Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sin3 histone deacetylase corepressor complex component SDS3

Gene

SUDS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways through regulation of apoptosis. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.2 Publications

GO - Molecular functioni

  1. enzyme binding Source: UniProtKB
  2. histone deacetylase binding Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. histone deacetylation Source: UniProtKB
  3. negative regulation of transcription, DNA-templated Source: UniProtKB
  4. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  5. positive regulation of apoptotic process Source: UniProtKB
  6. substantia nigra development Source: UniProtKB
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_200856. NoRC negatively regulates rRNA expression.
REACT_228222. HDACs deacetylate histones.

Names & Taxonomyi

Protein namesi
Recommended name:
Sin3 histone deacetylase corepressor complex component SDS3
Alternative name(s):
45 kDa Sin3-associated polypeptide
Suppressor of defective silencing 3 protein homolog
Gene namesi
Name:SUDS3
Synonyms:SAP45, SDS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:29545. SUDS3.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. nucleus Source: UniProtKB
  3. Sin3 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485626.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 328327Sin3 histone deacetylase corepressor complex component SDS3PRO_0000097652Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei32 – 321Phosphoserine1 Publication
Modified residuei45 – 451Phosphoserine5 Publications
Modified residuei49 – 491Phosphothreonine4 Publications
Modified residuei53 – 531Phosphoserine1 Publication
Modified residuei228 – 2281Phosphoserine1 Publication
Modified residuei234 – 2341Phosphoserine4 Publications
Modified residuei237 – 2371PhosphoserineBy similarity
Modified residuei244 – 2441Phosphothreonine1 Publication

Post-translational modificationi

Polyubiquitinated. 'Lys-63'-polyubiquitinated SUDS3 positively regulates histone deacetylation. Regulated through deubiquitination by USP17L2/USP17 that cleaves 'Lys-63'-linked ubiquitin chains.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9H7L9.
PaxDbiQ9H7L9.
PRIDEiQ9H7L9.

PTM databases

PhosphoSiteiQ9H7L9.

Expressioni

Tissue specificityi

Expressed in various cancer cell ines.1 Publication

Gene expression databases

BgeeiQ9H7L9.
CleanExiHS_SUDS3.
ExpressionAtlasiQ9H7L9. baseline and differential.
GenevestigatoriQ9H7L9.

Organism-specific databases

HPAiHPA040402.
HPA041972.

Interactioni

Subunit structurei

Homodimer. Component of the SIN3 histone deacetylase (HDAC) corepressor complex. Interacts with SIN3A. Interaction with SIN3B enhances the interaction between SIN3B and HDAC1 to form a complex (By similarity). Interacts with HCFC1. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with USP17L2; the interaction is direct.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BRMS1Q9HCU92EBI-540496,EBI-714781
HCFC1P516102EBI-540496,EBI-396176

Protein-protein interaction databases

BioGridi122174. 21 interactions.
IntActiQ9H7L9. 8 interactions.
STRINGi9606.ENSP00000380695.

Structurei

3D structure databases

ProteinModelPortaliQ9H7L9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 170169Mediates interaction with USP17L2Add
BLAST
Regioni188 – 22639Sin3 interaction domain (SID)By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili66 – 171106Sequence AnalysisAdd
BLAST

Domaini

The C-terminus is involved in transcriptional repression by HDAC-independent mechanisms.

Sequence similaritiesi

Belongs to the SDS3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG311652.
GeneTreeiENSGT00530000063177.
HOGENOMiHOG000007054.
HOVERGENiHBG108465.
InParanoidiQ9H7L9.
OrthoDBiEOG7VQJFB.
PhylomeDBiQ9H7L9.
TreeFamiTF323740.

Family and domain databases

InterProiIPR013907. Sds3.
[Graphical view]
PfamiPF08598. Sds3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9H7L9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAAGLLAPA PAQAGAPPAP EYYPEEDEEL ESAEDDERSC RGRESDEDTE
60 70 80 90 100
DASETDLAKH DEEDYVEMKE QMYQDKLASL KRQLQQLQEG TLQEYQKRMK
110 120 130 140 150
KLDQQYKERI RNAELFLQLE TEQVERNYIK EKKAAVKEFE DKKVELKENL
160 170 180 190 200
IAELEEKKKM IENEKLTMEL TGDSMEVKPI MTRKLRRRPN DPVPIPDKRR
210 220 230 240 250
KPAPAQLNYL LTDEQIMEDL RTLNKLKSPK RPASPSSPEH LPATPAESPA
260 270 280 290 300
QRFEARIEDG KLYYDKRWYH KSQAIYLESK DNQKLSCVIS SVGANEIWVR
310 320
KTSDSTKMRI YLGQLQRGLF VIRRRSAA
Length:328
Mass (Da):38,136
Last modified:June 21, 2005 - v2
Checksum:i10A7393B607117EB
GO

Sequence cautioni

The sequence BAB14685.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB15750.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1718EKLTMELT → HASAHASA in AAH30252. (PubMed:12724404)Curated
Sequence conflicti286 – 2861S → R in BAB14685. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024460 mRNA. Translation: BAB15750.1. Different initiation.
BC030252 mRNA. Translation: AAH30252.1.
BC098404 mRNA. Translation: AAH98404.1.
AK023801 mRNA. Translation: BAB14685.1. Different initiation.
CCDSiCCDS44993.1.
RefSeqiNP_071936.2. NM_022491.2.
UniGeneiHs.416630.
Hs.602312.

Genome annotation databases

EnsembliENST00000543473; ENSP00000443988; ENSG00000111707.
GeneIDi64426.
KEGGihsa:64426.
UCSCiuc001twz.3. human.

Polymorphism databases

DMDMi68053233.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024460 mRNA. Translation: BAB15750.1. Different initiation.
BC030252 mRNA. Translation: AAH30252.1.
BC098404 mRNA. Translation: AAH98404.1.
AK023801 mRNA. Translation: BAB14685.1. Different initiation.
CCDSiCCDS44993.1.
RefSeqiNP_071936.2. NM_022491.2.
UniGeneiHs.416630.
Hs.602312.

3D structure databases

ProteinModelPortaliQ9H7L9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122174. 21 interactions.
IntActiQ9H7L9. 8 interactions.
STRINGi9606.ENSP00000380695.

PTM databases

PhosphoSiteiQ9H7L9.

Polymorphism databases

DMDMi68053233.

Proteomic databases

MaxQBiQ9H7L9.
PaxDbiQ9H7L9.
PRIDEiQ9H7L9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000543473; ENSP00000443988; ENSG00000111707.
GeneIDi64426.
KEGGihsa:64426.
UCSCiuc001twz.3. human.

Organism-specific databases

CTDi64426.
GeneCardsiGC12P118814.
HGNCiHGNC:29545. SUDS3.
HPAiHPA040402.
HPA041972.
MIMi608250. gene.
neXtProtiNX_Q9H7L9.
PharmGKBiPA143485626.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG311652.
GeneTreeiENSGT00530000063177.
HOGENOMiHOG000007054.
HOVERGENiHBG108465.
InParanoidiQ9H7L9.
OrthoDBiEOG7VQJFB.
PhylomeDBiQ9H7L9.
TreeFamiTF323740.

Enzyme and pathway databases

ReactomeiREACT_200856. NoRC negatively regulates rRNA expression.
REACT_228222. HDACs deacetylate histones.

Miscellaneous databases

ChiTaRSiSUDS3. human.
GeneWikiiSUDS3.
GenomeRNAii64426.
NextBioi66429.
PROiQ9H7L9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H7L9.
CleanExiHS_SUDS3.
ExpressionAtlasiQ9H7L9. baseline and differential.
GenevestigatoriQ9H7L9.

Family and domain databases

InterProiIPR013907. Sds3.
[Graphical view]
PfamiPF08598. Sds3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of long cDNA clones from human adult spleen."
    Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O.
    DNA Res. 7:357-366(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  2. "Identification and characterization of three new components of the mSin3A corepressor complex."
    Fleischer T.C., Yun U.J., Ayer D.E.
    Mol. Cell. Biol. 23:3456-3467(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION IN A MSIN3A COREPRESSOR COMPLEX WITH SIN3A; SAP130; SUDS3; ARID4B; HDAC1 AND HDAC2.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: LungImported and Placenta.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 130-328.
    Tissue: PlacentaImported and SpleenImported.
  5. "Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1."
    Wysocka J., Myers M.P., Laherty C.D., Eisenman R.N., Herr W.
    Genes Dev. 17:896-911(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HCFC1.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-49, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49; SER-53; SER-234 AND THR-244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
    Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
    Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-49, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49 AND SER-234, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "Lys-63-specific deubiquitination of SDS3 by USP17 regulates HDAC activity."
    Ramakrishna S., Suresh B., Lee E.J., Lee H.J., Ahn W.S., Baek K.H.
    J. Biol. Chem. 286:10505-10514(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH USP17L2, SUBCELLULAR LOCATION, UBIQUITINATION, DEUBIQUITINATION BY USP17L2.
  12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-45; SER-228 AND SER-234, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSDS3_HUMAN
AccessioniPrimary (citable) accession number: Q9H7L9
Secondary accession number(s): Q4KMQ5, Q8N6H0, Q9H8D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: February 4, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.