ID   IFT22_HUMAN             Reviewed;         185 AA.
AC   Q9H7X7; Q49AG1; Q69YV5; Q9BSW4;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 155.
DE   RecName: Full=Intraflagellar transport protein 22 homolog;
DE   AltName: Full=Rab-like protein 5;
GN   Name=IFT22; Synonyms=RABL5;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC   TISSUE=Mammary gland, and Ovary;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA   Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA   Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA   Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA   Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA   Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA   Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA   Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA   Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA   Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA   Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA   Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA   Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA   Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA   Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA   McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA   Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC   TISSUE=Brain, and Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 93-185 (ISOFORMS 1/2/3).
RC   TISSUE=Amygdala;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma, and Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
CC   -!- FUNCTION: Small GTPase-like component of the intraflagellar transport
CC       (IFT) complex B. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the IFT complex B, at least composed of IFT20,
CC       IFT22, IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80,
CC       IFT81, and IFT88. Interacts with IFT88 (By similarity). Interacts with
CC       CFAP61 (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:Q9DAI2}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q9H7X7-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9H7X7-2; Sequence=VSP_021112;
CC       Name=3;
CC         IsoId=Q9H7X7-3; Sequence=VSP_045182;
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC       {ECO:0000305}.
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DR   EMBL; AK023287; BAG51181.1; -; mRNA.
DR   EMBL; AK024179; BAB14846.1; -; mRNA.
DR   EMBL; AK298888; BAH12896.1; -; mRNA.
DR   EMBL; AC006329; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471197; EAW50217.1; -; Genomic_DNA.
DR   EMBL; BC004522; AAH04522.1; -; mRNA.
DR   EMBL; BC009823; AAH09823.1; -; mRNA.
DR   EMBL; BC038668; AAH38668.1; -; mRNA.
DR   EMBL; AL157469; CAH10693.1; -; mRNA.
DR   CCDS; CCDS47670.1; -. [Q9H7X7-2]
DR   CCDS; CCDS47671.1; -. [Q9H7X7-3]
DR   CCDS; CCDS5719.1; -. [Q9H7X7-1]
DR   RefSeq; NP_001124292.1; NM_001130820.2. [Q9H7X7-2]
DR   RefSeq; NP_001124293.1; NM_001130821.2. [Q9H7X7-3]
DR   RefSeq; NP_001124294.1; NM_001130822.2. [Q9H7X7-3]
DR   RefSeq; NP_001274454.1; NM_001287525.1. [Q9H7X7-3]
DR   RefSeq; NP_001274455.1; NM_001287526.1. [Q9H7X7-3]
DR   RefSeq; NP_073614.1; NM_022777.3. [Q9H7X7-1]
DR   AlphaFoldDB; Q9H7X7; -.
DR   SMR; Q9H7X7; -.
DR   BioGRID; 122300; 50.
DR   ComplexPortal; CPX-5022; Intraflagellar transport complex B.
DR   CORUM; Q9H7X7; -.
DR   IntAct; Q9H7X7; 25.
DR   STRING; 9606.ENSP00000320359; -.
DR   iPTMnet; Q9H7X7; -.
DR   PhosphoSitePlus; Q9H7X7; -.
DR   BioMuta; IFT22; -.
DR   DMDM; 74733725; -.
DR   EPD; Q9H7X7; -.
DR   jPOST; Q9H7X7; -.
DR   MassIVE; Q9H7X7; -.
DR   MaxQB; Q9H7X7; -.
DR   PaxDb; 9606-ENSP00000320359; -.
DR   PeptideAtlas; Q9H7X7; -.
DR   ProteomicsDB; 62041; -.
DR   ProteomicsDB; 81155; -. [Q9H7X7-1]
DR   ProteomicsDB; 81156; -. [Q9H7X7-2]
DR   Pumba; Q9H7X7; -.
DR   Antibodypedia; 31006; 88 antibodies from 18 providers.
DR   DNASU; 64792; -.
DR   Ensembl; ENST00000315322.10; ENSP00000320359.4; ENSG00000128581.17. [Q9H7X7-1]
DR   Ensembl; ENST00000422177.5; ENSP00000392087.1; ENSG00000128581.17. [Q9H7X7-1]
DR   Ensembl; ENST00000437644.2; ENSP00000390770.2; ENSG00000128581.17. [Q9H7X7-2]
DR   Ensembl; ENST00000498704.6; ENSP00000429648.1; ENSG00000128581.17. [Q9H7X7-3]
DR   Ensembl; ENST00000517481.5; ENSP00000429202.1; ENSG00000128581.17. [Q9H7X7-3]
DR   Ensembl; ENST00000621899.4; ENSP00000478948.1; ENSG00000128581.17. [Q9H7X7-3]
DR   GeneID; 64792; -.
DR   KEGG; hsa:64792; -.
DR   MANE-Select; ENST00000315322.10; ENSP00000320359.4; NM_022777.4; NP_073614.1.
DR   UCSC; uc003uyl.5; human. [Q9H7X7-1]
DR   AGR; HGNC:21895; -.
DR   CTD; 64792; -.
DR   GeneCards; IFT22; -.
DR   HGNC; HGNC:21895; IFT22.
DR   HPA; ENSG00000128581; Low tissue specificity.
DR   MIM; 620505; gene.
DR   neXtProt; NX_Q9H7X7; -.
DR   OpenTargets; ENSG00000128581; -.
DR   PharmGKB; PA134871769; -.
DR   VEuPathDB; HostDB:ENSG00000128581; -.
DR   eggNOG; ENOG502RXD4; Eukaryota.
DR   GeneTree; ENSGT00390000013187; -.
DR   HOGENOM; CLU_2195962_0_0_1; -.
DR   InParanoid; Q9H7X7; -.
DR   OMA; WYSMFIS; -.
DR   OrthoDB; 1326777at2759; -.
DR   PhylomeDB; Q9H7X7; -.
DR   TreeFam; TF313208; -.
DR   PathwayCommons; Q9H7X7; -.
DR   Reactome; R-HSA-5620924; Intraflagellar transport.
DR   SignaLink; Q9H7X7; -.
DR   BioGRID-ORCS; 64792; 13 hits in 1154 CRISPR screens.
DR   ChiTaRS; IFT22; human.
DR   GenomeRNAi; 64792; -.
DR   Pharos; Q9H7X7; Tbio.
DR   PRO; PR:Q9H7X7; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q9H7X7; Protein.
DR   Bgee; ENSG00000128581; Expressed in bronchial epithelial cell and 192 other cell types or tissues.
DR   ExpressionAtlas; Q9H7X7; baseline and differential.
DR   GO; GO:0005813; C:centrosome; IEA:Ensembl.
DR   GO; GO:0097542; C:ciliary tip; TAS:Reactome.
DR   GO; GO:0005929; C:cilium; TAS:Reactome.
DR   GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR   GO; GO:0030992; C:intraciliary transport particle B; IPI:ComplexPortal.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR   GO; GO:0060271; P:cilium assembly; NAS:ComplexPortal.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0035720; P:intraciliary anterograde transport; NAS:ComplexPortal.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR24073; DRAB5-RELATED; 1.
DR   PANTHER; PTHR24073:SF528; INTRAFLAGELLAR TRANSPORT PROTEIN 22 HOMOLOG; 1.
DR   Pfam; PF08477; Roc; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   Genevisible; Q9H7X7; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell projection; Cilium; GTP-binding;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome.
FT   CHAIN           1..185
FT                   /note="Intraflagellar transport protein 22 homolog"
FT                   /id="PRO_0000253733"
FT   BINDING         10..17
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         63..67
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         123..126
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         137
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   VAR_SEQ         1..77
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:14702039,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_045182"
FT   VAR_SEQ         40..69
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_021112"
SQ   SEQUENCE   185 AA;  20835 MW;  993D2ECEFE14A673 CRC64;
     MLKAKILFVG PCESGKTVLA NFLTESSDIT EYSPTQGVRI LEFENPHVTS NNKGTGCEFE
     LWDCGGDAKF ESCWPALMKD AHGVVIVFNA DIPSHRKEME MWYSCFVQQP SLQDTQCMLI
     AHHKPGSGDD KGSLSLSPPL NKLKLVHSNL EDDPEEIRME FIKYLKSIIN SMSESRDREE
     MSIMT
//