ID   PPIP2_HUMAN             Reviewed;         334 AA.
AC   Q9H939;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 4.
DT   24-JAN-2024, entry version 167.
DE   RecName: Full=Proline-serine-threonine phosphatase-interacting protein 2;
DE            Short=PEST phosphatase-interacting protein 2;
GN   Name=PSTPIP2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16177791; DOI=10.1038/nature03983;
RA   Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D.,
RA   Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA   Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J.,
RA   Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L.,
RA   Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A.,
RA   Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C.,
RA   Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA   Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA   Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT   "DNA sequence and analysis of human chromosome 18.";
RL   Nature 437:551-555(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 113-334 (ISOFORM 1).
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
CC   -!- FUNCTION: Binds to F-actin. May be involved in regulation of the actin
CC       cytoskeleton (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9H939-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9H939-2; Sequence=VSP_004070;
CC   -!- PTM: Phosphorylated on tyrosine. {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB14404.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC012569; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC090355; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC035395; AAH35395.1; -; mRNA.
DR   EMBL; AK023100; BAB14404.1; ALT_INIT; mRNA.
DR   CCDS; CCDS32820.2; -. [Q9H939-1]
DR   RefSeq; NP_077748.3; NM_024430.3. [Q9H939-1]
DR   AlphaFoldDB; Q9H939; -.
DR   SMR; Q9H939; -.
DR   BioGRID; 114512; 28.
DR   IntAct; Q9H939; 20.
DR   MINT; Q9H939; -.
DR   STRING; 9606.ENSP00000387261; -.
DR   GlyGen; Q9H939; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q9H939; -.
DR   PhosphoSitePlus; Q9H939; -.
DR   BioMuta; PSTPIP2; -.
DR   DMDM; 294862539; -.
DR   EPD; Q9H939; -.
DR   jPOST; Q9H939; -.
DR   MassIVE; Q9H939; -.
DR   MaxQB; Q9H939; -.
DR   PaxDb; 9606-ENSP00000387261; -.
DR   PeptideAtlas; Q9H939; -.
DR   ProteomicsDB; 81278; -. [Q9H939-1]
DR   ProteomicsDB; 81279; -. [Q9H939-2]
DR   Pumba; Q9H939; -.
DR   Antibodypedia; 22437; 111 antibodies from 21 providers.
DR   DNASU; 9050; -.
DR   Ensembl; ENST00000409746.5; ENSP00000387261.4; ENSG00000152229.18. [Q9H939-1]
DR   Ensembl; ENST00000589328.5; ENSP00000468622.1; ENSG00000152229.18. [Q9H939-2]
DR   GeneID; 9050; -.
DR   KEGG; hsa:9050; -.
DR   MANE-Select; ENST00000409746.5; ENSP00000387261.4; NM_024430.4; NP_077748.3.
DR   UCSC; uc002lbp.5; human. [Q9H939-1]
DR   AGR; HGNC:9581; -.
DR   CTD; 9050; -.
DR   DisGeNET; 9050; -.
DR   GeneCards; PSTPIP2; -.
DR   HGNC; HGNC:9581; PSTPIP2.
DR   HPA; ENSG00000152229; Group enriched (bone marrow, lymphoid tissue, skeletal muscle).
DR   MIM; 616046; gene.
DR   neXtProt; NX_Q9H939; -.
DR   OpenTargets; ENSG00000152229; -.
DR   PharmGKB; PA33932; -.
DR   VEuPathDB; HostDB:ENSG00000152229; -.
DR   eggNOG; KOG2398; Eukaryota.
DR   GeneTree; ENSGT00940000158788; -.
DR   HOGENOM; CLU_038196_1_1_1; -.
DR   InParanoid; Q9H939; -.
DR   OMA; EEADQNM; -.
DR   OrthoDB; 3740727at2759; -.
DR   PhylomeDB; Q9H939; -.
DR   TreeFam; TF313677; -.
DR   PathwayCommons; Q9H939; -.
DR   SignaLink; Q9H939; -.
DR   BioGRID-ORCS; 9050; 6 hits in 1159 CRISPR screens.
DR   ChiTaRS; PSTPIP2; human.
DR   GeneWiki; PSTPIP2; -.
DR   GenomeRNAi; 9050; -.
DR   Pharos; Q9H939; Tbio.
DR   PRO; PR:Q9H939; -.
DR   Proteomes; UP000005640; Chromosome 18.
DR   RNAct; Q9H939; Protein.
DR   Bgee; ENSG00000152229; Expressed in spleen and 127 other cell types or tissues.
DR   ExpressionAtlas; Q9H939; baseline and differential.
DR   GO; GO:0005884; C:actin filament; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR   GO; GO:0030041; P:actin filament polymerization; IBA:GO_Central.
DR   CDD; cd07672; F-BAR_PSTPIP2; 1.
DR   Gene3D; 1.20.1270.60; Arfaptin homology (AH) domain/BAR domain; 1.
DR   InterPro; IPR027267; AH/BAR_dom_sf.
DR   InterPro; IPR031160; F_BAR.
DR   InterPro; IPR001060; FCH_dom.
DR   InterPro; IPR042694; PSTPIP2_F-BAR.
DR   PANTHER; PTHR23065; PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1; 1.
DR   PANTHER; PTHR23065:SF9; PROLINE-SERINE-THREONINE PHOSPHATASE-INTERACTING PROTEIN 2; 1.
DR   Pfam; PF00611; FCH; 1.
DR   SMART; SM00055; FCH; 1.
DR   SUPFAM; SSF103657; BAR/IMD domain-like; 1.
DR   PROSITE; PS51741; F_BAR; 1.
DR   Genevisible; Q9H939; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Coiled coil; Cytoplasm; Membrane; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..334
FT                   /note="Proline-serine-threonine phosphatase-interacting
FT                   protein 2"
FT                   /id="PRO_0000058541"
FT   DOMAIN          4..264
FT                   /note="F-BAR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01077"
FT   REGION          295..322
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          66..166
FT                   /evidence="ECO:0000255"
FT   MOD_RES         323
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q99M15"
FT   MOD_RES         329
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q99M15"
FT   VAR_SEQ         248..334
FT                   /note="MYEQVRKSLEMCSIQRDIEYFVNQRKTGQIPPAPIMYENFYSSQKNAVPAGK
FT                   ATGPNLARRGPLPIPKSSPDDPNYSLVDDYSLLYQ -> HPSCMRISTPPRRMQSQQER
FT                   LQGLTWQGEDPSQFLKAHQMIPITLWLMTTVCSISKINETRAFSG (in isoform
FT                   2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_004070"
FT   VARIANT         322
FT                   /note="N -> D (in dbSNP:rs2276199)"
FT                   /id="VAR_059708"
FT   VARIANT         322
FT                   /note="N -> S (in dbSNP:rs16978507)"
FT                   /id="VAR_059709"
FT   CONFLICT        284
FT                   /note="Y -> H (in Ref. 3; BAB14404)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   334 AA;  38858 MW;  DD7DC17EC3F6E165 CRC64;
     MTRSLFKGNF WSADILSTIG YDNIIQHLNN GRKNCKEFED FLKERAAIEE RYGKDLLNLS
     RKKPCGQSEI NTLKRALEVF KQQVDNVAQC HIQLAQSLRE EARKMEEFRE KQKLQRKKTE
     LIMDAIHKQK SLQFKKTMDA KKNYEQKCRD KDEAEQAVSR SANLVNPKQQ EKLFVKLATS
     KTAVEDSDKA YMLHIGTLDK VREEWQSEHI KACEAFEAQE CERINFFRNA LWLHVNQLSQ
     QCVTSDEMYE QVRKSLEMCS IQRDIEYFVN QRKTGQIPPA PIMYENFYSS QKNAVPAGKA
     TGPNLARRGP LPIPKSSPDD PNYSLVDDYS LLYQ
//