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Protein

Inositol-pentakisphosphate 2-kinase

Gene

IPPK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis.2 Publications

Catalytic activityi

ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol hexakisphosphate.1 Publication

Kineticsi

  1. KM=0.43 µM for Ins(1,3,4,5,6)P51 Publication
  2. KM=21 µM for ATP1 Publication
  1. Vmax=31 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • inositol pentakisphosphate 2-kinase activity Source: HGNC

GO - Biological processi

  • inositol phosphate biosynthetic process Source: GO_Central
  • inositol phosphate metabolic process Source: Reactome
  • inositol phosphorylation Source: BHF-UCL
  • mitophagy in response to mitochondrial depolarization Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05071-MONOMER.
ReactomeiR-HSA-1855167. Synthesis of pyrophosphates in the cytosol.
R-HSA-1855191. Synthesis of IPs in the nucleus.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-pentakisphosphate 2-kinase (EC:2.7.1.158)
Alternative name(s):
IPK1 homolog
Inositol-1,3,4,5,6-pentakisphosphate 2-kinase
Ins(1,3,4,5,6)P5 2-kinase
Short name:
InsP5 2-kinase
Gene namesi
Name:IPPK
Synonyms:C9orf12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:14645. IPPK.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • intracellular Source: BHF-UCL
  • nucleoplasm Source: Reactome
  • nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25970.

Polymorphism and mutation databases

BioMutaiIPPK.
DMDMi74752731.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Inositol-pentakisphosphate 2-kinasePRO_0000110529Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei282 – 2821PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H8X2.
MaxQBiQ9H8X2.
PaxDbiQ9H8X2.
PeptideAtlasiQ9H8X2.
PRIDEiQ9H8X2.

PTM databases

iPTMnetiQ9H8X2.
PhosphoSiteiQ9H8X2.

Expressioni

Tissue specificityi

Ubiquitously expressed, with high expression in heart, brain, testis and placenta.1 Publication

Gene expression databases

BgeeiENSG00000127080.
CleanExiHS_IPPK.
ExpressionAtlasiQ9H8X2. baseline and differential.
GenevisibleiQ9H8X2. HS.

Organism-specific databases

HPAiHPA020603.

Interactioni

Protein-protein interaction databases

BioGridi122279. 98 interactions.
IntActiQ9H8X2. 1 interaction.
STRINGi9606.ENSP00000287996.

Structurei

3D structure databases

ProteinModelPortaliQ9H8X2.
SMRiQ9H8X2. Positions 7-219, 320-464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi136 – 1405EXKPK motif

Domaini

The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.

Sequence similaritiesi

Belongs to the IPK1 type 2 family.Curated

Phylogenomic databases

eggNOGiKOG4749. Eukaryota.
ENOG410XSF2. LUCA.
GeneTreeiENSGT00390000010053.
HOGENOMiHOG000113092.
HOVERGENiHBG079461.
InParanoidiQ9H8X2.
KOiK10572.
OMAiTVMSTRF.
OrthoDBiEOG091G05DL.
PhylomeDBiQ9H8X2.
TreeFamiTF106142.

Family and domain databases

InterProiIPR009286. Ins_P5_2-kin.
[Graphical view]
PfamiPF06090. Ins_P5_2-kin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H8X2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEGKMDENE WGYHGEGNKS LVVAHAQRCV VLRFLKFPPN RKKTSEEIFQ
60 70 80 90 100
HLQNIVDFGK NVMKEFLGEN YVHYGEVVQL PLEFVKQLCL KIQSERPESR
110 120 130 140 150
CDKDLDTLSG YAMCLPNLTR LQTYRFAEHR PILCVEIKPK CGFIPFSSDV
160 170 180 190 200
THEMKHKVCR YCMHQHLKVA TGKWKQISKY CPLDLYSGNK QRMHFALKSL
210 220 230 240 250
LQEAQNNLKI FKNGELIYGC KDARSPVADW SELAHHLKPF FFPSNGLASG
260 270 280 290 300
PHCTRAVIRE LVHVITRVLL SGSDKGRAGT LSPGLGPQGP RVCEASPFSR
310 320 330 340 350
SLRCQGKNTP ERSGLPKGCL LYKTLQVQML DLLDIEGLYP LYNRVERYLE
360 370 380 390 400
EFPEERKTLQ IDGPYDEAFY QKLLDLSTED DGTVAFALTK VQQYRVAMTA
410 420 430 440 450
KDCSIMIALS PCLQDASSDQ RPVVPSSRSR FAFSVSVLDL DLKPYESIPH
460 470 480 490
QYKLDGKIVN YYSKTVRAKD NAVMSTRFKE SEDCTLVLHK V
Length:491
Mass (Da):56,017
Last modified:March 1, 2001 - v1
Checksum:iAE7A125F3BD709C0
GO

Sequence cautioni

The sequence BAB14866 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAI12593 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti264 – 2641V → A in BAB14866 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti277 – 2771R → W.
Corresponds to variant rs2277168 [ dbSNP | Ensembl ].
VAR_049641
Natural varianti376 – 3761L → F.
Corresponds to variant rs2277170 [ dbSNP | Ensembl ].
VAR_049642

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF520811 mRNA. Translation: AAM75353.1.
AF351202 mRNA. Translation: AAK69692.1.
AK023225 mRNA. Translation: BAB14476.1.
AK024267 mRNA. Translation: BAB14866.1. Different initiation.
AL137074, AL157827 Genomic DNA. Translation: CAI14368.1.
AL157827, AL137074 Genomic DNA. Translation: CAI12586.1.
AL157827 Genomic DNA. Translation: CAI12593.1. Sequence problems.
BC026154 mRNA. Translation: AAH26154.1.
CCDSiCCDS6699.1.
RefSeqiNP_073592.1. NM_022755.5.
UniGeneiHs.459896.
Hs.713775.

Genome annotation databases

EnsembliENST00000287996; ENSP00000287996; ENSG00000127080.
GeneIDi64768.
KEGGihsa:64768.
UCSCiuc004asl.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF520811 mRNA. Translation: AAM75353.1.
AF351202 mRNA. Translation: AAK69692.1.
AK023225 mRNA. Translation: BAB14476.1.
AK024267 mRNA. Translation: BAB14866.1. Different initiation.
AL137074, AL157827 Genomic DNA. Translation: CAI14368.1.
AL157827, AL137074 Genomic DNA. Translation: CAI12586.1.
AL157827 Genomic DNA. Translation: CAI12593.1. Sequence problems.
BC026154 mRNA. Translation: AAH26154.1.
CCDSiCCDS6699.1.
RefSeqiNP_073592.1. NM_022755.5.
UniGeneiHs.459896.
Hs.713775.

3D structure databases

ProteinModelPortaliQ9H8X2.
SMRiQ9H8X2. Positions 7-219, 320-464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122279. 98 interactions.
IntActiQ9H8X2. 1 interaction.
STRINGi9606.ENSP00000287996.

PTM databases

iPTMnetiQ9H8X2.
PhosphoSiteiQ9H8X2.

Polymorphism and mutation databases

BioMutaiIPPK.
DMDMi74752731.

Proteomic databases

EPDiQ9H8X2.
MaxQBiQ9H8X2.
PaxDbiQ9H8X2.
PeptideAtlasiQ9H8X2.
PRIDEiQ9H8X2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000287996; ENSP00000287996; ENSG00000127080.
GeneIDi64768.
KEGGihsa:64768.
UCSCiuc004asl.1. human.

Organism-specific databases

CTDi64768.
GeneCardsiIPPK.
HGNCiHGNC:14645. IPPK.
HPAiHPA020603.
neXtProtiNX_Q9H8X2.
PharmGKBiPA25970.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4749. Eukaryota.
ENOG410XSF2. LUCA.
GeneTreeiENSGT00390000010053.
HOGENOMiHOG000113092.
HOVERGENiHBG079461.
InParanoidiQ9H8X2.
KOiK10572.
OMAiTVMSTRF.
OrthoDBiEOG091G05DL.
PhylomeDBiQ9H8X2.
TreeFamiTF106142.

Enzyme and pathway databases

BioCyciMetaCyc:HS05071-MONOMER.
ReactomeiR-HSA-1855167. Synthesis of pyrophosphates in the cytosol.
R-HSA-1855191. Synthesis of IPs in the nucleus.

Miscellaneous databases

ChiTaRSiIPPK. human.
GenomeRNAii64768.
PROiQ9H8X2.

Gene expression databases

BgeeiENSG00000127080.
CleanExiHS_IPPK.
ExpressionAtlasiQ9H8X2. baseline and differential.
GenevisibleiQ9H8X2. HS.

Family and domain databases

InterProiIPR009286. Ins_P5_2-kin.
[Graphical view]
PfamiPF06090. Ins_P5_2-kin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIPPK_HUMAN
AccessioniPrimary (citable) accession number: Q9H8X2
Secondary accession number(s): Q5T9F7, Q9H7V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.