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Protein

Putative ATP-dependent RNA helicase DHX33

Gene

DHX33

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. Important element of nucleolar organization.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi97 – 104ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB
  • rDNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000005100-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase DHX33 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 33
Gene namesi
Name:DHX33
Synonyms:DDX33
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:16718. DHX33.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000005100.
PharmGKBiPA27220.

Polymorphism and mutation databases

BioMutaiDHX33.
DMDMi296434478.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551641 – 707Putative ATP-dependent RNA helicase DHX33Add BLAST707

Proteomic databases

EPDiQ9H6R0.
MaxQBiQ9H6R0.
PaxDbiQ9H6R0.
PeptideAtlasiQ9H6R0.
PRIDEiQ9H6R0.

PTM databases

iPTMnetiQ9H6R0.
PhosphoSitePlusiQ9H6R0.

Expressioni

Gene expression databases

BgeeiENSG00000005100.
CleanExiHS_DHX33.
ExpressionAtlasiQ9H6R0. baseline and differential.
GenevisibleiQ9H6R0. HS.

Organism-specific databases

HPAiHPA071536.

Interactioni

Subunit structurei

Interacts with UBTF.1 Publication

Protein-protein interaction databases

BioGridi121247. 16 interactors.
IntActiQ9H6R0. 10 interactors.
MINTiMINT-3068519.
STRINGi9606.ENSP00000225296.

Structurei

3D structure databases

ProteinModelPortaliQ9H6R0.
SMRiQ9H6R0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 252Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini277 – 450Helicase C-terminalPROSITE-ProRule annotationAdd BLAST174

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi194 – 197DEAH box4
Motifi547 – 558Critical for rDNA-bindingBy similarityAdd BLAST12

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0922. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ9H6R0.
KOiK17820.
OMAiPMGRKMA.
OrthoDBiEOG091G030Q.
PhylomeDBiQ9H6R0.
TreeFamiTF354245.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H6R0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPEEAGFPPA KRFRPGSGPP SRAGSFPPGR QVVMLLTAGS GGRGGGGGRR
60 70 80 90 100
QQPPLAQPSA SPYPEAVELQ RRSLPIFQAR GQLLAQLRNL DNAVLIGETG
110 120 130 140 150
SGKTTQIPQY LYEGGISRQG IIAVTQPRRV AAISLATRVS DEKRTELGKL
160 170 180 190 200
VGYTVRFDDV TSEDTRIKFL TDGMLLREAI SDSLLRKYSC VILDEAHERT
210 220 230 240 250
IHTDVLFGVV KAAQKRRKEL GKLPLKVIVM SATMDVDLFS QYFNGAPVLY
260 270 280 290 300
LEGRQHPIQV FYTKQPQNDY LHAALVSVFQ IHQEAPSSQD ILVFLTGQEE
310 320 330 340 350
IEAMSKTCRD IAKHLPDGCP AMLVLPLYAS LPYAQQLRVF QGAPKGYRKV
360 370 380 390 400
IISTNIAETS ITITGIKYVV DTGMVKAKKY NPDSGLEVLA VQRVSKTQAW
410 420 430 440 450
QRTGRAGRED SGICYRLYTE DEFEKFDKMT VPEIQRCNLA SVMLQLLAMK
460 470 480 490 500
VPNVLTFDFM SKPSPDHIQA AIAQLDLLGA LEHKDDQLTL TPMGRKMAAF
510 520 530 540 550
PLEPKFAKTI LMSPKFHCTE EILTIVSLLS VDSVLHNPPS RREEVQGVRK
560 570 580 590 600
KFISSEGDHM TLLNIYRTFK NLGGNKDWCK ENFVNSKNMT LVAEVRAQLR
610 620 630 640 650
DICLKMSMPI ASSRGDVESV RRCLAHSLFM STAELQPDGT YATTDTHQPV
660 670 680 690 700
AIHPSSVLFH CKPACVVYTE LLYTNKCYMR DLCVIDAQWL YEAAPEYFRR

KLRTARN
Length:707
Mass (Da):78,874
Last modified:May 18, 2010 - v2
Checksum:iE3515B12BD972431
GO
Isoform 2 (identifier: Q9H6R0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-173: Missing.

Show »
Length:534
Mass (Da):60,393
Checksum:iA82774C3D3FC205E
GO

Sequence cautioni

The sequence AAH30017 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77F → L in BAB15193 (PubMed:14702039).Curated1
Sequence conflicti166R → G in BAB15193 (PubMed:14702039).Curated1
Sequence conflicti377A → V in AAH30017 (PubMed:15489334).Curated1
Sequence conflicti539P → S in CAI56793 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057239118R → C.Corresponds to variant rs8069315dbSNPEnsembl.1
Natural variantiVAR_057240483H → D.Corresponds to variant rs11653658dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0162561 – 173Missing in isoform 2. 2 PublicationsAdd BLAST173

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK025625 mRNA. Translation: BAB15193.1.
AK026944 mRNA. Translation: BAB15596.1.
AK295074 mRNA. Translation: BAG58120.1.
AC004148 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90332.1.
BC030017 mRNA. Translation: AAH30017.1. Different initiation.
CR936655 mRNA. Translation: CAI56793.1.
CCDSiCCDS11072.1. [Q9H6R0-1]
RefSeqiNP_001186628.1. NM_001199699.1. [Q9H6R0-2]
NP_064547.2. NM_020162.3. [Q9H6R0-1]
XP_016880366.1. XM_017024877.1.
UniGeneiHs.250456.

Genome annotation databases

EnsembliENST00000225296; ENSP00000225296; ENSG00000005100. [Q9H6R0-1]
GeneIDi56919.
KEGGihsa:56919.
UCSCiuc002gca.3. human. [Q9H6R0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK025625 mRNA. Translation: BAB15193.1.
AK026944 mRNA. Translation: BAB15596.1.
AK295074 mRNA. Translation: BAG58120.1.
AC004148 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90332.1.
BC030017 mRNA. Translation: AAH30017.1. Different initiation.
CR936655 mRNA. Translation: CAI56793.1.
CCDSiCCDS11072.1. [Q9H6R0-1]
RefSeqiNP_001186628.1. NM_001199699.1. [Q9H6R0-2]
NP_064547.2. NM_020162.3. [Q9H6R0-1]
XP_016880366.1. XM_017024877.1.
UniGeneiHs.250456.

3D structure databases

ProteinModelPortaliQ9H6R0.
SMRiQ9H6R0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121247. 16 interactors.
IntActiQ9H6R0. 10 interactors.
MINTiMINT-3068519.
STRINGi9606.ENSP00000225296.

PTM databases

iPTMnetiQ9H6R0.
PhosphoSitePlusiQ9H6R0.

Polymorphism and mutation databases

BioMutaiDHX33.
DMDMi296434478.

Proteomic databases

EPDiQ9H6R0.
MaxQBiQ9H6R0.
PaxDbiQ9H6R0.
PeptideAtlasiQ9H6R0.
PRIDEiQ9H6R0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225296; ENSP00000225296; ENSG00000005100. [Q9H6R0-1]
GeneIDi56919.
KEGGihsa:56919.
UCSCiuc002gca.3. human. [Q9H6R0-1]

Organism-specific databases

CTDi56919.
GeneCardsiDHX33.
HGNCiHGNC:16718. DHX33.
HPAiHPA071536.
MIMi614405. gene.
neXtProtiNX_Q9H6R0.
OpenTargetsiENSG00000005100.
PharmGKBiPA27220.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0922. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ9H6R0.
KOiK17820.
OMAiPMGRKMA.
OrthoDBiEOG091G030Q.
PhylomeDBiQ9H6R0.
TreeFamiTF354245.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000005100-MONOMER.

Miscellaneous databases

ChiTaRSiDHX33. human.
GenomeRNAii56919.
PROiQ9H6R0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000005100.
CleanExiHS_DHX33.
ExpressionAtlasiQ9H6R0. baseline and differential.
GenevisibleiQ9H6R0. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHX33_HUMAN
AccessioniPrimary (citable) accession number: Q9H6R0
Secondary accession number(s): B4DHF9
, Q4G149, Q5CZ73, Q9H5M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 18, 2010
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.