ID   ASB8_HUMAN              Reviewed;         288 AA.
AC   Q9H765; A8K1P2; Q547Q2;
DT   28-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 177.
DE   RecName: Full=Ankyrin repeat and SOCS box protein 8;
DE            Short=ASB-8;
GN   Name=ASB8; ORFNames=PP14212;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=12559969; DOI=10.1016/s0006-291x(02)02971-6;
RA   Liu Y., Li J., Zhang F., Qin W., Yao G., He X., Xue P., Ge C., Wan D.,
RA   Gu J.;
RT   "Molecular cloning and characterization of the human ASB-8 gene encoding a
RT   novel member of ankyrin repeat and SOCS box containing protein family.";
RL   Biochem. Biophys. Res. Commun. 300:972-979(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Colon, and Hippocampus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Cervix;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: May be a substrate-recognition component of a SCF-like ECS
CC       (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex
CC       which mediates the ubiquitination and subsequent proteasomal
CC       degradation of target proteins. {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- INTERACTION:
CC       Q9H765; P40337-2: VHL; NbExp=3; IntAct=EBI-3942509, EBI-12157263;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12559969}.
CC   -!- TISSUE SPECIFICITY: Highest level of expression in skeletal muscle.
CC       Also expressed in heart, brain, placenta, liver, kidney and pancreas.
CC       {ECO:0000269|PubMed:12559969}.
CC   -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC       BC complex, an adapter module in different E3 ubiquitin-protein ligase
CC       complexes. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ankyrin SOCS box (ASB) family.
CC       {ECO:0000305}.
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DR   EMBL; AF464877; AAQ04830.1; -; mRNA.
DR   EMBL; AK024908; BAB15033.1; -; mRNA.
DR   EMBL; AK289957; BAF82646.1; -; mRNA.
DR   EMBL; BC001321; AAH01321.1; -; mRNA.
DR   CCDS; CCDS8761.1; -.
DR   RefSeq; NP_001306225.1; NM_001319296.1.
DR   RefSeq; NP_001306226.1; NM_001319297.1.
DR   RefSeq; NP_001306227.1; NM_001319298.1.
DR   RefSeq; NP_001306228.1; NM_001319299.1.
DR   RefSeq; NP_001306229.1; NM_001319300.1.
DR   RefSeq; NP_001306230.1; NM_001319301.1.
DR   RefSeq; NP_077000.1; NM_024095.4.
DR   AlphaFoldDB; Q9H765; -.
DR   SMR; Q9H765; -.
DR   BioGRID; 126614; 35.
DR   IntAct; Q9H765; 16.
DR   MINT; Q9H765; -.
DR   STRING; 9606.ENSP00000445622; -.
DR   GlyGen; Q9H765; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q9H765; -.
DR   PhosphoSitePlus; Q9H765; -.
DR   BioMuta; ASB8; -.
DR   DMDM; 29839757; -.
DR   EPD; Q9H765; -.
DR   jPOST; Q9H765; -.
DR   MassIVE; Q9H765; -.
DR   PaxDb; 9606-ENSP00000320893; -.
DR   PeptideAtlas; Q9H765; -.
DR   ProteomicsDB; 81083; -.
DR   Pumba; Q9H765; -.
DR   Antibodypedia; 1146; 228 antibodies from 25 providers.
DR   DNASU; 140461; -.
DR   Ensembl; ENST00000317697.8; ENSP00000320893.3; ENSG00000177981.11.
DR   Ensembl; ENST00000536549.5; ENSP00000445622.1; ENSG00000177981.11.
DR   GeneID; 140461; -.
DR   KEGG; hsa:140461; -.
DR   MANE-Select; ENST00000317697.8; ENSP00000320893.3; NM_024095.5; NP_077000.1.
DR   UCSC; uc001rrh.4; human.
DR   AGR; HGNC:17183; -.
DR   CTD; 140461; -.
DR   DisGeNET; 140461; -.
DR   GeneCards; ASB8; -.
DR   HGNC; HGNC:17183; ASB8.
DR   HPA; ENSG00000177981; Tissue enhanced (skeletal muscle, tongue).
DR   MIM; 615053; gene.
DR   neXtProt; NX_Q9H765; -.
DR   OpenTargets; ENSG00000177981; -.
DR   PharmGKB; PA25036; -.
DR   VEuPathDB; HostDB:ENSG00000177981; -.
DR   eggNOG; KOG0504; Eukaryota.
DR   GeneTree; ENSGT00940000159366; -.
DR   HOGENOM; CLU_000134_52_0_1; -.
DR   InParanoid; Q9H765; -.
DR   OMA; LHCACMA; -.
DR   OrthoDB; 4022749at2759; -.
DR   PhylomeDB; Q9H765; -.
DR   TreeFam; TF332452; -.
DR   PathwayCommons; Q9H765; -.
DR   Reactome; R-HSA-8951664; Neddylation.
DR   Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   SignaLink; Q9H765; -.
DR   SIGNOR; Q9H765; -.
DR   UniPathway; UPA00143; -.
DR   BioGRID-ORCS; 140461; 8 hits in 1194 CRISPR screens.
DR   ChiTaRS; ASB8; human.
DR   GenomeRNAi; 140461; -.
DR   Pharos; Q9H765; Tdark.
DR   PRO; PR:Q9H765; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; Q9H765; Protein.
DR   Bgee; ENSG00000177981; Expressed in gastrocnemius and 206 other cell types or tissues.
DR   ExpressionAtlas; Q9H765; baseline and differential.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   CDD; cd03727; SOCS_ASB8; 1.
DR   Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 2.
DR   Gene3D; 1.10.750.20; SOCS box; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR037332; ASB8_SOCS.
DR   InterPro; IPR001496; SOCS_box.
DR   InterPro; IPR036036; SOCS_box-like_dom_sf.
DR   PANTHER; PTHR24193:SF122; ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 23; 1.
DR   PANTHER; PTHR24193; ANKYRIN REPEAT PROTEIN; 1.
DR   Pfam; PF12796; Ank_2; 1.
DR   Pfam; PF13637; Ank_4; 1.
DR   Pfam; PF07525; SOCS_box; 1.
DR   SMART; SM00248; ANK; 4.
DR   SMART; SM00969; SOCS_box; 1.
DR   SUPFAM; SSF48403; Ankyrin repeat; 1.
DR   SUPFAM; SSF158235; SOCS box-like; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 4.
DR   PROSITE; PS50225; SOCS; 1.
DR   Genevisible; Q9H765; HS.
PE   1: Evidence at protein level;
KW   ANK repeat; Cytoplasm; Reference proteome; Repeat; Ubl conjugation pathway.
FT   CHAIN           1..288
FT                   /note="Ankyrin repeat and SOCS box protein 8"
FT                   /id="PRO_0000066938"
FT   REPEAT          52..81
FT                   /note="ANK 1"
FT   REPEAT          85..113
FT                   /note="ANK 2"
FT   REPEAT          117..146
FT                   /note="ANK 3"
FT   REPEAT          150..179
FT                   /note="ANK 4"
FT   DOMAIN          235..288
FT                   /note="SOCS box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
SQ   SEQUENCE   288 AA;  31642 MW;  B10FB7ADBDC3D3D6 CRC64;
     MSSSMWYIMQ SIQSKYSLSE RLIRTIAAIR SFPHDNVEDL IRGGADVNCT HGTLKPLHCA
     CMVSDADCVE LLLEKGAEVN ALDGYNRTAL HYAAEKDEAC VEVLLEYGAN PNALDGNRDT
     PLHWAAFKNN AECVRALLES GASVNALDYN NDTPLSWAAM KGNLESVSIL LDYGAEVRVI
     NLIGQTPISR LVALLVRGLG TEKEDSCFEL LHRAVGHFEL RKNGTMPREV ARDPQLCEKL
     TVLCSAPGTL KTLARYAVRR SLGLQYLPDA VKGLPLPASL KEYLLLLE
//