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Protein

AKT-interacting protein

Gene

AKTIP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade.2 Publications

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • early endosome to late endosome transport Source: UniProtKB
  • endosome organization Source: UniProtKB
  • endosome to lysosome transport Source: UniProtKB
  • lysosome organization Source: UniProtKB
  • positive regulation of protein binding Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • protein transport Source: UniProtKB-KW
  • protein ubiquitination Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Protein transport, Transport

Enzyme and pathway databases

SignaLinkiQ9H8T0.

Names & Taxonomyi

Protein namesi
Recommended name:
AKT-interacting protein
Alternative name(s):
Ft1
Fused toes protein homolog
Gene namesi
Name:AKTIP
Synonyms:FTS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:16710. AKTIP.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • FHF complex Source: UniProtKB
  • Golgi apparatus Source: HPA
  • nucleolus Source: HPA
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi106 – 1072WF → AA: Impairs interaction with HOOK1, HOOK2 and HOOK3. 1 Publication

Organism-specific databases

PharmGKBiPA162376210.

Polymorphism and mutation databases

BioMutaiAKTIP.
DMDMi54035954.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292AKT-interacting proteinPRO_0000082609Add
BLAST

Proteomic databases

MaxQBiQ9H8T0.
PaxDbiQ9H8T0.
PRIDEiQ9H8T0.

PTM databases

PhosphoSiteiQ9H8T0.

Expressioni

Gene expression databases

BgeeiQ9H8T0.
CleanExiHS_AKTIP.
ExpressionAtlasiQ9H8T0. baseline and differential.
GenevestigatoriQ9H8T0.

Organism-specific databases

HPAiHPA041794.
HPA046300.

Interactioni

Subunit structurei

Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with HOOK1, HOOK2 and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex). Also interacts with AKT1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOOK1Q9UJC33EBI-711399,EBI-746704

Protein-protein interaction databases

BioGridi122157. 34 interactions.
IntActiQ9H8T0. 29 interactions.
STRINGi9606.ENSP00000300245.

Structurei

3D structure databases

ProteinModelPortaliQ9H8T0.
SMRiQ9H8T0. Positions 117-223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. FTS subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG285076.
GeneTreeiENSGT00390000010125.
HOGENOMiHOG000033885.
HOVERGENiHBG095637.
InParanoidiQ9H8T0.
OMAiEDHLWQV.
OrthoDBiEOG7QG45C.
PhylomeDBiQ9H8T0.
TreeFamiTF314386.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H8T0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPFWSMSTS SVRKRSEGEE KTLTGDVKTS PPRTAPKKQL PSIPKNALPI
60 70 80 90 100
TKPTSPAPAA QSTNGTHASY GPFYLEYSLL AEFTLVVKQK LPGVYVQPSY
110 120 130 140 150
RSALMWFGVI FIRHGLYQDG VFKFTVYIPD NYPDGDCPRL VFDIPVFHPL
160 170 180 190 200
VDPTSGELDV KRAFAKWRRN HNHIWQVLMY ARRVFYKIDT ASPLNPEAAV
210 220 230 240 250
LYEKDIQLFK SKVVDSVKVC TARLFDQPKI EDPYAISFSP WNPSVHDEAR
260 270 280 290
EKMLTQKKPE EQHNKSVHVA GLSWVKPGSV QPFSKEEKTV AT
Length:292
Mass (Da):33,128
Last modified:March 1, 2001 - v1
Checksum:i3DD4E32980463324
GO
Isoform 2 (identifier: Q9H8T0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-256: Q → QK

Show »
Length:293
Mass (Da):33,256
Checksum:iCE781BABE3F9695C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti212 – 2121K → N in AAH95401 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei256 – 2561Q → QK in isoform 2. 2 PublicationsVSP_037631

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023320 mRNA. Translation: BAB14524.1.
CR457308 mRNA. Translation: CAG33589.1.
AK222743 mRNA. Translation: BAD96463.1.
CH471092 Genomic DNA. Translation: EAW82805.1.
BC001134 mRNA. Translation: AAH01134.1.
BC095401 mRNA. Translation: AAH95401.1.
CCDSiCCDS10749.1. [Q9H8T0-1]
RefSeqiNP_001012398.1. NM_001012398.1. [Q9H8T0-1]
NP_071921.1. NM_022476.2. [Q9H8T0-1]
XP_005256151.1. XM_005256094.3. [Q9H8T0-2]
XP_005256152.1. XM_005256095.3. [Q9H8T0-2]
XP_005256153.1. XM_005256096.3. [Q9H8T0-2]
XP_005256154.1. XM_005256097.3. [Q9H8T0-2]
XP_005256155.1. XM_005256098.3. [Q9H8T0-2]
UniGeneiHs.380897.

Genome annotation databases

EnsembliENST00000300245; ENSP00000300245; ENSG00000166971. [Q9H8T0-2]
ENST00000394657; ENSP00000378152; ENSG00000166971. [Q9H8T0-1]
ENST00000570004; ENSP00000455874; ENSG00000166971. [Q9H8T0-1]
GeneIDi64400.
KEGGihsa:64400.
UCSCiuc002ehk.3. human. [Q9H8T0-1]
uc002ehm.3. human. [Q9H8T0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023320 mRNA. Translation: BAB14524.1.
CR457308 mRNA. Translation: CAG33589.1.
AK222743 mRNA. Translation: BAD96463.1.
CH471092 Genomic DNA. Translation: EAW82805.1.
BC001134 mRNA. Translation: AAH01134.1.
BC095401 mRNA. Translation: AAH95401.1.
CCDSiCCDS10749.1. [Q9H8T0-1]
RefSeqiNP_001012398.1. NM_001012398.1. [Q9H8T0-1]
NP_071921.1. NM_022476.2. [Q9H8T0-1]
XP_005256151.1. XM_005256094.3. [Q9H8T0-2]
XP_005256152.1. XM_005256095.3. [Q9H8T0-2]
XP_005256153.1. XM_005256096.3. [Q9H8T0-2]
XP_005256154.1. XM_005256097.3. [Q9H8T0-2]
XP_005256155.1. XM_005256098.3. [Q9H8T0-2]
UniGeneiHs.380897.

3D structure databases

ProteinModelPortaliQ9H8T0.
SMRiQ9H8T0. Positions 117-223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122157. 34 interactions.
IntActiQ9H8T0. 29 interactions.
STRINGi9606.ENSP00000300245.

PTM databases

PhosphoSiteiQ9H8T0.

Polymorphism and mutation databases

BioMutaiAKTIP.
DMDMi54035954.

Proteomic databases

MaxQBiQ9H8T0.
PaxDbiQ9H8T0.
PRIDEiQ9H8T0.

Protocols and materials databases

DNASUi64400.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300245; ENSP00000300245; ENSG00000166971. [Q9H8T0-2]
ENST00000394657; ENSP00000378152; ENSG00000166971. [Q9H8T0-1]
ENST00000570004; ENSP00000455874; ENSG00000166971. [Q9H8T0-1]
GeneIDi64400.
KEGGihsa:64400.
UCSCiuc002ehk.3. human. [Q9H8T0-1]
uc002ehm.3. human. [Q9H8T0-2]

Organism-specific databases

CTDi64400.
GeneCardsiGC16M053524.
HGNCiHGNC:16710. AKTIP.
HPAiHPA041794.
HPA046300.
MIMi608483. gene.
neXtProtiNX_Q9H8T0.
PharmGKBiPA162376210.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG285076.
GeneTreeiENSGT00390000010125.
HOGENOMiHOG000033885.
HOVERGENiHBG095637.
InParanoidiQ9H8T0.
OMAiEDHLWQV.
OrthoDBiEOG7QG45C.
PhylomeDBiQ9H8T0.
TreeFamiTF314386.

Enzyme and pathway databases

SignaLinkiQ9H8T0.

Miscellaneous databases

GeneWikiiAKTIP.
GenomeRNAii64400.
NextBioi66346.
PROiQ9H8T0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H8T0.
CleanExiHS_AKTIP.
ExpressionAtlasiQ9H8T0. baseline and differential.
GenevestigatoriQ9H8T0.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Ovary.
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Dermoid cancer.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Skin.
  6. "Regulation of apoptosis by the Ft1 protein, a new modulator of protein kinase B/Akt."
    Remy I., Michnick S.W.
    Mol. Cell. Biol. 24:1493-1504(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH AKT1, SUBCELLULAR LOCATION.
  7. "An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex."
    Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W.
    Mol. Biol. Cell 19:5059-5071(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, ASSOCIATION WITH THE HOPS COMPLEX, MUTAGENESIS OF 106-TRP-PHE-107.

Entry informationi

Entry nameiAKTIP_HUMAN
AccessioniPrimary (citable) accession number: Q9H8T0
Secondary accession number(s): Q503B1, Q53H38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2001
Last modified: April 29, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Lacks the conserved Cys residue necessary for ubiquitin-conjugating enzyme E2 activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.