Q9H7L9 (SDS3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sin3 histone deacetylase corepressor complex component SDS3

Alternative name(s):
45 kDa Sin3-associated polypeptide
Suppressor of defective silencing 3 protein homolog

Gene names

Name:	SUDS3
Synonyms:	SAP45, SDS3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways By similarity. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Ref.2 UniProtKB Q8BR65
Subunit structure	Homodimer. Component of the SIN3 histone deacetylase (HDAC) corepressor complex. Interacts with SIN3A. Interaction with SIN3B enhances the interaction between SIN3B and HDAC1 to form a complex By similarity. Interacts with HCFC1. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Ref.5 UniProtKB Q8BR65
Tissue specificity	Expressed in various cancer cell ines. Ref.2
Domain	The C-terminus is involved in transcriptional repression by HDAC-independent mechanisms.
Sequence similarities	Belongs to the SDS3 family.
Sequence caution	The sequence BAB14685.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15750.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Domain	Coiled coil
Molecular function	Chromatin regulator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Sin3 complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	histone deacetylase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRMS1	Q9HCU9	2	EBI-540496,EBI-714781
HCFC1	P51610	2	EBI-540496,EBI-396176

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Sin3 histone deacetylase corepressor complex component SDS3		PRO_0000097652
Regions
	Region	188 – 226	39	Sin3 interaction domain (SID) By similarity UniProtKB Q8BR65
	Coiled coil	66 – 171	106	Potential
Amino acid modifications
	Modified residue	45	1	Phosphoserine Ref.6 Ref.8 Ref.9
	Modified residue	49	1	Phosphothreonine Ref.6 Ref.8 Ref.9
	Modified residue	53	1	Phosphoserine Ref.8 Ref.9
	Modified residue	55	1	Phosphothreonine Ref.6
	Modified residue	234	1	Phosphoserine Ref.6 Ref.8 Ref.10
	Modified residue	236	1	Phosphoserine Ref.7
	Modified residue	237	1	Phosphoserine Ref.8
	Modified residue	244	1	Phosphothreonine Ref.7 Ref.8
Experimental info
	Sequence conflict	164 – 171	8	EKLTMELT → HASAHASA in AAH30252. Ref.2
	Sequence conflict	286	1	S → R in BAB14685. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9H7L9 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 10A7393B607117EB	FASTA	328	38,136
	10 20 30 40 50 60 MSAAGLLAPA PAQAGAPPAP EYYPEEDEEL ESAEDDERSC RGRESDEDTE DASETDLAKH 70 80 90 100 110 120 DEEDYVEMKE QMYQDKLASL KRQLQQLQEG TLQEYQKRMK KLDQQYKERI RNAELFLQLE 130 140 150 160 170 180 TEQVERNYIK EKKAAVKEFE DKKVELKENL IAELEEKKKM IENEKLTMEL TGDSMEVKPI 190 200 210 220 230 240 MTRKLRRRPN DPVPIPDKRR KPAPAQLNYL LTDEQIMEDL RTLNKLKSPK RPASPSSPEH 250 260 270 280 290 300 LPATPAESPA QRFEARIEDG KLYYDKRWYH KSQAIYLESK DNQKLSCVIS SVGANEIWVR 310 320 KTSDSTKMRI YLGQLQRGLF VIRRRSAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of long cDNA clones from human adult spleen." Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O. DNA Res. 7:357-366(2000) [PubMed: 11214971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[2]	"Identification and characterization of three new components of the mSin3A corepressor complex." Fleischer T.C., Yun U.J., Ayer D.E. Mol. Cell. Biol. 23:3456-3467(2003) [PubMed: 12724404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION IN A MSIN3A COREPRESSOR COMPLEX WITH SIN3A; SAP130; SUDS3; ARID4B; HDAC1 AND HDAC2.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Placenta.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 130-328. Tissue: Placenta and Spleen.
[5]	"Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1." Wysocka J., Myers M.P., Laherty C.D., Eisenman R.N., Herr W. Genes Dev. 17:896-911(2003) [PubMed: 12670868] [Abstract] Cited for: INTERACTION WITH HCFC1.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49; THR-55 AND SER-234, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236 AND THR-244, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49; SER-53; SER-234; SER-237 AND THR-244, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49 AND SER-53, MASS SPECTROMETRY. Tissue: Liver.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK024460 mRNA. Translation: BAB15750.1. Different initiation. BC030252 mRNA. Translation: AAH30252.1. BC098404 mRNA. Translation: AAH98404.1. AK023801 mRNA. Translation: BAB14685.1. Different initiation.
IPI	IPI00607645.
RefSeq	NP_071936.2. NM_022491.2.
UniGene	Hs.416630. Hs.602312.
3D structure databases
ProteinModelPortal	Q9H7L9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H7L9. 3 interactions.
STRING	Q9H7L9.
PTM databases
PhosphoSite	Q9H7L9.
Polymorphism databases
DMDM	68053233.
Proteomic databases
PRIDE	Q9H7L9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000397564; ENSP00000380695; ENSG00000111707.
GeneID	64426.
KEGG	hsa:64426.
UCSC	uc001twz.1. human.
Organism-specific databases
CTD	64426.
GeneCards	GC12P118814.
H-InvDB	HIX0011049.
HGNC	HGNC:29545. SUDS3.
MIM	608250. gene.
neXtProt	NX_Q9H7L9.
PharmGKB	PA143485626.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07799.
GeneTree	ENSGT00530000063177.
HOGENOM	HBG446382.
HOVERGEN	HBG108465.
InParanoid	Q9H7L9.
OMA	VEMKEQM.
OrthoDB	EOG4N8R5F.
PhylomeDB	Q9H7L9.
Gene expression databases
ArrayExpress	Q9H7L9.
Bgee	Q9H7L9.
CleanEx	HS_SUDS3.
Genevestigator	Q9H7L9.
GermOnline	ENSG00000111707. Homo sapiens.
Family and domain databases
InterPro	IPR013907. Sds3. [Graphical view]
Pfam	PF08598. Sds3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	66429.
SOURCE	Search...

Entry information

Entry name

SDS3_HUMAN

Accession

Primary (citable) accession number: Q9H7L9
Secondary accession number(s): Q4KMQ5, Q8N6H0, Q9H8D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.