Q9H6S3 (ES8L2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 2
Short name=EPS8-like protein 2

Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 2
Short name=EPS8-related protein 2

Gene names

Name:	EPS8L2
Synonyms:	EPS8R2
ORF Names:	PP13181

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. Ref.6
Subunit structure	Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin. Ref.6
Subcellular location	Cytoplasm Ref.6.
Tissue specificity	Detected in fibroblasts and placenta. Ref.6
Sequence similarities	Belongs to the EPS8 family. Contains 1 PID domain. Contains 1 SH3 domain.
Sequence caution	The sequence AAL55838.1 differs from that shown. Reason: Frameshift at position 593. The sequence BAB15180.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H6S3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H6S3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-388: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 715

715

Epidermal growth factor receptor kinase substrate 8-like protein 2

PRO_0000239084

Regions

Domain

46 – 202

157

PID

Domain

492 – 551

60

SH3

Amino acid modifications

Modified residue

240

1

Phosphoserine By similarity

Modified residue

449

1

Phosphoserine Ref.7

Modified residue

469

1

Phosphothreonine Ref.8

Modified residue

480

1

Phosphoserine By similarity

Modified residue

570

1

Phosphoserine Ref.8

Natural variations

Alternative sequence

1 – 388

388

Missing in isoform 2.

VSP_019092

Experimental info

Sequence conflict

146

1

S → N in BAD96623. Ref.4

Sequence conflict

198

1

I → F in BAB15180. Ref.3

Sequence conflict

589

1

H → R in BAB15180. Ref.3

Sequence conflict

620

1

V → A in BAB15180. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

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.

.

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.

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715

Helix Strand Turn

Details...

Beta strand

506 – 510

5

Beta strand

518 – 523

6

Beta strand

525 – 532

8

Beta strand

538 – 542

5

Turn

543 – 545

3

Helix

630 – 640

11

Helix

646 – 649

4

Helix

655 – 659

5

Helix

663 – 670

8

Turn

671 – 673

3

Helix

674 – 689

16

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: DAB07744B04CFEE2	FASTA	715	80,621
	10 20 30 40 50 60 MSQSGAVSCC PGATNGSLGR SDGVAKMSPK DLFEQRKKYS NSNVIMHETS QYHVQHLATF 70 80 90 100 110 120 IMDKSEAITS VDDAIRKLVQ LSSKEKIWTQ EMLLQVNDQS LRLLDIESQE ELEDFPLPTV 130 140 150 160 170 180 QRSQTVLNQL RYPSVLLLVC QDSEQSKPDV HFFHCDEVEA ELVHEDIESA LADCRLGKKM 190 200 210 220 230 240 RPQTLKGHQE KIRQRQSILP PPQGPAPIPF QHRGGDSPEA KNRVGPQVPL SEPGFRRRES 250 260 270 280 290 300 QEEPRAVLAQ KIEKETQILN CALDDIEWFV ARLQKAAEAF KQLNQRKKGK KKGKKAPAEG 310 320 330 340 350 360 VLTLRARPPS EGEFIDCFQK IKLAINLLAK LQKHIQNPSA AELVHFLFGP LDLIVNTCSG 370 380 390 400 410 420 PDIARSVSCP LLSRDAVDFL RGHLVPKEMS LWESLGESWM RPRSEWPREP QVPLYVPKFH 430 440 450 460 470 480 SGWEPPVDVL QEAPWEVEGL ASAPIEEVSP VSRQSIRNSQ KHSPTSEPTP PGDALPPVSS 490 500 510 520 530 540 PHTHRGYQPT PAMAKYVKIL YDFTARNANE LSVLKDEVLE VLEDGRQWWK LRSRSGQAGY 550 560 570 580 590 600 VPCNILGEAR PEDAGAPFEQ AGQKYWGPAS PTHKLPPSFP GNKDELMQHM DEVNDELIRK 610 620 630 640 650 660 ISNIRAQPQR HFRVERSQPV SQPLTYESGP DEVRAWLEAK AFSPRIVENL GILTGPQLFS 670 680 690 700 710 LNKEELKKVC GEEGVRVYSQ LTMQKAFLEK QQSGSELEEL MNKFHSMNQR RGEDS
	Isoform 2 [UniParc]. Checksum: 9D933D44E34CC320	FASTA	327	37,113
	10 20 30 40 50 60 MSLWESLGES WMRPRSEWPR EPQVPLYVPK FHSGWEPPVD VLQEAPWEVE GLASAPIEEV 70 80 90 100 110 120 SPVSRQSIRN SQKHSPTSEP TPPGDALPPV SSPHTHRGYQ PTPAMAKYVK ILYDFTARNA 130 140 150 160 170 180 NELSVLKDEV LEVLEDGRQW WKLRSRSGQA GYVPCNILGE ARPEDAGAPF EQAGQKYWGP 190 200 210 220 230 240 ASPTHKLPPS FPGNKDELMQ HMDEVNDELI RKISNIRAQP QRHFRVERSQ PVSQPLTYES 250 260 270 280 290 300 GPDEVRAWLE AKAFSPRIVE NLGILTGPQL FSLNKEELKK VCGEEGVRVY SQLTMQKAFL 310 320 EKQQSGSELE ELMNKFHSMN QRRGEDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure." Tocchetti A., Confalonieri S., Scita G., Di Fiore P.P., Betsholtz C. Genomics 81:234-244(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain cortex and Placenta.
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Prostate.
[6]	"The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway." Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G. Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH ABI1 AND SOS1, INTERACTION WITH ABI1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-449, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-469 AND SER-570, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"solution structure of the SH3 domain from human epidermal growth factor receptor pathway substrate 8-like protein." RIKEN structural genomics initiative (RSGI) Submitted (JUL-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 495-549.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY074929 mRNA. Translation: AAL76118.1.
AF318331 mRNA. Translation: AAL55838.1. Frameshift.
AK025588 mRNA. Translation: BAB15180.1. Different initiation.
AK025824 mRNA. Translation: BAB15248.1.
AK027765 mRNA. Translation: BAB55354.1.
AK094539 mRNA. Translation: BAG52883.1.
AK222903 mRNA. Translation: BAD96623.1.
BC080636 mRNA. Translation: AAH80636.1.
BC093878 mRNA. Translation: AAH93878.1.
BC101481 mRNA. Translation: AAI01482.1.

IPI

IPI00414315.
IPI00759759.

RefSeq

NP_073609.2. NM_022772.3.

UniGene

Hs.55016.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WWU	NMR	-	A	612-697	[»]
1WXB	NMR	-	A	495-549	[»]

ProteinModelPortal

Q9H6S3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9H6S3. 1 interaction.

MINT

MINT-5002000.

STRING

9606.ENSP00000320828.

PTM databases

PhosphoSite

Q9H6S3.

Polymorphism databases

DMDM

108864726.

Proteomic databases

PaxDb

Q9H6S3.

PRIDE

Q9H6S3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000318562; ENSP00000320828; ENSG00000177106.
ENST00000530636; ENSP00000436035; ENSG00000177106.
ENST00000533256; ENSP00000435585; ENSG00000177106.

GeneID

64787.

KEGG

hsa:64787.

UCSC

uc001lqt.3. human.
uc001lqw.3. human.

Organism-specific databases

CTD

64787.

GeneCards

GC11P000696.

HGNC

HGNC:21296. EPS8L2.

HPA

HPA041143.

MIM

614988. gene.

neXtProt

NX_Q9H6S3.

PharmGKB

PA134981048.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG263108.

HOVERGEN

HBG003090.

InParanoid

Q9H6S3.

OrthoDB

EOG4X0MRS.

PhylomeDB

Q9H6S3.

Gene expression databases

ArrayExpress

Q9H6S3.

Bgee

Q9H6S3.

CleanEx

HS_EPS8L2.

Genevestigator

Q9H6S3.

GermOnline

ENSG00000177106. Homo sapiens.

Family and domain databases

Gene3D

2.30.29.30. 1 hit.

InterPro

IPR011993. PH_like_dom.
IPR013625. PTB.
IPR006020. PTyr_interaction_dom.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

SMART

SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

EPS8L2. human.

EvolutionaryTrace

Q9H6S3.

GenomeRNAi

64787.

NextBio

66840.

SOURCE

Search...

Entry information

Entry name

ES8L2_HUMAN

Accession

Primary (citable) accession number: Q9H6S3
Secondary accession number(s): B3KSX1 , Q53GM8, Q8WYW7, Q96K06, Q9H6K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	May 30, 2006
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.