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Protein

Epithelial splicing regulatory protein 2

Gene

ESRP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.1 Publication

GO - Molecular functioni

  • mRNA binding Source: UniProtKB
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • regulation of RNA splicing Source: UniProtKB
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Epithelial splicing regulatory protein 2
Alternative name(s):
RNA-binding motif protein 35B
RNA-binding protein 35B
Gene namesi
Name:ESRP2
Synonyms:RBM35B
ORF Names:PP7059
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:26152. ESRP2.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164719361.

Polymorphism and mutation databases

BioMutaiESRP2.
DMDMi74761482.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Epithelial splicing regulatory protein 2PRO_0000273050Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei83 – 831PhosphoserineBy similarity
Modified residuei573 – 5731PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H6T0.
MaxQBiQ9H6T0.
PaxDbiQ9H6T0.
PRIDEiQ9H6T0.

PTM databases

iPTMnetiQ9H6T0.
PhosphoSiteiQ9H6T0.

Expressioni

Tissue specificityi

Epithelial cell-specific.1 Publication

Inductioni

Down-regulated during the epithelial-to-mesenchymal transition (EMT).1 Publication

Gene expression databases

BgeeiQ9H6T0.
CleanExiHS_RBM35B.
ExpressionAtlasiQ9H6T0. baseline and differential.
GenevisibleiQ9H6T0. HS.

Organism-specific databases

HPAiHPA048597.
HPA048618.

Interactioni

Protein-protein interaction databases

BioGridi123061. 7 interactions.
IntActiQ9H6T0. 4 interactions.
STRINGi9606.ENSP00000418748.

Structurei

3D structure databases

ProteinModelPortaliQ9H6T0.
SMRiQ9H6T0. Positions 259-345, 347-452, 477-558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini257 – 35397RRM 1Add
BLAST
Domaini358 – 43881RRM 2Add
BLAST
Domaini475 – 55581RRM 3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 2725Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the ESRP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1365. Eukaryota.
ENOG410XPZU. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000112401.
InParanoidiQ9H6T0.
KOiK14947.
OMAiREFHVQH.
OrthoDBiEOG76QFGV.
PhylomeDBiQ9H6T0.
TreeFamiTF316157.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012337. RNaseH-like_dom.
IPR000504. RRM_dom.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
SSF54928. SSF54928. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H6T0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTPPPPPPPP PGPDPAADPA ADPCPWPGSL VVLFGATAGA LGRDLGSDET
60 70 80 90 100
DLILLVWQVV EPRSRQVGTL HKSLVRAEAA ALSTQCREAS GLSADSLARA
110 120 130 140 150
EPLDKVLQQF SQLVNGDVAL LGGGPYMLCT DGQQLLRQVL HPEASRKNLV
160 170 180 190 200
LPDMFFSFYD LRREFHMQHP STCPARDLTV ATMAQGLGLE TDATEDDFGV
210 220 230 240 250
WEVKTMVAVI LHLLKEPSSQ LFSKPEVIKQ KYETGPCSDS TVPCPYSSKA
260 270 280 290 300
DVVDSETVVR ARGLPWQSSD QDVARFFKGL NVARGGVALC LNAQGRRNGE
310 320 330 340 350
ALIRFVDSEQ RDLALQRHKH HMGVRYIEVY KATGEEFVKI AGGTSLEVAR
360 370 380 390 400
FLSREDQVIL RLRGLPFSAG PTDVLGFLGP ECPVTGGTEG LLFVRHPDGR
410 420 430 440 450
PTGDAFALFA CEELAQAALR RHKGMLGKRY IELFRSTAAE VQQVLNRYAS
460 470 480 490 500
GPLLPTLTAP LLPIPFPLAP GTGRDCVRLR GLPYTATIED ILSFLGEAAA
510 520 530 540 550
DIRPHGVHMV LNQQGRPSGD AFIQMTSAER ALAAAQRCHK KVMKERYVEV
560 570 580 590 600
VPCSTEEMSR VLMGGTLGRS GMSPPPCKLP CLSPPTYTTF QATPTLIPTE
610 620 630 640 650
TAALYPSSAL LPAARVPAAP TPVAYYPGPA TQLYLNYTAY YPSPPVSPTT
660 670 680 690 700
VGYLTTPTAA LASAPTSVLS QSGALVRMQG VPYTAGMKDL LSVFQAYQLP
710 720
ADDYTSLMPV GDPPRTVLQA PKEWVCL
Length:727
Mass (Da):78,401
Last modified:March 1, 2001 - v1
Checksum:i8831BA8284D6BD60
GO
Isoform 2 (identifier: Q9H6T0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     238-247: Missing.

Note: No experimental confirmation available.
Show »
Length:717
Mass (Da):77,364
Checksum:i4AB000FD043A92BC
GO

Sequence cautioni

The sequence AAL55761.1 differs from that shown. Reason: Frameshift at position 370. Curated
The sequence BAB15275.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti586 – 5861T → I in BAB15275 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti111 – 1111S → L.
Corresponds to variant rs12597504 [ dbSNP | Ensembl ].
VAR_030074
Natural varianti528 – 5281A → V.
Corresponds to variant rs3743738 [ dbSNP | Ensembl ].
VAR_030075
Natural varianti627 – 6271P → S.
Corresponds to variant rs36054935 [ dbSNP | Ensembl ].
VAR_057245

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei238 – 24710Missing in isoform 2. 1 PublicationVSP_022474

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK025571 mRNA. Translation: BAB15173.1.
AK025901 mRNA. Translation: BAB15275.1. Different initiation.
BC030146 mRNA. Translation: AAH30146.1.
AF289577 mRNA. Translation: AAL55761.1. Frameshift.
CCDSiCCDS10863.1. [Q9H6T0-2]
RefSeqiNP_079215.2. NM_024939.2. [Q9H6T0-2]
UniGeneiHs.436585.
Hs.592053.

Genome annotation databases

EnsembliENST00000473183; ENSP00000418748; ENSG00000103067. [Q9H6T0-2]
ENST00000565858; ENSP00000454554; ENSG00000103067. [Q9H6T0-1]
GeneIDi80004.
KEGGihsa:80004.
UCSCiuc002evq.2. human. [Q9H6T0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK025571 mRNA. Translation: BAB15173.1.
AK025901 mRNA. Translation: BAB15275.1. Different initiation.
BC030146 mRNA. Translation: AAH30146.1.
AF289577 mRNA. Translation: AAL55761.1. Frameshift.
CCDSiCCDS10863.1. [Q9H6T0-2]
RefSeqiNP_079215.2. NM_024939.2. [Q9H6T0-2]
UniGeneiHs.436585.
Hs.592053.

3D structure databases

ProteinModelPortaliQ9H6T0.
SMRiQ9H6T0. Positions 259-345, 347-452, 477-558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123061. 7 interactions.
IntActiQ9H6T0. 4 interactions.
STRINGi9606.ENSP00000418748.

PTM databases

iPTMnetiQ9H6T0.
PhosphoSiteiQ9H6T0.

Polymorphism and mutation databases

BioMutaiESRP2.
DMDMi74761482.

Proteomic databases

EPDiQ9H6T0.
MaxQBiQ9H6T0.
PaxDbiQ9H6T0.
PRIDEiQ9H6T0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000473183; ENSP00000418748; ENSG00000103067. [Q9H6T0-2]
ENST00000565858; ENSP00000454554; ENSG00000103067. [Q9H6T0-1]
GeneIDi80004.
KEGGihsa:80004.
UCSCiuc002evq.2. human. [Q9H6T0-1]

Organism-specific databases

CTDi80004.
GeneCardsiESRP2.
HGNCiHGNC:26152. ESRP2.
HPAiHPA048597.
HPA048618.
MIMi612960. gene.
neXtProtiNX_Q9H6T0.
PharmGKBiPA164719361.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1365. Eukaryota.
ENOG410XPZU. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000112401.
InParanoidiQ9H6T0.
KOiK14947.
OMAiREFHVQH.
OrthoDBiEOG76QFGV.
PhylomeDBiQ9H6T0.
TreeFamiTF316157.

Miscellaneous databases

ChiTaRSiESRP2. human.
GenomeRNAii80004.
NextBioi70079.
PROiQ9H6T0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H6T0.
CleanExiHS_RBM35B.
ExpressionAtlasiQ9H6T0. baseline and differential.
GenevisibleiQ9H6T0. HS.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012337. RNaseH-like_dom.
IPR000504. RRM_dom.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
SSF54928. SSF54928. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Hepatoma.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Placenta.
  3. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 248-727.
  4. "ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing."
    Warzecha C.C., Sato T.K., Nabet B., Hogenesch J.B., Carstens R.P.
    Mol. Cell 33:591-601(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING, TISSUE SPECIFICITY, INDUCTION.
  5. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-573, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiESRP2_HUMAN
AccessioniPrimary (citable) accession number: Q9H6T0
Secondary accession number(s): Q8N6H8, Q8WZ15, Q9H6I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.