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Q9H8T0 (AKTIP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AKT-interacting protein
Alternative name(s):
Ft1
Fused toes protein homolog
Gene names
Name:AKTIP
Synonyms:FTS
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. Ref.6 Ref.7

Subunit structure

Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with HOOK1, HOOK2 and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex). Also interacts with AKT1. Ref.6 Ref.7

Subcellular location

Cytoplasm. Cell membrane; Peripheral membrane protein Ref.6.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family. FTS subfamily.

Caution

Lacks the conserved Cys residue necessary for ubiquitin-conjugating enzyme E2 activity.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H8T0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H8T0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-256: Q → QK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292AKT-interacting protein
PRO_0000082609

Natural variations

Alternative sequence2561Q → QK in isoform 2.
VSP_037631

Experimental info

Mutagenesis106 – 1072WF → AA: Impairs interaction with HOOK1, HOOK2 and HOOK3. Ref.7
Sequence conflict2121K → N in AAH95401. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 3DD4E32980463324

FASTA29233,128
        10         20         30         40         50         60 
MNPFWSMSTS SVRKRSEGEE KTLTGDVKTS PPRTAPKKQL PSIPKNALPI TKPTSPAPAA 

        70         80         90        100        110        120 
QSTNGTHASY GPFYLEYSLL AEFTLVVKQK LPGVYVQPSY RSALMWFGVI FIRHGLYQDG 

       130        140        150        160        170        180 
VFKFTVYIPD NYPDGDCPRL VFDIPVFHPL VDPTSGELDV KRAFAKWRRN HNHIWQVLMY 

       190        200        210        220        230        240 
ARRVFYKIDT ASPLNPEAAV LYEKDIQLFK SKVVDSVKVC TARLFDQPKI EDPYAISFSP 

       250        260        270        280        290 
WNPSVHDEAR EKMLTQKKPE EQHNKSVHVA GLSWVKPGSV QPFSKEEKTV AT 

« Hide

Isoform 2 [UniParc].

Checksum: CE781BABE3F9695C
Show »

FASTA29333,256

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Ovary.
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[3]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Dermoid cancer.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Skin.
[6]"Regulation of apoptosis by the Ft1 protein, a new modulator of protein kinase B/Akt."
Remy I., Michnick S.W.
Mol. Cell. Biol. 24:1493-1504(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH AKT1, SUBCELLULAR LOCATION.
[7]"An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex."
Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W.
Mol. Biol. Cell 19:5059-5071(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, ASSOCIATION WITH THE HOPS COMPLEX, MUTAGENESIS OF 106-TRP-PHE-107.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK023320 mRNA. Translation: BAB14524.1.
CR457308 mRNA. Translation: CAG33589.1.
AK222743 mRNA. Translation: BAD96463.1.
CH471092 Genomic DNA. Translation: EAW82805.1.
BC001134 mRNA. Translation: AAH01134.1.
BC095401 mRNA. Translation: AAH95401.1.
RefSeqNP_001012398.1. NM_001012398.1.
NP_071921.1. NM_022476.2.
XP_005256151.1. XM_005256094.1.
XP_005256152.1. XM_005256095.1.
XP_005256153.1. XM_005256096.1.
XP_005256154.1. XM_005256097.1.
XP_005256155.1. XM_005256098.1.
UniGeneHs.380897.

3D structure databases

ProteinModelPortalQ9H8T0.
SMRQ9H8T0. Positions 78-223.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122157. 28 interactions.
IntActQ9H8T0. 28 interactions.
STRING9606.ENSP00000300245.

PTM databases

PhosphoSiteQ9H8T0.

Polymorphism databases

DMDM54035954.

Proteomic databases

PaxDbQ9H8T0.
PRIDEQ9H8T0.

Protocols and materials databases

DNASU64400.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000300245; ENSP00000300245; ENSG00000166971. [Q9H8T0-2]
ENST00000394657; ENSP00000378152; ENSG00000166971. [Q9H8T0-1]
ENST00000570004; ENSP00000455874; ENSG00000166971. [Q9H8T0-1]
GeneID64400.
KEGGhsa:64400.
UCSCuc002ehk.3. human. [Q9H8T0-1]
uc002ehm.3. human. [Q9H8T0-2]

Organism-specific databases

CTD64400.
GeneCardsGC16M053524.
HGNCHGNC:16710. AKTIP.
HPAHPA041794.
HPA046300.
MIM608483. gene.
neXtProtNX_Q9H8T0.
PharmGKBPA162376210.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG285076.
HOGENOMHOG000033885.
HOVERGENHBG095637.
InParanoidQ9H8T0.
OrthoDBEOG7QG45C.
PhylomeDBQ9H8T0.
TreeFamTF314386.

Enzyme and pathway databases

SignaLinkQ9H8T0.

Gene expression databases

ArrayExpressQ9H8T0.
BgeeQ9H8T0.
CleanExHS_AKTIP.
GenevestigatorQ9H8T0.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiAKTIP.
GenomeRNAi64400.
NextBio66346.
PROQ9H8T0.
SOURCESearch...

Entry information

Entry nameAKTIP_HUMAN
AccessionPrimary (citable) accession number: Q9H8T0
Secondary accession number(s): Q503B1, Q53H38
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2001
Last modified: March 19, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM