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Protein

Ubiquitin carboxyl-terminal hydrolase MINDY-3

Gene

MINDY3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei51NucleophileBy similarity1
Active sitei287Proton acceptorBy similarity1

GO - Molecular functioni

  • cysteine-type carboxypeptidase activity Source: UniProtKB
  • Lys48-specific deubiquitinase activity Source: UniProtKB
  • thiol-dependent ubiquitinyl hydrolase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processApoptosis, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase MINDY-3 (EC:3.4.19.12)
Alternative name(s):
Dermal papilla-derived protein 5
Deubiquitinating enzyme MINDY-3
Protein CARP
Gene namesi
Name:MINDY3Imported
Synonyms:C10orf97, CARP, DERP5, FAM188A
ORF Names:MSTP126, My042
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:23578. MINDY3.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi80013.
MalaCardsiFAM188A.
OpenTargetsiENSG00000148481.
PharmGKBiPA165548562.

Polymorphism and mutation databases

BioMutaiFAM188A.
DMDMi74761533.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003175601 – 445Ubiquitin carboxyl-terminal hydrolase MINDY-3Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei125PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H8M7.
MaxQBiQ9H8M7.
PaxDbiQ9H8M7.
PeptideAtlasiQ9H8M7.
PRIDEiQ9H8M7.

PTM databases

iPTMnetiQ9H8M7.
PhosphoSitePlusiQ9H8M7.

Expressioni

Tissue specificityi

Widely expressed with high levels in heart, skeletal muscle, and kidney, and low levels in liver and brain (PubMed:12054670). Also expressed in lung (at protein level) (PubMed:21499297).2 Publications

Gene expression databases

BgeeiENSG00000148481.
ExpressionAtlasiQ9H8M7. baseline and differential.
GenevisibleiQ9H8M7. HS.

Organism-specific databases

HPAiHPA038400.
HPA038406.

Interactioni

Subunit structurei

Interacts with COPS5.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi123069. 28 interactors.
IntActiQ9H8M7. 10 interactors.
MINTiMINT-1412937.
STRINGi9606.ENSP00000277632.

Structurei

3D structure databases

ProteinModelPortaliQ9H8M7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2871. Eukaryota.
ENOG410XSGV. LUCA.
GeneTreeiENSGT00530000063283.
HOVERGENiHBG061682.
InParanoidiQ9H8M7.
OMAiPLIHSTY.
OrthoDBiEOG091G0TF1.
PhylomeDBiQ9H8M7.
TreeFamiTF323996.

Family and domain databases

InterProiView protein in InterPro
IPR025257. DUF4205.
IPR011992. EF-hand-dom_pair.
PfamiView protein in Pfam
PF13898. DUF4205. 1 hit.
SMARTiView protein in SMART
SM01174. DUF4205. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H8M7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSELTKELME LVWGTKSSPG LSDTIFCRWT QGFVFSESEG SALEQFEGGP
60 70 80 90 100
CAVIAPVQAF LLKKLLFSSE KSSWRDCSEE EQKELLCHTL CDILESACCD
110 120 130 140 150
HSGSYCLVSW LRGKTTEETA SISGSPAESS CQVEHSSALA VEELGFERFH
160 170 180 190 200
ALIQKRSFRS LPELKDAVLD QYSMWGNKFG VLLFLYSVLL TKGIENIKNE
210 220 230 240 250
IEDASEPLID PVYGHGSQSL INLLLTGHAV SNVWDGDREC SGMKLLGIHE
260 270 280 290 300
QAAVGFLTLM EALRYCKVGS YLKSPKFPIW IVGSETHLTV FFAKDMALVA
310 320 330 340 350
PEAPSEQARR VFQTYDPEDN GFIPDSLLED VMKALDLVSD PEYINLMKNK
360 370 380 390 400
LDPEGLGIIL LGPFLQEFFP DQGSSGPESF TVYHYNGLKQ SNYNEKVMYV
410 420 430 440
EGTAVVMGFE DPMLQTDDTP IKRCLQTKWP YIELLWTTDR SPSLN
Length:445
Mass (Da):49,725
Last modified:March 1, 2001 - v1
Checksum:iF30B4A5E357AF52E
GO
Isoform 2 (identifier: Q9H8M7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-445: Missing.

Show »
Length:136
Mass (Da):14,942
Checksum:iFB29CAFB7F8C68AB
GO

Sequence cautioni

The sequence AAG43159 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH61585 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAQ13660 differs from that shown. Reason: Frameshift at position 111.Curated
The sequence CAI13817 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI13818 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI13819 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence EAW86233 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31Q → R in CAG33596 (Ref. 5) Curated1
Sequence conflicti96S → G in AAH20605 (PubMed:15489334).Curated1
Sequence conflicti111L → V in AAQ13660 (Ref. 6) Curated1
Sequence conflicti116T → A in CAG33596 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031039137 – 445Missing in isoform 2. 2 PublicationsAdd BLAST309

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014761 mRNA. Translation: BAB87802.1.
AK023459 mRNA. Translation: BAB14582.1.
AL833873 mRNA. Translation: CAD38730.1.
CR457315 mRNA. Translation: CAG33596.1.
AF176916 mRNA. Translation: AAQ13660.1. Frameshift.
AL590439 Genomic DNA. Translation: CAI13817.1. Sequence problems.
AL590439 Genomic DNA. Translation: CAI13818.1. Sequence problems.
AL590439 Genomic DNA. Translation: CAI13819.1. Sequence problems.
AL590439 Genomic DNA. Translation: CAI13820.1.
CH471072 Genomic DNA. Translation: EAW86233.1. Sequence problems.
CH471072 Genomic DNA. Translation: EAW86234.1.
BC020605 mRNA. Translation: AAH20605.1.
BC061585 mRNA. Translation: AAH61585.1. Sequence problems.
BC067799 mRNA. Translation: AAH67799.1.
AF063600 mRNA. Translation: AAG43159.1. Different initiation.
CCDSiCCDS7110.1. [Q9H8M7-1]
PIRiJC7861.
RefSeqiNP_001305259.1. NM_001318330.1.
NP_079224.1. NM_024948.3. [Q9H8M7-1]
XP_005252657.1. XM_005252600.2.
XP_005252659.1. XM_005252602.2.
XP_011517996.1. XM_011519694.1.
XP_016872162.1. XM_017016673.1.
UniGeneiHs.158870.

Genome annotation databases

EnsembliENST00000277632; ENSP00000277632; ENSG00000148481. [Q9H8M7-1]
GeneIDi80013.
KEGGihsa:80013.
UCSCiuc001iod.2. human. [Q9H8M7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014761 mRNA. Translation: BAB87802.1.
AK023459 mRNA. Translation: BAB14582.1.
AL833873 mRNA. Translation: CAD38730.1.
CR457315 mRNA. Translation: CAG33596.1.
AF176916 mRNA. Translation: AAQ13660.1. Frameshift.
AL590439 Genomic DNA. Translation: CAI13817.1. Sequence problems.
AL590439 Genomic DNA. Translation: CAI13818.1. Sequence problems.
AL590439 Genomic DNA. Translation: CAI13819.1. Sequence problems.
AL590439 Genomic DNA. Translation: CAI13820.1.
CH471072 Genomic DNA. Translation: EAW86233.1. Sequence problems.
CH471072 Genomic DNA. Translation: EAW86234.1.
BC020605 mRNA. Translation: AAH20605.1.
BC061585 mRNA. Translation: AAH61585.1. Sequence problems.
BC067799 mRNA. Translation: AAH67799.1.
AF063600 mRNA. Translation: AAG43159.1. Different initiation.
CCDSiCCDS7110.1. [Q9H8M7-1]
PIRiJC7861.
RefSeqiNP_001305259.1. NM_001318330.1.
NP_079224.1. NM_024948.3. [Q9H8M7-1]
XP_005252657.1. XM_005252600.2.
XP_005252659.1. XM_005252602.2.
XP_011517996.1. XM_011519694.1.
XP_016872162.1. XM_017016673.1.
UniGeneiHs.158870.

3D structure databases

ProteinModelPortaliQ9H8M7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123069. 28 interactors.
IntActiQ9H8M7. 10 interactors.
MINTiMINT-1412937.
STRINGi9606.ENSP00000277632.

PTM databases

iPTMnetiQ9H8M7.
PhosphoSitePlusiQ9H8M7.

Polymorphism and mutation databases

BioMutaiFAM188A.
DMDMi74761533.

Proteomic databases

EPDiQ9H8M7.
MaxQBiQ9H8M7.
PaxDbiQ9H8M7.
PeptideAtlasiQ9H8M7.
PRIDEiQ9H8M7.

Protocols and materials databases

DNASUi80013.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000277632; ENSP00000277632; ENSG00000148481. [Q9H8M7-1]
GeneIDi80013.
KEGGihsa:80013.
UCSCiuc001iod.2. human. [Q9H8M7-1]

Organism-specific databases

CTDi80013.
DisGeNETi80013.
GeneCardsiFAM188A.
HGNCiHGNC:23578. MINDY3.
HPAiHPA038400.
HPA038406.
MalaCardsiFAM188A.
MIMi611649. gene.
neXtProtiNX_Q9H8M7.
OpenTargetsiENSG00000148481.
PharmGKBiPA165548562.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2871. Eukaryota.
ENOG410XSGV. LUCA.
GeneTreeiENSGT00530000063283.
HOVERGENiHBG061682.
InParanoidiQ9H8M7.
OMAiPLIHSTY.
OrthoDBiEOG091G0TF1.
PhylomeDBiQ9H8M7.
TreeFamiTF323996.

Miscellaneous databases

ChiTaRSiFAM188A. human.
GenomeRNAii80013.
PROiPR:Q9H8M7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148481.
ExpressionAtlasiQ9H8M7. baseline and differential.
GenevisibleiQ9H8M7. HS.

Family and domain databases

InterProiView protein in InterPro
IPR025257. DUF4205.
IPR011992. EF-hand-dom_pair.
PfamiView protein in Pfam
PF13898. DUF4205. 1 hit.
SMARTiView protein in SMART
SM01174. DUF4205. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMINY3_HUMAN
AccessioniPrimary (citable) accession number: Q9H8M7
Secondary accession number(s): Q5SZ68
, Q5SZ69, Q5SZ70, Q6IA40, Q6P7P0, Q7Z2S1, Q8WUF1, Q9H3I4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2001
Last modified: June 7, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was named CARP for 'CARD domain-containing protein' by PubMed:12054670. However, no CARD domain is detected by any prediction tool.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.