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Protein

dCTP pyrophosphatase 1

Gene

DCTPP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. Has a strong preference for dCTP and its analogs including 5-iodo-dCTP and 5-methyl-dCTP for which it may even have a higher efficiency. May protect DNA or RNA against the incorporation of these genotoxic nucleotide analogs through their catabolism.1 Publication

Catalytic activityi

dCTP + H2O = dCMP + diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Probably binds two or three Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by the reaction end product PPi. Inhibited by dCDP. Inhibited by triptolide.1 Publication

Kineticsi

  1. KM=47.6 µM for dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  2. KM=65 µM for dTTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  3. KM=79.3 µM for dATP (at pH 8.0 and 25 degrees Celsius)1 Publication
  4. KM=75.9 µM for dUTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  5. KM=16.8 µM for 5I-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  6. KM=36.5 µM for 5Br-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  7. KM=33.3 µM for 5me-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  8. KM=62.9 µM for 5hme-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  9. KM=9.2 µM for 5fo-dCTP (at pH 8.0 and 25 degrees Celsius)1 Publication
  10. KM=142.9 µM for CTP (at pH 8.0 and 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei38SubstrateBy similarity1
    Metal bindingi63MagnesiumBy similarity1
    Metal bindingi66MagnesiumBy similarity1
    Binding sitei73SubstrateBy similarity1
    Metal bindingi95MagnesiumBy similarity1
    Metal bindingi98MagnesiumBy similarity1
    Binding sitei102SubstrateBy similarity1

    GO - Molecular functioni

    • dCTP diphosphatase activity Source: UniProtKB
    • identical protein binding Source: IntAct
    • magnesium ion binding Source: UniProtKB
    • nucleoside-triphosphate diphosphatase activity Source: UniProtKB
    • pyrimidine deoxyribonucleotide binding Source: Ensembl

    GO - Biological processi

    • dCTP catabolic process Source: UniProtKB
    • DNA protection Source: UniProtKB
    • nucleoside triphosphate catabolic process Source: UniProtKB
    • protein homotetramerization Source: Ensembl

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiR-HSA-499943 Interconversion of nucleotide di- and triphosphates

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    dCTP pyrophosphatase 1Curated (EC:3.6.1.121 Publication)
    Alternative name(s):
    Deoxycytidine-triphosphatase 1
    Short name:
    dCTPase 1
    RS21C6Imported
    XTP3-transactivated gene A proteinImported
    Gene namesi
    Name:DCTPP1Imported
    Synonyms:XTP3TPAImported
    ORF Names:CDA03Imported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 16

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000179958.8
    HGNCiHGNC:28777 DCTPP1
    MIMi615840 gene
    neXtProtiNX_Q9H773

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi63E → Q: Loss of dCTP diphosphatase activity. 1 Publication1

    Organism-specific databases

    OpenTargetsiENSG00000179958
    PharmGKBiPA164718733

    Chemistry databases

    ChEMBLiCHEMBL3769292

    Polymorphism and mutation databases

    BioMutaiDCTPP1
    DMDMi74733624

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources
    ChainiPRO_00002917692 – 170dCTP pyrophosphatase 1Add BLAST169

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserineCombined sources1
    Modified residuei2PhosphoserineCombined sources1
    Modified residuei12PhosphothreonineCombined sources1
    Modified residuei85PhosphoserineCombined sources1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    EPDiQ9H773
    MaxQBiQ9H773
    PaxDbiQ9H773
    PeptideAtlasiQ9H773
    PRIDEiQ9H773
    ProteomicsDBi81085
    TopDownProteomicsiQ9H773

    PTM databases

    iPTMnetiQ9H773
    PhosphoSitePlusiQ9H773
    SwissPalmiQ9H773

    Expressioni

    Developmental stagei

    Expression increases during mitosis (at protein level).1 Publication

    Inductioni

    Up-regulated by an increase in cellular dCTP pool.1 Publication

    Gene expression databases

    BgeeiENSG00000179958
    ExpressionAtlasiQ9H773 baseline and differential
    GenevisibleiQ9H773 HS

    Organism-specific databases

    HPAiHPA002832

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Binary interactionsi

    Show more details

    GO - Molecular functioni

    • identical protein binding Source: IntAct

    Protein-protein interaction databases

    BioGridi122527, 35 interactors
    IntActiQ9H773, 12 interactors
    STRINGi9606.ENSP00000322524

    Chemistry databases

    BindingDBiQ9H773

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H773
    SMRiQ9H773
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni47 – 51Substrate bindingBy similarity5

    Phylogenomic databases

    eggNOGiENOG410IXWQ Eukaryota
    ENOG4111PYX LUCA
    GeneTreeiENSGT00390000017709
    HOGENOMiHOG000056700
    InParanoidiQ9H773
    KOiK16904
    OMAiELFQWKT
    OrthoDBiEOG091G0RYZ
    PhylomeDBiQ9H773
    TreeFamiTF300237

    Family and domain databases

    CDDicd11537 NTP-PPase_RS21-C6_like, 1 hit
    InterProiView protein in InterPro
    IPR025984 DCTPP
    PfamiView protein in Pfam
    PF12643 MazG-like, 1 hit
    PIRSFiPIRSF029826 UCP029826_pph, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9H773-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSVAGGEIRG DTGGEDTAAP GRFSFSPEPT LEDIRRLHAE FAAERDWEQF
    60 70 80 90 100
    HQPRNLLLAL VGEVGELAEL FQWKTDGEPG PQGWSPRERA ALQEELSDVL
    110 120 130 140 150
    IYLVALAARC RVDLPLAVLS KMDINRRRYP AHLARSSSRK YTELPHGAIS
    160 170
    EDQAVGPADI PCDSTGQTST
    Length:170
    Mass (Da):18,681
    Last modified:March 1, 2001 - v1
    Checksum:i23BC932D5461320B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY453409 mRNA Translation: AAR26724.1
    AF212242 mRNA Translation: AAK14927.1
    AF210430 mRNA Translation: AAK48422.1
    CR457335 mRNA Translation: CAG33616.1
    AK024843 mRNA Translation: BAB15025.1
    BC001344 mRNA Translation: AAH01344.1
    CCDSiCCDS10680.1
    RefSeqiNP_077001.1, NM_024096.1
    UniGeneiHs.632191

    Genome annotation databases

    EnsembliENST00000319285; ENSP00000322524; ENSG00000179958
    GeneIDi79077
    KEGGihsa:79077
    UCSCiuc002dyf.4 human

    Similar proteinsi

    Entry informationi

    Entry nameiDCTP1_HUMAN
    AccessioniPrimary (citable) accession number: Q9H773
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: March 1, 2001
    Last modified: June 20, 2018
    This is version 136 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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