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Q9H944

- MED20_HUMAN

UniProt

Q9H944 - MED20_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 20

Gene

MED20

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

    GO - Molecular functioni

    1. DNA-directed RNA polymerase activity Source: UniProtKB
    2. protein binding Source: IntAct
    3. RNA polymerase II transcription cofactor activity Source: InterPro

    GO - Biological processi

    1. gene expression Source: Reactome
    2. regulation of transcription from RNA polymerase II promoter Source: ProtInc
    3. transcription, DNA-templated Source: UniProtKB
    4. transcription from RNA polymerase II promoter Source: ProtInc
    5. transcription initiation from RNA polymerase II promoter Source: Reactome

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 20
    Alternative name(s):
    Mediator complex subunit 20
    TRF-proximal protein homolog
    Short name:
    hTRFP
    Gene namesi
    Name:MED20
    Synonyms:TRFP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:16840. MED20.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: UniProtKB
    2. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162395472.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 212212Mediator of RNA polymerase II transcription subunit 20PRO_0000065731Add
    BLAST

    Proteomic databases

    MaxQBiQ9H944.
    PaxDbiQ9H944.
    PeptideAtlasiQ9H944.
    PRIDEiQ9H944.

    PTM databases

    PhosphoSiteiQ9H944.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9H944.
    BgeeiQ9H944.
    CleanExiHS_MED20.
    GenevestigatoriQ9H944.

    Organism-specific databases

    HPAiHPA040717.

    Interactioni

    Subunit structurei

    Interacts with PPARG By similarity. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MED29Q9NX705EBI-394644,EBI-394656

    Protein-protein interaction databases

    BioGridi114862. 24 interactions.
    DIPiDIP-31449N.
    IntActiQ9H944. 11 interactions.
    MINTiMINT-275845.
    STRINGi9606.ENSP00000265350.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H944.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 20 family.Curated

    Phylogenomic databases

    eggNOGiNOG268717.
    HOGENOMiHOG000290707.
    HOVERGENiHBG106543.
    InParanoidiQ9H944.
    KOiK13528.
    OMAiKSHFQNA.
    OrthoDBiEOG7XSTFN.
    PhylomeDBiQ9H944.
    TreeFamiTF315156.

    Family and domain databases

    InterProiIPR013921. Mediator_Med20.
    [Graphical view]
    PfamiPF08612. Med20. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9H944-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA    50
    STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADTN FDVLMVKLKG 100
    FFQSAKASKI ETRGTRYQYC DFLVKVGTVT MGPSARGISV EVEYGPCVVA 150
    SDCWSLLLEF LQSFLGSHTP GAPAVFGNRH DAVYGPADTM VQYMELFNKI 200
    RKQQQVPVAG IR 212
    Length:212
    Mass (Da):23,222
    Last modified:March 1, 2001 - v1
    Checksum:i5AA7A39981EB1498
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti134 – 1363SAR → VP in AAD16169. (PubMed:9933582)Curated
    Sequence conflicti206 – 2072Missing in AAD16169. (PubMed:9933582)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF097725 mRNA. Translation: AAD16169.1.
    AK023092 mRNA. Translation: BAB14399.1.
    AL160163 Genomic DNA. Translation: CAI23487.1.
    BC012618 mRNA. Translation: AAH12618.1.
    BC019866 mRNA. Translation: AAH19866.1.
    BC032552 mRNA. Translation: AAH32552.1.
    BC040950 mRNA. Translation: AAH40950.1.
    AL050196 mRNA. Translation: CAB43314.1.
    CCDSiCCDS4862.1.
    PIRiT08801.
    RefSeqiNP_004266.2. NM_004275.3.
    UniGeneiHs.278434.

    Genome annotation databases

    EnsembliENST00000265350; ENSP00000265350; ENSG00000124641.
    GeneIDi9477.
    KEGGihsa:9477.
    UCSCiuc003orj.3. human.

    Polymorphism databases

    DMDMi29428258.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF097725 mRNA. Translation: AAD16169.1 .
    AK023092 mRNA. Translation: BAB14399.1 .
    AL160163 Genomic DNA. Translation: CAI23487.1 .
    BC012618 mRNA. Translation: AAH12618.1 .
    BC019866 mRNA. Translation: AAH19866.1 .
    BC032552 mRNA. Translation: AAH32552.1 .
    BC040950 mRNA. Translation: AAH40950.1 .
    AL050196 mRNA. Translation: CAB43314.1 .
    CCDSi CCDS4862.1.
    PIRi T08801.
    RefSeqi NP_004266.2. NM_004275.3.
    UniGenei Hs.278434.

    3D structure databases

    ProteinModelPortali Q9H944.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114862. 24 interactions.
    DIPi DIP-31449N.
    IntActi Q9H944. 11 interactions.
    MINTi MINT-275845.
    STRINGi 9606.ENSP00000265350.

    PTM databases

    PhosphoSitei Q9H944.

    Polymorphism databases

    DMDMi 29428258.

    Proteomic databases

    MaxQBi Q9H944.
    PaxDbi Q9H944.
    PeptideAtlasi Q9H944.
    PRIDEi Q9H944.

    Protocols and materials databases

    DNASUi 9477.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000265350 ; ENSP00000265350 ; ENSG00000124641 .
    GeneIDi 9477.
    KEGGi hsa:9477.
    UCSCi uc003orj.3. human.

    Organism-specific databases

    CTDi 9477.
    GeneCardsi GC06M041877.
    HGNCi HGNC:16840. MED20.
    HPAi HPA040717.
    MIMi 612915. gene.
    neXtProti NX_Q9H944.
    PharmGKBi PA162395472.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG268717.
    HOGENOMi HOG000290707.
    HOVERGENi HBG106543.
    InParanoidi Q9H944.
    KOi K13528.
    OMAi KSHFQNA.
    OrthoDBi EOG7XSTFN.
    PhylomeDBi Q9H944.
    TreeFami TF315156.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    ChiTaRSi MED20. human.
    GenomeRNAii 9477.
    NextBioi 35516.
    PROi Q9H944.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9H944.
    Bgeei Q9H944.
    CleanExi HS_MED20.
    Genevestigatori Q9H944.

    Family and domain databases

    InterProi IPR013921. Mediator_Med20.
    [Graphical view ]
    Pfami PF08612. Med20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex."
      Xiao H., Tao Y., Roeder R.G.
      J. Biol. Chem. 274:3937-3940(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 16-26; 36-51 AND 205-212, INTERACTION WITH RNA POLYMERASE II AND MED21.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver, Prostate, Testis and Uterus.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 120-212.
      Tissue: Uterus.
    6. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
      Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
      J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED18.
    7. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    8. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED1; MED18; MED21; MED28; MED29 AND MED31, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMED20_HUMAN
    AccessioniPrimary (citable) accession number: Q9H944
    Secondary accession number(s): O95821, Q5T8J4, Q9Y429
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3