Q9H974 (QTRD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Queuine tRNA-ribosyltransferase subunit QTRTD1
EC=2.4.2.29

Alternative name(s):
Queuine tRNA-ribosyltransferase domain-containing protein 1

Gene names

Name:

QTRTD1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; tRNA-queuosine biosynthesis.
Subunit structure	Interacts with QTRT1 By similarity.
Subcellular location	Cytoplasm By similarity. Mitochondrion By similarity. Note: May associate with the mitochondrion outer membrane By similarity.
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Cellular component	Cytoplasm Mitochondrion
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Queuine tRNA-ribosyltransferase subunit QTRTD1		PRO_0000295633
Sites
	Active site	272	1	Nucleophile By similarity
	Metal binding	351	1	Zinc By similarity
	Metal binding	353	1	Zinc By similarity
	Metal binding	356	1	Zinc By similarity
	Metal binding	382	1	Zinc By similarity
Experimental info
	Sequence conflict	246	1	D → G in CAG33577. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9H974 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C9447FD900DD667B	FASTA	415	46,713
	10 20 30 40 50 60 MKLSLTKVVN GCRLGKIKNL GKTGDHTMDI PGCLLYTKTG SAPHLTHHTL HNIHGVPAMA 70 80 90 100 110 120 QLTLSSLAEH HEVLTEYKEG VGKFIGMPES LLYCSLHDPV SPCPAGYVTN KSVSVWSVAG 130 140 150 160 170 180 RVEMTVSKFM AIQKALQPDW FQCLSDGEVS CKEATSIKRV RKSVDRSLLF LDNCLRLQEE 190 200 210 220 230 240 SEVLQKSVII GVIEGGDVME ERLRSARETA KRPVGGFLLD GFQGNPTTLE ARLRLLSSVT 250 260 270 280 290 300 AELPEDKPRL ISGVSRPDEV LECIERGVDL FESFFPYQVT ERGCALTFSF DYQPNPEETL 310 320 330 340 350 360 LQQNGTQEEI KCMDQIKKIE TTGCNQEITS FEINLKEKKY QEDFNPLVRG CSCYCCKNHT 370 380 390 400 410 RAYIHHLLVT NELLAGVLLM MHNFEHYFGF FHYIREALKS DKLAQLKELI HRQAS

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed: 16641997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph and Testis.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK023022 mRNA. Translation: BAB14361.1. CR457296 mRNA. Translation: CAG33577.1. AC092896 Genomic DNA. No translation available. CH471052 Genomic DNA. Translation: EAW79613.1. BC034559 mRNA. Translation: AAH34559.1. BC039265 mRNA. Translation: AAH39265.1.
IPI	IPI00783033.
RefSeq	NP_078914.1. NM_024638.2.
UniGene	Hs.477162.
3D structure databases
HSSP	HSSP built from PDB template 1PXG based on UniProtKB P28720.
ProteinModelPortal	Q9H974.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H974. 3 interactions.
STRING	Q9H974.
PTM databases
PhosphoSite	Q9H974.
Polymorphism databases
DMDM	74752736.
Proteomic databases
PRIDE	Q9H974.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000281273; ENSP00000281273; ENSG00000151576. ENST00000485050; ENSP00000420682; ENSG00000151576.
GeneID	79691.
KEGG	hsa:79691.
UCSC	uc003eay.1. human.
Organism-specific databases
CTD	79691.
GeneCards	GC03P113724.
HGNC	HGNC:25771. QTRTD1.
HPA	HPA036828.
neXtProt	NX_Q9H974.
PharmGKB	PA134925851.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04755.
GeneTree	ENSGT00530000063679.
HOGENOM	HBG282507.
HOVERGEN	HBG057274.
InParanoid	Q9H974.
OMA	MHNFEHY.
PhylomeDB	Q9H974.
Gene expression databases
ArrayExpress	Q9H974.
Bgee	Q9H974.
CleanEx	HS_QTRTD1.
Genevestigator	Q9H974.
Family and domain databases
InterPro	IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 2 hits.
KO	K15407.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
SUPFAM	SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMs	TIGR00449. Tgt_general. 1 hit.
ProtoNet	Search...
Other
NextBio	68964.

Entry information

Entry name

QTRD1_HUMAN

Accession

Primary (citable) accession number: Q9H974
Secondary accession number(s): A6NGE9, Q6IA59

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.