Reviewed, UniProtKB/Swiss-Prot Q9H8W4 (PKHF2_HUMAN)

Names and origin

Protein names

Recommended name:
    Pleckstrin homology domain-containing family F member 2
      Short name=PH domain-containing family F member 2
Alternative name(s):
    PH and FYVE domain-containing protein 2
      Short name=Phafin-2
    Zinc finger FYVE domain-containing protein 18

Gene names

Name:	PLEKHF2
Synonyms:	ZFYVE18

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities

Contains 1 FYVE-type zinc finger. Contains 1 PH domain.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	transport vesicle Inferred from direct assay. Source: LIFEdb
Molecular function	protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CHIC2	Q9UKJ5	2	EBI-742388,EBI-741528
MBIP	Q9NS73	2	EBI-742388,EBI-741953
PRKAR1A	P10644	2	EBI-742388,EBI-476431
RABAC1	Q9UI14	2	EBI-742388,EBI-712367

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Pleckstrin homology domain-containing family F member 2		PRO_0000251600
Regions
	Domain	35 – 131	97	PH
	Zinc finger	152 – 212	61	FYVE-type
Amino acid modifications
	Modified residue	44	1	N6-acetyllysine Ref.8
	Modified residue	239	1	Phosphoserine Ref.5 Ref.6
	Modified residue	248	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9H8W4-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F5E3F84595A98886	FASTA	249	27,798
	10 20 30 40 50 60 MVDRLANSEA NTRRISIVEN CFGAAGQPLT IPGRVLIGEG VLTKLCRKKP KARQFFLFND 70 80 90 100 110 120 ILVYGNIVIQ KKKYNKQHII PLENVTIDSI KDEGDLRNGW LIKTPTKSFA VYAATATEKS 130 140 150 160 170 180 EWMNHINKCV TDLLSKSGKT PSNEHAAVWV PDSEATVCMR CQKAKFTPVN RRHHCRKCGF 190 200 210 220 230 240 VVCGPCSEKR FLLPSQSSKP VRICDFCYDL LSAGDMATCQ PARSDSYSQS LKSPLNDMSD DDDDDDSSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phafin 2, PH and FYVE domain-containing protein 2." Shi H., Hong W. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Melanoma.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-239, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-239 AND SER-248, MASS SPECTROMETRY.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-44, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF434819 mRNA. Translation: AAL30774.1. AK023249 mRNA. Translation: BAB14486.1. AL834473 mRNA. Translation: CAD39132.1. BC011806 mRNA. Translation: AAH11806.1.
IPI	IPI00002424.
RefSeq	NP_078889.1.
UniGene	Hs.29724
3D structure databases
HSSP	HSSP built from PDB template 1HYJ based on UniProtKB Q15075.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H8W4. 26 interactions.
PTM databases
PhosphoSite	Q9H8W4.
Proteomic databases
PRIDE	Q9H8W4.
Genome annotation databases
Ensembl	ENST00000315367; ENSP00000322373; ENSG00000175895; Homo sapiens. [Genome view]
GeneID	79666.
KEGG	hsa:79666.
UCSC	uc003yhn.1. human.
Organism-specific databases
CTD	79666.
GeneCards	GC08P096215.
HGNC	HGNC:20757. PLEKHF2.
PharmGKB	PA128394715.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9H8W4.
OMA	DEGELRN
OrthoDB	EOG93N9ZM
Gene expression databases
ArrayExpress	Q9H8W4.
Bgee	Q9H8W4.
CleanEx	HS_PLEKHF2.
Genevestigator	Q9H8W4.
GermOnline	ENSG00000175895. Homo sapiens.
Family and domain databases
InterPro	IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000306. Znf_FYVE. IPR017455. Znf_FYVE-rel. IPR011011. Znf_FYVE_PHD. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF01363. FYVE. 1 hit. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00064. FYVE. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50178. ZF_FYVE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	68872.

Entry information

Entry name

PKHF2_HUMAN

Accession

Primary (citable) accession number: Q9H8W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	March 1, 2001
Last modified:	November 24, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.