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Protein

Queuine tRNA-ribosyltransferase accessory subunit 2

Gene

QTRT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi351ZincUniRule annotation1
Metal bindingi353ZincUniRule annotation1
Metal bindingi356ZincUniRule annotation1
Metal bindingi382Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • queuine tRNA-ribosyltransferase activity Source: UniProtKB

GO - Biological processi

  • queuosine biosynthetic process Source: UniProtKB
  • tRNA modification Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS07747-MONOMER.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferase accessory subunit 2UniRule annotation
Alternative name(s):
Queuine tRNA-ribosyltransferase domain-containing protein 1UniRule annotation
Gene namesi
Name:QTRT2UniRule annotation
Synonyms:QTRTD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:25771. QTRT2.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Mitochondrion outer membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

  • Note: May associate with the mitochondrion outer membrane.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000151576.
PharmGKBiPA134925851.

Polymorphism and mutation databases

BioMutaiQTRTD1.
DMDMi74752736.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002956331 – 415Queuine tRNA-ribosyltransferase accessory subunit 2Add BLAST415

Proteomic databases

EPDiQ9H974.
MaxQBiQ9H974.
PaxDbiQ9H974.
PeptideAtlasiQ9H974.
PRIDEiQ9H974.

PTM databases

iPTMnetiQ9H974.
PhosphoSitePlusiQ9H974.

Expressioni

Gene expression databases

BgeeiENSG00000151576.
CleanExiHS_QTRTD1.
ExpressionAtlasiQ9H974. baseline and differential.
GenevisibleiQ9H974. HS.

Organism-specific databases

HPAiHPA036827.
HPA036828.

Interactioni

Subunit structurei

Heterodimer of a catalytic subunit QTRT1 and an accessory subunit QTRT2.UniRule annotation1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi122812. 4 interactors.
IntActiQ9H974. 3 interactors.
STRINGi9606.ENSP00000281273.

Structurei

3D structure databases

ProteinModelPortaliQ9H974.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family. QTRT2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3909. Eukaryota.
COG0343. LUCA.
GeneTreeiENSGT00530000063679.
HOGENOMiHOG000243329.
HOVERGENiHBG057274.
InParanoidiQ9H974.
KOiK15407.
OMAiGCRLGKI.
OrthoDBiEOG091G099D.
PhylomeDBiQ9H974.
TreeFamiTF317105.

Family and domain databases

Gene3Di3.20.20.105. 2 hits.
HAMAPiMF_03043. QTRT2. 1 hit.
InterProiIPR028592. QTRTD1.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 2 hits.
TIGRFAMsiTIGR00449. tgt_general. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H974-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLSLTKVVN GCRLGKIKNL GKTGDHTMDI PGCLLYTKTG SAPHLTHHTL
60 70 80 90 100
HNIHGVPAMA QLTLSSLAEH HEVLTEYKEG VGKFIGMPES LLYCSLHDPV
110 120 130 140 150
SPCPAGYVTN KSVSVWSVAG RVEMTVSKFM AIQKALQPDW FQCLSDGEVS
160 170 180 190 200
CKEATSIKRV RKSVDRSLLF LDNCLRLQEE SEVLQKSVII GVIEGGDVME
210 220 230 240 250
ERLRSARETA KRPVGGFLLD GFQGNPTTLE ARLRLLSSVT AELPEDKPRL
260 270 280 290 300
ISGVSRPDEV LECIERGVDL FESFFPYQVT ERGCALTFSF DYQPNPEETL
310 320 330 340 350
LQQNGTQEEI KCMDQIKKIE TTGCNQEITS FEINLKEKKY QEDFNPLVRG
360 370 380 390 400
CSCYCCKNHT RAYIHHLLVT NELLAGVLLM MHNFEHYFGF FHYIREALKS
410
DKLAQLKELI HRQAS
Length:415
Mass (Da):46,713
Last modified:March 1, 2001 - v1
Checksum:iC9447FD900DD667B
GO
Isoform 2 (identifier: Q9H974-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Show »
Length:292
Mass (Da):33,471
Checksum:iDCFC6BF2B6F7C601
GO
Isoform 3 (identifier: Q9H974-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: MKLSLTKVVN...PCPAGYVTNK → MVCWQ

Show »
Length:309
Mass (Da):35,377
Checksum:iA2A2F97FE37E120D
GO
Isoform 4 (identifier: Q9H974-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVCWQDLEESLRM

Note: No experimental confirmation available.
Show »
Length:427
Mass (Da):48,203
Checksum:i3D4B55C405EDDAD5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti246D → G in CAG33577 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0451401 – 123Missing in isoform 2. 1 PublicationAdd BLAST123
Alternative sequenceiVSP_0451411 – 111MKLSL…YVTNK → MVCWQ in isoform 3. 1 PublicationAdd BLAST111
Alternative sequenceiVSP_0468591M → MVCWQDLEESLRM in isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023022 mRNA. Translation: BAB14361.1.
AK296938 mRNA. Translation: BAH12458.1.
AK299313 mRNA. Translation: BAH12998.1.
CR457296 mRNA. Translation: CAG33577.1.
AL833709 mRNA. No translation available.
AC092896 Genomic DNA. No translation available.
AC128687 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79613.1.
BC034559 mRNA. Translation: AAH34559.1.
BC039265 mRNA. Translation: AAH39265.1.
CCDSiCCDS33828.1. [Q9H974-1]
CCDS58844.1. [Q9H974-4]
CCDS58845.1. [Q9H974-3]
CCDS58846.1. [Q9H974-2]
RefSeqiNP_001243764.1. NM_001256835.1. [Q9H974-4]
NP_001243765.1. NM_001256836.1. [Q9H974-3]
NP_001243766.1. NM_001256837.1. [Q9H974-2]
NP_078914.1. NM_024638.3. [Q9H974-1]
XP_005247827.1. XM_005247770.3. [Q9H974-2]
XP_016862671.1. XM_017007182.1. [Q9H974-2]
XP_016862672.1. XM_017007183.1. [Q9H974-2]
UniGeneiHs.477162.

Genome annotation databases

EnsembliENST00000281273; ENSP00000281273; ENSG00000151576. [Q9H974-1]
ENST00000479882; ENSP00000418056; ENSG00000151576. [Q9H974-2]
ENST00000485050; ENSP00000420682; ENSG00000151576. [Q9H974-4]
ENST00000493014; ENSP00000419169; ENSG00000151576. [Q9H974-3]
GeneIDi79691.
KEGGihsa:79691.
UCSCiuc003eay.5. human. [Q9H974-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023022 mRNA. Translation: BAB14361.1.
AK296938 mRNA. Translation: BAH12458.1.
AK299313 mRNA. Translation: BAH12998.1.
CR457296 mRNA. Translation: CAG33577.1.
AL833709 mRNA. No translation available.
AC092896 Genomic DNA. No translation available.
AC128687 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79613.1.
BC034559 mRNA. Translation: AAH34559.1.
BC039265 mRNA. Translation: AAH39265.1.
CCDSiCCDS33828.1. [Q9H974-1]
CCDS58844.1. [Q9H974-4]
CCDS58845.1. [Q9H974-3]
CCDS58846.1. [Q9H974-2]
RefSeqiNP_001243764.1. NM_001256835.1. [Q9H974-4]
NP_001243765.1. NM_001256836.1. [Q9H974-3]
NP_001243766.1. NM_001256837.1. [Q9H974-2]
NP_078914.1. NM_024638.3. [Q9H974-1]
XP_005247827.1. XM_005247770.3. [Q9H974-2]
XP_016862671.1. XM_017007182.1. [Q9H974-2]
XP_016862672.1. XM_017007183.1. [Q9H974-2]
UniGeneiHs.477162.

3D structure databases

ProteinModelPortaliQ9H974.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122812. 4 interactors.
IntActiQ9H974. 3 interactors.
STRINGi9606.ENSP00000281273.

PTM databases

iPTMnetiQ9H974.
PhosphoSitePlusiQ9H974.

Polymorphism and mutation databases

BioMutaiQTRTD1.
DMDMi74752736.

Proteomic databases

EPDiQ9H974.
MaxQBiQ9H974.
PaxDbiQ9H974.
PeptideAtlasiQ9H974.
PRIDEiQ9H974.

Protocols and materials databases

DNASUi79691.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000281273; ENSP00000281273; ENSG00000151576. [Q9H974-1]
ENST00000479882; ENSP00000418056; ENSG00000151576. [Q9H974-2]
ENST00000485050; ENSP00000420682; ENSG00000151576. [Q9H974-4]
ENST00000493014; ENSP00000419169; ENSG00000151576. [Q9H974-3]
GeneIDi79691.
KEGGihsa:79691.
UCSCiuc003eay.5. human. [Q9H974-1]

Organism-specific databases

CTDi79691.
GeneCardsiQTRTD1.
HGNCiHGNC:25771. QTRT2.
HPAiHPA036827.
HPA036828.
neXtProtiNX_Q9H974.
OpenTargetsiENSG00000151576.
PharmGKBiPA134925851.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3909. Eukaryota.
COG0343. LUCA.
GeneTreeiENSGT00530000063679.
HOGENOMiHOG000243329.
HOVERGENiHBG057274.
InParanoidiQ9H974.
KOiK15407.
OMAiGCRLGKI.
OrthoDBiEOG091G099D.
PhylomeDBiQ9H974.
TreeFamiTF317105.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciZFISH:HS07747-MONOMER.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.

Miscellaneous databases

ChiTaRSiQTRTD1. human.
GenomeRNAii79691.
PROiQ9H974.

Gene expression databases

BgeeiENSG00000151576.
CleanExiHS_QTRTD1.
ExpressionAtlasiQ9H974. baseline and differential.
GenevisibleiQ9H974. HS.

Family and domain databases

Gene3Di3.20.20.105. 2 hits.
HAMAPiMF_03043. QTRT2. 1 hit.
InterProiIPR028592. QTRTD1.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 2 hits.
TIGRFAMsiTIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQTRT2_HUMAN
AccessioniPrimary (citable) accession number: Q9H974
Secondary accession number(s): A6NGE9
, B7Z472, B7Z5R2, J3KR78, Q6IA59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.