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Protein

Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66

Gene

NO66

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.By similarity3 Publications

Catalytic activityi

Protein N6,N(6)-dimethyl-L-lysine + 2-oxoglutarate + O2 = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO2.1 Publication
Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O2 = protein L-lysine + succinate + formaldehyde + CO2.1 Publication
L-histidine-[60S ribosomal protein L8] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-histidine-[60S ribosomal protein L8] + succinate + CO2.1 Publication

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi340Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi342Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi405Iron; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Dioxygenase, Oxidoreductase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170468-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 (EC:1.14.11.-, EC:1.14.11.27)
Alternative name(s):
60S ribosomal protein L8 histidine hydroxylase
Histone lysine demethylase NO66
Myc-associated protein with JmjC domain
Nucleolar protein 66
Short name:
hsNO66
Ribosomal oxygenase NO66
Short name:
ROX
Gene namesi
Name:NO66
Synonyms:C14orf169, MAPJD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20968. C14orf169.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi79697.
OpenTargetsiENSG00000170468.
PharmGKBiPA134919088.

Polymorphism and mutation databases

DMDMi284018103.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002646131 – 641Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66Add BLAST641

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei60PhosphoserineBy similarity1
Modified residuei63PhosphoserineBy similarity1
Modified residuei109PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H6W3.
MaxQBiQ9H6W3.
PeptideAtlasiQ9H6W3.
PRIDEiQ9H6W3.

PTM databases

iPTMnetiQ9H6W3.
PhosphoSitePlusiQ9H6W3.
SwissPalmiQ9H6W3.

Expressioni

Tissue specificityi

Widely expressed. Overexpressed in lung carcinomas.2 Publications

Gene expression databases

CleanExiHS_C14orf169.
GenevisibleiQ9H6W3. HS.

Interactioni

Subunit structurei

Interacts with SP7/OSX; the interaction is direct (By similarity). Interacts with MYC. Interacts with PHF19; leading to its recruitment to H3K36me3 sites.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Sp7Q8VI676EBI-2513645,EBI-7608836From a different organism.

Protein-protein interaction databases

BioGridi122818. 11 interactors.
DIPiDIP-53768N.
IntActiQ9H6W3. 11 interactors.
MINTiMINT-5009834.

Structurei

Secondary structure

1641
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi184 – 193Combined sources10
Helixi198 – 210Combined sources13
Helixi215 – 221Combined sources7
Turni222 – 225Combined sources4
Beta strandi228 – 230Combined sources3
Turni235 – 240Combined sources6
Helixi244 – 253Combined sources10
Turni259 – 261Combined sources3
Beta strandi262 – 268Combined sources7
Beta strandi271 – 274Combined sources4
Beta strandi278 – 280Combined sources3
Helixi283 – 291Combined sources9
Beta strandi295 – 299Combined sources5
Helixi301 – 303Combined sources3
Helixi306 – 319Combined sources14
Beta strandi324 – 330Combined sources7
Beta strandi332 – 335Combined sources4
Beta strandi343 – 353Combined sources11
Beta strandi355 – 359Combined sources5
Helixi365 – 367Combined sources3
Turni379 – 381Combined sources3
Beta strandi386 – 391Combined sources6
Beta strandi396 – 399Combined sources4
Beta strandi405 – 408Combined sources4
Beta strandi415 – 422Combined sources8
Helixi428 – 446Combined sources19
Helixi448 – 451Combined sources4
Helixi458 – 460Combined sources3
Helixi464 – 466Combined sources3
Helixi472 – 487Combined sources16
Helixi488 – 491Combined sources4
Helixi494 – 508Combined sources15
Helixi516 – 520Combined sources5
Helixi523 – 525Combined sources3
Beta strandi529 – 531Combined sources3
Beta strandi532 – 536Combined sources5
Beta strandi547 – 561Combined sources15
Beta strandi564 – 570Combined sources7
Beta strandi583 – 587Combined sources5
Helixi589 – 591Combined sources3
Helixi592 – 600Combined sources9
Turni601 – 603Combined sources3
Helixi608 – 610Combined sources3
Beta strandi612 – 615Combined sources4
Helixi616 – 628Combined sources13
Beta strandi632 – 636Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CCJX-ray2.15A/B/C/D183-641[»]
4CCKX-ray2.15A/B/C/D183-641[»]
4CCMX-ray2.51A/B183-641[»]
4CCNX-ray2.23A/B183-641[»]
4CCOX-ray2.30A/B183-641[»]
4DIQX-ray2.40A/B167-641[»]
4E4HX-ray2.28A/B/C/D183-641[»]
4Y33X-ray2.70A/B/C/D176-641[»]
4Y3OX-ray2.20A/B176-641[»]
4Y4RX-ray3.30A/B176-525[»]
A/B541-641[»]
ProteinModelPortaliQ9H6W3.
SMRiQ9H6W3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini294 – 439JmjCPROSITE-ProRule annotationAdd BLAST146

Sequence similaritiesi

Belongs to the ROX family. NO66 subfamily.Curated
Contains 1 JmjC domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000000083.
HOVERGENiHBG060021.
InParanoidiQ9H6W3.
KOiK16914.
OMAiFEWLIAP.
OrthoDBiEOG091G04K2.
PhylomeDBiQ9H6W3.

Family and domain databases

InterProiIPR003347. JmjC_dom.
[Graphical view]
PfamiPF08007. Cupin_4. 1 hit.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H6W3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGLQASAGP LRRGRPKRRR KPQPHSGSVL ALPLRSRKIR KQLRSVVSRM
60 70 80 90 100
AALRTQTLPS ENSEESRVES TADDLGDALP GGAAVAAVPD AARREPYGHL
110 120 130 140 150
GPAELLEASP AARSLQTPSA RLVPASAPPA RLVEVPAAPV RVVETSALLC
160 170 180 190 200
TAQHLAAVQS SGAPATASGP QVDNTGGEPA WDSPLRRVLA ELNRIPSSRR
210 220 230 240 250
RAARLFEWLI APMPPDHFYR RLWEREAVLV RRQDHTYYQG LFSTADLDSM
260 270 280 290 300
LRNEEVQFGQ HLDAARYING RRETLNPPGR ALPAAAWSLY QAGCSLRLLC
310 320 330 340 350
PQAFSTTVWQ FLAVLQEQFG SMAGSNVYLT PPNSQGFAPH YDDIEAFVLQ
360 370 380 390 400
LEGRKLWRVY RPRVPTEELA LTSSPNFSQD DLGEPVLQTV LEPGDLLYFP
410 420 430 440 450
RGFIHQAECQ DGVHSLHLTL STYQRNTWGD FLEAILPLAV QAAMEENVEF
460 470 480 490 500
RRGLPRDFMD YMGAQHSDSK DPRRTAFMEK VRVLVARLGH FAPVDAVADQ
510 520 530 540 550
RAKDFIHDSL PPVLTDRERA LSVYGLPIRW EAGEPVNVGA QLTTETEVHM
560 570 580 590 600
LQDGIARLVG EGGHLFLYYT VENSRVYHLE EPKCLEIYPQ QADAMELLLG
610 620 630 640
SYPEFVRVGD LPCDSVEDQL SLATTLYDKG LLLTKMPLAL N
Length:641
Mass (Da):71,086
Last modified:January 19, 2010 - v2
Checksum:iE66A4342B1D1BFF2
GO
Isoform 2 (identifier: Q9H6W3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-212: Missing.

Note: No experimental confirmation available.
Show »
Length:429
Mass (Da):48,687
Checksum:iF07631F3A3F8CDB7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti392E → G in BAG61814 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06242217K → R.3 PublicationsCorresponds to variant rs10144469dbSNPEnsembl.1
Natural variantiVAR_057819218F → S.Corresponds to variant rs758109dbSNPEnsembl.1
Natural variantiVAR_060191239Q → H.Corresponds to variant rs34970526dbSNPEnsembl.1
Natural variantiVAR_062423364V → A.3 PublicationsCorresponds to variant rs3813563dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0386631 – 212Missing in isoform 2. 1 PublicationAdd BLAST212

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390535 mRNA. Translation: AAR27292.1.
AK025455 mRNA. Translation: BAB15138.1.
AK299994 mRNA. Translation: BAG61814.1.
AC005280 Genomic DNA. No translation available.
BC011350 mRNA. Translation: AAH11350.1.
BC071954 mRNA. Translation: AAH71954.1.
CCDSiCCDS73660.1. [Q9H6W3-1]
RefSeqiNP_078920.2. NM_024644.4. [Q9H6W3-1]
UniGeneiHs.509916.

Genome annotation databases

EnsembliENST00000304061; ENSP00000477507; ENSG00000170468. [Q9H6W3-1]
GeneIDi79697.
KEGGihsa:79697.
UCSCiuc032bfz.2. human. [Q9H6W3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390535 mRNA. Translation: AAR27292.1.
AK025455 mRNA. Translation: BAB15138.1.
AK299994 mRNA. Translation: BAG61814.1.
AC005280 Genomic DNA. No translation available.
BC011350 mRNA. Translation: AAH11350.1.
BC071954 mRNA. Translation: AAH71954.1.
CCDSiCCDS73660.1. [Q9H6W3-1]
RefSeqiNP_078920.2. NM_024644.4. [Q9H6W3-1]
UniGeneiHs.509916.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CCJX-ray2.15A/B/C/D183-641[»]
4CCKX-ray2.15A/B/C/D183-641[»]
4CCMX-ray2.51A/B183-641[»]
4CCNX-ray2.23A/B183-641[»]
4CCOX-ray2.30A/B183-641[»]
4DIQX-ray2.40A/B167-641[»]
4E4HX-ray2.28A/B/C/D183-641[»]
4Y33X-ray2.70A/B/C/D176-641[»]
4Y3OX-ray2.20A/B176-641[»]
4Y4RX-ray3.30A/B176-525[»]
A/B541-641[»]
ProteinModelPortaliQ9H6W3.
SMRiQ9H6W3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122818. 11 interactors.
DIPiDIP-53768N.
IntActiQ9H6W3. 11 interactors.
MINTiMINT-5009834.

PTM databases

iPTMnetiQ9H6W3.
PhosphoSitePlusiQ9H6W3.
SwissPalmiQ9H6W3.

Polymorphism and mutation databases

DMDMi284018103.

Proteomic databases

EPDiQ9H6W3.
MaxQBiQ9H6W3.
PeptideAtlasiQ9H6W3.
PRIDEiQ9H6W3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304061; ENSP00000477507; ENSG00000170468. [Q9H6W3-1]
GeneIDi79697.
KEGGihsa:79697.
UCSCiuc032bfz.2. human. [Q9H6W3-1]

Organism-specific databases

CTDi79697.
DisGeNETi79697.
GeneCardsiC14orf169.
H-InvDBHIX0011794.
HGNCiHGNC:20968. C14orf169.
MIMi611919. gene.
neXtProtiNX_Q9H6W3.
OpenTargetsiENSG00000170468.
PharmGKBiPA134919088.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000000083.
HOVERGENiHBG060021.
InParanoidiQ9H6W3.
KOiK16914.
OMAiFEWLIAP.
OrthoDBiEOG091G04K2.
PhylomeDBiQ9H6W3.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170468-MONOMER.

Miscellaneous databases

GenomeRNAii79697.
PROiQ9H6W3.
SOURCEiSearch...

Gene expression databases

CleanExiHS_C14orf169.
GenevisibleiQ9H6W3. HS.

Family and domain databases

InterProiIPR003347. JmjC_dom.
[Graphical view]
PfamiPF08007. Cupin_4. 1 hit.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNO66_HUMAN
AccessioniPrimary (citable) accession number: Q9H6W3
Secondary accession number(s): B4DT02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: January 19, 2010
Last modified: November 2, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.