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Q9H773 (DCTP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
dCTP pyrophosphatase 1

EC=3.6.1.12
Alternative name(s):
Deoxycytidine-triphosphatase 1
Short name=dCTPase 1
RS21C6
XTP3-transactivated gene A protein
Gene names
Name:DCTPP1
Synonyms:XTP3TPA
ORF Names:CDA03
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length170 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. Has a strong preference for modified dCTP. Activity is highest with 5-iodo-dCTP, followed by 5-bromo-dCTP, unmodified dCTP, 5-methyl-dCTP and 5-chloro-dCTP. Hydrolyzes 2-chloro-dATP and 2-hydroxy-dATP with lower efficiency, and has even lower activity with unmodified dATP, dTTP and dUTP (in vitro). Does not hydrolyze ATP, UTP, ITP, GTP, dADP, dCDP or dGTP. May protect DNA or RNA against the incorporation of non-canonical nucleotide triphosphates. May protect cells against inappropriate methylation of CpG islands by DNA methyltransferases By similarity.

Catalytic activity

dCTP + H2O = dCMP + diphosphate.

Cofactor

Magnesium. Probably binds two or three magnesium ions per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Contains 1 EAR repeat.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 170169dCTP pyrophosphatase 1
PRO_0000291769

Regions

Repeat29 – 7547EAR
Region47 – 515Substrate binding By similarity

Sites

Metal binding631Magnesium By similarity
Metal binding661Magnesium By similarity
Metal binding951Magnesium By similarity
Metal binding981Magnesium By similarity
Binding site381Substrate By similarity
Binding site731Substrate By similarity
Binding site1021Substrate By similarity

Amino acid modifications

Modified residue21N-acetylserine Ref.9 Ref.11
Modified residue21Phosphoserine Ref.7
Modified residue121Phosphothreonine Ref.7
Modified residue851Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q9H773 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 23BC932D5461320B

FASTA17018,681
        10         20         30         40         50         60 
MSVAGGEIRG DTGGEDTAAP GRFSFSPEPT LEDIRRLHAE FAAERDWEQF HQPRNLLLAL 

        70         80         90        100        110        120 
VGEVGELAEL FQWKTDGEPG PQGWSPRERA ALQEELSDVL IYLVALAARC RVDLPLAVLS 

       130        140        150        160        170 
KMDINRRRYP AHLARSSSRK YTELPHGAIS EDQAVGPADI PCDSTGQTST 

« Hide

References

« Hide 'large scale' references
[1]"Screening and cloning of the target genes transactivated by XTP3 using a suppression subtractive hybridization technique."
Wang C., Cheng J., Lang Z., Wu Y., Yang Y., Zhang L., Ji D.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A novel gene expressed in human pheochromocytoma."
Li Y., Huang Q., Peng Y., Song H., Yu Y., Xu S., Ren S., Chen Z., Han Z.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pheochromocytoma.
[3]Zhang J., Chen W.F., Wang H.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cervix.
[7]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; THR-12 AND SER-85, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY453409 mRNA. Translation: AAR26724.1.
AF212242 mRNA. Translation: AAK14927.1.
AF210430 mRNA. Translation: AAK48422.1.
CR457335 mRNA. Translation: CAG33616.1.
AK024843 mRNA. Translation: BAB15025.1.
BC001344 mRNA. Translation: AAH01344.1.
CCDSCCDS10680.1.
RefSeqNP_077001.1. NM_024096.1.
UniGeneHs.632191.

3D structure databases

ProteinModelPortalQ9H773.
SMRQ9H773. Positions 23-123.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122527. 21 interactions.
IntActQ9H773. 4 interactions.
STRING9606.ENSP00000322524.

Chemistry

BindingDBQ9H773.

PTM databases

PhosphoSiteQ9H773.

Polymorphism databases

DMDM74733624.

Proteomic databases

MaxQBQ9H773.
PaxDbQ9H773.
PeptideAtlasQ9H773.
PRIDEQ9H773.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000319285; ENSP00000322524; ENSG00000179958.
GeneID79077.
KEGGhsa:79077.
UCSCuc002dyf.3. human.

Organism-specific databases

CTD79077.
GeneCardsGC16M030435.
HGNCHGNC:28777. DCTPP1.
HPAHPA002832.
neXtProtNX_Q9H773.
PharmGKBPA164718733.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG131091.
HOGENOMHOG000056700.
InParanoidQ9H773.
KOK16904.
OMAGPQAWPP.
OrthoDBEOG70S774.
PhylomeDBQ9H773.
TreeFamTF300237.

Gene expression databases

ArrayExpressQ9H773.
BgeeQ9H773.
GenevestigatorQ9H773.

Family and domain databases

InterProIPR009039. EAR.
IPR004518. MazG_cat.
[Graphical view]
PfamPF03819. MazG. 1 hit.
[Graphical view]
PROSITEPS50912. EAR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiXTP3-transactivated_gene_A_protein.
GenomeRNAi79077.
NextBio67876.
PROQ9H773.

Entry information

Entry nameDCTP1_HUMAN
AccessionPrimary (citable) accession number: Q9H773
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM