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Protein

Bromodomain-containing protein 9

Gene

BRD9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in chromatin remodeling and regulation of transcription.

GO - Molecular functioni

  • lysine-acetylated histone binding Source: UniProtKB
  • nucleic acid binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 9
Alternative name(s):
Rhabdomyosarcoma antigen MU-RMS-40.8
Gene namesi
Name:BRD9
ORF Names:UNQ3040/PRO9856
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:25818. BRD9.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134866578.

Chemistry

ChEMBLiCHEMBL3108640.
GuidetoPHARMACOLOGYi2728.

Polymorphism and mutation databases

BioMutaiBRD9.
DMDMi239938605.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 597597Bromodomain-containing protein 9PRO_0000239219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561PhosphoserineBy similarity
Modified residuei373 – 3731N6-acetyllysineCombined sources
Modified residuei566 – 5661PhosphoserineCombined sources
Modified residuei588 – 5881PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H8M2.
MaxQBiQ9H8M2.
PaxDbiQ9H8M2.
PeptideAtlasiQ9H8M2.
PRIDEiQ9H8M2.
TopDownProteomicsiQ9H8M2-1. [Q9H8M2-1]
Q9H8M2-4. [Q9H8M2-4]

PTM databases

iPTMnetiQ9H8M2.
PhosphoSiteiQ9H8M2.

Expressioni

Gene expression databases

BgeeiENSG00000028310.
CleanExiHS_BRD9.
ExpressionAtlasiQ9H8M2. baseline and differential.
GenevisibleiQ9H8M2. HS.

Organism-specific databases

HPAiHPA021465.

Interactioni

Subunit structurei

Binds acetylated histone H3 (in vitro).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CATIPQ7Z7H33EBI-10258305,EBI-10258233

GO - Molecular functioni

  • lysine-acetylated histone binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122430. 11 interactions.
IntActiQ9H8M2. 3 interactions.
STRINGi9606.ENSP00000419765.

Chemistry

BindingDBiQ9H8M2.

Structurei

Secondary structure

1
597
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi140 – 15314Combined sources
Turni160 – 1623Combined sources
Turni167 – 1693Combined sources
Helixi173 – 1764Combined sources
Helixi183 – 1919Combined sources
Helixi198 – 21518Combined sources
Beta strandi218 – 2203Combined sources
Helixi221 – 23717Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HMEX-ray2.23A/B134-239[»]
4NQNX-ray1.73A134-239[»]
4UITX-ray1.30A134-238[»]
4UIUX-ray1.64A134-238[»]
4UIVX-ray1.72A134-238[»]
4UIWX-ray1.73A134-238[»]
4XY8X-ray1.70A134-239[»]
4YY4X-ray1.47A134-239[»]
4YY6X-ray1.45A134-239[»]
4YYDX-ray1.52A134-239[»]
4YYGX-ray2.10A134-239[»]
4YYHX-ray1.74A/B134-239[»]
4YYIX-ray1.50A/B/D/E134-239[»]
4YYJX-ray1.85A/B/D/E134-239[»]
4YYKX-ray1.79A/B/D/E134-239[»]
4Z6HX-ray1.80A/B134-239[»]
4Z6IX-ray1.95A/B134-239[»]
5E9VX-ray1.80A/B134-239[»]
5EU1X-ray1.60A/B134-239[»]
5F1HX-ray1.82A/B134-239[»]
5F1LX-ray2.30A/B134-239[»]
5F25X-ray1.68A/B134-239[»]
5F2PX-ray1.80A/B134-239[»]
5IGNX-ray1.70A/B134-239[»]
5JI8X-ray1.42A137-239[»]
ProteinModelPortaliQ9H8M2.
SMRiQ9H8M2. Positions 138-238.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H8M2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini153 – 22371BromoPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi64 – 9532Lys-richAdd
BLAST

Domaini

Bromo domains mediate interaction with histones that have acetylated lysine residues at specific positions.1 Publication

Sequence similaritiesi

Contains 1 bromo domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain

Phylogenomic databases

eggNOGiKOG1828. Eukaryota.
ENOG410XRRI. LUCA.
GeneTreeiENSGT00530000063939.
HOGENOMiHOG000070022.
HOVERGENiHBG107536.
InParanoidiQ9H8M2.
KOiK11723.
OMAiIAANEYK.
OrthoDBiEOG091G06S4.
PhylomeDBiQ9H8M2.
TreeFamiTF106439.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
InterProiIPR001487. Bromodomain.
IPR021900. DUF3512.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF12024. DUF3512. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H8M2-5) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKKHKKHKA EWRSSYEDYA DKPLEKPLKL VLKVGGSEVT ELSGSGHDSS
60 70 80 90 100
YYDDRSDHER ERHKEKKKKK KKKSEKEKHL DDEERRKRKE EKKRKREREH
110 120 130 140 150
CDTEGEADDF DPGKKVEVEP PPDRPVRACR TQPAENESTP IQQLLEHFLR
160 170 180 190 200
QLQRKDPHGF FAFPVTDAIA PGYSMIIKHP MDFGTMKDKI VANEYKSVTE
210 220 230 240 250
FKADFKLMCD NAMTYNRPDT VYYKLAKKIL HAGFKMMSKQ AALLGNEDTA
260 270 280 290 300
VEEPVPEVVP VQVETAKKSK KPSREVISCM FEPEGNACSL TDSTAEEHVL
310 320 330 340 350
ALVEHAADEA RDRINRFLPG GKMGYLKRNG DGSLLYSVVN TAEPDADEEE
360 370 380 390 400
THPVDLSSLS SKLLPGFTTL GFKDERRNKV TFLSSATTAL SMQNNSVFGD
410 420 430 440 450
LKSDEMELLY SAYGDETGVQ CALSLQEFVK DAGSYSKKVV DDLLDQITGG
460 470 480 490 500
DHSRTLFQLK QRRNVPMKPP DEAKVGDTLG DSSSSVLEFM SMKSYPDVSV
510 520 530 540 550
DISMLSSLGK VKKELDPDDS HLNLDETTKL LQDLHEAQAE RGGSRPSSNL
560 570 580 590
SSLSNASERD QHHLGSPSRL SVGEQPDVTH DPYEFLQSPE PAASAKT
Length:597
Mass (Da):67,000
Last modified:June 16, 2009 - v2
Checksum:i3B248FA97948266E
GO
Isoform 2 (identifier: Q9H8M2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-391: Missing.

Note: No experimental confirmation available.
Show »
Length:206
Mass (Da):22,534
Checksum:i05D72FAD8109A693
GO
Isoform 3 (identifier: Q9H8M2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     117-133: EVEPPPDRPVRACRTQP → MKGYQSLVFNFFFLKLS

Note: No experimental confirmation available.
Show »
Length:481
Mass (Da):53,249
Checksum:i4961C172226405D2
GO
Isoform 4 (identifier: Q9H8M2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     117-133: EVEPPPDRPVRACRTQP → MKGYQSLVFNFFFLKLS
     323-349: MGYLKRNGDGSLLYSVVNTAEPDADEE → VVLCGHRERVPRGPRLSVCLSFWVGAV
     350-597: Missing.

Note: No experimental confirmation available.
Show »
Length:233
Mass (Da):26,247
Checksum:i7043139E52BD6B40
GO
Isoform 5 (identifier: Q9H8M2-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     117-133: EVEPPPDRPVRACRTQP → MKGYQSLVFNFFFLKLS
     239-239: K → KERLLALKRSMSFMQDMDFSQ

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:501
Mass (Da):55,665
Checksum:i5C68F53097BA073C
GO
Isoform 6 (identifier: Q9H8M2-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.
     117-155: EVEPPPDRPV...EHFLRQLQRK → MMTGQTMSER...SWNTSSASFR

Note: No experimental confirmation available.
Show »
Length:544
Mass (Da):60,763
Checksum:i00E885F57A1EC36B
GO

Sequence cautioni

The sequence BAB15565 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti146 – 1461E → D in BAB14907 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti170 – 1701A → T.
Corresponds to variant rs34292369 [ dbSNP | Ensembl ].
VAR_059143
Natural varianti266 – 2661A → T.
Corresponds to variant rs34292369 [ dbSNP | Ensembl ].
VAR_033635
Natural varianti389 – 3891A → T.
Corresponds to variant rs414349 [ dbSNP | Ensembl ].
VAR_033636

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 391391Missing in isoform 2. 1 PublicationVSP_019112Add
BLAST
Alternative sequencei1 – 116116Missing in isoform 3, isoform 4, isoform 5 and isoform 6. 3 PublicationsVSP_037493Add
BLAST
Alternative sequencei117 – 15539EVEPP…QLQRK → MMTGQTMSERGTKKRKRRRR RSPRRRSIWTMRKEGSERKR RSGSERGSTVTRRERLTTLI LGRRWRWSRPQIGQSERAGH SQPKMRAHLFSNSWNTSSAS FR in isoform 6. 1 PublicationVSP_043234Add
BLAST
Alternative sequencei117 – 13317EVEPP…CRTQP → MKGYQSLVFNFFFLKLS in isoform 3, isoform 4 and isoform 5. 3 PublicationsVSP_037494Add
BLAST
Alternative sequencei239 – 2391K → KERLLALKRSMSFMQDMDFS Q in isoform 5. 1 PublicationVSP_019113
Alternative sequencei323 – 34927MGYLK…DADEE → VVLCGHRERVPRGPRLSVCL SFWVGAV in isoform 4. 1 PublicationVSP_019114Add
BLAST
Alternative sequencei350 – 597248Missing in isoform 4. 1 PublicationVSP_019115Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358630 mRNA. Translation: AAQ88993.1.
AK023503 mRNA. Translation: BAB14591.1.
AK024392 mRNA. Translation: BAB14907.1.
AK026830 mRNA. Translation: BAB15565.1. Different initiation.
AK297573 mRNA. Translation: BAG59964.1.
AK299157 mRNA. Translation: BAG61205.1.
AC122719 Genomic DNA. No translation available.
CH471102 Genomic DNA. Translation: EAX08192.1.
BC041590 mRNA. Translation: AAH41590.1.
DQ248311 mRNA. Translation: ABB55266.1.
CCDSiCCDS34127.2. [Q9H8M2-5]
CCDS34128.2. [Q9H8M2-6]
RefSeqiNP_001009877.2. NM_001009877.2. [Q9H8M2-6]
NP_001304880.1. NM_001317951.1. [Q9H8M2-1]
NP_076413.3. NM_023924.4. [Q9H8M2-5]
UniGeneiHs.449278.

Genome annotation databases

EnsembliENST00000467963; ENSP00000419765; ENSG00000028310. [Q9H8M2-5]
ENST00000483173; ENSP00000419845; ENSG00000028310. [Q9H8M2-6]
GeneIDi65980.
KEGGihsa:65980.
UCSCiuc003jbq.4. human. [Q9H8M2-5]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358630 mRNA. Translation: AAQ88993.1.
AK023503 mRNA. Translation: BAB14591.1.
AK024392 mRNA. Translation: BAB14907.1.
AK026830 mRNA. Translation: BAB15565.1. Different initiation.
AK297573 mRNA. Translation: BAG59964.1.
AK299157 mRNA. Translation: BAG61205.1.
AC122719 Genomic DNA. No translation available.
CH471102 Genomic DNA. Translation: EAX08192.1.
BC041590 mRNA. Translation: AAH41590.1.
DQ248311 mRNA. Translation: ABB55266.1.
CCDSiCCDS34127.2. [Q9H8M2-5]
CCDS34128.2. [Q9H8M2-6]
RefSeqiNP_001009877.2. NM_001009877.2. [Q9H8M2-6]
NP_001304880.1. NM_001317951.1. [Q9H8M2-1]
NP_076413.3. NM_023924.4. [Q9H8M2-5]
UniGeneiHs.449278.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HMEX-ray2.23A/B134-239[»]
4NQNX-ray1.73A134-239[»]
4UITX-ray1.30A134-238[»]
4UIUX-ray1.64A134-238[»]
4UIVX-ray1.72A134-238[»]
4UIWX-ray1.73A134-238[»]
4XY8X-ray1.70A134-239[»]
4YY4X-ray1.47A134-239[»]
4YY6X-ray1.45A134-239[»]
4YYDX-ray1.52A134-239[»]
4YYGX-ray2.10A134-239[»]
4YYHX-ray1.74A/B134-239[»]
4YYIX-ray1.50A/B/D/E134-239[»]
4YYJX-ray1.85A/B/D/E134-239[»]
4YYKX-ray1.79A/B/D/E134-239[»]
4Z6HX-ray1.80A/B134-239[»]
4Z6IX-ray1.95A/B134-239[»]
5E9VX-ray1.80A/B134-239[»]
5EU1X-ray1.60A/B134-239[»]
5F1HX-ray1.82A/B134-239[»]
5F1LX-ray2.30A/B134-239[»]
5F25X-ray1.68A/B134-239[»]
5F2PX-ray1.80A/B134-239[»]
5IGNX-ray1.70A/B134-239[»]
5JI8X-ray1.42A137-239[»]
ProteinModelPortaliQ9H8M2.
SMRiQ9H8M2. Positions 138-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122430. 11 interactions.
IntActiQ9H8M2. 3 interactions.
STRINGi9606.ENSP00000419765.

Chemistry

BindingDBiQ9H8M2.
ChEMBLiCHEMBL3108640.
GuidetoPHARMACOLOGYi2728.

PTM databases

iPTMnetiQ9H8M2.
PhosphoSiteiQ9H8M2.

Polymorphism and mutation databases

BioMutaiBRD9.
DMDMi239938605.

Proteomic databases

EPDiQ9H8M2.
MaxQBiQ9H8M2.
PaxDbiQ9H8M2.
PeptideAtlasiQ9H8M2.
PRIDEiQ9H8M2.
TopDownProteomicsiQ9H8M2-1. [Q9H8M2-1]
Q9H8M2-4. [Q9H8M2-4]

Protocols and materials databases

DNASUi65980.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000467963; ENSP00000419765; ENSG00000028310. [Q9H8M2-5]
ENST00000483173; ENSP00000419845; ENSG00000028310. [Q9H8M2-6]
GeneIDi65980.
KEGGihsa:65980.
UCSCiuc003jbq.4. human. [Q9H8M2-5]

Organism-specific databases

CTDi65980.
GeneCardsiBRD9.
HGNCiHGNC:25818. BRD9.
HPAiHPA021465.
neXtProtiNX_Q9H8M2.
PharmGKBiPA134866578.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1828. Eukaryota.
ENOG410XRRI. LUCA.
GeneTreeiENSGT00530000063939.
HOGENOMiHOG000070022.
HOVERGENiHBG107536.
InParanoidiQ9H8M2.
KOiK11723.
OMAiIAANEYK.
OrthoDBiEOG091G06S4.
PhylomeDBiQ9H8M2.
TreeFamiTF106439.

Miscellaneous databases

ChiTaRSiBRD9. human.
EvolutionaryTraceiQ9H8M2.
GenomeRNAii65980.
PROiQ9H8M2.

Gene expression databases

BgeeiENSG00000028310.
CleanExiHS_BRD9.
ExpressionAtlasiQ9H8M2. baseline and differential.
GenevisibleiQ9H8M2. HS.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
InterProiIPR001487. Bromodomain.
IPR021900. DUF3512.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF12024. DUF3512. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRD9_HUMAN
AccessioniPrimary (citable) accession number: Q9H8M2
Secondary accession number(s): A6NFY8
, B4DMQ2, B4DR93, Q2XUS1, Q6UWU9, Q8IUS4, Q9H5Q5, Q9H7R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 16, 2009
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.