-3]
NP_001243766.1. NM_001256837.1. [Q9H974-2]
NP_078914.1. NM_024638.3. [Q9H974-1]
XP_005247827.1. XM_005247770.1. [Q9H974-2]
UniGeneiHs.477162.

Genome annotation databases

EnsembliENST00000281273; ENSP00000281273; ENSG00000151576. [Q9H974-1]
ENST00000479882; ENSP00000418056; ENSG00000151576. [Q9H974-2]
ENST00000485050; ENSP00000420682; ENSG00000151576. [Q9H974-4]
ENST00000493014; ENSP00000419169; ENSG00000151576. [Q9H974-3]
GeneIDi79691.
KEGGihsa:79691.
UCSCiuc003eay.4. human. [Q9H974-1]
uc011bir.3. human.

Polymorphism databases

DMDMi74752736.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023022 mRNA. Translation: BAB14361.1.
AK296938 mRNA. Translation: BAH12458.1.
AK299313 mRNA. Translation: BAH12998.1.
CR457296 mRNA. Translation: CAG33577.1.
AL833709 mRNA. No translation available.
AC092896 Genomic DNA. No translation available.
AC128687 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79613.1.
BC034559 mRNA. Translation: AAH34559.1.
BC039265 mRNA. Translation: AAH39265.1.
CCDSiCCDS33828.1. [Q9H974-1]
CCDS58844.1. [Q9H974-4]
CCDS58845.1. [Q9H974-3]
CCDS58846.1. [Q9H974-2]
RefSeqiNP_001243764.1. NM_001256835.1. [Q9H974-4]
NP_001243765.1. NM_001256836.1. [Q9H974-3]
NP_001243766.1. NM_001256837.1. [Q9H974-2]
NP_078914.1. NM_024638.3. [Q9H974-1]
XP_005247827.1. XM_005247770.1. [Q9H974-2]
UniGeneiHs.477162.

3D structure databases

ProteinModelPortaliQ9H974.
SMRiQ9H974. Positions 70-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122812. 9 interactions.
IntActiQ9H974. 3 interactions.
STRINGi9606.ENSP00000281273.

PTM databases

PhosphoSiteiQ9H974.

Polymorphism databases

DMDMi74752736.

Proteomic databases

MaxQBiQ9H974.
PaxDbiQ9H974.
PRIDEiQ9H974.

Protocols and materials databases

DNASUi79691.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000281273; ENSP00000281273; ENSG00000151576. [Q9H974-1]
ENST00000479882; ENSP00000418056; ENSG00000151576. [Q9H974-2]
ENST00000485050; ENSP00000420682; ENSG00000151576. [Q9H974-4]
ENST00000493014; ENSP00000419169; ENSG00000151576. [Q9H974-3]
GeneIDi79691.
KEGGihsa:79691.
UCSCiuc003eay.4. human. [Q9H974-1]
uc011bir.3. human.

Organism-specific databases

CTDi79691.
GeneCardsiGC03P113724.
HGNCiHGNC:25771. QTRTD1.
HPAiHPA036827.
HPA036828.
neXtProtiNX_Q9H974.
PharmGKBiPA134925851.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0343.
GeneTreeiENSGT00530000063679.
HOGENOMiHOG000243329.
HOVERGENiHBG057274.
InParanoidiQ9H974.
KOiK15407.
OMAiMHNFEHY.
PhylomeDBiQ9H974.
TreeFamiTF317105.

Enzyme and pathway databases

UniPathwayiUPA00392.

Miscellaneous databases

ChiTaRSiQTRTD1. human.
GenomeRNAii79691.
NextBioi35535501.
PROiQ9H974.

Gene expression databases

BgeeiQ9H974.
CleanExiHS_QTRTD1.
ExpressionAtlasiQ9H974. baseline and differential.
GenevestigatoriQ9H974.

Family and domain databases

Gene3Di3.20.20.105. 2 hits.
HAMAPiMF_03043. QTRD1.
InterProiIPR028592. QTRTD1.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 2 hits.
TIGRFAMsiTIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Tissue: Tongue.
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Tissue: Lymph node.
  4. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph and Testis.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiQTRD1_HUMAN
AccessioniPrimary (citable) accession number: Q9H974
Secondary accession number(s): A6NGE9
, B7Z472, B7Z5R2, J3KR78, Q6IA59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: March 1, 2001
Last modified: March 4, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.