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Q9H6S3 (ES8L2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 2

Short name=EPS8-like protein 2
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 2
Short name=EPS8-related protein 2
Gene names
Name:EPS8L2
Synonyms:EPS8R2
ORF Names:PP13181
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. Ref.7

Subunit structure

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin. Ref.7

Subcellular location

Cytoplasm Ref.7.

Tissue specificity

Detected in fibroblasts and placenta. Ref.7

Sequence similarities

Belongs to the EPS8 family.

Contains 1 PID domain.

Contains 1 SH3 domain.

Sequence caution

The sequence AAL55838.1 differs from that shown. Reason: Frameshift at position 593.

The sequence BAB15180.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H6S3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H6S3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-388: Missing.
Isoform 3 (identifier: Q9H6S3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     108-108: S → SQSAQTPGASRVRAMYP
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 715715Epidermal growth factor receptor kinase substrate 8-like protein 2
PRO_0000239084

Regions

Domain46 – 202157PID
Domain492 – 55160SH3

Amino acid modifications

Modified residue3031Phosphothreonine By similarity
Modified residue4491Phosphoserine Ref.8
Modified residue4691Phosphothreonine Ref.9
Modified residue5701Phosphoserine Ref.9

Natural variations

Alternative sequence1 – 388388Missing in isoform 2.
VSP_019092
Alternative sequence1081S → SQSAQTPGASRVRAMYP in isoform 3.
VSP_054144

Experimental info

Sequence conflict1461S → N in BAD96623. Ref.4
Sequence conflict1981I → F in BAB15180. Ref.3
Sequence conflict5891H → R in BAB15180. Ref.3
Sequence conflict6201V → A in BAB15180. Ref.3

Secondary structure

.................... 715
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: DAB07744B04CFEE2

FASTA71580,621
        10         20         30         40         50         60 
MSQSGAVSCC PGATNGSLGR SDGVAKMSPK DLFEQRKKYS NSNVIMHETS QYHVQHLATF 

        70         80         90        100        110        120 
IMDKSEAITS VDDAIRKLVQ LSSKEKIWTQ EMLLQVNDQS LRLLDIESQE ELEDFPLPTV 

       130        140        150        160        170        180 
QRSQTVLNQL RYPSVLLLVC QDSEQSKPDV HFFHCDEVEA ELVHEDIESA LADCRLGKKM 

       190        200        210        220        230        240 
RPQTLKGHQE KIRQRQSILP PPQGPAPIPF QHRGGDSPEA KNRVGPQVPL SEPGFRRRES 

       250        260        270        280        290        300 
QEEPRAVLAQ KIEKETQILN CALDDIEWFV ARLQKAAEAF KQLNQRKKGK KKGKKAPAEG 

       310        320        330        340        350        360 
VLTLRARPPS EGEFIDCFQK IKLAINLLAK LQKHIQNPSA AELVHFLFGP LDLIVNTCSG 

       370        380        390        400        410        420 
PDIARSVSCP LLSRDAVDFL RGHLVPKEMS LWESLGESWM RPRSEWPREP QVPLYVPKFH 

       430        440        450        460        470        480 
SGWEPPVDVL QEAPWEVEGL ASAPIEEVSP VSRQSIRNSQ KHSPTSEPTP PGDALPPVSS 

       490        500        510        520        530        540 
PHTHRGYQPT PAMAKYVKIL YDFTARNANE LSVLKDEVLE VLEDGRQWWK LRSRSGQAGY 

       550        560        570        580        590        600 
VPCNILGEAR PEDAGAPFEQ AGQKYWGPAS PTHKLPPSFP GNKDELMQHM DEVNDELIRK 

       610        620        630        640        650        660 
ISNIRAQPQR HFRVERSQPV SQPLTYESGP DEVRAWLEAK AFSPRIVENL GILTGPQLFS 

       670        680        690        700        710 
LNKEELKKVC GEEGVRVYSQ LTMQKAFLEK QQSGSELEEL MNKFHSMNQR RGEDS 

« Hide

Isoform 2 [UniParc].

Checksum: 9D933D44E34CC320
Show »

FASTA32737,113
Isoform 3 [UniParc].

Checksum: 9D355BAFCF33601B
Show »

FASTA73182,323

References

« Hide 'large scale' references
[1]"In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure."
Tocchetti A., Confalonieri S., Scita G., Di Fiore P.P., Betsholtz C.
Genomics 81:234-244(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Large-scale cDNA transfection screening for genes related to cancer development and progression."
Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. expand/collapse author list , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain cortex and Placenta.
[4]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Kidney.
[5]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Brain and Prostate.
[7]"The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH ABI1 AND SOS1, INTERACTION WITH ABI1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-449, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-469 AND SER-570, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"solution structure of the SH3 domain from human epidermal growth factor receptor pathway substrate 8-like protein."
RIKEN structural genomics initiative (RSGI)
Submitted (JUL-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 495-549.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY074929 mRNA. Translation: AAL76118.1.
AF318331 mRNA. Translation: AAL55838.1. Frameshift.
AK025588 mRNA. Translation: BAB15180.1. Different initiation.
AK025824 mRNA. Translation: BAB15248.1.
AK027765 mRNA. Translation: BAB55354.1.
AK094539 mRNA. Translation: BAG52883.1.
AK222903 mRNA. Translation: BAD96623.1.
BC080636 mRNA. Translation: AAH80636.1.
BC093878 mRNA. Translation: AAH93878.1.
BC101481 mRNA. Translation: AAI01482.1.
BC143242 mRNA. Translation: AAI43243.1.
AC131934 Genomic DNA. No translation available.
AP006621 Genomic DNA. No translation available.
RefSeqNP_073609.2. NM_022772.3.
UniGeneHs.55016.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WWUNMR-A612-697[»]
1WXBNMR-A495-549[»]
ProteinModelPortalQ9H6S3.
SMRQ9H6S3. Positions 46-175, 494-555, 612-697.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122297. 6 interactions.
IntActQ9H6S3. 5 interactions.
MINTMINT-5002000.
STRING9606.ENSP00000320828.

PTM databases

PhosphoSiteQ9H6S3.

Polymorphism databases

DMDM108864726.

Proteomic databases

PaxDbQ9H6S3.
PRIDEQ9H6S3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000318562; ENSP00000320828; ENSG00000177106. [Q9H6S3-1]
ENST00000526198; ENSP00000436230; ENSG00000177106.
ENST00000530636; ENSP00000436035; ENSG00000177106. [Q9H6S3-1]
ENST00000533256; ENSP00000435585; ENSG00000177106. [Q9H6S3-1]
GeneID64787.
KEGGhsa:64787.
UCSCuc001lqt.3. human. [Q9H6S3-1]
uc001lqw.3. human. [Q9H6S3-2]

Organism-specific databases

CTD64787.
GeneCardsGC11P000696.
HGNCHGNC:21296. EPS8L2.
HPAHPA041143.
MIM614988. gene.
neXtProtNX_Q9H6S3.
PharmGKBPA134981048.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG263108.
HOVERGENHBG003090.
InParanoidQ9H6S3.
KOK17277.
OrthoDBEOG7TMZR9.
PhylomeDBQ9H6S3.
TreeFamTF313069.

Gene expression databases

ArrayExpressQ9H6S3.
BgeeQ9H6S3.
CleanExHS_EPS8L2.
GenevestigatorQ9H6S3.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEPS8L2. human.
EvolutionaryTraceQ9H6S3.
GeneWikiEPS8L2.
GenomeRNAi64787.
NextBio66840.
PROQ9H6S3.
SOURCESearch...

Entry information

Entry nameES8L2_HUMAN
AccessionPrimary (citable) accession number: Q9H6S3
Secondary accession number(s): B3KSX1 expand/collapse secondary AC list , B7ZKL3, Q53GM8, Q8WYW7, Q96K06, Q9H6K9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM