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Q9H6R0

- DHX33_HUMAN

UniProt

Q9H6R0 - DHX33_HUMAN

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Protein
Putative ATP-dependent RNA helicase DHX33
Gene
DHX33, DDX33
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. Important element of nucleolar organization.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi97 – 1048ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. poly(A) RNA binding Source: UniProtKB
  4. rDNA binding Source: UniProtKB

GO - Biological processi

  1. positive regulation of transcription from RNA polymerase I promoter Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase DHX33 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 33
Gene namesi
Name:DHX33
Synonyms:DDX33
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:16718. DHX33.

Subcellular locationi

Nucleusnucleolus. Nucleusnucleoplasm
Note: Predominantly in the nucleolus. During mitosis, localizes with the nucleolar organizing regions.2 Publications

GO - Cellular componenti

  1. nucleolus Source: UniProtKB
  2. nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27220.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Putative ATP-dependent RNA helicase DHX33
PRO_0000055164Add
BLAST

Proteomic databases

MaxQBiQ9H6R0.
PaxDbiQ9H6R0.
PRIDEiQ9H6R0.

PTM databases

PhosphoSiteiQ9H6R0.

Expressioni

Gene expression databases

ArrayExpressiQ9H6R0.
BgeeiQ9H6R0.
CleanExiHS_DHX33.
GenevestigatoriQ9H6R0.

Interactioni

Subunit structurei

Interacts with UBTF.1 Publication

Protein-protein interaction databases

BioGridi121247. 7 interactions.
IntActiQ9H6R0. 2 interactions.
MINTiMINT-3068519.
STRINGi9606.ENSP00000225296.

Structurei

3D structure databases

ProteinModelPortaliQ9H6R0.
SMRiQ9H6R0. Positions 71-698.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini84 – 252169Helicase ATP-binding
Add
BLAST
Domaini277 – 450174Helicase C-terminal
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi194 – 1974DEAH box
Motifi547 – 55812Critical for rDNA-binding By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1643.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ9H6R0.
KOiK17820.
OMAiGDMESVR.
OrthoDBiEOG718KC0.
PhylomeDBiQ9H6R0.
TreeFamiTF354245.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9H6R0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPEEAGFPPA KRFRPGSGPP SRAGSFPPGR QVVMLLTAGS GGRGGGGGRR    50
QQPPLAQPSA SPYPEAVELQ RRSLPIFQAR GQLLAQLRNL DNAVLIGETG 100
SGKTTQIPQY LYEGGISRQG IIAVTQPRRV AAISLATRVS DEKRTELGKL 150
VGYTVRFDDV TSEDTRIKFL TDGMLLREAI SDSLLRKYSC VILDEAHERT 200
IHTDVLFGVV KAAQKRRKEL GKLPLKVIVM SATMDVDLFS QYFNGAPVLY 250
LEGRQHPIQV FYTKQPQNDY LHAALVSVFQ IHQEAPSSQD ILVFLTGQEE 300
IEAMSKTCRD IAKHLPDGCP AMLVLPLYAS LPYAQQLRVF QGAPKGYRKV 350
IISTNIAETS ITITGIKYVV DTGMVKAKKY NPDSGLEVLA VQRVSKTQAW 400
QRTGRAGRED SGICYRLYTE DEFEKFDKMT VPEIQRCNLA SVMLQLLAMK 450
VPNVLTFDFM SKPSPDHIQA AIAQLDLLGA LEHKDDQLTL TPMGRKMAAF 500
PLEPKFAKTI LMSPKFHCTE EILTIVSLLS VDSVLHNPPS RREEVQGVRK 550
KFISSEGDHM TLLNIYRTFK NLGGNKDWCK ENFVNSKNMT LVAEVRAQLR 600
DICLKMSMPI ASSRGDVESV RRCLAHSLFM STAELQPDGT YATTDTHQPV 650
AIHPSSVLFH CKPACVVYTE LLYTNKCYMR DLCVIDAQWL YEAAPEYFRR 700
KLRTARN 707
Length:707
Mass (Da):78,874
Last modified:May 18, 2010 - v2
Checksum:iE3515B12BD972431
GO
Isoform 2 (identifier: Q9H6R0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-173: Missing.

Show »
Length:534
Mass (Da):60,393
Checksum:iA82774C3D3FC205E
GO

Sequence cautioni

The sequence AAH30017.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti118 – 1181R → C.
Corresponds to variant rs8069315 [ dbSNP | Ensembl ].
VAR_057239
Natural varianti483 – 4831H → D.
Corresponds to variant rs11653658 [ dbSNP | Ensembl ].
VAR_057240

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 173173Missing in isoform 2.
VSP_016256Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771F → L in BAB15193. 1 Publication
Sequence conflicti166 – 1661R → G in BAB15193. 1 Publication
Sequence conflicti377 – 3771A → V in AAH30017. 1 Publication
Sequence conflicti539 – 5391P → S in CAI56793. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK025625 mRNA. Translation: BAB15193.1.
AK026944 mRNA. Translation: BAB15596.1.
AK295074 mRNA. Translation: BAG58120.1.
AC004148 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90332.1.
BC030017 mRNA. Translation: AAH30017.1. Different initiation.
CR936655 mRNA. Translation: CAI56793.1.
CCDSiCCDS11072.1. [Q9H6R0-1]
RefSeqiNP_001186628.1. NM_001199699.1. [Q9H6R0-2]
NP_064547.2. NM_020162.3. [Q9H6R0-1]
UniGeneiHs.250456.

Genome annotation databases

EnsembliENST00000225296; ENSP00000225296; ENSG00000005100. [Q9H6R0-1]
GeneIDi56919.
KEGGihsa:56919.
UCSCiuc002gbz.3. human. [Q9H6R0-1]

Polymorphism databases

DMDMi296434478.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK025625 mRNA. Translation: BAB15193.1 .
AK026944 mRNA. Translation: BAB15596.1 .
AK295074 mRNA. Translation: BAG58120.1 .
AC004148 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90332.1 .
BC030017 mRNA. Translation: AAH30017.1 . Different initiation.
CR936655 mRNA. Translation: CAI56793.1 .
CCDSi CCDS11072.1. [Q9H6R0-1 ]
RefSeqi NP_001186628.1. NM_001199699.1. [Q9H6R0-2 ]
NP_064547.2. NM_020162.3. [Q9H6R0-1 ]
UniGenei Hs.250456.

3D structure databases

ProteinModelPortali Q9H6R0.
SMRi Q9H6R0. Positions 71-698.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121247. 7 interactions.
IntActi Q9H6R0. 2 interactions.
MINTi MINT-3068519.
STRINGi 9606.ENSP00000225296.

PTM databases

PhosphoSitei Q9H6R0.

Polymorphism databases

DMDMi 296434478.

Proteomic databases

MaxQBi Q9H6R0.
PaxDbi Q9H6R0.
PRIDEi Q9H6R0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000225296 ; ENSP00000225296 ; ENSG00000005100 . [Q9H6R0-1 ]
GeneIDi 56919.
KEGGi hsa:56919.
UCSCi uc002gbz.3. human. [Q9H6R0-1 ]

Organism-specific databases

CTDi 56919.
GeneCardsi GC17M005360.
HGNCi HGNC:16718. DHX33.
MIMi 614405. gene.
neXtProti NX_Q9H6R0.
PharmGKBi PA27220.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1643.
HOGENOMi HOG000175261.
HOVERGENi HBG039428.
InParanoidi Q9H6R0.
KOi K17820.
OMAi GDMESVR.
OrthoDBi EOG718KC0.
PhylomeDBi Q9H6R0.
TreeFami TF354245.

Miscellaneous databases

ChiTaRSi DHX33. human.
GenomeRNAii 56919.
NextBioi 62420.
PROi Q9H6R0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9H6R0.
Bgeei Q9H6R0.
CleanExi HS_DHX33.
Genevestigatori Q9H6R0.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Hepatoma.
  2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  5. Bienvenut W.V.
    Submitted (AUG-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 119-128; 130-138 AND 200-211, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Cervix carcinoma.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 293-707.
    Tissue: Melanoma.
  7. "Identification of DHX33 as a mediator of rRNA synthesis and cell growth."
    Zhang Y., Forys J.T., Miceli A.P., Gwinn A.S., Weber J.D.
    Mol. Cell. Biol. 31:4676-4691(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH RIBOSOMAL DNA LOCI, INTERACTION WITH UBTF, SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDHX33_HUMAN
AccessioniPrimary (citable) accession number: Q9H6R0
Secondary accession number(s): B4DHF9
, Q4G149, Q5CZ73, Q9H5M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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