Q9H8Y5 (ANKZ1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ankyrin repeat and zinc finger domain-containing protein 1

Alternative name(s):
Zinc finger protein 744

Gene names

Name:	ANKZF1
Synonyms:	ZNF744

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway By similarity.
Sequence similarities	Belongs to the ANKZF1/VMS1 family. Contains 2 ANK repeats. Contains 1 C2H2-type zinc finger.
Sequence caution	The sequence ABD65410.1 differs from that shown. Reason: Frameshift at position 318. The sequence BAA91596.1 differs from that shown. Reason: Frameshift at position 318.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	ANK repeat Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ORF	Q9Q2G4	2	EBI-724848,EBI-6248094	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	Ankyrin repeat and zinc finger domain-containing protein 1		PRO_0000247278
Regions
	Repeat	493 – 526	34	ANK 1
	Repeat	534 – 563	30	ANK 2
	Zinc finger	72 – 96	25	C2H2-type
	Coiled coil	609 – 659	51	Potential
Amino acid modifications
	Modified residue	258	1	Phosphoserine Ref.8
	Modified residue	398	1	Phosphoserine Ref.8 Ref.10
	Modified residue	533	1	Phosphoserine Ref.8
	Modified residue	607	1	Phosphothreonine Ref.8
	Modified residue	675	1	Phosphoserine Ref.7
Natural variations
	Natural variant	569	1	R → W. Corresponds to variant rs2293076 [ dbSNP | Ensembl ].		VAR_048269
	Natural variant	676	1	P → L. Corresponds to variant rs2293079 [ dbSNP | Ensembl ].		VAR_048270

Sequences

	Sequence		Length	Mass (Da)
	Q9H8Y5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 44F7930A530F1355	FASTA	726	80,927
	10 20 30 40 50 60 MSPAPDAAPA PASISLFDLS ADAPVFQGLS LVSHAPGEAL ARAPRTSCSG SGERESPERK 70 80 90 100 110 120 LLQGPMDISE KLFCSTCDQT FQNHQEQREH YKLDWHRFNL KQRLKDKPLL SALDFEKQSS 130 140 150 160 170 180 TGDLSSISGS EDSDSASEED LQTLDRERAT FEKLSRPPGF YPHRVLFQNA QGQFLYAYRC 190 200 210 220 230 240 VLGPHQDPPE EAELLLQNLQ SRGPRDCVVL MAAAGHFAGA IFQGREVVTH KTFHRYTVRA 250 260 270 280 290 300 KRGTAQGLRD ARGGPSHSAG ANLRRYNEAT LYKDVRDLLA GPSWAKALEE AGTILLRAPR 310 320 330 340 350 360 SGRSLFFGGK GAPLQRGDPR LWDIPLATRR PTFQELQRVL HKLTTLHVYE EDPREAVRLH 370 380 390 400 410 420 SPQTHWKTVR EERKKPTEEE IRKICRDEKE ALGQNEESPK QGSGSEGEDG FQVELELVEL 430 440 450 460 470 480 TVGTLDLCES EVLPKRRRRK RNKKEKSRDQ EAGAHRTLLQ QTQEEEPSTQ SSQAVAAPLG 490 500 510 520 530 540 PLLDEAKAPG QPELWNALLA ACRAGDVGVL KLQLAPSPAD PRVLSLLSAP LGSGGFTLLH 550 560 570 580 590 600 AAAAAGRGSV VRLLLEAGAD PTVQDSRARP PYTVAADKST RNEFRRFMEK NPDAYDYNKA 610 620 630 640 650 660 QVPGPLTPEM EARQATRKRE QKAARRQREE QQQRQQEQEE REREEQRRFA ALSDREKRAL 670 680 690 700 710 720 AAERRLAAQL GAPTSPIPDS AIVNTRRCWS CGASLQGLTP FHYLDFSFCS TRCLQDHRRQ AGRPSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Zhao Y., Li Y., Yuan W., Wu X., Liu M. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Eye.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-675, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258; SER-398; SER-533 AND THR-607, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-398, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ412564 mRNA. Translation: ABD65410.1. Frameshift. AK001277 mRNA. Translation: BAA91596.1. Frameshift. AK023206 mRNA. Translation: BAB14462.1. BC000238 mRNA. Translation: AAH00238.1. BC008948 mRNA. Translation: AAH08948.1.
IPI	IPI00335437.
RefSeq	NP_001035869.1. NM_001042410.1. NP_060559.2. NM_018089.2.
UniGene	Hs.437647.
3D structure databases
ProteinModelPortal	Q9H8Y5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H8Y5. 6 interactions.
MINT	MINT-1387900.
STRING	9606.ENSP00000321617.
PTM databases
PhosphoSite	Q9H8Y5.
Polymorphism databases
DMDM	74761542.
Proteomic databases
PaxDb	Q9H8Y5.
PeptideAtlas	Q9H8Y5.
PRIDE	Q9H8Y5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000323348; ENSP00000321617; ENSG00000163516. ENST00000410034; ENSP00000386337; ENSG00000163516.
GeneID	55139.
KEGG	hsa:55139.
UCSC	uc002vkg.3. human.
Organism-specific databases
CTD	55139.
GeneCards	GC02P220094.
HGNC	HGNC:25527. ANKZF1.
HPA	HPA035208.
neXtProt	NX_Q9H8Y5.
PharmGKB	PA143485305.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG323124.
HOGENOM	HOG000033947.
HOVERGEN	HBG080842.
InParanoid	Q9H8Y5.
OMA	DFSFCST.
OrthoDB	EOG4ZGPC9.
PhylomeDB	Q9H8Y5.
Gene expression databases
ArrayExpress	Q9H8Y5.
Bgee	Q9H8Y5.
CleanEx	HS_ANKZF1.
Genevestigator	Q9H8Y5.
GermOnline	ENSG00000163516. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF00023. Ank. 1 hit. [Graphical view]
SMART	SM00248. ANK. 2 hits. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ANKZF1. human.
GenomeRNAi	55139.
NextBio	58830.

Entry information

Entry name

ANKZ1_HUMAN

Accession

Primary (citable) accession number: Q9H8Y5
Secondary accession number(s): Q9NVZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.