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Protein

5-azacytidine-induced protein 2

Gene

AZI2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.3 Publications

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163512-MONOMER.
SignaLinkiQ9H6S1.

Names & Taxonomyi

Protein namesi
Recommended name:
5-azacytidine-induced protein 2
Alternative name(s):
NF-kappa-B-activating kinase-associated protein 1
Short name:
Nak-associated protein 1
TILP
Gene namesi
Name:AZI2
Synonyms:NAP1, TBKBP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:24002. AZI2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi64343.
OpenTargetsiENSG00000163512.
PharmGKBiPA134950985.

Polymorphism and mutation databases

BioMutaiAZI2.
DMDMi74718550.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002806021 – 3925-azacytidine-induced protein 2Add BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei318PhosphoserineBy similarity1
Modified residuei353PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H6S1.
MaxQBiQ9H6S1.
PaxDbiQ9H6S1.
PeptideAtlasiQ9H6S1.
PRIDEiQ9H6S1.

PTM databases

iPTMnetiQ9H6S1.
PhosphoSitePlusiQ9H6S1.

Expressioni

Tissue specificityi

Widely expressed. Abundant expression seen in the pancreas and testis.1 Publication

Gene expression databases

BgeeiENSG00000163512.
CleanExiHS_AZI2.
ExpressionAtlasiQ9H6S1. baseline and differential.
GenevisibleiQ9H6S1. HS.

Organism-specific databases

HPAiHPA035258.
HPA040816.

Interactioni

Subunit structurei

Homodimer. Interacts with IKBKE, TBK1 and TICAM1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Q9E7P02EBI-359973,EBI-11361108From a different organism.
TBK1Q9UHD23EBI-359973,EBI-356402
VACWR022P173622EBI-359973,EBI-9519257From a different organism.

Protein-protein interaction databases

BioGridi122142. 15 interactors.
DIPiDIP-27605N.
IntActiQ9H6S1. 12 interactors.
MINTiMINT-1149019.
STRINGi9606.ENSP00000419371.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EP6X-ray1.45A/C215-255[»]
ProteinModelPortaliQ9H6S1.
SMRiQ9H6S1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 197HomodimerizationBy similarityAdd BLAST197
Regioni216 – 257Interaction with TBK1 and IKBKEAdd BLAST42

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili40 – 76Sequence analysisAdd BLAST37
Coiled coili102 – 196Sequence analysisAdd BLAST95

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG4Z. Eukaryota.
ENOG4111IT0. LUCA.
GeneTreeiENSGT00390000001279.
HOGENOMiHOG000013059.
HOVERGENiHBG067551.
InParanoidiQ9H6S1.
KOiK12651.
OMAiKDSTKLH.
OrthoDBiEOG091G09UX.
PhylomeDBiQ9H6S1.
TreeFamiTF331289.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H6S1-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDALVEDDIC ILNHEKAHKR DTVTPVSIYS GDESVASHFA LVTAYEDIKK
60 70 80 90 100
RLKDSEKENS LLKKRIRFLE EKLIARFEEE TSSVGREQVN KAYHAYREVC
110 120 130 140 150
IDRDNLKSKL DKMNKDNSES LKVLNEQLQS KEVELLQLRT EVETQQVMRN
160 170 180 190 200
LNPPSSNWEV EKLSCDLKIH GLEQELELMR KECSDLKIEL QKAKQTDPYQ
210 220 230 240 250
EDNLKSRDLQ KLSISSDNMQ HAYWELKREM SNLHLVTQVQ AELLRKLKTS
260 270 280 290 300
TAIKKACAPV GCSEDLGRDS TKLHLMNFTA TYTRHPPLLP NGKALCHTTS
310 320 330 340 350
SPLPGDVKVL SEKAILQSWT DNERSIPNDG TCFQEHSSYG RNSLEDNSWV
360 370 380 390
FPSPPKSSET AFGETKTKTL PLPNLPPLHY LDQHNQNCLY KN
Length:392
Mass (Da):44,935
Last modified:March 1, 2001 - v1
Checksum:iEFDBDDA60C5CD624
GO
Isoform 2 (identifier: Q9H6S1-3) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     216-228: SDNMQHAYWELKR → TQPGVQWRNHGIA
     229-392: Missing.

Show »
Length:228
Mass (Da):26,510
Checksum:iA72E09E4DDF71599
GO
Isoform 3 (identifier: Q9H6S1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     216-243: SDNMQHAYWELKREMSNLHLVTQVQAEL → RQSICSTAWSAVAQSWDSMNFEDCSLFA
     244-392: Missing.

Note: Gene prediction based on EST data.
Show »
Length:243
Mass (Da):28,188
Checksum:i7AA3CF2551827226
GO
Isoform 4 (identifier: Q9H6S1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     216-232: SDNMQHAYWELKREMSN → RVSVAQPGVQWRNHGIA
     233-392: Missing.

Note: Gene prediction based on EST data.
Show »
Length:232
Mass (Da):26,921
Checksum:i5F5E73150C34543A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047087216 – 243SDNMQ…VQAEL → RQSICSTAWSAVAQSWDSMN FEDCSLFA in isoform 3. CuratedAdd BLAST28
Alternative sequenceiVSP_047088216 – 232SDNMQ…REMSN → RVSVAQPGVQWRNHGIA in isoform 4. CuratedAdd BLAST17
Alternative sequenceiVSP_023817216 – 228SDNMQ…WELKR → TQPGVQWRNHGIA in isoform 2. CuratedAdd BLAST13
Alternative sequenceiVSP_023818229 – 392Missing in isoform 2. CuratedAdd BLAST164
Alternative sequenceiVSP_047089233 – 392Missing in isoform 4. CuratedAdd BLAST160
Alternative sequenceiVSP_047090244 – 392Missing in isoform 3. CuratedAdd BLAST149

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY151386 mRNA. Translation: AAO05967.1.
AF044917 Genomic DNA. Translation: AAF22368.1.
AF044918 Genomic DNA. Translation: AAF22369.1.
AK025592 mRNA. Translation: BAB15182.1.
AK290637 mRNA. Translation: BAF83326.1.
AC092503 Genomic DNA. No translation available.
AC098616 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64395.1.
CCDSiCCDS2647.1. [Q9H6S1-1]
CCDS46782.1. [Q9H6S1-4]
CCDS46783.1. [Q9H6S1-5]
RefSeqiNP_001127904.1. NM_001134432.1. [Q9H6S1-4]
NP_001127905.1. NM_001134433.1. [Q9H6S1-5]
NP_071906.1. NM_022461.4. [Q9H6S1-1]
XP_005265449.1. XM_005265392.2. [Q9H6S1-1]
UniGeneiHs.706676.

Genome annotation databases

EnsembliENST00000334100; ENSP00000335609; ENSG00000163512. [Q9H6S1-5]
ENST00000420543; ENSP00000391696; ENSG00000163512. [Q9H6S1-4]
ENST00000457172; ENSP00000389577; ENSG00000163512. [Q9H6S1-4]
ENST00000479665; ENSP00000419371; ENSG00000163512. [Q9H6S1-1]
GeneIDi64343.
KEGGihsa:64343.
UCSCiuc003ceb.5. human. [Q9H6S1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY151386 mRNA. Translation: AAO05967.1.
AF044917 Genomic DNA. Translation: AAF22368.1.
AF044918 Genomic DNA. Translation: AAF22369.1.
AK025592 mRNA. Translation: BAB15182.1.
AK290637 mRNA. Translation: BAF83326.1.
AC092503 Genomic DNA. No translation available.
AC098616 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64395.1.
CCDSiCCDS2647.1. [Q9H6S1-1]
CCDS46782.1. [Q9H6S1-4]
CCDS46783.1. [Q9H6S1-5]
RefSeqiNP_001127904.1. NM_001134432.1. [Q9H6S1-4]
NP_001127905.1. NM_001134433.1. [Q9H6S1-5]
NP_071906.1. NM_022461.4. [Q9H6S1-1]
XP_005265449.1. XM_005265392.2. [Q9H6S1-1]
UniGeneiHs.706676.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EP6X-ray1.45A/C215-255[»]
ProteinModelPortaliQ9H6S1.
SMRiQ9H6S1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122142. 15 interactors.
DIPiDIP-27605N.
IntActiQ9H6S1. 12 interactors.
MINTiMINT-1149019.
STRINGi9606.ENSP00000419371.

PTM databases

iPTMnetiQ9H6S1.
PhosphoSitePlusiQ9H6S1.

Polymorphism and mutation databases

BioMutaiAZI2.
DMDMi74718550.

Proteomic databases

EPDiQ9H6S1.
MaxQBiQ9H6S1.
PaxDbiQ9H6S1.
PeptideAtlasiQ9H6S1.
PRIDEiQ9H6S1.

Protocols and materials databases

DNASUi64343.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334100; ENSP00000335609; ENSG00000163512. [Q9H6S1-5]
ENST00000420543; ENSP00000391696; ENSG00000163512. [Q9H6S1-4]
ENST00000457172; ENSP00000389577; ENSG00000163512. [Q9H6S1-4]
ENST00000479665; ENSP00000419371; ENSG00000163512. [Q9H6S1-1]
GeneIDi64343.
KEGGihsa:64343.
UCSCiuc003ceb.5. human. [Q9H6S1-1]

Organism-specific databases

CTDi64343.
DisGeNETi64343.
GeneCardsiAZI2.
HGNCiHGNC:24002. AZI2.
HPAiHPA035258.
HPA040816.
MIMi609916. gene.
neXtProtiNX_Q9H6S1.
OpenTargetsiENSG00000163512.
PharmGKBiPA134950985.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG4Z. Eukaryota.
ENOG4111IT0. LUCA.
GeneTreeiENSGT00390000001279.
HOGENOMiHOG000013059.
HOVERGENiHBG067551.
InParanoidiQ9H6S1.
KOiK12651.
OMAiKDSTKLH.
OrthoDBiEOG091G09UX.
PhylomeDBiQ9H6S1.
TreeFamiTF331289.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163512-MONOMER.
SignaLinkiQ9H6S1.

Miscellaneous databases

ChiTaRSiAZI2. human.
GeneWikiiAZI2.
GenomeRNAii64343.
PROiQ9H6S1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163512.
CleanExiHS_AZI2.
ExpressionAtlasiQ9H6S1. baseline and differential.
GenevisibleiQ9H6S1. HS.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAZI2_HUMAN
AccessioniPrimary (citable) accession number: Q9H6S1
Secondary accession number(s): A8K3M2
, C9JB40, H7BXU6, Q86W99, Q9BQF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.