Q9H6R0 (DHX33_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative ATP-dependent RNA helicase DHX33
EC=3.6.4.13

Alternative name(s):
DEAH box protein 33

Gene names

Name:	DHX33
Synonyms:	DDX33

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Nucleus › nucleolus Ref.5.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence AAH30017.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H6R0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H6R0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-173: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	Putative ATP-dependent RNA helicase DHX33		PRO_0000055164
Regions
	Domain	84 – 252	169	Helicase ATP-binding
	Domain	277 – 450	174	Helicase C-terminal
	Nucleotide binding	97 – 104	8	ATP By similarity
	Motif	194 – 197	4	DEAH box
Amino acid modifications
	Modified residue	134	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 173	173	Missing in isoform 2.		VSP_016256
	Natural variant	118	1	R → C. Corresponds to variant rs8069315 [ dbSNP | Ensembl ].		VAR_057239
	Natural variant	483	1	H → D. Corresponds to variant rs11653658 [ dbSNP | Ensembl ].		VAR_057240
Experimental info
	Sequence conflict	77	1	F → L in BAB15193. Ref.1
	Sequence conflict	166	1	R → G in BAB15193. Ref.1
	Sequence conflict	377	1	A → V in AAH30017. Ref.4
	Sequence conflict	539	1	P → S in CAI56793. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: E3515B12BD972431	FASTA	707	78,874
	10 20 30 40 50 60 MPEEAGFPPA KRFRPGSGPP SRAGSFPPGR QVVMLLTAGS GGRGGGGGRR QQPPLAQPSA 70 80 90 100 110 120 SPYPEAVELQ RRSLPIFQAR GQLLAQLRNL DNAVLIGETG SGKTTQIPQY LYEGGISRQG 130 140 150 160 170 180 IIAVTQPRRV AAISLATRVS DEKRTELGKL VGYTVRFDDV TSEDTRIKFL TDGMLLREAI 190 200 210 220 230 240 SDSLLRKYSC VILDEAHERT IHTDVLFGVV KAAQKRRKEL GKLPLKVIVM SATMDVDLFS 250 260 270 280 290 300 QYFNGAPVLY LEGRQHPIQV FYTKQPQNDY LHAALVSVFQ IHQEAPSSQD ILVFLTGQEE 310 320 330 340 350 360 IEAMSKTCRD IAKHLPDGCP AMLVLPLYAS LPYAQQLRVF QGAPKGYRKV IISTNIAETS 370 380 390 400 410 420 ITITGIKYVV DTGMVKAKKY NPDSGLEVLA VQRVSKTQAW QRTGRAGRED SGICYRLYTE 430 440 450 460 470 480 DEFEKFDKMT VPEIQRCNLA SVMLQLLAMK VPNVLTFDFM SKPSPDHIQA AIAQLDLLGA 490 500 510 520 530 540 LEHKDDQLTL TPMGRKMAAF PLEPKFAKTI LMSPKFHCTE EILTIVSLLS VDSVLHNPPS 550 560 570 580 590 600 RREEVQGVRK KFISSEGDHM TLLNIYRTFK NLGGNKDWCK ENFVNSKNMT LVAEVRAQLR 610 620 630 640 650 660 DICLKMSMPI ASSRGDVESV RRCLAHSLFM STAELQPDGT YATTDTHQPV AIHPSSVLFH 670 680 690 700 CKPACVVYTE LLYTNKCYMR DLCVIDAQWL YEAAPEYFRR KLRTARN
	Isoform 2 [UniParc]. Checksum: A82774C3D3FC205E	FASTA	534	60,393
	10 20 30 40 50 60 MLLREAISDS LLRKYSCVIL DEAHERTIHT DVLFGVVKAA QKRRKELGKL PLKVIVMSAT 70 80 90 100 110 120 MDVDLFSQYF NGAPVLYLEG RQHPIQVFYT KQPQNDYLHA ALVSVFQIHQ EAPSSQDILV 130 140 150 160 170 180 FLTGQEEIEA MSKTCRDIAK HLPDGCPAML VLPLYASLPY AQQLRVFQGA PKGYRKVIIS 190 200 210 220 230 240 TNIAETSITI TGIKYVVDTG MVKAKKYNPD SGLEVLAVQR VSKTQAWQRT GRAGREDSGI 250 260 270 280 290 300 CYRLYTEDEF EKFDKMTVPE IQRCNLASVM LQLLAMKVPN VLTFDFMSKP SPDHIQAAIA 310 320 330 340 350 360 QLDLLGALEH KDDQLTLTPM GRKMAAFPLE PKFAKTILMS PKFHCTEEIL TIVSLLSVDS 370 380 390 400 410 420 VLHNPPSRRE EVQGVRKKFI SSEGDHMTLL NIYRTFKNLG GNKDWCKENF VNSKNMTLVA 430 440 450 460 470 480 EVRAQLRDIC LKMSMPIASS RGDVESVRRC LAHSLFMSTA ELQPDGTYAT TDTHQPVAIH 490 500 510 520 530 PSSVLFHCKP ACVVYTELLY TNKCYMRDLC VIDAQWLYEA APEYFRRKLR TARN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Hepatoma.
[2]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[5]	Bienvenut W.V. Submitted (AUG-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 119-128; 130-138 AND 200-211, SUBCELLULAR LOCATION, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 293-707. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK025625 mRNA. Translation: BAB15193.1. AK026944 mRNA. Translation: BAB15596.1. AK295074 mRNA. Translation: BAG58120.1. AC004148 Genomic DNA. No translation available. CH471108 Genomic DNA. Translation: EAW90332.1. BC030017 mRNA. Translation: AAH30017.1. Different initiation. CR936655 mRNA. Translation: CAI56793.1.
IPI	IPI00302860. IPI00655950.
RefSeq	NP_001186628.1. NM_001199699.1. NP_064547.2. NM_020162.3.
UniGene	Hs.250456.
3D structure databases
ProteinModelPortal	Q9H6R0.
SMR	Q9H6R0. Positions 49-701.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H6R0. 1 interaction.
PTM databases
PhosphoSite	Q9H6R0.
Polymorphism databases
DMDM	296434478.
Proteomic databases
PRIDE	Q9H6R0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000225296; ENSP00000225296; ENSG00000005100. ENST00000457531; ENSP00000389066; ENSG00000005100.
GeneID	56919.
KEGG	hsa:56919.
UCSC	uc002gca.1. human.
Organism-specific databases
CTD	56919.
GeneCards	GC17M005286.
HGNC	HGNC:16718. DHX33.
neXtProt	NX_Q9H6R0.
PharmGKB	PA27220.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10668.
HOVERGEN	HBG039428.
InParanoid	Q9H6R0.
OrthoDB	EOG46Q6S1.
PhylomeDB	Q9H6R0.
Gene expression databases
ArrayExpress	Q9H6R0.
Bgee	Q9H6R0.
CleanEx	HS_DHX33.
Genevestigator	Q9H6R0.
GermOnline	ENSG00000005100. Homo sapiens.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR011709. DUF1605. IPR007502. Helicase-assoc_dom. IPR001650. Helicase_C. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF04408. HA2. 1 hit. PF00271. Helicase_C. 1 hit. PF07717. OB_NTP_bind. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00847. HA2. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	62420.

Entry information

Entry name

DHX33_HUMAN

Accession

Primary (citable) accession number: Q9H6R0
Secondary accession number(s): B4DHF9 , Q4G149, Q5CZ73, Q9H5M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.