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Q9H7Z7

- PGES2_HUMAN

UniProt

Q9H7Z7 - PGES2_HUMAN

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Protein

Prostaglandin E synthase 2

Gene
PTGES2, C9orf15, PGES2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Dual function enzyme which exhibits both isomerase and lyase activities. Its catalytic activity is altered by the presence or absence of the cofactors glutathione (GSH) and heme. The GSH-heme complex-bound enzyme (mPGES-2h) acts as a lyase and catalyzes the degradation of prostaglandin E2 H2 (PGH2) to 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid (HHT) and malondialdehyde (MDA) while the GSH-heme complex-free enzyme (mPGES-2) acts as an isomerase and catalyzes the isomerization of PGH2 into the more stable prostaglandin E2 (PGE2) form By similarity.

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate.
(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = malonaldehyde + (5Z,8E,10E)-(12S)-hydroxyheptadeca-5,8,10-trienoate.

Cofactori

Dihydrolipoic acid; required for isomerase activity.1 Publication
Glutathione (GSH). Required for lyase activity By similarity.1 Publication
Heme. Required for lyase activity By similarity.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei87 – 882Cleavage By similarity
Binding sitei148 – 1481Glutathione; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. DNA binding Source: Ensembl
  2. electron carrier activity Source: InterPro
  3. glutathione binding Source: UniProtKB
  4. heme binding Source: UniProtKB
  5. lyase activity Source: UniProtKB
  6. prostaglandin-E synthase activity Source: UniProtKB-EC
  7. protein disulfide oxidoreductase activity Source: InterPro

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. positive regulation of transcription, DNA-templated Source: Ensembl
  3. prostaglandin biosynthetic process Source: UniProtKB-UniPathway
  4. secretion Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS07514-MONOMER.
BRENDAi5.3.99.3. 2681.
UniPathwayiUPA00662.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E synthase 2 (EC:4.1.2.-, EC:5.3.99.3)
Alternative name(s):
Microsomal prostaglandin E synthase 2
Short name:
mPGES-2
Cleaved into the following chain:
Gene namesi
Name:PTGES2
Synonyms:C9orf15, PGES2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:17822. PTGES2.

Subcellular locationi

Golgi apparatus membrane; Single-pass membrane protein 1 Publication
Chain Prostaglandin E synthase 2 truncated form : Cytoplasmperinuclear region
Note: Synthesized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form. The truncated form is cytoplasmic and is enriched in the perinuclear region.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757Lumenal Reviewed predictionAdd
BLAST
Transmembranei58 – 7417Helical; Reviewed predictionAdd
BLAST
Topological domaini75 – 377303Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. mitochondrion Source: LIFEdb
  5. nucleus Source: Ensembl
  6. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi110 – 1101C → S: Loss of function. 1 Publication
Mutagenesisi113 – 1131C → S: Does not strongly affect enzyme activity. 1 Publication

Organism-specific databases

PharmGKBiPA33949.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Prostaglandin E synthase 2PRO_0000013127Add
BLAST
Chaini88 – 377290Prostaglandin E synthase 2 truncated form By similarityPRO_0000013128Add
BLAST

Proteomic databases

MaxQBiQ9H7Z7.
PaxDbiQ9H7Z7.
PRIDEiQ9H7Z7.

PTM databases

PhosphoSiteiQ9H7Z7.

Expressioni

Tissue specificityi

Widely expressed. Expressed in the heart, including apex, inter-ventricular septum, both atria and ventricles, but not in the aorta. Also expressed in fetal heart. Detected in various regions of the brain: cerebellum; occipital, frontal and parietal lobes. Also expressed in the lymph nodes, skeletal muscle, kidney and trachea, but not in the thymus or lung. Overexpressed in colorectal cancer.1 Publication

Gene expression databases

ArrayExpressiQ9H7Z7.
BgeeiQ9H7Z7.
CleanExiHS_PTGES2.
GenevestigatoriQ9H7Z7.

Organism-specific databases

HPAiHPA020631.

Interactioni

Subunit structurei

Homodimer. May interact with CEBPB By similarity. Interacts with EXOSC10.1 Publication

Protein-protein interaction databases

BioGridi123135. 7 interactions.
IntActiQ9H7Z7. 6 interactions.
STRINGi9606.ENSP00000345341.

Structurei

3D structure databases

ProteinModelPortaliQ9H7Z7.
SMRiQ9H7Z7. Positions 100-373.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 193104GlutaredoxinAdd
BLAST
Domaini263 – 377115GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 1652Glutathione binding By similarity

Sequence similaritiesi

Belongs to the GST superfamily.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG316980.
HOGENOMiHOG000231901.
HOVERGENiHBG069136.
InParanoidiQ9H7Z7.
KOiK05309.
OMAiAMYFISK.
OrthoDBiEOG7060R5.
PhylomeDBiQ9H7Z7.
TreeFamiTF314304.

Family and domain databases

Gene3Di1.20.1050.10. 2 hits.
3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS00195. GLUTAREDOXIN_1. 1 hit.
PS51354. GLUTAREDOXIN_2. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9H7Z7-1 [UniParc]FASTAAdd to Basket

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MDPAARVVRA LWPGGCALAW RLGGRPQPLL PTQSRAGFAG AAGGPSPVAA    50
ARKGSPRLLG AAALALGGAL GLYHTARWHL RAQDLHAERS AAQLSLSSRL 100
QLTLYQYKTC PFCSKVRAFL DFHALPYQVV EVNPVRRAEI KFSSYRKVPI 150
LVAQEGESSQ QLNDSSVIIS ALKTYLVSGQ PLEEIITYYP AMKAVNEQGK 200
EVTEFGNKYW LMLNEKEAQQ VYGGKEARTE EMKWRQWADD WLVHLISPNV 250
YRTPTEALAS FDYIVREGKF GAVEGAVAKY MGAAAMYLIS KRLKSRHRLQ 300
DNVREDLYEA ADKWVAAVGK DRPFMGGQKP NLADLAVYGV LRVMEGLDAF 350
DDLMQHTHIQ PWYLRVERAI TEASPAH 377
Length:377
Mass (Da):41,943
Last modified:March 1, 2001 - v1
Checksum:i82F2DF867BB8337C
GO

Sequence cautioni

The sequence CAI13822.1 differs from that shown. Reason: Erroneous gene model prediction.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti298 – 2981R → H.
Corresponds to variant rs13283456 [ dbSNP | Ensembl ].
VAR_049494

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti319 – 3191G → S in BAD97240. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK024100 mRNA. Translation: BAB14826.1.
AK223520 mRNA. Translation: BAD97240.1.
AL590708 Genomic DNA. Translation: CAI13821.1.
AL590708 Genomic DNA. Translation: CAI13822.1. Sequence problems.
BC009397 mRNA. Translation: AAH09397.2.
BC009456 mRNA. Translation: AAH09456.1.
BC011613 mRNA. Translation: AAH11613.1.
CCDSiCCDS6891.1.
RefSeqiNP_001243264.1. NM_001256335.1.
NP_079348.1. NM_025072.6.
NP_945176.1. NM_198938.2.
UniGeneiHs.495219.

Genome annotation databases

EnsembliENST00000338961; ENSP00000345341; ENSG00000148334.
GeneIDi80142.
KEGGihsa:80142.
UCSCiuc004bti.4. human.

Polymorphism databases

DMDMi73921741.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK024100 mRNA. Translation: BAB14826.1 .
AK223520 mRNA. Translation: BAD97240.1 .
AL590708 Genomic DNA. Translation: CAI13821.1 .
AL590708 Genomic DNA. Translation: CAI13822.1 . Sequence problems.
BC009397 mRNA. Translation: AAH09397.2 .
BC009456 mRNA. Translation: AAH09456.1 .
BC011613 mRNA. Translation: AAH11613.1 .
CCDSi CCDS6891.1.
RefSeqi NP_001243264.1. NM_001256335.1.
NP_079348.1. NM_025072.6.
NP_945176.1. NM_198938.2.
UniGenei Hs.495219.

3D structure databases

ProteinModelPortali Q9H7Z7.
SMRi Q9H7Z7. Positions 100-373.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123135. 7 interactions.
IntActi Q9H7Z7. 6 interactions.
STRINGi 9606.ENSP00000345341.

Chemistry

BindingDBi Q9H7Z7.
ChEMBLi CHEMBL4411.

PTM databases

PhosphoSitei Q9H7Z7.

Polymorphism databases

DMDMi 73921741.

Proteomic databases

MaxQBi Q9H7Z7.
PaxDbi Q9H7Z7.
PRIDEi Q9H7Z7.

Protocols and materials databases

DNASUi 80142.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000338961 ; ENSP00000345341 ; ENSG00000148334 .
GeneIDi 80142.
KEGGi hsa:80142.
UCSCi uc004bti.4. human.

Organism-specific databases

CTDi 80142.
GeneCardsi GC09M130882.
HGNCi HGNC:17822. PTGES2.
HPAi HPA020631.
MIMi 608152. gene.
neXtProti NX_Q9H7Z7.
PharmGKBi PA33949.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG316980.
HOGENOMi HOG000231901.
HOVERGENi HBG069136.
InParanoidi Q9H7Z7.
KOi K05309.
OMAi AMYFISK.
OrthoDBi EOG7060R5.
PhylomeDBi Q9H7Z7.
TreeFami TF314304.

Enzyme and pathway databases

UniPathwayi UPA00662 .
BioCyci MetaCyc:HS07514-MONOMER.
BRENDAi 5.3.99.3. 2681.

Miscellaneous databases

ChiTaRSi PTGES2. human.
GeneWikii PTGES2.
GenomeRNAii 80142.
NextBioi 70401.
PROi Q9H7Z7.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9H7Z7.
Bgeei Q9H7Z7.
CleanExi HS_PTGES2.
Genevestigatori Q9H7Z7.

Family and domain databases

Gene3Di 1.20.1050.10. 2 hits.
3.40.30.10. 1 hit.
InterProi IPR002109. Glutaredoxin.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF14497. GST_C_3. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS00195. GLUTAREDOXIN_1. 1 hit.
PS51354. GLUTAREDOXIN_2. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  2. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Lung.
  5. "Identification and characterization of a novel type of membrane-associated prostaglandin E synthase."
    Tanikawa N., Ohmiya Y., Ohkubo H., Hashimoto K., Kangawa K., Kojima M., Ito S., Watanabe K.
    Biochem. Biophys. Res. Commun. 291:884-889(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Essential 110Cys in active site of membrane-associated prostaglandin E synthase-2."
    Watanabe K., Ohkubo H., Niwa H., Tanikawa N., Koda N., Ito S., Ohmiya Y.
    Biochem. Biophys. Res. Commun. 306:577-581(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, MUTAGENESIS OF CYS-110 AND CYS-113.
  7. "Cellular prostaglandin E2 production by membrane-bound prostaglandin E synthase-2 via both cyclooxygenases-1 and -2."
    Murakami M., Nakashima K., Kamei D., Masuda S., Ishikawa Y., Ishii T., Ohmiya Y., Watanabe K., Kudo I.
    J. Biol. Chem. 278:37937-37947(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE, SUBCELLULAR LOCATION.
  8. "A protein interaction framework for human mRNA degradation."
    Lehner B., Sanderson C.M.
    Genome Res. 14:1315-1323(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EXOSC10.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPGES2_HUMAN
AccessioniPrimary (citable) accession number: Q9H7Z7
Secondary accession number(s): Q53EW9
, Q5SYV6, Q96GI0, Q96GL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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