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Protein

Probable ATP-dependent RNA helicase DDX31

Gene

DDX31

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable ATP-dependent RNA helicase (By similarity). Plays a role in ribosome biogenesis and TP53/p53 regulation through its interaction with NPM1 (PubMed:23019224).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi275 – 282ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • ribosome biogenesis Source: UniProtKB
  • RNA secondary structure unwinding Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX31 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 31
Helicain
Gene namesi
Name:DDX31
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:16715. DDX31.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: HPA
  • intracellular membrane-bounded organelle Source: HPA
  • nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi64794.
OpenTargetsiENSG00000125485.
PharmGKBiPA27218.

Polymorphism and mutation databases

BioMutaiDDX31.
DMDMi71153334.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550491 – 851Probable ATP-dependent RNA helicase DDX31Add BLAST851

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei828Omega-N-methylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

EPDiQ9H8H2.
MaxQBiQ9H8H2.
PaxDbiQ9H8H2.
PeptideAtlasiQ9H8H2.
PRIDEiQ9H8H2.

2D gel databases

SWISS-2DPAGEQ9H8H2.

PTM databases

iPTMnetiQ9H8H2.
PhosphoSitePlusiQ9H8H2.

Expressioni

Tissue specificityi

Weakly or undetectably expressed in normal organs. Up-regulated in renal cell carcinoma.1 Publication

Gene expression databases

BgeeiENSG00000125485.
CleanExiHS_DDX31.
ExpressionAtlasiQ9H8H2. baseline and differential.
GenevisibleiQ9H8H2. HS.

Organism-specific databases

HPAiHPA020891.

Interactioni

Subunit structurei

Interacts with NPM1; this interaction prevents interaction between NPM1 and HDM2.1 Publication

Protein-protein interaction databases

BioGridi122302. 105 interactors.
IntActiQ9H8H2. 5 interactors.
MINTiMINT-3056737.
STRINGi9606.ENSP00000361232.

Structurei

3D structure databases

ProteinModelPortaliQ9H8H2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini262 – 443Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST182
Domaini480 – 659Helicase C-terminalPROSITE-ProRule annotationAdd BLAST180

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi230 – 259Q motifAdd BLAST30
Motifi388 – 391DEAD box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0348. Eukaryota.
ENOG410XNT7. LUCA.
GeneTreeiENSGT00550000075041.
HOVERGENiHBG103793.
InParanoidiQ9H8H2.
KOiK14806.
OMAiRDDCFKG.
OrthoDBiEOG091G06RP.
PhylomeDBiQ9H8H2.
TreeFamiTF323273.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H8H2-1) [UniParc]FASTAAdd to basket
Also known as: Helicain B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPDLASQRH SESFPSVNSR PNVILPGREG RREGLPPGGG TRGSLVPTRP
60 70 80 90 100
VPPSPAPLGT SPYSWSRSGP GRGGGAGSSR VPRGVPGPAV CAPGSLLHHA
110 120 130 140 150
SPTQTMAAAD GSLFDNPRTF SRRPPAQASR QAKATKRKYQ ASSEAPPAKR
160 170 180 190 200
RNETSFLPAK KTSVKETQRT FKGNAQKMFS PKKHSVSTSD RNQEERQCIK
210 220 230 240 250
TSSLFKNNPD IPELHRPVVK QVQEKVFTSA AFHELGLHPH LISTINTVLK
260 270 280 290 300
MSSMTSVQKQ SIPVLLEGRD ALVRSQTGSG KTLAYCIPVV QSLQAMESKI
310 320 330 340 350
QRSDGPYALV LVPTRELALQ SFDTVQKLLK PFTWIVPGVL MGGEKRKSEK
360 370 380 390 400
ARLRKGINIL ISTPGRLVDH IKSTKNIHFS RLRWLVFDEA DRILDLGFEK
410 420 430 440 450
DITVILNAVN AECQKRQNVL LSATLTEGVT RLADISLHDP VSISVLDKSH
460 470 480 490 500
DQLNPKDKAV QEVCPPPAGD KLDSFAIPES LKQHVTVVPS KLRLVCLAAF
510 520 530 540 550
ILQKCKFEED QKMVVFFSSC ELVEFHYSLF LQTLLSSSGA PASGQLPSAS
560 570 580 590 600
MRLKFLRLHG GMEQEERTAV FQEFSHSRRG VLLCTDVAAR GLDLPQVTWI
610 620 630 640 650
VQYNAPSSPA EYIHRIGRTA RIGCHGSSLL ILAPSEAEYV NSLASHKINV
660 670 680 690 700
SEIKMEDILC VLTRDDCFKG KRWGAQKSHA VGPQEIRERA TVLQTVFEDY
710 720 730 740 750
VHSSERRVSW AKKALQSFIQ AYATYPRELK HIFHVRSLHL GHVAKSFGLR
760 770 780 790 800
DAPRNLSALT RKKRKAHVKR PDLHKKTQSK HSLAEILRSE YSSGMEADIA
810 820 830 840 850
KVKKQNAPGE PGGRPLQHSL QPTPCFGRGK TLKWRKTQKG VQRDSKTSQK

V
Length:851
Mass (Da):94,087
Last modified:July 19, 2005 - v2
Checksum:iE3C73701FF2F37E7
GO
Isoform 2 (identifier: Q9H8H2-2) [UniParc]FASTAAdd to basket
Also known as: Helicain C

The sequence of this isoform differs from the canonical sequence as follows:
     603-714: YNAPSSPAEY...ERRVSWAKKA → NTSTGLEEPP...STRWLLTKST

Note: No experimental confirmation available.
Show »
Length:778
Mass (Da):85,816
Checksum:i0951A29BEA4E08AF
GO
Isoform 3 (identifier: Q9H8H2-3) [UniParc]FASTAAdd to basket
Also known as: Helicain A

The sequence of this isoform differs from the canonical sequence as follows:
     302-319: RSDGPYALVLVPTRELAL → VLLLSTFYEEEQRLRKVK
     320-851: Missing.

Show »
Length:319
Mass (Da):34,611
Checksum:iC8A423CE304152C6
GO
Isoform 4 (identifier: Q9H8H2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     586-851: Missing.

Note: No experimental confirmation available.
Show »
Length:585
Mass (Da):64,137
Checksum:i2353C61B5C2E5AE2
GO

Sequence cautioni

The sequence AAQ14889 differs from that shown. Reason: Frameshift at positions 816 and 825.Curated
The sequence AAQ14890 differs from that shown. Reason: Frameshift at position 816.Curated
The sequence BAB14644 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB15620 differs from that shown. Reason: Frameshift at position 141.Curated
The sequence CAH70532 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI16565 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti206K → L in BAB15620 (PubMed:14702039).Curated1
Sequence conflicti406L → P in BAB15620 (PubMed:14702039).Curated1
Sequence conflicti527Y → C in BAB15620 (PubMed:14702039).Curated1
Sequence conflicti562M → K in BAB55146 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052164153E → K.Corresponds to variant rs17402080dbSNPEnsembl.1
Natural variantiVAR_052165687R → Q.Corresponds to variant rs34246652dbSNPEnsembl.1
Natural variantiVAR_023065799I → V.3 PublicationsCorresponds to variant rs306547dbSNPEnsembl.1
Natural variantiVAR_023066843R → Q.Corresponds to variant rs306548dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014787302 – 319RSDGP…RELAL → VLLLSTFYEEEQRLRKVK in isoform 3. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_014788320 – 851Missing in isoform 3. 1 PublicationAdd BLAST532
Alternative sequenceiVSP_014789586 – 851Missing in isoform 4. 1 PublicationAdd BLAST266
Alternative sequenceiVSP_014790603 – 714YNAPS…WAKKA → NTSTGLEEPPGLAAMGAACS FWLLRRQNMSTRWLLTKST in isoform 2. 1 PublicationAdd BLAST112

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF427339 mRNA. Translation: AAL26549.1.
AF335567 mRNA. Translation: AAQ14888.1.
AF335568 mRNA. Translation: AAQ14889.1. Frameshift.
AF335569 mRNA. Translation: AAQ14890.1. Frameshift.
AK023695 mRNA. Translation: BAB14644.1. Different initiation.
AK027002 mRNA. Translation: BAB15620.1. Frameshift.
AK027484 mRNA. Translation: BAB55146.1.
AL160165 Genomic DNA. Translation: CAH70530.1.
AL160165, AL354735 Genomic DNA. Translation: CAH70531.1.
AL160165, AL354735 Genomic DNA. Translation: CAH70532.1. Sequence problems.
AL354735, AL160165 Genomic DNA. Translation: CAI16564.1.
AL354735, AL160165 Genomic DNA. Translation: CAI16565.1. Sequence problems.
BC012726 mRNA. Translation: AAH12726.2.
CCDSiCCDS6951.1. [Q9H8H2-1]
CCDS6952.1. [Q9H8H2-4]
CCDS83433.1. [Q9H8H2-3]
RefSeqiNP_001309269.1. NM_001322340.1. [Q9H8H2-2]
NP_001309273.1. NM_001322344.1. [Q9H8H2-3]
NP_073616.6. NM_022779.8. [Q9H8H2-1]
NP_619526.1. NM_138620.1. [Q9H8H2-4]
UniGeneiHs.660767.

Genome annotation databases

EnsembliENST00000310532; ENSP00000310539; ENSG00000125485. [Q9H8H2-4]
ENST00000372153; ENSP00000361226; ENSG00000125485. [Q9H8H2-1]
ENST00000372159; ENSP00000361232; ENSG00000125485. [Q9H8H2-1]
ENST00000480876; ENSP00000479697; ENSG00000125485. [Q9H8H2-3]
GeneIDi64794.
KEGGihsa:64794.
UCSCiuc004cbq.1. human. [Q9H8H2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF427339 mRNA. Translation: AAL26549.1.
AF335567 mRNA. Translation: AAQ14888.1.
AF335568 mRNA. Translation: AAQ14889.1. Frameshift.
AF335569 mRNA. Translation: AAQ14890.1. Frameshift.
AK023695 mRNA. Translation: BAB14644.1. Different initiation.
AK027002 mRNA. Translation: BAB15620.1. Frameshift.
AK027484 mRNA. Translation: BAB55146.1.
AL160165 Genomic DNA. Translation: CAH70530.1.
AL160165, AL354735 Genomic DNA. Translation: CAH70531.1.
AL160165, AL354735 Genomic DNA. Translation: CAH70532.1. Sequence problems.
AL354735, AL160165 Genomic DNA. Translation: CAI16564.1.
AL354735, AL160165 Genomic DNA. Translation: CAI16565.1. Sequence problems.
BC012726 mRNA. Translation: AAH12726.2.
CCDSiCCDS6951.1. [Q9H8H2-1]
CCDS6952.1. [Q9H8H2-4]
CCDS83433.1. [Q9H8H2-3]
RefSeqiNP_001309269.1. NM_001322340.1. [Q9H8H2-2]
NP_001309273.1. NM_001322344.1. [Q9H8H2-3]
NP_073616.6. NM_022779.8. [Q9H8H2-1]
NP_619526.1. NM_138620.1. [Q9H8H2-4]
UniGeneiHs.660767.

3D structure databases

ProteinModelPortaliQ9H8H2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122302. 105 interactors.
IntActiQ9H8H2. 5 interactors.
MINTiMINT-3056737.
STRINGi9606.ENSP00000361232.

PTM databases

iPTMnetiQ9H8H2.
PhosphoSitePlusiQ9H8H2.

Polymorphism and mutation databases

BioMutaiDDX31.
DMDMi71153334.

2D gel databases

SWISS-2DPAGEQ9H8H2.

Proteomic databases

EPDiQ9H8H2.
MaxQBiQ9H8H2.
PaxDbiQ9H8H2.
PeptideAtlasiQ9H8H2.
PRIDEiQ9H8H2.

Protocols and materials databases

DNASUi64794.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310532; ENSP00000310539; ENSG00000125485. [Q9H8H2-4]
ENST00000372153; ENSP00000361226; ENSG00000125485. [Q9H8H2-1]
ENST00000372159; ENSP00000361232; ENSG00000125485. [Q9H8H2-1]
ENST00000480876; ENSP00000479697; ENSG00000125485. [Q9H8H2-3]
GeneIDi64794.
KEGGihsa:64794.
UCSCiuc004cbq.1. human. [Q9H8H2-1]

Organism-specific databases

CTDi64794.
DisGeNETi64794.
GeneCardsiDDX31.
HGNCiHGNC:16715. DDX31.
HPAiHPA020891.
MIMi616533. gene.
neXtProtiNX_Q9H8H2.
OpenTargetsiENSG00000125485.
PharmGKBiPA27218.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0348. Eukaryota.
ENOG410XNT7. LUCA.
GeneTreeiENSGT00550000075041.
HOVERGENiHBG103793.
InParanoidiQ9H8H2.
KOiK14806.
OMAiRDDCFKG.
OrthoDBiEOG091G06RP.
PhylomeDBiQ9H8H2.
TreeFamiTF323273.

Miscellaneous databases

GeneWikiiDDX31.
GenomeRNAii64794.
PROiQ9H8H2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000125485.
CleanExiHS_DDX31.
ExpressionAtlasiQ9H8H2. baseline and differential.
GenevisibleiQ9H8H2. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX31_HUMAN
AccessioniPrimary (citable) accession number: Q9H8H2
Secondary accession number(s): Q5K6N2
, Q5K6N3, Q5K6N4, Q5VZJ4, Q5VZJ9, Q96E91, Q96NY2, Q96SX5, Q9H5K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.