Q9H773 (DCTP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
dCTP pyrophosphatase 1
EC=3.6.1.12

Alternative name(s):
Deoxycytidine-triphosphatase 1
Short name=dCTPase 1
RS21C6
XTP3-transactivated gene A protein

Gene names

Name:	DCTPP1
Synonyms:	XTP3TPA
ORF Names:	CDA03

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. Has a strong preference for modified dCTP. Activity is highest with 5-iodo-dCTP, followed by 5-bromo-dCTP, unmodified dCTP, 5-methyl-dCTP and 5-chloro-dCTP. Hydrolyzes 2-chloro-dATP and 2-hydroxy-dATP with lower efficiency, and has even lower activity with unmodified dATP, dTTP and dUTP (in vitro). Does not hydrolyze ATP, UTP, ITP, GTP, dADP, dCDP or dGTP. May protect DNA or RNA against the incorporation of non-canonical nucleotide triphosphates. May protect cells against inappropriate methylation of CpG islands by DNA methyltransferases By similarity.
Catalytic activity	dCTP + H2O = dCMP + diphosphate.
Cofactor	Magnesium. Probably binds two or three magnesium ions per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 EAR repeat.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside triphosphate catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dCTP diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	dCTP pyrophosphatase 1		PRO_0000291769
Regions
	Repeat	29 – 75	47	EAR
	Region	47 – 51	5	Substrate binding By similarity
Sites
	Metal binding	63	1	Magnesium By similarity
	Metal binding	66	1	Magnesium By similarity
	Metal binding	95	1	Magnesium By similarity
	Metal binding	98	1	Magnesium By similarity
	Binding site	38	1	Substrate By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	102	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	Phosphoserine Ref.8
	Modified residue	12	1	Phosphothreonine Ref.8
	Modified residue	85	1	Phosphoserine Ref.8
	Modified residue	138	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9H773 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 23BC932D5461320B	FASTA	170	18,681
	10 20 30 40 50 60 MSVAGGEIRG DTGGEDTAAP GRFSFSPEPT LEDIRRLHAE FAAERDWEQF HQPRNLLLAL 70 80 90 100 110 120 VGEVGELAEL FQWKTDGEPG PQGWSPRERA ALQEELSDVL IYLVALAARC RVDLPLAVLS 130 140 150 160 170 KMDINRRRYP AHLARSSSRK YTELPHGAIS EDQAVGPADI PCDSTGQTST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Screening and cloning of the target genes transactivated by XTP3 using a suppression subtractive hybridization technique." Wang C., Cheng J., Lang Z., Wu Y., Yang Y., Zhang L., Ji D. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A novel gene expressed in human pheochromocytoma." Li Y., Huang Q., Peng Y., Song H., Yu Y., Xu S., Ren S., Chen Z., Han Z. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pheochromocytoma.
[3]	Zhang J., Chen W.F., Wang H. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[7]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; THR-12 AND SER-85, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY453409 mRNA. Translation: AAR26724.1. AF212242 mRNA. Translation: AAK14927.1. AF210430 mRNA. Translation: AAK48422.1. CR457335 mRNA. Translation: CAG33616.1. AK024843 mRNA. Translation: BAB15025.1. BC001344 mRNA. Translation: AAH01344.1.
IPI	IPI00012197.
RefSeq	NP_077001.1. NM_024096.1.
UniGene	Hs.632191.
3D structure databases
ProteinModelPortal	Q9H773.
SMR	Q9H773. Positions 23-123.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H773. 5 interactions.
STRING	Q9H773.
PTM databases
PhosphoSite	Q9H773.
Polymorphism databases
DMDM	74733624.
Proteomic databases
PeptideAtlas	Q9H773.
PRIDE	Q9H773.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000319285; ENSP00000322524; ENSG00000179958.
GeneID	79077.
KEGG	hsa:79077.
UCSC	uc002dyf.1. human.
Organism-specific databases
CTD	79077.
GeneCards	GC16M030435.
H-InvDB	HIX0012958.
HGNC	HGNC:28777. DCTPP1.
HPA	HPA002832.
neXtProt	NX_Q9H773.
PharmGKB	PA164718733.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18276.
GeneTree	ENSGT00390000017709.
HOGENOM	HBG342270.
InParanoid	Q9H773.
OMA	WNQFHQP.
OrthoDB	EOG4X97JN.
PhylomeDB	Q9H773.
Gene expression databases
ArrayExpress	Q9H773.
Bgee	Q9H773.
Genevestigator	Q9H773.
Family and domain databases
InterPro	IPR009039. EAR. IPR004518. MazG_cat. [Graphical view]
Pfam	PF03819. MazG. 1 hit. [Graphical view]
PROSITE	PS50912. EAR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	67876.

Entry information

Entry name

DCTP1_HUMAN

Accession

Primary (citable) accession number: Q9H773

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.