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Protein

Ubiquitin-conjugating enzyme E2 Z

Gene

UBE2Z

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Specific substrate for UBA6, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation.2 PublicationsPROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. small conjugating protein transferase activity Source: GO_Central
  4. ubiquitin protein ligase activity Source: GO_Central
  5. ubiquitin protein ligase binding Source: GO_Central

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. protein polyubiquitination Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ9H832.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 Z (EC:6.3.2.19)
Alternative name(s):
Uba6-specific E2 conjugating enzyme 1
Short name:
Use1
Ubiquitin carrier protein Z
Ubiquitin-protein ligase Z
Gene namesi
Name:UBE2Z
ORF Names:HOYS7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:25847. UBE2Z.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670659.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Ubiquitin-conjugating enzyme E2 ZPRO_0000280515Add
BLAST

Proteomic databases

MaxQBiQ9H832.
PaxDbiQ9H832.
PRIDEiQ9H832.

PTM databases

PhosphoSiteiQ9H832.

Expressioni

Tissue specificityi

Widely expressed. Highly in placenta, pancreas, spleen and testis.2 Publications

Gene expression databases

BgeeiQ9H832.
CleanExiHS_UBE2Z.
ExpressionAtlasiQ9H832. baseline and differential.
GenevestigatoriQ9H832.

Organism-specific databases

HPAiHPA007922.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
UBDO152052EBI-720977,EBI-6657186

Protein-protein interaction databases

BioGridi122419. 35 interactions.
IntActiQ9H832. 30 interactions.
MINTiMINT-1388629.
STRINGi9606.ENSP00000354201.

Structurei

3D structure databases

ProteinModelPortaliQ9H832.
SMRiQ9H832. Positions 119-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00540000070023.
HOGENOMiHOG000233451.
HOVERGENiHBG083204.
InParanoidiQ9H832.
KOiK10585.
OMAiAVMANMS.
OrthoDBiEOG7MSMP5.
PhylomeDBiQ9H832.
TreeFamiTF354204.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H832-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESPTEEAA TAGAGAAGPG ASSVAGVVGV SGSGGGFGPP FLPDVWAAAA
60 70 80 90 100
AAGGAGGPGS GLAPLPGLPP SAAAHGAALL SHWDPTLSSD WDGERTAPQC
110 120 130 140 150
LLRIKRDIMS IYKEPPPGMF VVPDTVDMTK IHALITGPFD TPYEGGFFLF
160 170 180 190 200
VFRCPPDYPI HPPRVKLMTT GNNTVRFNPN FYRNGKVCLS ILGTWTGPAW
210 220 230 240 250
SPAQSISSVL ISIQSLMTEN PYHNEPGFEQ ERHPGDSKNY NECIRHETIR
260 270 280 290 300
VAVCDMMEGK CPCPEPLRGV MEKSFLEYYD FYEVACKDRL HLQGQTMQDP
310 320 330 340 350
FGEKRGHFDY QSLLMRLGLI RQKVLERLHN ENAEMDSDSS SSGTETDLHG

SLRV
Length:354
Mass (Da):38,210
Last modified:March 20, 2007 - v2
Checksum:i5AFC148BD8D31356
GO
Isoform 2 (identifier: Q9H832-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Note: No experimental confirmation available.

Show »
Length:246
Mass (Da):28,075
Checksum:iA0AE68FB9430ACDB
GO

Sequence cautioni

The sequence AAH15890.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB14724.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 108108Missing in isoform 2. 1 PublicationVSP_023747Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF623992 mRNA. Translation: ABR25252.1.
AK023917 mRNA. Translation: BAB14724.1. Different initiation.
AK024030 mRNA. Translation: BAB14789.1.
AC091133 Genomic DNA. No translation available.
CH471109 Genomic DNA. Translation: EAW94703.1.
BC015169 mRNA. Translation: AAH15169.2.
BC015890 mRNA. Translation: AAH15890.1. Different initiation.
AB025426 mRNA. Translation: BAB87810.1.
CR457322 mRNA. Translation: CAG33603.1.
AL713782 mRNA. Translation: CAD28542.1.
CCDSiCCDS11540.2. [Q9H832-1]
RefSeqiNP_075567.2. NM_023079.4. [Q9H832-1]
UniGeneiHs.514297.

Genome annotation databases

EnsembliENST00000360943; ENSP00000354201; ENSG00000159202. [Q9H832-1]
GeneIDi65264.
KEGGihsa:65264.
UCSCiuc002ioi.3. human. [Q9H832-1]

Polymorphism databases

DMDMi134035344.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF623992 mRNA. Translation: ABR25252.1.
AK023917 mRNA. Translation: BAB14724.1. Different initiation.
AK024030 mRNA. Translation: BAB14789.1.
AC091133 Genomic DNA. No translation available.
CH471109 Genomic DNA. Translation: EAW94703.1.
BC015169 mRNA. Translation: AAH15169.2.
BC015890 mRNA. Translation: AAH15890.1. Different initiation.
AB025426 mRNA. Translation: BAB87810.1.
CR457322 mRNA. Translation: CAG33603.1.
AL713782 mRNA. Translation: CAD28542.1.
CCDSiCCDS11540.2. [Q9H832-1]
RefSeqiNP_075567.2. NM_023079.4. [Q9H832-1]
UniGeneiHs.514297.

3D structure databases

ProteinModelPortaliQ9H832.
SMRiQ9H832. Positions 119-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122419. 35 interactions.
IntActiQ9H832. 30 interactions.
MINTiMINT-1388629.
STRINGi9606.ENSP00000354201.

PTM databases

PhosphoSiteiQ9H832.

Polymorphism databases

DMDMi134035344.

Proteomic databases

MaxQBiQ9H832.
PaxDbiQ9H832.
PRIDEiQ9H832.

Protocols and materials databases

DNASUi65264.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360943; ENSP00000354201; ENSG00000159202. [Q9H832-1]
GeneIDi65264.
KEGGihsa:65264.
UCSCiuc002ioi.3. human. [Q9H832-1]

Organism-specific databases

CTDi65264.
GeneCardsiGC17P046985.
HGNCiHGNC:25847. UBE2Z.
HPAiHPA007922.
MIMi611362. gene.
neXtProtiNX_Q9H832.
PharmGKBiPA142670659.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00540000070023.
HOGENOMiHOG000233451.
HOVERGENiHBG083204.
InParanoidiQ9H832.
KOiK10585.
OMAiAVMANMS.
OrthoDBiEOG7MSMP5.
PhylomeDBiQ9H832.
TreeFamiTF354204.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ9H832.

Miscellaneous databases

ChiTaRSiUBE2Z. human.
GenomeRNAii65264.
NextBioi67390.
PROiQ9H832.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H832.
CleanExiHS_UBE2Z.
ExpressionAtlasiQ9H832. baseline and differential.
GenevestigatoriQ9H832.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging."
    Jin J., Li X., Gygi S.P., Harper J.W.
    Nature 447:1135-1138(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Retinoblastoma and Thyroid.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  6. "Molecular cloning of an osteocyte derived gene."
    Ikeda A., Turitani K.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-354.
  7. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 109-354.
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 238-354.
    Tissue: Brain and Pancreas.
  9. "Cloning and characterization of a gene encoding the human putative ubiquitin conjugating enzyme E2Z (UBE2Z)."
    Gu X., Zhao F., Zheng M., Fei X., Chen X., Huang S., Xie Y., Mao Y.
    Mol. Biol. Rep. 34:183-188(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  10. "Identification of novel regulators of apoptosis using a high-throughput cell-based screen."
    Park K.M., Kang E., Jeon Y.-J., Kim N., Kim N.-S., Yoo H.-S., Yeom Y.I., Kim S.J.
    Mol. Cells 23:170-174(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBE2Z_HUMAN
AccessioniPrimary (citable) accession number: Q9H832
Secondary accession number(s): A6N8M6
, A6NC60, Q7L354, Q8TCM4, Q9H893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: March 4, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.