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Protein

dCTP pyrophosphatase 1

Gene

DCTPP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. Has a strong preference for dCTP and its analogs including 5-iodo-dCTP and 5-methyl-dCTP for which it may even have a higher efficiency. May protect DNA or RNA against the incorporation of these genotoxic nucleotide analogs through their catabolism.1 Publication

Catalytic activityi

dCTP + H2O = dCMP + diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Probably binds two or three Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by the reaction end product PPi. Inhibited by dCDP. Inhibited by triptolide.1 Publication

Kineticsi

  1. KM=47.6 µM for dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  2. KM=65 µM for dTTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  3. KM=79.3 µM for dATP (at pH 8.0 and 25 degrees Celsius)1 Publication
  4. KM=75.9 µM for dUTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  5. KM=16.8 µM for 5I-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  6. KM=36.5 µM for 5Br-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  7. KM=33.3 µM for 5me-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  8. KM=62.9 µM for 5hme-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  9. KM=9.2 µM for 5fo-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  10. KM=142.9 µM for CTP (at pH 8.0 and 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei38SubstrateBy similarity1
    Metal bindingi63MagnesiumBy similarity1
    Metal bindingi66MagnesiumBy similarity1
    Binding sitei73SubstrateBy similarity1
    Metal bindingi95MagnesiumBy similarity1
    Metal bindingi98MagnesiumBy similarity1
    Binding sitei102SubstrateBy similarity1

    GO - Molecular functioni

    • dCTP diphosphatase activity Source: UniProtKB
    • identical protein binding Source: IntAct
    • magnesium ion binding Source: UniProtKB
    • nucleoside-triphosphate diphosphatase activity Source: UniProtKB
    • pyrimidine deoxyribonucleotide binding Source: Ensembl

    GO - Biological processi

    • dCTP catabolic process Source: UniProtKB
    • DNA protection Source: UniProtKB
    • nucleoside triphosphate catabolic process Source: UniProtKB
    • protein homotetramerization Source: Ensembl

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiR-HSA-499943. Interconversion of nucleotide di- and triphosphates.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    dCTP pyrophosphatase 1Curated (EC:3.6.1.121 Publication)
    Alternative name(s):
    Deoxycytidine-triphosphatase 1
    Short name:
    dCTPase 1
    RS21C6Imported
    XTP3-transactivated gene A proteinImported
    Gene namesi
    Name:DCTPP1Imported
    Synonyms:XTP3TPAImported
    ORF Names:CDA03Imported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 16

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000179958.8.
    HGNCiHGNC:28777. DCTPP1.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi63E → Q: Loss of dCTP diphosphatase activity. 1 Publication1

    Organism-specific databases

    OpenTargetsiENSG00000179958.
    PharmGKBiPA164718733.

    Chemistry databases

    ChEMBLiCHEMBL3769292.

    Polymorphism and mutation databases

    BioMutaiDCTPP1.
    DMDMi74733624.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources
    ChainiPRO_00002917692 – 170dCTP pyrophosphatase 1Add BLAST169

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserineCombined sources1
    Modified residuei2PhosphoserineCombined sources1
    Modified residuei12PhosphothreonineCombined sources1
    Modified residuei85PhosphoserineCombined sources1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    EPDiQ9H773.
    MaxQBiQ9H773.
    PaxDbiQ9H773.
    PeptideAtlasiQ9H773.
    PRIDEiQ9H773.
    TopDownProteomicsiQ9H773.

    PTM databases

    iPTMnetiQ9H773.
    PhosphoSitePlusiQ9H773.
    SwissPalmiQ9H773.

    Expressioni

    Developmental stagei

    Expression increases during mitosis (at protein level).1 Publication

    Inductioni

    Up-regulated by an increase in cellular dCTP pool.1 Publication

    Gene expression databases

    BgeeiENSG00000179958.
    ExpressionAtlasiQ9H773. baseline and differential.
    GenevisibleiQ9H773. HS.

    Organism-specific databases

    HPAiHPA002832.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Binary interactionsi

    Show more details

    GO - Molecular functioni

    • identical protein binding Source: IntAct

    Protein-protein interaction databases

    BioGridi122527. 32 interactors.
    IntActiQ9H773. 12 interactors.
    STRINGi9606.ENSP00000322524.

    Chemistry databases

    BindingDBiQ9H773.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H773.
    SMRiQ9H773.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Repeati29 – 75EARAdd BLAST47

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni47 – 51Substrate bindingBy similarity5

    Phylogenomic databases

    eggNOGiENOG410IXWQ. Eukaryota.
    ENOG4111PYX. LUCA.
    GeneTreeiENSGT00390000017709.
    HOGENOMiHOG000056700.
    InParanoidiQ9H773.
    KOiK16904.
    OMAiELFQWKT.
    OrthoDBiEOG091G0RYZ.
    PhylomeDBiQ9H773.
    TreeFamiTF300237.

    Family and domain databases

    CDDicd11537. NTP-PPase_RS21-C6_like. 1 hit.
    InterProiView protein in InterPro
    IPR025984. DCTPP.
    IPR009039. EAR.
    PfamiView protein in Pfam
    PF12643. MazG-like. 1 hit.
    PIRSFiPIRSF029826. UCP029826_pph. 1 hit.
    PROSITEiView protein in PROSITE
    PS50912. EAR. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9H773-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSVAGGEIRG DTGGEDTAAP GRFSFSPEPT LEDIRRLHAE FAAERDWEQF
    60 70 80 90 100
    HQPRNLLLAL VGEVGELAEL FQWKTDGEPG PQGWSPRERA ALQEELSDVL
    110 120 130 140 150
    IYLVALAARC RVDLPLAVLS KMDINRRRYP AHLARSSSRK YTELPHGAIS
    160 170
    EDQAVGPADI PCDSTGQTST
    Length:170
    Mass (Da):18,681
    Last modified:March 1, 2001 - v1
    Checksum:i23BC932D5461320B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY453409 mRNA. Translation: AAR26724.1.
    AF212242 mRNA. Translation: AAK14927.1.
    AF210430 mRNA. Translation: AAK48422.1.
    CR457335 mRNA. Translation: CAG33616.1.
    AK024843 mRNA. Translation: BAB15025.1.
    BC001344 mRNA. Translation: AAH01344.1.
    CCDSiCCDS10680.1.
    RefSeqiNP_077001.1. NM_024096.1.
    UniGeneiHs.632191.

    Genome annotation databases

    EnsembliENST00000319285; ENSP00000322524; ENSG00000179958.
    GeneIDi79077.
    KEGGihsa:79077.
    UCSCiuc002dyf.4. human.

    Similar proteinsi

    Entry informationi

    Entry nameiDCTP1_HUMAN
    AccessioniPrimary (citable) accession number: Q9H773
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: March 1, 2001
    Last modified: October 25, 2017
    This is version 132 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot