Q9H7L9 (SDS3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sin3 histone deacetylase corepressor complex component SDS3

Alternative name(s):
45 kDa Sin3-associated polypeptide
Suppressor of defective silencing 3 protein homolog

Gene names

Name:	SUDS3
Synonyms:	SAP45, SDS3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways through regulation of apoptosis. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Ref.2 Ref.11 UniProtKB Q8BR65
Subunit structure	Homodimer. Component of the SIN3 histone deacetylase (HDAC) corepressor complex. Interacts with SIN3A. Interaction with SIN3B enhances the interaction between SIN3B and HDAC1 to form a complex By similarity. Interacts with HCFC1. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with USP17L2; the interaction is direct. Ref.2 Ref.5 Ref.11 UniProtKB Q8BR65
Subcellular location	Nucleus Ref.11.
Tissue specificity	Expressed in various cancer cell ines. Ref.2
Domain	The C-terminus is involved in transcriptional repression by HDAC-independent mechanisms.
Post-translational modification	Polyubiquitinated. 'Lys-63'-polyubiquitinated SUDS3 positively regulates histone deacetylation. Regulated through deubiquitination by USP17L2/USP17 that cleaves 'Lys-63'-linked ubiquitin chains.
Sequence similarities	Belongs to the SDS3 family.
Sequence caution	The sequence BAB14685.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15750.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Apoptosis Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Chromatin regulator Repressor
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW histone deacetylation Inferred from direct assay Ref.11. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 22783022. Source: BHF-UCL positive regulation of apoptotic process Inferred from mutant phenotype Ref.11. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Sin3 complex Inferred from sequence or structural similarity PubMed 11909966. Source: UniProtKB
Molecular_function	histone deacetylase binding Inferred from sequence or structural similarity PubMed 11909966. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRMS1	Q9HCU9	2	EBI-540496,EBI-714781
HCFC1	P51610	2	EBI-540496,EBI-396176

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.12
	Chain	2 – 328	327	Sin3 histone deacetylase corepressor complex component SDS3		PRO_0000097652
Regions
	Region	2 – 170	169	Mediates interaction with USP17L2
	Region	188 – 226	39	Sin3 interaction domain (SID) By similarity UniProtKB Q8BR65
	Coiled coil	66 – 171	106	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.12
	Modified residue	32	1	Phosphoserine Ref.12
	Modified residue	45	1	Phosphoserine Ref.6 Ref.7 Ref.8 Ref.10 Ref.12
	Modified residue	49	1	Phosphothreonine Ref.6 Ref.7 Ref.8 Ref.10
	Modified residue	53	1	Phosphoserine Ref.7
	Modified residue	228	1	Phosphoserine Ref.12
	Modified residue	234	1	Phosphoserine Ref.7 Ref.9 Ref.10 Ref.12
	Modified residue	244	1	Phosphothreonine Ref.7
Experimental info
	Sequence conflict	164 – 171	8	EKLTMELT → HASAHASA in AAH30252. Ref.2
	Sequence conflict	286	1	S → R in BAB14685. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9H7L9 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 10A7393B607117EB	FASTA	328	38,136
	10 20 30 40 50 60 MSAAGLLAPA PAQAGAPPAP EYYPEEDEEL ESAEDDERSC RGRESDEDTE DASETDLAKH 70 80 90 100 110 120 DEEDYVEMKE QMYQDKLASL KRQLQQLQEG TLQEYQKRMK KLDQQYKERI RNAELFLQLE 130 140 150 160 170 180 TEQVERNYIK EKKAAVKEFE DKKVELKENL IAELEEKKKM IENEKLTMEL TGDSMEVKPI 190 200 210 220 230 240 MTRKLRRRPN DPVPIPDKRR KPAPAQLNYL LTDEQIMEDL RTLNKLKSPK RPASPSSPEH 250 260 270 280 290 300 LPATPAESPA QRFEARIEDG KLYYDKRWYH KSQAIYLESK DNQKLSCVIS SVGANEIWVR 310 320 KTSDSTKMRI YLGQLQRGLF VIRRRSAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of long cDNA clones from human adult spleen." Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O. DNA Res. 7:357-366(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[2]	"Identification and characterization of three new components of the mSin3A corepressor complex." Fleischer T.C., Yun U.J., Ayer D.E. Mol. Cell. Biol. 23:3456-3467(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION IN A MSIN3A COREPRESSOR COMPLEX WITH SIN3A; SAP130; SUDS3; ARID4B; HDAC1 AND HDAC2.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Placenta.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 130-328. Tissue: Placenta and Spleen.
[5]	"Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1." Wysocka J., Myers M.P., Laherty C.D., Eisenman R.N., Herr W. Genes Dev. 17:896-911(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HCFC1.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-49, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49; SER-53; SER-234 AND THR-244, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-49, MASS SPECTROMETRY. Tissue: Liver.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49 AND SER-234, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Lys-63-specific deubiquitination of SDS3 by USP17 regulates HDAC activity." Ramakrishna S., Suresh B., Lee E.J., Lee H.J., Ahn W.S., Baek K.H. J. Biol. Chem. 286:10505-10514(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH USP17L2, SUBCELLULAR LOCATION, UBIQUITINATION, DEUBIQUITINATION BY USP17L2.
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-45; SER-228 AND SER-234, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK024460 mRNA. Translation: BAB15750.1. Different initiation. BC030252 mRNA. Translation: AAH30252.1. BC098404 mRNA. Translation: AAH98404.1. AK023801 mRNA. Translation: BAB14685.1. Different initiation.
IPI	IPI00607645.
RefSeq	NP_071936.2. NM_022491.2.
UniGene	Hs.416630. Hs.602312.
3D structure databases
ProteinModelPortal	Q9H7L9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H7L9. 4 interactions.
STRING	9606.ENSP00000380695.
PTM databases
PhosphoSite	Q9H7L9.
Polymorphism databases
DMDM	68053233.
Proteomic databases
PaxDb	Q9H7L9.
PRIDE	Q9H7L9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000543473; ENSP00000443988; ENSG00000111707.
GeneID	64426.
KEGG	hsa:64426.
UCSC	uc001twz.3. human.
Organism-specific databases
CTD	64426.
GeneCards	GC12P118814.
HGNC	HGNC:29545. SUDS3.
HPA	HPA040402. HPA041972.
MIM	608250. gene.
neXtProt	NX_Q9H7L9.
PharmGKB	PA143485626.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG311652.
HOGENOM	HOG000007054.
HOVERGEN	HBG108465.
InParanoid	Q9H7L9.
OrthoDB	EOG4N8R5F.
Gene expression databases
ArrayExpress	Q9H7L9.
Bgee	Q9H7L9.
CleanEx	HS_SUDS3.
Genevestigator	Q9H7L9.
GermOnline	ENSG00000111707. Homo sapiens.
Family and domain databases
InterPro	IPR013907. Sds3. [Graphical view]
Pfam	PF08598. Sds3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SUDS3. human.
GenomeRNAi	64426.
NextBio	66429.
SOURCE	Search...

Entry information

Entry name

SDS3_HUMAN

Accession

Primary (citable) accession number: Q9H7L9
Secondary accession number(s): Q4KMQ5, Q8N6H0, Q9H8D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.