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Protein

Epidermal growth factor receptor kinase substrate 8-like protein 2

Gene

EPS8L2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

GO - Biological processi

  • positive regulation of GTPase activity Source: GOC
  • positive regulation of ruffle assembly Source: UniProtKB
  • regulation of Rho protein signal transduction Source: UniProtKB
  • Rho protein signal transduction Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000177106-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 2
Short name:
EPS8-like protein 2
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 2
Short name:
EPS8-related protein 2
Gene namesi
Name:EPS8L2
Synonyms:EPS8R2
ORF Names:PP13181
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:21296. EPS8L2.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: HPA
  • extracellular exosome Source: UniProtKB
  • plasma membrane Source: HPA
  • protein complex Source: UniProtKB
  • ruffle membrane Source: UniProtKB
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi64787.
OpenTargetsiENSG00000177106.
PharmGKBiPA134981048.

Polymorphism and mutation databases

BioMutaiEPS8L2.
DMDMi108864726.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002390841 – 715Epidermal growth factor receptor kinase substrate 8-like protein 2Add BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei240PhosphoserineCombined sources1
Modified residuei303PhosphothreonineBy similarity1
Modified residuei449PhosphoserineCombined sources1
Modified residuei469PhosphothreonineCombined sources1
Modified residuei570PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H6S3.
MaxQBiQ9H6S3.
PaxDbiQ9H6S3.
PeptideAtlasiQ9H6S3.
PRIDEiQ9H6S3.

PTM databases

iPTMnetiQ9H6S3.
PhosphoSitePlusiQ9H6S3.

Expressioni

Tissue specificityi

Detected in fibroblasts and placenta.1 Publication

Gene expression databases

BgeeiENSG00000177106.
CleanExiHS_EPS8L2.
ExpressionAtlasiQ9H6S3. baseline and differential.
GenevisibleiQ9H6S3. HS.

Organism-specific databases

HPAiHPA041143.

Interactioni

Subunit structurei

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin.1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi122297. 13 interactors.
IntActiQ9H6S3. 9 interactors.
MINTiMINT-5002000.
STRINGi9606.ENSP00000320828.

Structurei

Secondary structure

1715
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi506 – 510Combined sources5
Beta strandi518 – 523Combined sources6
Beta strandi525 – 532Combined sources8
Beta strandi538 – 542Combined sources5
Turni543 – 545Combined sources3
Helixi630 – 640Combined sources11
Helixi646 – 649Combined sources4
Helixi655 – 659Combined sources5
Helixi663 – 670Combined sources8
Turni671 – 673Combined sources3
Helixi674 – 689Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WWUNMR-A612-697[»]
1WXBNMR-A495-549[»]
ProteinModelPortaliQ9H6S3.
SMRiQ9H6S3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H6S3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 202PIDPROSITE-ProRule annotationAdd BLAST157
Domaini492 – 551SH3PROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Belongs to the EPS8 family.Curated
Contains 1 PID domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3557. Eukaryota.
ENOG410XT9R. LUCA.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ9H6S3.
KOiK17277.
OMAiFRNSPKH.
OrthoDBiEOG091G023T.
PhylomeDBiQ9H6S3.
TreeFamiTF313069.

Family and domain databases

CDDicd01210. PTB_EPS8. 1 hit.
Gene3Di2.30.29.30. 1 hit.
InterProiIPR033928. EPS8_PTB.
IPR011993. PH_dom-like.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H6S3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQSGAVSCC PGATNGSLGR SDGVAKMSPK DLFEQRKKYS NSNVIMHETS
60 70 80 90 100
QYHVQHLATF IMDKSEAITS VDDAIRKLVQ LSSKEKIWTQ EMLLQVNDQS
110 120 130 140 150
LRLLDIESQE ELEDFPLPTV QRSQTVLNQL RYPSVLLLVC QDSEQSKPDV
160 170 180 190 200
HFFHCDEVEA ELVHEDIESA LADCRLGKKM RPQTLKGHQE KIRQRQSILP
210 220 230 240 250
PPQGPAPIPF QHRGGDSPEA KNRVGPQVPL SEPGFRRRES QEEPRAVLAQ
260 270 280 290 300
KIEKETQILN CALDDIEWFV ARLQKAAEAF KQLNQRKKGK KKGKKAPAEG
310 320 330 340 350
VLTLRARPPS EGEFIDCFQK IKLAINLLAK LQKHIQNPSA AELVHFLFGP
360 370 380 390 400
LDLIVNTCSG PDIARSVSCP LLSRDAVDFL RGHLVPKEMS LWESLGESWM
410 420 430 440 450
RPRSEWPREP QVPLYVPKFH SGWEPPVDVL QEAPWEVEGL ASAPIEEVSP
460 470 480 490 500
VSRQSIRNSQ KHSPTSEPTP PGDALPPVSS PHTHRGYQPT PAMAKYVKIL
510 520 530 540 550
YDFTARNANE LSVLKDEVLE VLEDGRQWWK LRSRSGQAGY VPCNILGEAR
560 570 580 590 600
PEDAGAPFEQ AGQKYWGPAS PTHKLPPSFP GNKDELMQHM DEVNDELIRK
610 620 630 640 650
ISNIRAQPQR HFRVERSQPV SQPLTYESGP DEVRAWLEAK AFSPRIVENL
660 670 680 690 700
GILTGPQLFS LNKEELKKVC GEEGVRVYSQ LTMQKAFLEK QQSGSELEEL
710
MNKFHSMNQR RGEDS
Length:715
Mass (Da):80,621
Last modified:May 30, 2006 - v2
Checksum:iDAB07744B04CFEE2
GO
Isoform 2 (identifier: Q9H6S3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-388: Missing.

Show »
Length:327
Mass (Da):37,113
Checksum:i9D933D44E34CC320
GO
Isoform 3 (identifier: Q9H6S3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-108: S → SQSAQTPGASRVRAMYP

Note: No experimental confirmation available.
Show »
Length:731
Mass (Da):82,323
Checksum:i9D355BAFCF33601B
GO

Sequence cautioni

The sequence AAL55838 differs from that shown. Reason: Frameshift at position 593.Curated
The sequence BAB15180 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146S → N in BAD96623 (Ref. 4) Curated1
Sequence conflicti198I → F in BAB15180 (PubMed:14702039).Curated1
Sequence conflicti589H → R in BAB15180 (PubMed:14702039).Curated1
Sequence conflicti620V → A in BAB15180 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0190921 – 388Missing in isoform 2. 2 PublicationsAdd BLAST388
Alternative sequenceiVSP_054144108S → SQSAQTPGASRVRAMYP in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074929 mRNA. Translation: AAL76118.1.
AF318331 mRNA. Translation: AAL55838.1. Frameshift.
AK025588 mRNA. Translation: BAB15180.1. Different initiation.
AK025824 mRNA. Translation: BAB15248.1.
AK027765 mRNA. Translation: BAB55354.1.
AK094539 mRNA. Translation: BAG52883.1.
AK222903 mRNA. Translation: BAD96623.1.
BC080636 mRNA. Translation: AAH80636.1.
BC093878 mRNA. Translation: AAH93878.1.
BC101481 mRNA. Translation: AAI01482.1.
BC143242 mRNA. Translation: AAI43243.1.
AC131934 Genomic DNA. No translation available.
AP006621 Genomic DNA. No translation available.
CCDSiCCDS31328.1. [Q9H6S3-1]
RefSeqiNP_073609.2. NM_022772.3. [Q9H6S3-1]
XP_016873619.1. XM_017018130.1. [Q9H6S3-1]
XP_016873620.1. XM_017018131.1. [Q9H6S3-1]
XP_016873621.1. XM_017018132.1. [Q9H6S3-1]
UniGeneiHs.55016.

Genome annotation databases

EnsembliENST00000318562; ENSP00000320828; ENSG00000177106. [Q9H6S3-1]
ENST00000526198; ENSP00000436230; ENSG00000177106. [Q9H6S3-3]
ENST00000530636; ENSP00000436035; ENSG00000177106. [Q9H6S3-1]
ENST00000533256; ENSP00000435585; ENSG00000177106. [Q9H6S3-1]
ENST00000614442; ENSP00000480201; ENSG00000177106. [Q9H6S3-3]
GeneIDi64787.
KEGGihsa:64787.
UCSCiuc001lqt.4. human. [Q9H6S3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074929 mRNA. Translation: AAL76118.1.
AF318331 mRNA. Translation: AAL55838.1. Frameshift.
AK025588 mRNA. Translation: BAB15180.1. Different initiation.
AK025824 mRNA. Translation: BAB15248.1.
AK027765 mRNA. Translation: BAB55354.1.
AK094539 mRNA. Translation: BAG52883.1.
AK222903 mRNA. Translation: BAD96623.1.
BC080636 mRNA. Translation: AAH80636.1.
BC093878 mRNA. Translation: AAH93878.1.
BC101481 mRNA. Translation: AAI01482.1.
BC143242 mRNA. Translation: AAI43243.1.
AC131934 Genomic DNA. No translation available.
AP006621 Genomic DNA. No translation available.
CCDSiCCDS31328.1. [Q9H6S3-1]
RefSeqiNP_073609.2. NM_022772.3. [Q9H6S3-1]
XP_016873619.1. XM_017018130.1. [Q9H6S3-1]
XP_016873620.1. XM_017018131.1. [Q9H6S3-1]
XP_016873621.1. XM_017018132.1. [Q9H6S3-1]
UniGeneiHs.55016.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WWUNMR-A612-697[»]
1WXBNMR-A495-549[»]
ProteinModelPortaliQ9H6S3.
SMRiQ9H6S3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122297. 13 interactors.
IntActiQ9H6S3. 9 interactors.
MINTiMINT-5002000.
STRINGi9606.ENSP00000320828.

PTM databases

iPTMnetiQ9H6S3.
PhosphoSitePlusiQ9H6S3.

Polymorphism and mutation databases

BioMutaiEPS8L2.
DMDMi108864726.

Proteomic databases

EPDiQ9H6S3.
MaxQBiQ9H6S3.
PaxDbiQ9H6S3.
PeptideAtlasiQ9H6S3.
PRIDEiQ9H6S3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000318562; ENSP00000320828; ENSG00000177106. [Q9H6S3-1]
ENST00000526198; ENSP00000436230; ENSG00000177106. [Q9H6S3-3]
ENST00000530636; ENSP00000436035; ENSG00000177106. [Q9H6S3-1]
ENST00000533256; ENSP00000435585; ENSG00000177106. [Q9H6S3-1]
ENST00000614442; ENSP00000480201; ENSG00000177106. [Q9H6S3-3]
GeneIDi64787.
KEGGihsa:64787.
UCSCiuc001lqt.4. human. [Q9H6S3-1]

Organism-specific databases

CTDi64787.
DisGeNETi64787.
GeneCardsiEPS8L2.
HGNCiHGNC:21296. EPS8L2.
HPAiHPA041143.
MIMi614988. gene.
neXtProtiNX_Q9H6S3.
OpenTargetsiENSG00000177106.
PharmGKBiPA134981048.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3557. Eukaryota.
ENOG410XT9R. LUCA.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ9H6S3.
KOiK17277.
OMAiFRNSPKH.
OrthoDBiEOG091G023T.
PhylomeDBiQ9H6S3.
TreeFamiTF313069.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000177106-MONOMER.

Miscellaneous databases

ChiTaRSiEPS8L2. human.
EvolutionaryTraceiQ9H6S3.
GeneWikiiEPS8L2.
GenomeRNAii64787.
PROiQ9H6S3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000177106.
CleanExiHS_EPS8L2.
ExpressionAtlasiQ9H6S3. baseline and differential.
GenevisibleiQ9H6S3. HS.

Family and domain databases

CDDicd01210. PTB_EPS8. 1 hit.
Gene3Di2.30.29.30. 1 hit.
InterProiIPR033928. EPS8_PTB.
IPR011993. PH_dom-like.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiES8L2_HUMAN
AccessioniPrimary (citable) accession number: Q9H6S3
Secondary accession number(s): B3KSX1
, B7ZKL3, Q53GM8, Q8WYW7, Q96K06, Q9H6K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.