Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5-azacytidine-induced protein 2

Gene

AZI2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.3 Publications

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

SignaLinkiQ9H6S1.

Names & Taxonomyi

Protein namesi
Recommended name:
5-azacytidine-induced protein 2
Alternative name(s):
NF-kappa-B-activating kinase-associated protein 1
Short name:
Nak-associated protein 1
TILP
Gene namesi
Name:AZI2
Synonyms:NAP1, TBKBP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:24002. AZI2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134950985.

Polymorphism and mutation databases

BioMutaiAZI2.
DMDMi74718550.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3923925-azacytidine-induced protein 2PRO_0000280602Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei318 – 3181PhosphoserineBy similarity
Modified residuei353 – 3531PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H6S1.
MaxQBiQ9H6S1.
PaxDbiQ9H6S1.
PeptideAtlasiQ9H6S1.
PRIDEiQ9H6S1.

PTM databases

iPTMnetiQ9H6S1.
PhosphoSiteiQ9H6S1.

Expressioni

Tissue specificityi

Widely expressed. Abundant expression seen in the pancreas and testis.1 Publication

Gene expression databases

BgeeiQ9H6S1.
CleanExiHS_AZI2.
ExpressionAtlasiQ9H6S1. baseline and differential.
GenevisibleiQ9H6S1. HS.

Organism-specific databases

HPAiHPA035258.
HPA040816.

Interactioni

Subunit structurei

Homodimer. Interacts with IKBKE, TBK1 and TICAM1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Q9E7P02EBI-359973,EBI-11361108From a different organism.
TBK1Q9UHD23EBI-359973,EBI-356402
VACWR022P173622EBI-359973,EBI-9519257From a different organism.

Protein-protein interaction databases

BioGridi122142. 15 interactions.
DIPiDIP-27605N.
IntActiQ9H6S1. 11 interactions.
MINTiMINT-1149019.
STRINGi9606.ENSP00000419371.

Structurei

3D structure databases

ProteinModelPortaliQ9H6S1.
SMRiQ9H6S1. Positions 41-73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 197197HomodimerizationBy similarityAdd
BLAST
Regioni216 – 25742Interaction with TBK1 and IKBKEAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili40 – 7637Sequence analysisAdd
BLAST
Coiled coili102 – 19695Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG4Z. Eukaryota.
ENOG4111IT0. LUCA.
GeneTreeiENSGT00390000001279.
HOGENOMiHOG000013059.
HOVERGENiHBG067551.
InParanoidiQ9H6S1.
KOiK12651.
OMAiTATYKRH.
PhylomeDBiQ9H6S1.
TreeFamiTF331289.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H6S1-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDALVEDDIC ILNHEKAHKR DTVTPVSIYS GDESVASHFA LVTAYEDIKK
60 70 80 90 100
RLKDSEKENS LLKKRIRFLE EKLIARFEEE TSSVGREQVN KAYHAYREVC
110 120 130 140 150
IDRDNLKSKL DKMNKDNSES LKVLNEQLQS KEVELLQLRT EVETQQVMRN
160 170 180 190 200
LNPPSSNWEV EKLSCDLKIH GLEQELELMR KECSDLKIEL QKAKQTDPYQ
210 220 230 240 250
EDNLKSRDLQ KLSISSDNMQ HAYWELKREM SNLHLVTQVQ AELLRKLKTS
260 270 280 290 300
TAIKKACAPV GCSEDLGRDS TKLHLMNFTA TYTRHPPLLP NGKALCHTTS
310 320 330 340 350
SPLPGDVKVL SEKAILQSWT DNERSIPNDG TCFQEHSSYG RNSLEDNSWV
360 370 380 390
FPSPPKSSET AFGETKTKTL PLPNLPPLHY LDQHNQNCLY KN
Length:392
Mass (Da):44,935
Last modified:March 1, 2001 - v1
Checksum:iEFDBDDA60C5CD624
GO
Isoform 2 (identifier: Q9H6S1-3) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     216-228: SDNMQHAYWELKR → TQPGVQWRNHGIA
     229-392: Missing.

Show »
Length:228
Mass (Da):26,510
Checksum:iA72E09E4DDF71599
GO
Isoform 3 (identifier: Q9H6S1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     216-243: SDNMQHAYWELKREMSNLHLVTQVQAEL → RQSICSTAWSAVAQSWDSMNFEDCSLFA
     244-392: Missing.

Note: Gene prediction based on EST data.
Show »
Length:243
Mass (Da):28,188
Checksum:i7AA3CF2551827226
GO
Isoform 4 (identifier: Q9H6S1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     216-232: SDNMQHAYWELKREMSN → RVSVAQPGVQWRNHGIA
     233-392: Missing.

Note: Gene prediction based on EST data.
Show »
Length:232
Mass (Da):26,921
Checksum:i5F5E73150C34543A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei216 – 24328SDNMQ…VQAEL → RQSICSTAWSAVAQSWDSMN FEDCSLFA in isoform 3. CuratedVSP_047087Add
BLAST
Alternative sequencei216 – 23217SDNMQ…REMSN → RVSVAQPGVQWRNHGIA in isoform 4. CuratedVSP_047088Add
BLAST
Alternative sequencei216 – 22813SDNMQ…WELKR → TQPGVQWRNHGIA in isoform 2. CuratedVSP_023817Add
BLAST
Alternative sequencei229 – 392164Missing in isoform 2. CuratedVSP_023818Add
BLAST
Alternative sequencei233 – 392160Missing in isoform 4. CuratedVSP_047089Add
BLAST
Alternative sequencei244 – 392149Missing in isoform 3. CuratedVSP_047090Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY151386 mRNA. Translation: AAO05967.1.
AF044917 Genomic DNA. Translation: AAF22368.1.
AF044918 Genomic DNA. Translation: AAF22369.1.
AK025592 mRNA. Translation: BAB15182.1.
AK290637 mRNA. Translation: BAF83326.1.
AC092503 Genomic DNA. No translation available.
AC098616 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64395.1.
CCDSiCCDS2647.1. [Q9H6S1-1]
CCDS46782.1. [Q9H6S1-4]
CCDS46783.1. [Q9H6S1-5]
RefSeqiNP_001127904.1. NM_001134432.1. [Q9H6S1-4]
NP_001127905.1. NM_001134433.1. [Q9H6S1-5]
NP_071906.1. NM_022461.4. [Q9H6S1-1]
XP_005265449.1. XM_005265392.1. [Q9H6S1-1]
UniGeneiHs.706676.

Genome annotation databases

EnsembliENST00000334100; ENSP00000335609; ENSG00000163512. [Q9H6S1-5]
ENST00000420543; ENSP00000391696; ENSG00000163512. [Q9H6S1-4]
ENST00000457172; ENSP00000389577; ENSG00000163512. [Q9H6S1-4]
ENST00000479665; ENSP00000419371; ENSG00000163512. [Q9H6S1-1]
GeneIDi64343.
KEGGihsa:64343.
UCSCiuc003ceb.5. human. [Q9H6S1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY151386 mRNA. Translation: AAO05967.1.
AF044917 Genomic DNA. Translation: AAF22368.1.
AF044918 Genomic DNA. Translation: AAF22369.1.
AK025592 mRNA. Translation: BAB15182.1.
AK290637 mRNA. Translation: BAF83326.1.
AC092503 Genomic DNA. No translation available.
AC098616 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64395.1.
CCDSiCCDS2647.1. [Q9H6S1-1]
CCDS46782.1. [Q9H6S1-4]
CCDS46783.1. [Q9H6S1-5]
RefSeqiNP_001127904.1. NM_001134432.1. [Q9H6S1-4]
NP_001127905.1. NM_001134433.1. [Q9H6S1-5]
NP_071906.1. NM_022461.4. [Q9H6S1-1]
XP_005265449.1. XM_005265392.1. [Q9H6S1-1]
UniGeneiHs.706676.

3D structure databases

ProteinModelPortaliQ9H6S1.
SMRiQ9H6S1. Positions 41-73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122142. 15 interactions.
DIPiDIP-27605N.
IntActiQ9H6S1. 11 interactions.
MINTiMINT-1149019.
STRINGi9606.ENSP00000419371.

PTM databases

iPTMnetiQ9H6S1.
PhosphoSiteiQ9H6S1.

Polymorphism and mutation databases

BioMutaiAZI2.
DMDMi74718550.

Proteomic databases

EPDiQ9H6S1.
MaxQBiQ9H6S1.
PaxDbiQ9H6S1.
PeptideAtlasiQ9H6S1.
PRIDEiQ9H6S1.

Protocols and materials databases

DNASUi64343.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334100; ENSP00000335609; ENSG00000163512. [Q9H6S1-5]
ENST00000420543; ENSP00000391696; ENSG00000163512. [Q9H6S1-4]
ENST00000457172; ENSP00000389577; ENSG00000163512. [Q9H6S1-4]
ENST00000479665; ENSP00000419371; ENSG00000163512. [Q9H6S1-1]
GeneIDi64343.
KEGGihsa:64343.
UCSCiuc003ceb.5. human. [Q9H6S1-1]

Organism-specific databases

CTDi64343.
GeneCardsiAZI2.
HGNCiHGNC:24002. AZI2.
HPAiHPA035258.
HPA040816.
MIMi609916. gene.
neXtProtiNX_Q9H6S1.
PharmGKBiPA134950985.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG4Z. Eukaryota.
ENOG4111IT0. LUCA.
GeneTreeiENSGT00390000001279.
HOGENOMiHOG000013059.
HOVERGENiHBG067551.
InParanoidiQ9H6S1.
KOiK12651.
OMAiTATYKRH.
PhylomeDBiQ9H6S1.
TreeFamiTF331289.

Enzyme and pathway databases

SignaLinkiQ9H6S1.

Miscellaneous databases

ChiTaRSiAZI2. human.
GeneWikiiAZI2.
GenomeRNAii64343.
PROiQ9H6S1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H6S1.
CleanExiHS_AZI2.
ExpressionAtlasiQ9H6S1. baseline and differential.
GenevisibleiQ9H6S1. HS.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of NAP1, a regulatory subunit of IkappaB kinase-related kinases that potentiates NF-kappaB signaling."
    Fujita F., Taniguchi Y., Kato T., Narita Y., Furuya A., Ogawa T., Sakurai H., Joh T., Itoh M., Delhase M., Karin M., Nakanishi M.
    Mol. Cell. Biol. 23:7780-7793(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH TBK1 AND IKBKE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. Inohara N., Koseki T., Nunez G.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 2).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryo and Hepatoma.
  4. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "NF-kappaB-activating kinase-associated protein 1 participates in TLR3/Toll-IL-1 homology domain-containing adapter molecule-1-mediated IFN regulatory factor 3 activation."
    Sasai M., Oshiumi H., Matsumoto M., Inoue N., Fujita F., Nakanishi M., Seya T.
    J. Immunol. 174:27-30(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TICAM1.
  7. "SINTBAD, a novel component of innate antiviral immunity, shares a TBK1-binding domain with NAP1 and TANK."
    Ryzhakov G., Randow F.
    EMBO J. 26:3180-3190(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IKBKE.
  8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: FUNCTION, INTERACTION WITH TBK1.
  11. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Erythroleukemia.
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiAZI2_HUMAN
AccessioniPrimary (citable) accession number: Q9H6S1
Secondary accession number(s): A8K3M2
, C9JB40, H7BXU6, Q86W99, Q9BQF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.