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Protein

Ubiquitin-conjugating enzyme E2 Z

Gene

UBE2Z

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Specific substrate for UBA6, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation.PROSITE-ProRule annotation2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ9H832.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 Z (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme Z
Uba6-specific E2 conjugating enzyme 1
Short name:
Use1
Ubiquitin carrier protein Z
Ubiquitin-protein ligase Z
Gene namesi
Name:UBE2Z
ORF Names:HOYS7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:25847. UBE2Z.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670659.

Polymorphism and mutation databases

BioMutaiUBE2Z.
DMDMi134035344.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Ubiquitin-conjugating enzyme E2 ZPRO_0000280515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei337 – 3371PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H832.
MaxQBiQ9H832.
PaxDbiQ9H832.
PRIDEiQ9H832.

PTM databases

iPTMnetiQ9H832.
PhosphoSiteiQ9H832.

Expressioni

Tissue specificityi

Widely expressed. Highly in placenta, pancreas, spleen and testis.2 Publications

Gene expression databases

BgeeiQ9H832.
CleanExiHS_UBE2Z.
ExpressionAtlasiQ9H832. baseline and differential.
GenevisibleiQ9H832. HS.

Organism-specific databases

HPAiHPA007922.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AK8Q96MA63EBI-720977,EBI-8466265
HMBOX1Q6NT763EBI-720977,EBI-2549423
RELQ048643EBI-720977,EBI-307352
UBDO152052EBI-720977,EBI-6657186

GO - Molecular functioni

Protein-protein interaction databases

BioGridi122419. 34 interactions.
IntActiQ9H832. 33 interactions.
MINTiMINT-1388629.
STRINGi9606.ENSP00000354201.

Structurei

Secondary structure

1
354
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi102 – 11312Combined sources
Beta strandi119 – 1235Combined sources
Beta strandi130 – 1367Combined sources
Turni142 – 1454Combined sources
Beta strandi147 – 1537Combined sources
Turni156 – 1594Combined sources
Beta strandi164 – 1674Combined sources
Turni171 – 1744Combined sources
Beta strandi185 – 1873Combined sources
Helixi190 – 1923Combined sources
Beta strandi194 – 1985Combined sources
Helixi206 – 21611Combined sources
Helixi221 – 2244Combined sources
Turni226 – 2294Combined sources
Helixi236 – 25116Combined sources
Helixi254 – 2574Combined sources
Helixi265 – 27713Combined sources
Helixi279 – 2879Combined sources
Helixi288 – 2925Combined sources
Helixi310 – 32516Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5A4PX-ray2.10A1-354[»]
ProteinModelPortaliQ9H832.
SMRiQ9H832. Positions 78-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410ITJE. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00730000110680.
HOGENOMiHOG000233451.
HOVERGENiHBG083204.
InParanoidiQ9H832.
KOiK10585.
OMAiQHETIRV.
OrthoDBiEOG7MSMP5.
PhylomeDBiQ9H832.
TreeFamiTF354204.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H832-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESPTEEAA TAGAGAAGPG ASSVAGVVGV SGSGGGFGPP FLPDVWAAAA
60 70 80 90 100
AAGGAGGPGS GLAPLPGLPP SAAAHGAALL SHWDPTLSSD WDGERTAPQC
110 120 130 140 150
LLRIKRDIMS IYKEPPPGMF VVPDTVDMTK IHALITGPFD TPYEGGFFLF
160 170 180 190 200
VFRCPPDYPI HPPRVKLMTT GNNTVRFNPN FYRNGKVCLS ILGTWTGPAW
210 220 230 240 250
SPAQSISSVL ISIQSLMTEN PYHNEPGFEQ ERHPGDSKNY NECIRHETIR
260 270 280 290 300
VAVCDMMEGK CPCPEPLRGV MEKSFLEYYD FYEVACKDRL HLQGQTMQDP
310 320 330 340 350
FGEKRGHFDY QSLLMRLGLI RQKVLERLHN ENAEMDSDSS SSGTETDLHG

SLRV
Length:354
Mass (Da):38,210
Last modified:March 20, 2007 - v2
Checksum:i5AFC148BD8D31356
GO
Isoform 2 (identifier: Q9H832-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Note: No experimental confirmation available.
Show »
Length:246
Mass (Da):28,075
Checksum:iA0AE68FB9430ACDB
GO

Sequence cautioni

The sequence AAH15890.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB14724.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 108108Missing in isoform 2. 1 PublicationVSP_023747Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF623992 mRNA. Translation: ABR25252.1.
AK023917 mRNA. Translation: BAB14724.1. Different initiation.
AK024030 mRNA. Translation: BAB14789.1.
AC091133 Genomic DNA. No translation available.
CH471109 Genomic DNA. Translation: EAW94703.1.
BC015169 mRNA. Translation: AAH15169.2.
BC015890 mRNA. Translation: AAH15890.1. Different initiation.
AB025426 mRNA. Translation: BAB87810.1.
CR457322 mRNA. Translation: CAG33603.1.
AL713782 mRNA. Translation: CAD28542.1.
CCDSiCCDS11540.2. [Q9H832-1]
RefSeqiNP_075567.2. NM_023079.4. [Q9H832-1]
UniGeneiHs.514297.

Genome annotation databases

EnsembliENST00000360943; ENSP00000354201; ENSG00000159202. [Q9H832-1]
GeneIDi65264.
KEGGihsa:65264.
UCSCiuc002ioi.4. human. [Q9H832-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF623992 mRNA. Translation: ABR25252.1.
AK023917 mRNA. Translation: BAB14724.1. Different initiation.
AK024030 mRNA. Translation: BAB14789.1.
AC091133 Genomic DNA. No translation available.
CH471109 Genomic DNA. Translation: EAW94703.1.
BC015169 mRNA. Translation: AAH15169.2.
BC015890 mRNA. Translation: AAH15890.1. Different initiation.
AB025426 mRNA. Translation: BAB87810.1.
CR457322 mRNA. Translation: CAG33603.1.
AL713782 mRNA. Translation: CAD28542.1.
CCDSiCCDS11540.2. [Q9H832-1]
RefSeqiNP_075567.2. NM_023079.4. [Q9H832-1]
UniGeneiHs.514297.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5A4PX-ray2.10A1-354[»]
ProteinModelPortaliQ9H832.
SMRiQ9H832. Positions 78-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122419. 34 interactions.
IntActiQ9H832. 33 interactions.
MINTiMINT-1388629.
STRINGi9606.ENSP00000354201.

PTM databases

iPTMnetiQ9H832.
PhosphoSiteiQ9H832.

Polymorphism and mutation databases

BioMutaiUBE2Z.
DMDMi134035344.

Proteomic databases

EPDiQ9H832.
MaxQBiQ9H832.
PaxDbiQ9H832.
PRIDEiQ9H832.

Protocols and materials databases

DNASUi65264.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360943; ENSP00000354201; ENSG00000159202. [Q9H832-1]
GeneIDi65264.
KEGGihsa:65264.
UCSCiuc002ioi.4. human. [Q9H832-1]

Organism-specific databases

CTDi65264.
GeneCardsiUBE2Z.
HGNCiHGNC:25847. UBE2Z.
HPAiHPA007922.
MIMi611362. gene.
neXtProtiNX_Q9H832.
PharmGKBiPA142670659.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITJE. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00730000110680.
HOGENOMiHOG000233451.
HOVERGENiHBG083204.
InParanoidiQ9H832.
KOiK10585.
OMAiQHETIRV.
OrthoDBiEOG7MSMP5.
PhylomeDBiQ9H832.
TreeFamiTF354204.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ9H832.

Miscellaneous databases

ChiTaRSiUBE2Z. human.
GenomeRNAii65264.
NextBioi67390.
PROiQ9H832.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H832.
CleanExiHS_UBE2Z.
ExpressionAtlasiQ9H832. baseline and differential.
GenevisibleiQ9H832. HS.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging."
    Jin J., Li X., Gygi S.P., Harper J.W.
    Nature 447:1135-1138(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Retinoblastoma and Thyroid.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  6. "Molecular cloning of an osteocyte derived gene."
    Ikeda A., Turitani K.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-354.
  7. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 109-354.
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 238-354.
    Tissue: Brain and Pancreas.
  9. "Cloning and characterization of a gene encoding the human putative ubiquitin conjugating enzyme E2Z (UBE2Z)."
    Gu X., Zhao F., Zheng M., Fei X., Chen X., Huang S., Xie Y., Mao Y.
    Mol. Biol. Rep. 34:183-188(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  10. "Identification of novel regulators of apoptosis using a high-throughput cell-based screen."
    Park K.M., Kang E., Jeon Y.-J., Kim N., Kim N.-S., Yoo H.-S., Yeom Y.I., Kim S.J.
    Mol. Cells 23:170-174(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBE2Z_HUMAN
AccessioniPrimary (citable) accession number: Q9H832
Secondary accession number(s): A6N8M6
, A6NC60, Q7L354, Q8TCM4, Q9H893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: March 16, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.