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Q9H790

- EXO5_HUMAN

UniProt

Q9H790 - EXO5_HUMAN

Protein

Exonuclease V

Gene

EXO5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity.1 Publication

    Cofactori

    Binds 1 4Fe-4S cluster.1 Publication
    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi92 – 921Iron-sulfur (4Fe-4S)
    Metal bindingi343 – 3431Iron-sulfur (4Fe-4S)
    Metal bindingi346 – 3461Iron-sulfur (4Fe-4S)
    Metal bindingi352 – 3521Iron-sulfur (4Fe-4S)

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB
    2. DNA binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. protein homodimerization activity Source: UniProtKB
    5. single-stranded DNA 3'-5' exodeoxyribonuclease activity Source: UniProtKB
    6. single-stranded DNA 5'-3' exodeoxyribonuclease activity Source: UniProtKB

    GO - Biological processi

    1. DNA catabolic process, exonucleolytic Source: GOC
    2. interstrand cross-link repair Source: UniProtKB

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    4Fe-4S, DNA-binding, Iron, Iron-sulfur, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exonuclease V (EC:3.1.-.-)
    Short name:
    Exo V
    Short name:
    hExo5
    Alternative name(s):
    Defects in morphology protein 1 homolog
    Gene namesi
    Name:EXO5
    Synonyms:C1orf176, DEM1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:26115. EXO5.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasmcytosol 1 Publication
    Note: Localizes to repair foci in response to DNA damage.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi196 – 1961E → A: Nearly abolishes exonuclease activity. 1 Publication
    Mutagenesisi343 – 3431C → A: Abolishes iron-sulfur-binding and affects exonuclease activity; when associated with A-346. 1 Publication
    Mutagenesisi346 – 3461C → A: Abolishes iron-sulfur-binding and affects exonuclease activity; when associated with A-343. 1 Publication

    Organism-specific databases

    PharmGKBiPA164718736.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 373373Exonuclease VPRO_0000307320Add
    BLAST

    Proteomic databases

    MaxQBiQ9H790.
    PaxDbiQ9H790.
    PRIDEiQ9H790.

    PTM databases

    PhosphoSiteiQ9H790.

    Expressioni

    Gene expression databases

    BgeeiQ9H790.
    CleanExiHS_DEM1.
    GenevestigatoriQ9H790.

    Organism-specific databases

    HPAiHPA028429.

    Interactioni

    Subunit structurei

    Monomer; monomeric form has weak exonuclease activity. Homodimer; homodimeric form is unsure but has much higher exonuclease activity, suggesting that it could homodimerize upon DNA-binding. Interacts with the replication protein A (RPA) complex.1 Publication

    Protein-protein interaction databases

    BioGridi122299. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H790.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the EXO5 family.Curated

    Phylogenomic databases

    eggNOGiNOG331822.
    HOVERGENiHBG095898.
    InParanoidiQ9H790.
    KOiK17815.
    OMAiIFDAMVQ.
    OrthoDBiEOG761BTW.
    PhylomeDBiQ9H790.
    TreeFamiTF332529.

    Family and domain databases

    InterProiIPR019190. EXOV.
    [Graphical view]
    PfamiPF09810. Exo5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9H790-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAETREEETV SAEASGFSDL SDSEFLEFLD LEDAQESKAL VNMPGPSSES    50
    LGKDDKPISL QNWKRGLDIL SPMERFHLKY LYVTDLATQN WCELQTAYGK 100
    ELPGFLAPEK AAVLDTGASI HLARELELHD LVTVPVTTKE DAWAIKFLNI 150
    LLLIPTLQSE GHIREFPVFG EGEGVLLVGV IDELHYTAKG ELELAELKTR 200
    RRPMLPLEAQ KKKDCFQVSL YKYIFDAMVQ GKVTPASLIH HTKLCLEKPL 250
    GPSVLRHAQQ GGFSVKSLGD LMELVFLSLT LSDLPVIDIL KIEYIHQETA 300
    TVLGTEIVAF KEKEVRAKVQ HYMAYWMGHR EPQGVDVEEA WKCRTCTYAD 350
    ICEWRKGSGV LSSTLAPQVK KAK 373
    Length:373
    Mass (Da):41,816
    Last modified:March 1, 2001 - v1
    Checksum:iEAFB31099EA40FAD
    GO

    Sequence cautioni

    The sequence CAI16298.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAI16299.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAI16300.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAI16301.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAI16302.1 differs from that shown. Reason: Erroneous gene model prediction.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti115 – 1151D → N Polymorphism that does not affect exonuclease activity.
    Corresponds to variant rs1134586 [ dbSNP | Ensembl ].
    VAR_035407
    Natural varianti172 – 1721G → V Polymorphism that does not affect exonuclease activity. 1 Publication
    Corresponds to variant rs11208299 [ dbSNP | Ensembl ].
    VAR_035408

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK024797 mRNA. Translation: BAB15008.1.
    AL603839 Genomic DNA. Translation: CAI16298.1. Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16299.1. Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16300.1. Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16301.1. Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16302.1. Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16303.1.
    CH471059 Genomic DNA. Translation: EAX07210.1.
    CH471059 Genomic DNA. Translation: EAX07211.1.
    CH471059 Genomic DNA. Translation: EAX07212.1.
    CH471059 Genomic DNA. Translation: EAX07213.1.
    CH471059 Genomic DNA. Translation: EAX07214.1.
    BC021969 mRNA. Translation: AAH21969.1.
    CCDSiCCDS453.1.
    RefSeqiNP_073611.1. NM_022774.1.
    XP_005271182.1. XM_005271125.1.
    XP_005271183.1. XM_005271126.1.
    XP_005271185.1. XM_005271128.1.
    UniGeneiHs.59584.

    Genome annotation databases

    EnsembliENST00000296380; ENSP00000296380; ENSG00000164002.
    ENST00000358527; ENSP00000351328; ENSG00000164002.
    ENST00000372703; ENSP00000361788; ENSG00000164002.
    GeneIDi64789.
    KEGGihsa:64789.
    UCSCiuc001cfp.3. human.

    Polymorphism databases

    DMDMi74752706.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK024797 mRNA. Translation: BAB15008.1 .
    AL603839 Genomic DNA. Translation: CAI16298.1 . Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16299.1 . Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16300.1 . Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16301.1 . Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16302.1 . Sequence problems.
    AL603839 Genomic DNA. Translation: CAI16303.1 .
    CH471059 Genomic DNA. Translation: EAX07210.1 .
    CH471059 Genomic DNA. Translation: EAX07211.1 .
    CH471059 Genomic DNA. Translation: EAX07212.1 .
    CH471059 Genomic DNA. Translation: EAX07213.1 .
    CH471059 Genomic DNA. Translation: EAX07214.1 .
    BC021969 mRNA. Translation: AAH21969.1 .
    CCDSi CCDS453.1.
    RefSeqi NP_073611.1. NM_022774.1.
    XP_005271182.1. XM_005271125.1.
    XP_005271183.1. XM_005271126.1.
    XP_005271185.1. XM_005271128.1.
    UniGenei Hs.59584.

    3D structure databases

    ProteinModelPortali Q9H790.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122299. 3 interactions.

    PTM databases

    PhosphoSitei Q9H790.

    Polymorphism databases

    DMDMi 74752706.

    Proteomic databases

    MaxQBi Q9H790.
    PaxDbi Q9H790.
    PRIDEi Q9H790.

    Protocols and materials databases

    DNASUi 64789.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000296380 ; ENSP00000296380 ; ENSG00000164002 .
    ENST00000358527 ; ENSP00000351328 ; ENSG00000164002 .
    ENST00000372703 ; ENSP00000361788 ; ENSG00000164002 .
    GeneIDi 64789.
    KEGGi hsa:64789.
    UCSCi uc001cfp.3. human.

    Organism-specific databases

    CTDi 64789.
    GeneCardsi GC01P040975.
    HGNCi HGNC:26115. EXO5.
    HPAi HPA028429.
    neXtProti NX_Q9H790.
    PharmGKBi PA164718736.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG331822.
    HOVERGENi HBG095898.
    InParanoidi Q9H790.
    KOi K17815.
    OMAi IFDAMVQ.
    OrthoDBi EOG761BTW.
    PhylomeDBi Q9H790.
    TreeFami TF332529.

    Miscellaneous databases

    GenomeRNAii 64789.
    NextBioi 66850.
    PROi Q9H790.

    Gene expression databases

    Bgeei Q9H790.
    CleanExi HS_DEM1.
    Genevestigatori Q9H790.

    Family and domain databases

    InterProi IPR019190. EXOV.
    [Graphical view ]
    Pfami PF09810. Exo5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Coronary artery.
    2. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-172.
      Tissue: Ovary.
    5. "Human exonuclease 5 is a novel sliding exonuclease required for genome stability."
      Sparks J.L., Kumar R., Singh M., Wold M.S., Pandita T.K., Burgers P.M.
      J. Biol. Chem. 287:42773-42783(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, COFACTOR, INTERACTION WITH THE REPLICATION PROTEIN A (RPA) COMPLEX, MUTAGENESIS OF GLU-196; CYS-343 AND CYS-346, CHARACTERIZATION OF VARIANTS ASN-115 AND VAL-172.

    Entry informationi

    Entry nameiEXO5_HUMAN
    AccessioniPrimary (citable) accession number: Q9H790
    Secondary accession number(s): D3DPV4
    , Q5SWM7, Q5SWM8, Q5SWM9, Q5SWN0, Q5SWN1, Q8WTW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3