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Q9H974 (QTRD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase subunit QTRTD1

EC=2.4.2.29
Alternative name(s):
Queuine tRNA-ribosyltransferase domain-containing protein 1
Gene names
Name:QTRTD1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_03043

Catalytic activity

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_03043

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_03043

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_03043

Subunit structure

Interacts with QTRT1 By similarity. HAMAP-Rule MF_03043

Subcellular location

Cytoplasm By similarity. Mitochondrion By similarity. Note: May associate with the mitochondrion outer membrane By similarity. HAMAP-Rule MF_03043

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   Cellular componentCytoplasm
Mitochondrion
   Coding sequence diversityAlternative splicing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrion

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionqueuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H974-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H974-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.
Isoform 3 (identifier: Q9H974-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: MKLSLTKVVN...PCPAGYVTNK → MVCWQ
Isoform 4 (identifier: Q9H974-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVCWQDLEESLRM
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Queuine tRNA-ribosyltransferase subunit QTRTD1 HAMAP-Rule MF_03043
PRO_0000295633

Sites

Active site2721Nucleophile By similarity
Metal binding3511Zinc By similarity
Metal binding3531Zinc By similarity
Metal binding3561Zinc By similarity
Metal binding3821Zinc By similarity

Natural variations

Alternative sequence1 – 123123Missing in isoform 2.
VSP_045140
Alternative sequence1 – 111111MKLSL…YVTNK → MVCWQ in isoform 3.
VSP_045141
Alternative sequence11M → MVCWQDLEESLRM in isoform 4.
VSP_046859

Experimental info

Sequence conflict2461D → G in CAG33577. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: C9447FD900DD667B

FASTA41546,713
        10         20         30         40         50         60 
MKLSLTKVVN GCRLGKIKNL GKTGDHTMDI PGCLLYTKTG SAPHLTHHTL HNIHGVPAMA 

        70         80         90        100        110        120 
QLTLSSLAEH HEVLTEYKEG VGKFIGMPES LLYCSLHDPV SPCPAGYVTN KSVSVWSVAG 

       130        140        150        160        170        180 
RVEMTVSKFM AIQKALQPDW FQCLSDGEVS CKEATSIKRV RKSVDRSLLF LDNCLRLQEE 

       190        200        210        220        230        240 
SEVLQKSVII GVIEGGDVME ERLRSARETA KRPVGGFLLD GFQGNPTTLE ARLRLLSSVT 

       250        260        270        280        290        300 
AELPEDKPRL ISGVSRPDEV LECIERGVDL FESFFPYQVT ERGCALTFSF DYQPNPEETL 

       310        320        330        340        350        360 
LQQNGTQEEI KCMDQIKKIE TTGCNQEITS FEINLKEKKY QEDFNPLVRG CSCYCCKNHT 

       370        380        390        400        410 
RAYIHHLLVT NELLAGVLLM MHNFEHYFGF FHYIREALKS DKLAQLKELI HRQAS 

« Hide

Isoform 2 [UniParc].

Checksum: DCFC6BF2B6F7C601
Show »

FASTA29233,471
Isoform 3 [UniParc].

Checksum: A2A2F97FE37E120D
Show »

FASTA30935,377
Isoform 4 [UniParc].

Checksum: 3D4B55C405EDDAD5
Show »

FASTA42748,203

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Tongue.
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Lymph node.
[4]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lymph and Testis.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK023022 mRNA. Translation: BAB14361.1.
AK296938 mRNA. Translation: BAH12458.1.
AK299313 mRNA. Translation: BAH12998.1.
CR457296 mRNA. Translation: CAG33577.1.
AL833709 mRNA. No translation available.
AC092896 Genomic DNA. No translation available.
AC128687 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79613.1.
BC034559 mRNA. Translation: AAH34559.1.
BC039265 mRNA. Translation: AAH39265.1.
RefSeqNP_001243764.1. NM_001256835.1.
NP_001243765.1. NM_001256836.1.
NP_001243766.1. NM_001256837.1.
NP_078914.1. NM_024638.3.
XP_005247827.1. XM_005247770.1.
UniGeneHs.477162.

3D structure databases

ProteinModelPortalQ9H974.
SMRQ9H974. Positions 1-414.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122812. 5 interactions.
IntActQ9H974. 3 interactions.
STRING9606.ENSP00000281273.

PTM databases

PhosphoSiteQ9H974.

Polymorphism databases

DMDM74752736.

Proteomic databases

PaxDbQ9H974.
PRIDEQ9H974.

Protocols and materials databases

DNASU79691.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000281273; ENSP00000281273; ENSG00000151576. [Q9H974-1]
ENST00000479882; ENSP00000418056; ENSG00000151576. [Q9H974-2]
ENST00000485050; ENSP00000420682; ENSG00000151576. [Q9H974-4]
ENST00000493014; ENSP00000419169; ENSG00000151576. [Q9H974-3]
GeneID79691.
KEGGhsa:79691.
UCSCuc003eay.4. human. [Q9H974-1]
uc011bir.3. human.

Organism-specific databases

CTD79691.
GeneCardsGC03P113724.
HGNCHGNC:25771. QTRTD1.
HPAHPA036827.
HPA036828.
neXtProtNX_Q9H974.
PharmGKBPA134925851.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000243329.
HOVERGENHBG057274.
InParanoidQ9H974.
KOK15407.
OMAMHNFEHY.
PhylomeDBQ9H974.
TreeFamTF317105.

Enzyme and pathway databases

UniPathwayUPA00392.

Gene expression databases

ArrayExpressQ9H974.
BgeeQ9H974.
CleanExHS_QTRTD1.
GenevestigatorQ9H974.

Family and domain databases

Gene3D3.20.20.105. 2 hits.
HAMAPMF_03043. QTRD1.
InterProIPR028592. QTRTD1.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. SSF51713. 2 hits.
TIGRFAMsTIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi79691.
NextBio35535501.
PROQ9H974.

Entry information

Entry nameQTRD1_HUMAN
AccessionPrimary (citable) accession number: Q9H974
Secondary accession number(s): A6NGE9 expand/collapse secondary AC list , B7Z472, B7Z5R2, J3KR78, Q6IA59
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM