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Protein

MOB kinase activator 1A

Gene

MOB1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79Zinc1
Metal bindingi84Zinc1
Metal bindingi161Zinc1
Metal bindingi166Zinc1

GO - Molecular functioni

GO - Biological processi

  • hippo signaling Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-2028269. Signaling by Hippo.
SIGNORiQ9H8S9.

Names & Taxonomyi

Protein namesi
Recommended name:
MOB kinase activator 1A
Alternative name(s):
Mob1 alpha
Short name:
Mob1A
Mob1 homolog 1B
Mps one binder kinase activator-like 1B
Gene namesi
Name:MOB1A
Synonyms:C2orf6, MOB4B, MOBK1B, MOBKL1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:16015. MOB1A.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi55233.
OpenTargetsiENSG00000114978.
PharmGKBiPA25894.

Polymorphism and mutation databases

BioMutaiMOB1A.
DMDMi56749356.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001935662 – 216MOB kinase activator 1AAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei12Phosphothreonine1 Publication1
Modified residuei35Phosphothreonine1 Publication1
Modified residuei74Phosphothreonine; by STK3/MST21 Publication1
Modified residuei181Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated by STK3/MST2 and STK4/MST1 and this phosphorylation enhances its binding to LATS1.3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H8S9.
MaxQBiQ9H8S9.
PaxDbiQ9H8S9.
PeptideAtlasiQ9H8S9.
PRIDEiQ9H8S9.

2D gel databases

OGPiQ9H8S9.

PTM databases

iPTMnetiQ9H8S9.
PhosphoSitePlusiQ9H8S9.

Expressioni

Tissue specificityi

Adrenal gland, bone marrow, brain, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, heart, spinal cord, fetal brain and fetal liver.1 Publication

Gene expression databases

BgeeiENSG00000114978.
CleanExiHS_MOBKL1B.
GenevisibleiQ9H8S9. HS.

Organism-specific databases

HPAiHPA047088.

Interactioni

Subunit structurei

Binds STK38 and STK38L. Interacts with LATS1 and LATS2. Forms a tripartite complex with STK38 and STK3/MST2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Q9WMX22EBI-748229,EBI-6863748From a different organism.
CMTM3Q96MX03EBI-748229,EBI-7247651
FAM118AQ9NWS63EBI-748229,EBI-8638992
FAM9BQ8IZU03EBI-748229,EBI-10175124
KANK2Q63ZY35EBI-748229,EBI-2556193
KXD1Q9BQD33EBI-748229,EBI-739657
LATS1O958359EBI-748229,EBI-444209
LATS2Q9NRM74EBI-748229,EBI-3506895
SEPT3Q9UH035EBI-748229,EBI-727037
STK38Q152083EBI-748229,EBI-458376
STK38LQ9Y2H14EBI-748229,EBI-991501
STK4Q130439EBI-748229,EBI-367376
TDO2P487755EBI-748229,EBI-743494

Protein-protein interaction databases

BioGridi120527. 54 interactors.
DIPiDIP-36594N.
IntActiQ9H8S9. 50 interactors.
MINTiMINT-197366.
STRINGi9606.ENSP00000379364.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 15Combined sources4
Turni42 – 45Combined sources4
Helixi53 – 75Combined sources23
Helixi76 – 78Combined sources3
Turni81 – 83Combined sources3
Beta strandi88 – 90Combined sources3
Beta strandi93 – 95Combined sources3
Beta strandi100 – 102Combined sources3
Helixi111 – 126Combined sources16
Turni129 – 131Combined sources3
Beta strandi135 – 137Combined sources3
Helixi144 – 165Combined sources22
Helixi167 – 172Combined sources6
Helixi176 – 193Combined sources18
Helixi198 – 201Combined sources4
Helixi202 – 204Combined sources3
Helixi205 – 211Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PI1X-ray2.00A33-216[»]
4J1VX-ray1.95A/C33-216[»]
4JIZX-ray2.10A40-211[»]
5BRKX-ray2.30A1-216[»]
5BRMX-ray2.65A/B/C/D/E/F41-216[»]
ProteinModelPortaliQ9H8S9.
SMRiQ9H8S9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H8S9.

Family & Domainsi

Sequence similaritiesi

Belongs to the MOB1/phocein family.Curated

Phylogenomic databases

eggNOGiKOG0440. Eukaryota.
ENOG410XSUJ. LUCA.
GeneTreeiENSGT00550000074456.
HOGENOMiHOG000164685.
HOVERGENiHBG052489.
InParanoidiQ9H8S9.
KOiK06685.
OMAiEFCTETS.
OrthoDBiEOG091G0LDA.
PhylomeDBiQ9H8S9.
TreeFamiTF300789.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. MOB_kinase_act_fam.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SMARTiSM01388. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H8S9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFLFSSRSS KTFKPKKNIP EGSHQYELLK HAEATLGSGN LRQAVMLPEG
60 70 80 90 100
EDLNEWIAVN TVDFFNQINM LYGTITEFCT EASCPVMSAG PRYEYHWADG
110 120 130 140 150
TNIKKPIKCS APKYIDYLMT WVQDQLDDET LFPSKIGVPF PKNFMSVAKT
160 170 180 190 200
ILKRLFRVYA HIYHQHFDSV MQLQEEAHLN TSFKHFIFFV QEFNLIDRRE
210
LAPLQELIEK LGSKDR
Length:216
Mass (Da):25,080
Last modified:January 23, 2007 - v4
Checksum:i58043AECAD1F5987
GO
Isoform 2 (identifier: Q9H8S9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     138-148: VPFPKNFMSVA → ELTLSKYSFFF
     149-216: Missing.

Note: May be due to an intron retention.
Show »
Length:148
Mass (Da):16,928
Checksum:i70F00C42359120F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5F → L in BAB14525 (PubMed:14702039).Curated1
Sequence conflicti176E → G in BAA91810 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012295138 – 148VPFPKNFMSVA → ELTLSKYSFFF in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_012296149 – 216Missing in isoform 2. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016839 mRNA. Translation: BAB19058.1.
AJ577474 mRNA. Translation: CAE12093.1.
AK001650 mRNA. Translation: BAA91810.1.
AK021657 mRNA. Translation: BAB13868.1.
AK023321 mRNA. Translation: BAB14525.1.
AC073263 Genomic DNA. Translation: AAX93060.1.
BC003398 mRNA. Translation: AAH03398.1.
CCDSiCCDS46340.1. [Q9H8S9-1]
RefSeqiNP_001304039.1. NM_001317110.1.
NP_001304040.1. NM_001317111.1.
NP_001304041.1. NM_001317112.1.
NP_060691.2. NM_018221.4. [Q9H8S9-1]
UniGeneiHs.602092.

Genome annotation databases

EnsembliENST00000396049; ENSP00000379364; ENSG00000114978. [Q9H8S9-1]
GeneIDi55233.
KEGGihsa:55233.
UCSCiuc002skh.5. human. [Q9H8S9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016839 mRNA. Translation: BAB19058.1.
AJ577474 mRNA. Translation: CAE12093.1.
AK001650 mRNA. Translation: BAA91810.1.
AK021657 mRNA. Translation: BAB13868.1.
AK023321 mRNA. Translation: BAB14525.1.
AC073263 Genomic DNA. Translation: AAX93060.1.
BC003398 mRNA. Translation: AAH03398.1.
CCDSiCCDS46340.1. [Q9H8S9-1]
RefSeqiNP_001304039.1. NM_001317110.1.
NP_001304040.1. NM_001317111.1.
NP_001304041.1. NM_001317112.1.
NP_060691.2. NM_018221.4. [Q9H8S9-1]
UniGeneiHs.602092.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PI1X-ray2.00A33-216[»]
4J1VX-ray1.95A/C33-216[»]
4JIZX-ray2.10A40-211[»]
5BRKX-ray2.30A1-216[»]
5BRMX-ray2.65A/B/C/D/E/F41-216[»]
ProteinModelPortaliQ9H8S9.
SMRiQ9H8S9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120527. 54 interactors.
DIPiDIP-36594N.
IntActiQ9H8S9. 50 interactors.
MINTiMINT-197366.
STRINGi9606.ENSP00000379364.

PTM databases

iPTMnetiQ9H8S9.
PhosphoSitePlusiQ9H8S9.

Polymorphism and mutation databases

BioMutaiMOB1A.
DMDMi56749356.

2D gel databases

OGPiQ9H8S9.

Proteomic databases

EPDiQ9H8S9.
MaxQBiQ9H8S9.
PaxDbiQ9H8S9.
PeptideAtlasiQ9H8S9.
PRIDEiQ9H8S9.

Protocols and materials databases

DNASUi55233.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000396049; ENSP00000379364; ENSG00000114978. [Q9H8S9-1]
GeneIDi55233.
KEGGihsa:55233.
UCSCiuc002skh.5. human. [Q9H8S9-1]

Organism-specific databases

CTDi55233.
DisGeNETi55233.
GeneCardsiMOB1A.
HGNCiHGNC:16015. MOB1A.
HPAiHPA047088.
MIMi609281. gene.
neXtProtiNX_Q9H8S9.
OpenTargetsiENSG00000114978.
PharmGKBiPA25894.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0440. Eukaryota.
ENOG410XSUJ. LUCA.
GeneTreeiENSGT00550000074456.
HOGENOMiHOG000164685.
HOVERGENiHBG052489.
InParanoidiQ9H8S9.
KOiK06685.
OMAiEFCTETS.
OrthoDBiEOG091G0LDA.
PhylomeDBiQ9H8S9.
TreeFamiTF300789.

Enzyme and pathway databases

ReactomeiR-HSA-2028269. Signaling by Hippo.
SIGNORiQ9H8S9.

Miscellaneous databases

EvolutionaryTraceiQ9H8S9.
GeneWikiiMOBKL1B.
GenomeRNAii55233.
PROiQ9H8S9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114978.
CleanExiHS_MOBKL1B.
GenevisibleiQ9H8S9. HS.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. MOB_kinase_act_fam.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SMARTiSM01388. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMOB1A_HUMAN
AccessioniPrimary (citable) accession number: Q9H8S9
Secondary accession number(s): Q53S34
, Q9H3T5, Q9HAI0, Q9NVE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 140 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.