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Q9H832 (UBE2Z_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 Z

EC=6.3.2.19
Alternative name(s):
Uba6-specific E2 conjugating enzyme 1
Short name=Use1
Ubiquitin carrier protein Z
Ubiquitin-protein ligase Z
Gene names
Name:UBE2Z
ORF Names:HOYS7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins By similarity. Specific substrate for UBA6, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation. Ref.1 Ref.10

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Cytoplasm. Nucleus Ref.9.

Tissue specificity

Widely expressed. Highly in placenta, pancreas, spleen and testis. Ref.1 Ref.9

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution

The sequence AAH15890.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB14724.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processApoptosis
Ubl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay. Source: HPA

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H832-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H832-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Ubiquitin-conjugating enzyme E2 Z
PRO_0000280515

Sites

Active site1881Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence1 – 108108Missing in isoform 2.
VSP_023747

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 20, 2007. Version 2.
Checksum: 5AFC148BD8D31356

FASTA35438,210
        10         20         30         40         50         60 
MAESPTEEAA TAGAGAAGPG ASSVAGVVGV SGSGGGFGPP FLPDVWAAAA AAGGAGGPGS 

        70         80         90        100        110        120 
GLAPLPGLPP SAAAHGAALL SHWDPTLSSD WDGERTAPQC LLRIKRDIMS IYKEPPPGMF 

       130        140        150        160        170        180 
VVPDTVDMTK IHALITGPFD TPYEGGFFLF VFRCPPDYPI HPPRVKLMTT GNNTVRFNPN 

       190        200        210        220        230        240 
FYRNGKVCLS ILGTWTGPAW SPAQSISSVL ISIQSLMTEN PYHNEPGFEQ ERHPGDSKNY 

       250        260        270        280        290        300 
NECIRHETIR VAVCDMMEGK CPCPEPLRGV MEKSFLEYYD FYEVACKDRL HLQGQTMQDP 

       310        320        330        340        350 
FGEKRGHFDY QSLLMRLGLI RQKVLERLHN ENAEMDSDSS SSGTETDLHG SLRV 

« Hide

Isoform 2 [UniParc].

Checksum: A0AE68FB9430ACDB
Show »

FASTA24628,075

References

« Hide 'large scale' references
[1]"Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging."
Jin J., Li X., Gygi S.P., Harper J.W.
Nature 447:1135-1138(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Retinoblastoma and Thyroid.
[3]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Skin.
[6]"Molecular cloning of an osteocyte derived gene."
Ikeda A., Turitani K.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-354.
[7]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 109-354.
[8]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 238-354.
Tissue: Brain and Pancreas.
[9]"Cloning and characterization of a gene encoding the human putative ubiquitin conjugating enzyme E2Z (UBE2Z)."
Gu X., Zhao F., Zheng M., Fei X., Chen X., Huang S., Xie Y., Mao Y.
Mol. Biol. Rep. 34:183-188(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]"Identification of novel regulators of apoptosis using a high-throughput cell-based screen."
Park K.M., Kang E., Jeon Y.-J., Kim N., Kim N.-S., Yoo H.-S., Yeom Y.I., Kim S.J.
Mol. Cells 23:170-174(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF623992 mRNA. Translation: ABR25252.1.
AK023917 mRNA. Translation: BAB14724.1. Different initiation.
AK024030 mRNA. Translation: BAB14789.1.
AC091133 Genomic DNA. No translation available.
CH471109 Genomic DNA. Translation: EAW94703.1.
BC015169 mRNA. Translation: AAH15169.2.
BC015890 mRNA. Translation: AAH15890.1. Different initiation.
AB025426 mRNA. Translation: BAB87810.1.
CR457322 mRNA. Translation: CAG33603.1.
AL713782 mRNA. Translation: CAD28542.1.
RefSeqNP_075567.2. NM_023079.4.
UniGeneHs.514297.

3D structure databases

ProteinModelPortalQ9H832.
SMRQ9H832. Positions 93-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122419. 29 interactions.
IntActQ9H832. 30 interactions.
MINTMINT-1388629.
STRING9606.ENSP00000354201.

PTM databases

PhosphoSiteQ9H832.

Polymorphism databases

DMDM134035344.

Proteomic databases

PaxDbQ9H832.
PRIDEQ9H832.

Protocols and materials databases

DNASU65264.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000360943; ENSP00000354201; ENSG00000159202. [Q9H832-1]
GeneID65264.
KEGGhsa:65264.
UCSCuc002ioi.3. human. [Q9H832-1]

Organism-specific databases

CTD65264.
GeneCardsGC17P046985.
HGNCHGNC:25847. UBE2Z.
HPAHPA007922.
MIM611362. gene.
neXtProtNX_Q9H832.
PharmGKBPA142670659.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233451.
HOVERGENHBG083204.
InParanoidQ9H832.
KOK10585.
OMAAVMANMS.
OrthoDBEOG7MSMP5.
PhylomeDBQ9H832.
TreeFamTF354204.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
SignaLinkQ9H832.
UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ9H832.
BgeeQ9H832.
CleanExHS_UBE2Z.
GenevestigatorQ9H832.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi65264.
NextBio67390.
PROQ9H832.
SOURCESearch...

Entry information

Entry nameUBE2Z_HUMAN
AccessionPrimary (citable) accession number: Q9H832
Secondary accession number(s): A6N8M6 expand/collapse secondary AC list , A6NC60, Q7L354, Q8TCM4, Q9H893
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM