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Protein

Mediator of RNA polymerase II transcription subunit 20

Gene

MED20

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 20
Alternative name(s):
Mediator complex subunit 20
TRF-proximal protein homolog
Short name:
hTRFP
Gene namesi
Name:MED20
Synonyms:TRFP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000124641.14
HGNCiHGNC:16840 MED20
MIMi612915 gene
neXtProtiNX_Q9H944

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000124641
PharmGKBiPA162395472

Polymorphism and mutation databases

BioMutaiMED20
DMDMi29428258

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657311 – 212Mediator of RNA polymerase II transcription subunit 20Add BLAST212

Proteomic databases

EPDiQ9H944
MaxQBiQ9H944
PaxDbiQ9H944
PeptideAtlasiQ9H944
PRIDEiQ9H944
ProteomicsDBi81283
TopDownProteomicsiQ9H944-1 [Q9H944-1]

PTM databases

iPTMnetiQ9H944
PhosphoSitePlusiQ9H944

Expressioni

Gene expression databases

BgeeiENSG00000124641
CleanExiHS_MED20
ExpressionAtlasiQ9H944 baseline and differential
GenevisibleiQ9H944 HS

Organism-specific databases

HPAiHPA040717

Interactioni

Subunit structurei

Interacts with PPARG (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.By similarity4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi114862, 131 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ9H944
DIPiDIP-31449N
IntActiQ9H944, 15 interactors
STRINGi9606.ENSP00000265350

Structurei

3D structure databases

ProteinModelPortaliQ9H944
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 20 family.Curated

Phylogenomic databases

eggNOGiKOG4309 Eukaryota
ENOG410XT23 LUCA
GeneTreeiENSGT00390000002060
HOGENOMiHOG000290707
HOVERGENiHBG106543
InParanoidiQ9H944
KOiK13528
OMAiQYLEHFT
OrthoDBiEOG091G0OWC
PhylomeDBiQ9H944
TreeFamiTF315156

Family and domain databases

InterProiView protein in InterPro
IPR013921 Mediator_Med20
PfamiView protein in Pfam
PF08612 Med20, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H944-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA
60 70 80 90 100
STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADTN FDVLMVKLKG
110 120 130 140 150
FFQSAKASKI ETRGTRYQYC DFLVKVGTVT MGPSARGISV EVEYGPCVVA
160 170 180 190 200
SDCWSLLLEF LQSFLGSHTP GAPAVFGNRH DAVYGPADTM VQYMELFNKI
210
RKQQQVPVAG IR
Length:212
Mass (Da):23,222
Last modified:March 1, 2001 - v1
Checksum:i5AA7A39981EB1498
GO
Isoform 2 (identifier: Q9H944-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-212: YQYCDFLVKV...QQQVPVAGIR → WSMAPVW

Note: No experimental confirmation available.
Show »
Length:123
Mass (Da):13,540
Checksum:iA2717B0D77BD7ACF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134 – 136SAR → VP in AAD16169 (PubMed:9933582).Curated3
Sequence conflicti206 – 207Missing in AAD16169 (PubMed:9933582).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057052117 – 212YQYCD…VAGIR → WSMAPVW in isoform 2. 1 PublicationAdd BLAST96

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097725 mRNA Translation: AAD16169.1
AK023092 mRNA Translation: BAB14399.1
AK293412 mRNA Translation: BAG56919.1
AL160163 Genomic DNA No translation available.
BC012618 mRNA Translation: AAH12618.1
BC019866 mRNA Translation: AAH19866.1
BC032552 mRNA Translation: AAH32552.1
BC040950 mRNA Translation: AAH40950.1
AL050196 mRNA Translation: CAB43314.1
CCDSiCCDS4862.1 [Q9H944-1]
CCDS83085.1 [Q9H944-2]
PIRiT08801
RefSeqiNP_001292384.1, NM_001305455.1
NP_001292385.1, NM_001305456.1
NP_001292386.1, NM_001305457.1 [Q9H944-2]
NP_004266.2, NM_004275.4 [Q9H944-1]
UniGeneiHs.278434

Genome annotation databases

EnsembliENST00000265350; ENSP00000265350; ENSG00000124641 [Q9H944-1]
ENST00000409312; ENSP00000386816; ENSG00000124641 [Q9H944-2]
GeneIDi9477
KEGGihsa:9477
UCSCiuc003ork.4 human [Q9H944-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMED20_HUMAN
AccessioniPrimary (citable) accession number: Q9H944
Secondary accession number(s): B4DE08
, O95821, Q5T8J4, Q9Y429
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 1, 2001
Last modified: June 20, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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