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Q9H6R0

- DHX33_HUMAN

UniProt

Q9H6R0 - DHX33_HUMAN

Protein

Putative ATP-dependent RNA helicase DHX33

Gene

DHX33

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (18 May 2010)
      Previous versions | rss
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    Functioni

    Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. Important element of nucleolar organization.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi97 – 1048ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. poly(A) RNA binding Source: UniProtKB
    4. rDNA binding Source: UniProtKB

    GO - Biological processi

    1. positive regulation of transcription from RNA polymerase I promoter Source: UniProtKB

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative ATP-dependent RNA helicase DHX33 (EC:3.6.4.13)
    Alternative name(s):
    DEAH box protein 33
    Gene namesi
    Name:DHX33
    Synonyms:DDX33
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:16718. DHX33.

    Subcellular locationi

    Nucleusnucleolus. Nucleusnucleoplasm
    Note: Predominantly in the nucleolus. During mitosis, localizes with the nucleolar organizing regions.

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB
    2. nucleoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27220.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 707707Putative ATP-dependent RNA helicase DHX33PRO_0000055164Add
    BLAST

    Proteomic databases

    MaxQBiQ9H6R0.
    PaxDbiQ9H6R0.
    PRIDEiQ9H6R0.

    PTM databases

    PhosphoSiteiQ9H6R0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9H6R0.
    BgeeiQ9H6R0.
    CleanExiHS_DHX33.
    GenevestigatoriQ9H6R0.

    Interactioni

    Subunit structurei

    Interacts with UBTF.1 Publication

    Protein-protein interaction databases

    BioGridi121247. 7 interactions.
    IntActiQ9H6R0. 3 interactions.
    MINTiMINT-3068519.
    STRINGi9606.ENSP00000225296.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H6R0.
    SMRiQ9H6R0. Positions 71-698.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini84 – 252169Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini277 – 450174Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi194 – 1974DEAH box
    Motifi547 – 55812Critical for rDNA-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1643.
    HOGENOMiHOG000175261.
    HOVERGENiHBG039428.
    InParanoidiQ9H6R0.
    KOiK17820.
    OMAiGDMESVR.
    OrthoDBiEOG718KC0.
    PhylomeDBiQ9H6R0.
    TreeFamiTF354245.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9H6R0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPEEAGFPPA KRFRPGSGPP SRAGSFPPGR QVVMLLTAGS GGRGGGGGRR    50
    QQPPLAQPSA SPYPEAVELQ RRSLPIFQAR GQLLAQLRNL DNAVLIGETG 100
    SGKTTQIPQY LYEGGISRQG IIAVTQPRRV AAISLATRVS DEKRTELGKL 150
    VGYTVRFDDV TSEDTRIKFL TDGMLLREAI SDSLLRKYSC VILDEAHERT 200
    IHTDVLFGVV KAAQKRRKEL GKLPLKVIVM SATMDVDLFS QYFNGAPVLY 250
    LEGRQHPIQV FYTKQPQNDY LHAALVSVFQ IHQEAPSSQD ILVFLTGQEE 300
    IEAMSKTCRD IAKHLPDGCP AMLVLPLYAS LPYAQQLRVF QGAPKGYRKV 350
    IISTNIAETS ITITGIKYVV DTGMVKAKKY NPDSGLEVLA VQRVSKTQAW 400
    QRTGRAGRED SGICYRLYTE DEFEKFDKMT VPEIQRCNLA SVMLQLLAMK 450
    VPNVLTFDFM SKPSPDHIQA AIAQLDLLGA LEHKDDQLTL TPMGRKMAAF 500
    PLEPKFAKTI LMSPKFHCTE EILTIVSLLS VDSVLHNPPS RREEVQGVRK 550
    KFISSEGDHM TLLNIYRTFK NLGGNKDWCK ENFVNSKNMT LVAEVRAQLR 600
    DICLKMSMPI ASSRGDVESV RRCLAHSLFM STAELQPDGT YATTDTHQPV 650
    AIHPSSVLFH CKPACVVYTE LLYTNKCYMR DLCVIDAQWL YEAAPEYFRR 700
    KLRTARN 707
    Length:707
    Mass (Da):78,874
    Last modified:May 18, 2010 - v2
    Checksum:iE3515B12BD972431
    GO
    Isoform 2 (identifier: Q9H6R0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-173: Missing.

    Show »
    Length:534
    Mass (Da):60,393
    Checksum:iA82774C3D3FC205E
    GO

    Sequence cautioni

    The sequence AAH30017.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 771F → L in BAB15193. (PubMed:14702039)Curated
    Sequence conflicti166 – 1661R → G in BAB15193. (PubMed:14702039)Curated
    Sequence conflicti377 – 3771A → V in AAH30017. (PubMed:15489334)Curated
    Sequence conflicti539 – 5391P → S in CAI56793. (PubMed:17974005)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti118 – 1181R → C.
    Corresponds to variant rs8069315 [ dbSNP | Ensembl ].
    VAR_057239
    Natural varianti483 – 4831H → D.
    Corresponds to variant rs11653658 [ dbSNP | Ensembl ].
    VAR_057240

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 173173Missing in isoform 2. 2 PublicationsVSP_016256Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK025625 mRNA. Translation: BAB15193.1.
    AK026944 mRNA. Translation: BAB15596.1.
    AK295074 mRNA. Translation: BAG58120.1.
    AC004148 Genomic DNA. No translation available.
    CH471108 Genomic DNA. Translation: EAW90332.1.
    BC030017 mRNA. Translation: AAH30017.1. Different initiation.
    CR936655 mRNA. Translation: CAI56793.1.
    CCDSiCCDS11072.1. [Q9H6R0-1]
    RefSeqiNP_001186628.1. NM_001199699.1. [Q9H6R0-2]
    NP_064547.2. NM_020162.3. [Q9H6R0-1]
    UniGeneiHs.250456.

    Genome annotation databases

    EnsembliENST00000225296; ENSP00000225296; ENSG00000005100. [Q9H6R0-1]
    GeneIDi56919.
    KEGGihsa:56919.
    UCSCiuc002gbz.3. human. [Q9H6R0-1]

    Polymorphism databases

    DMDMi296434478.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK025625 mRNA. Translation: BAB15193.1 .
    AK026944 mRNA. Translation: BAB15596.1 .
    AK295074 mRNA. Translation: BAG58120.1 .
    AC004148 Genomic DNA. No translation available.
    CH471108 Genomic DNA. Translation: EAW90332.1 .
    BC030017 mRNA. Translation: AAH30017.1 . Different initiation.
    CR936655 mRNA. Translation: CAI56793.1 .
    CCDSi CCDS11072.1. [Q9H6R0-1 ]
    RefSeqi NP_001186628.1. NM_001199699.1. [Q9H6R0-2 ]
    NP_064547.2. NM_020162.3. [Q9H6R0-1 ]
    UniGenei Hs.250456.

    3D structure databases

    ProteinModelPortali Q9H6R0.
    SMRi Q9H6R0. Positions 71-698.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121247. 7 interactions.
    IntActi Q9H6R0. 3 interactions.
    MINTi MINT-3068519.
    STRINGi 9606.ENSP00000225296.

    PTM databases

    PhosphoSitei Q9H6R0.

    Polymorphism databases

    DMDMi 296434478.

    Proteomic databases

    MaxQBi Q9H6R0.
    PaxDbi Q9H6R0.
    PRIDEi Q9H6R0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000225296 ; ENSP00000225296 ; ENSG00000005100 . [Q9H6R0-1 ]
    GeneIDi 56919.
    KEGGi hsa:56919.
    UCSCi uc002gbz.3. human. [Q9H6R0-1 ]

    Organism-specific databases

    CTDi 56919.
    GeneCardsi GC17M005360.
    HGNCi HGNC:16718. DHX33.
    MIMi 614405. gene.
    neXtProti NX_Q9H6R0.
    PharmGKBi PA27220.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1643.
    HOGENOMi HOG000175261.
    HOVERGENi HBG039428.
    InParanoidi Q9H6R0.
    KOi K17820.
    OMAi GDMESVR.
    OrthoDBi EOG718KC0.
    PhylomeDBi Q9H6R0.
    TreeFami TF354245.

    Miscellaneous databases

    ChiTaRSi DHX33. human.
    GenomeRNAii 56919.
    NextBioi 62420.
    PROi Q9H6R0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9H6R0.
    Bgeei Q9H6R0.
    CleanExi HS_DHX33.
    Genevestigatori Q9H6R0.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain and Hepatoma.
    2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Testis.
    5. Bienvenut W.V.
      Submitted (AUG-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 119-128; 130-138 AND 200-211, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Cervix carcinoma.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 293-707.
      Tissue: Melanoma.
    7. "Identification of DHX33 as a mediator of rRNA synthesis and cell growth."
      Zhang Y., Forys J.T., Miceli A.P., Gwinn A.S., Weber J.D.
      Mol. Cell. Biol. 31:4676-4691(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH RIBOSOMAL DNA LOCI, INTERACTION WITH UBTF, SUBCELLULAR LOCATION.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDHX33_HUMAN
    AccessioniPrimary (citable) accession number: Q9H6R0
    Secondary accession number(s): B4DHF9
    , Q4G149, Q5CZ73, Q9H5M9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3