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Protein

Exonuclease V

Gene

EXO5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication
  • Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92Iron-sulfur (4Fe-4S)1
Metal bindingi343Iron-sulfur (4Fe-4S)1
Metal bindingi346Iron-sulfur (4Fe-4S)1
Metal bindingi352Iron-sulfur (4Fe-4S)1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB
  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein homodimerization activity Source: UniProtKB
  • single-stranded DNA 3'-5' exodeoxyribonuclease activity Source: UniProtKB
  • single-stranded DNA 5'-3' exodeoxyribonuclease activity Source: UniProtKB

GO - Biological processi

  • interstrand cross-link repair Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Exonuclease V (EC:3.1.-.-)
Short name:
Exo V
Short name:
hExo5
Alternative name(s):
Defects in morphology protein 1 homolog
Gene namesi
Name:EXO5
Synonyms:C1orf176, DEM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:26115. EXO5.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasmcytosol 1 Publication

  • Note: Localizes to repair foci in response to DNA damage.

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi196E → A: Nearly abolishes exonuclease activity. 1 Publication1
Mutagenesisi343C → A: Abolishes iron-sulfur-binding and affects exonuclease activity; when associated with A-346. 1 Publication1
Mutagenesisi346C → A: Abolishes iron-sulfur-binding and affects exonuclease activity; when associated with A-343. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000164002.
PharmGKBiPA164718736.

Polymorphism and mutation databases

BioMutaiEXO5.
DMDMi74752706.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003073201 – 373Exonuclease VAdd BLAST373

Proteomic databases

MaxQBiQ9H790.
PaxDbiQ9H790.
PeptideAtlasiQ9H790.
PRIDEiQ9H790.

PTM databases

iPTMnetiQ9H790.
PhosphoSitePlusiQ9H790.

Expressioni

Gene expression databases

BgeeiENSG00000164002.
CleanExiHS_DEM1.
ExpressionAtlasiQ9H790. baseline and differential.
GenevisibleiQ9H790. HS.

Organism-specific databases

HPAiHPA028429.

Interactioni

Subunit structurei

Monomer; monomeric form has weak exonuclease activity. Homodimer; homodimeric form is unsure but has much higher exonuclease activity, suggesting that it could homodimerize upon DNA-binding. Interacts with the replication protein A (RPA) complex.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi122299. 2 interactors.
STRINGi9606.ENSP00000296380.

Structurei

3D structure databases

ProteinModelPortaliQ9H790.
SMRiQ9H790.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EXO5 family.Curated

Phylogenomic databases

eggNOGiKOG4760. Eukaryota.
ENOG4111IID. LUCA.
GeneTreeiENSGT00390000015205.
HOVERGENiHBG095898.
InParanoidiQ9H790.
KOiK17815.
OMAiEEMALID.
OrthoDBiEOG091G0M7G.
PhylomeDBiQ9H790.
TreeFamiTF332529.

Family and domain databases

InterProiIPR019190. EXOV.
[Graphical view]
PfamiPF09810. Exo5. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETREEETV SAEASGFSDL SDSEFLEFLD LEDAQESKAL VNMPGPSSES
60 70 80 90 100
LGKDDKPISL QNWKRGLDIL SPMERFHLKY LYVTDLATQN WCELQTAYGK
110 120 130 140 150
ELPGFLAPEK AAVLDTGASI HLARELELHD LVTVPVTTKE DAWAIKFLNI
160 170 180 190 200
LLLIPTLQSE GHIREFPVFG EGEGVLLVGV IDELHYTAKG ELELAELKTR
210 220 230 240 250
RRPMLPLEAQ KKKDCFQVSL YKYIFDAMVQ GKVTPASLIH HTKLCLEKPL
260 270 280 290 300
GPSVLRHAQQ GGFSVKSLGD LMELVFLSLT LSDLPVIDIL KIEYIHQETA
310 320 330 340 350
TVLGTEIVAF KEKEVRAKVQ HYMAYWMGHR EPQGVDVEEA WKCRTCTYAD
360 370
ICEWRKGSGV LSSTLAPQVK KAK
Length:373
Mass (Da):41,816
Last modified:March 1, 2001 - v1
Checksum:iEAFB31099EA40FAD
GO

Sequence cautioni

The sequence CAI16298 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI16299 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI16300 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI16301 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI16302 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035407115D → N Polymorphism; does not affect exonuclease activity. 1 PublicationCorresponds to variant rs1134586dbSNPEnsembl.1
Natural variantiVAR_035408172G → V Polymorphism; does not affect exonuclease activity. 2 PublicationsCorresponds to variant rs11208299dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024797 mRNA. Translation: BAB15008.1.
AL603839 Genomic DNA. Translation: CAI16298.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16299.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16300.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16301.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16302.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16303.1.
CH471059 Genomic DNA. Translation: EAX07210.1.
CH471059 Genomic DNA. Translation: EAX07211.1.
CH471059 Genomic DNA. Translation: EAX07212.1.
CH471059 Genomic DNA. Translation: EAX07213.1.
CH471059 Genomic DNA. Translation: EAX07214.1.
BC021969 mRNA. Translation: AAH21969.1.
CCDSiCCDS453.1.
RefSeqiNP_073611.1. NM_022774.1.
XP_005271182.1. XM_005271125.3.
XP_005271183.1. XM_005271126.3.
XP_005271185.1. XM_005271128.3.
XP_011540272.1. XM_011541970.2.
XP_016857588.1. XM_017002099.1.
XP_016857589.1. XM_017002100.1.
XP_016857590.1. XM_017002101.1.
XP_016857591.1. XM_017002102.1.
XP_016857592.1. XM_017002103.1.
XP_016857593.1. XM_017002104.1.
UniGeneiHs.59584.

Genome annotation databases

EnsembliENST00000296380; ENSP00000296380; ENSG00000164002.
ENST00000358527; ENSP00000351328; ENSG00000164002.
ENST00000372703; ENSP00000361788; ENSG00000164002.
GeneIDi64789.
KEGGihsa:64789.
UCSCiuc001cfp.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024797 mRNA. Translation: BAB15008.1.
AL603839 Genomic DNA. Translation: CAI16298.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16299.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16300.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16301.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16302.1. Sequence problems.
AL603839 Genomic DNA. Translation: CAI16303.1.
CH471059 Genomic DNA. Translation: EAX07210.1.
CH471059 Genomic DNA. Translation: EAX07211.1.
CH471059 Genomic DNA. Translation: EAX07212.1.
CH471059 Genomic DNA. Translation: EAX07213.1.
CH471059 Genomic DNA. Translation: EAX07214.1.
BC021969 mRNA. Translation: AAH21969.1.
CCDSiCCDS453.1.
RefSeqiNP_073611.1. NM_022774.1.
XP_005271182.1. XM_005271125.3.
XP_005271183.1. XM_005271126.3.
XP_005271185.1. XM_005271128.3.
XP_011540272.1. XM_011541970.2.
XP_016857588.1. XM_017002099.1.
XP_016857589.1. XM_017002100.1.
XP_016857590.1. XM_017002101.1.
XP_016857591.1. XM_017002102.1.
XP_016857592.1. XM_017002103.1.
XP_016857593.1. XM_017002104.1.
UniGeneiHs.59584.

3D structure databases

ProteinModelPortaliQ9H790.
SMRiQ9H790.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122299. 2 interactors.
STRINGi9606.ENSP00000296380.

PTM databases

iPTMnetiQ9H790.
PhosphoSitePlusiQ9H790.

Polymorphism and mutation databases

BioMutaiEXO5.
DMDMi74752706.

Proteomic databases

MaxQBiQ9H790.
PaxDbiQ9H790.
PeptideAtlasiQ9H790.
PRIDEiQ9H790.

Protocols and materials databases

DNASUi64789.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296380; ENSP00000296380; ENSG00000164002.
ENST00000358527; ENSP00000351328; ENSG00000164002.
ENST00000372703; ENSP00000361788; ENSG00000164002.
GeneIDi64789.
KEGGihsa:64789.
UCSCiuc001cfp.4. human.

Organism-specific databases

CTDi64789.
GeneCardsiEXO5.
HGNCiHGNC:26115. EXO5.
HPAiHPA028429.
neXtProtiNX_Q9H790.
OpenTargetsiENSG00000164002.
PharmGKBiPA164718736.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4760. Eukaryota.
ENOG4111IID. LUCA.
GeneTreeiENSGT00390000015205.
HOVERGENiHBG095898.
InParanoidiQ9H790.
KOiK17815.
OMAiEEMALID.
OrthoDBiEOG091G0M7G.
PhylomeDBiQ9H790.
TreeFamiTF332529.

Miscellaneous databases

GenomeRNAii64789.
PROiQ9H790.

Gene expression databases

BgeeiENSG00000164002.
CleanExiHS_DEM1.
ExpressionAtlasiQ9H790. baseline and differential.
GenevisibleiQ9H790. HS.

Family and domain databases

InterProiIPR019190. EXOV.
[Graphical view]
PfamiPF09810. Exo5. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEXO5_HUMAN
AccessioniPrimary (citable) accession number: Q9H790
Secondary accession number(s): D3DPV4
, Q5SWM7, Q5SWM8, Q5SWM9, Q5SWN0, Q5SWN1, Q8WTW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.