Q9H974 (QTRD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Queuine tRNA-ribosyltransferase subunit QTRTD1
EC=2.4.2.29

Alternative name(s):
Queuine tRNA-ribosyltransferase domain-containing protein 1

Gene names

Name:

QTRTD1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_03043
Catalytic activity	Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_03043
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_03043
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_03043
Subunit structure	Interacts with QTRT1 By similarity. HAMAP-Rule MF_03043
Subcellular location	Cytoplasm By similarity. Mitochondrion By similarity. Note: May associate with the mitochondrion outer membrane By similarity. HAMAP-Rule MF_03043
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H974-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H974-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Isoform 3 (identifier: Q9H974-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: MKLSLTKVVN...PCPAGYVTNK → MVCWQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Queuine tRNA-ribosyltransferase subunit QTRTD1 HAMAP-Rule MF_03043		PRO_0000295633
Sites
	Active site	272	1	Nucleophile By similarity
	Metal binding	351	1	Zinc By similarity
	Metal binding	353	1	Zinc By similarity
	Metal binding	356	1	Zinc By similarity
	Metal binding	382	1	Zinc By similarity
Natural variations
	Alternative sequence	1 – 123	123	Missing in isoform 2.		VSP_045140
	Alternative sequence	1 – 111	111	MKLSL…YVTNK → MVCWQ in isoform 3.		VSP_045141
Experimental info
	Sequence conflict	246	1	D → G in CAG33577. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C9447FD900DD667B	FASTA	415	46,713
	10 20 30 40 50 60 MKLSLTKVVN GCRLGKIKNL GKTGDHTMDI PGCLLYTKTG SAPHLTHHTL HNIHGVPAMA 70 80 90 100 110 120 QLTLSSLAEH HEVLTEYKEG VGKFIGMPES LLYCSLHDPV SPCPAGYVTN KSVSVWSVAG 130 140 150 160 170 180 RVEMTVSKFM AIQKALQPDW FQCLSDGEVS CKEATSIKRV RKSVDRSLLF LDNCLRLQEE 190 200 210 220 230 240 SEVLQKSVII GVIEGGDVME ERLRSARETA KRPVGGFLLD GFQGNPTTLE ARLRLLSSVT 250 260 270 280 290 300 AELPEDKPRL ISGVSRPDEV LECIERGVDL FESFFPYQVT ERGCALTFSF DYQPNPEETL 310 320 330 340 350 360 LQQNGTQEEI KCMDQIKKIE TTGCNQEITS FEINLKEKKY QEDFNPLVRG CSCYCCKNHT 370 380 390 400 410 RAYIHHLLVT NELLAGVLLM MHNFEHYFGF FHYIREALKS DKLAQLKELI HRQAS
	Isoform 2 [UniParc]. Checksum: DCFC6BF2B6F7C601	FASTA	292	33,471
	10 20 30 40 50 60 MTVSKFMAIQ KALQPDWFQC LSDGEVSCKE ATSIKRVRKS VDRSLLFLDN CLRLQEESEV 70 80 90 100 110 120 LQKSVIIGVI EGGDVMEERL RSARETAKRP VGGFLLDGFQ GNPTTLEARL RLLSSVTAEL 130 140 150 160 170 180 PEDKPRLISG VSRPDEVLEC IERGVDLFES FFPYQVTERG CALTFSFDYQ PNPEETLLQQ 190 200 210 220 230 240 NGTQEEIKCM DQIKKIETTG CNQEITSFEI NLKEKKYQED FNPLVRGCSC YCCKNHTRAY 250 260 270 280 290 IHHLLVTNEL LAGVLLMMHN FEHYFGFFHY IREALKSDKL AQLKELIHRQ AS
	Isoform 3 [UniParc]. Checksum: A2A2F97FE37E120D	FASTA	309	35,377
	10 20 30 40 50 60 MVCWQSVSVW SVAGRVEMTV SKFMAIQKAL QPDWFQCLSD GEVSCKEATS IKRVRKSVDR 70 80 90 100 110 120 SLLFLDNCLR LQEESEVLQK SVIIGVIEGG DVMEERLRSA RETAKRPVGG FLLDGFQGNP 130 140 150 160 170 180 TTLEARLRLL SSVTAELPED KPRLISGVSR PDEVLECIER GVDLFESFFP YQVTERGCAL 190 200 210 220 230 240 TFSFDYQPNP EETLLQQNGT QEEIKCMDQI KKIETTGCNQ EITSFEINLK EKKYQEDFNP 250 260 270 280 290 300 LVRGCSCYCC KNHTRAYIHH LLVTNELLAG VLLMMHNFEH YFGFFHYIRE ALKSDKLAQL KELIHRQAS

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Tongue.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymph and Testis.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK023022 mRNA. Translation: BAB14361.1. AK296938 mRNA. Translation: BAH12458.1. AK299313 mRNA. Translation: BAH12998.1. CR457296 mRNA. Translation: CAG33577.1. AC092896 Genomic DNA. No translation available. AC128687 Genomic DNA. No translation available. CH471052 Genomic DNA. Translation: EAW79613.1. BC034559 mRNA. Translation: AAH34559.1. BC039265 mRNA. Translation: AAH39265.1.
IPI	IPI00783033.
RefSeq	NP_001243764.1. NM_001256835.1. NP_001243765.1. NM_001256836.1. NP_001243766.1. NM_001256837.1. NP_078914.1. NM_024638.3.
UniGene	Hs.477162.
3D structure databases
ProteinModelPortal	Q9H974.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H974. 3 interactions.
STRING	9606.ENSP00000281273.
PTM databases
PhosphoSite	Q9H974.
Polymorphism databases
DMDM	74752736.
Proteomic databases
PaxDb	Q9H974.
PRIDE	Q9H974.
Protocols and materials databases
DNASU	79691.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000281273; ENSP00000281273; ENSG00000151576. ENST00000479882; ENSP00000418056; ENSG00000151576. ENST00000493014; ENSP00000419169; ENSG00000151576.
GeneID	79691.
KEGG	hsa:79691.
UCSC	uc003eay.3. human. uc011bir.2. human.
Organism-specific databases
CTD	79691.
GeneCards	GC03P113724.
HGNC	HGNC:25771. QTRTD1.
HPA	HPA036828.
neXtProt	NX_Q9H974.
PharmGKB	PA134925851.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0343.
HOGENOM	HOG000243329.
HOVERGEN	HBG057274.
InParanoid	Q9H974.
KO	K15407.
Enzyme and pathway databases
UniPathway	UPA00392.
Gene expression databases
ArrayExpress	Q9H974.
Bgee	Q9H974.
CleanEx	HS_QTRTD1.
Genevestigator	Q9H974.
Family and domain databases
Gene3D	3.20.20.105. 2 hits.
HAMAP	MF_03043. QTRD1.
InterPro	IPR002616. tRNA_ribo_trans-like. [Graphical view]
PANTHER	PTHR11962. PTHR11962. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
SUPFAM	SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMs	TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	79691.
NextBio	68964.

Entry information

Entry name

QTRD1_HUMAN

Accession

Primary (citable) accession number: Q9H974
Secondary accession number(s): A6NGE9 , B7Z472, B7Z5R2, Q6IA59

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.