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Protein

Cytosolic Fe-S cluster assembly factor NARFL

Gene

NARFL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi24Iron-sulfur 1 (4Fe-4S)Sequence analysis1
Metal bindingi71Iron-sulfur 1 (4Fe-4S)Sequence analysis1
Metal bindingi74Iron-sulfur 1 (4Fe-4S)Sequence analysis1
Metal bindingi77Iron-sulfur 1 (4Fe-4S)Sequence analysis1
Metal bindingi190Iron-sulfur 2 (4Fe-4S)Sequence analysis1
Metal bindingi246Iron-sulfur 2 (4Fe-4S)Sequence analysis1
Metal bindingi395Iron-sulfur 2 (4Fe-4S)Sequence analysis1
Metal bindingi399Iron-sulfur 2 (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • hematopoietic progenitor cell differentiation Source: Ensembl
  • iron-sulfur cluster assembly Source: UniProtKB
  • oxygen homeostasis Source: HGNC
  • regulation of gene expression Source: BHF-UCL
  • response to hypoxia Source: HGNC
Complete GO annotation...

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-2564830. Cytosolic iron-sulfur cluster assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NARFL
Alternative name(s):
Iron-only hydrogenase-like protein 1
Short name:
IOP1
Nuclear prelamin A recognition factor-like protein
Protein related to Narf
Gene namesi
Name:NARFL
Synonyms:PRN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:14179. NARFL.

Subcellular locationi

GO - Cellular componenti

  • CIA complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi64428.
OpenTargetsiENSG00000103245.
PharmGKBiPA128394707.

Polymorphism and mutation databases

BioMutaiNARFL.
DMDMi74733617.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002884852 – 476Cytosolic Fe-S cluster assembly factor NARFLAdd BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9H6Q4.
MaxQBiQ9H6Q4.
PaxDbiQ9H6Q4.
PeptideAtlasiQ9H6Q4.
PRIDEiQ9H6Q4.

PTM databases

iPTMnetiQ9H6Q4.
PhosphoSitePlusiQ9H6Q4.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000103245.
CleanExiHS_NARFL.
ExpressionAtlasiQ9H6Q4. baseline and differential.
GenevisibleiQ9H6Q4. HS.

Organism-specific databases

HPAiHPA040851.

Interactioni

Subunit structurei

Component of the CIA complex.2 Publications

Protein-protein interaction databases

BioGridi122176. 13 interactors.
IntActiQ9H6Q4. 1 interactor.
STRINGi9606.ENSP00000251588.

Structurei

3D structure databases

ProteinModelPortaliQ9H6Q4.
SMRiQ9H6Q4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NARF family.Curated

Phylogenomic databases

eggNOGiKOG2439. Eukaryota.
COG4624. LUCA.
GeneTreeiENSGT00390000017231.
HOGENOMiHOG000191744.
HOVERGENiHBG055005.
InParanoidiQ9H6Q4.
OMAiFIGPSQD.
OrthoDBiEOG091G0BWP.
PhylomeDBiQ9H6Q4.
TreeFamiTF106273.

Family and domain databases

Gene3Di4.10.260.20. 1 hit.
InterProiIPR009016. Fe_hydrogenase.
IPR004108. Fe_hydrogenase_lsu_C.
IPR003149. Fe_hydrogenase_ssu.
[Graphical view]
PfamiPF02906. Fe_hyd_lg_C. 1 hit.
PF02256. Fe_hyd_SSU. 1 hit.
[Graphical view]
SMARTiSM00902. Fe_hyd_SSU. 1 hit.
[Graphical view]
SUPFAMiSSF53920. SSF53920. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H6Q4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPFSGALQ LTDLDDFIGP SQECIKPVKV EKRAGSGVAK IRIEDDGSYF
60 70 80 90 100
QINQDGGTRR LEKAKVSLND CLACSGCITS AETVLITQQS HEELKKVLDA
110 120 130 140 150
NKMAAPSQQR LVVVSVSPQS RASLAARFQL NPTDTARKLT SFFKKIGVHF
160 170 180 190 200
VFDTAFSRHF SLLESQREFV RRFRGQADCR QALPLLASAC PGWICYAEKT
210 220 230 240 250
HGSFILPHIS TARSPQQVMG SLVKDFFAQQ QHLTPDKIYH VTVMPCYDKK
260 270 280 290 300
LEASRPDFFN QEHQTRDVDC VLTTGEVFRL LEEEGVSLPD LEPAPLDSLC
310 320 330 340 350
SGASAEEPTS HRGGGSGGYL EHVFRHAARE LFGIHVAEVT YKPLRNKDFQ
360 370 380 390 400
EVTLEKEGQV LLHFAMAYGF RNIQNLVQRL KRGRCPYHYV EVMACPSGCL
410 420 430 440 450
NGGGQLQAPD RPSRELLQHV ERLYGMVRAE APEDAPGVQE LYTHWLQGTD
460 470
SECAGRLLHT QYHAVEKAST GLGIRW
Length:476
Mass (Da):53,020
Last modified:March 1, 2001 - v1
Checksum:i88A019DA4D7988EA
GO
Isoform 2 (identifier: Q9H6Q4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-243: Missing.

Show »
Length:233
Mass (Da):26,135
Checksum:iAA85FD01731E7051
GO
Isoform 3 (identifier: Q9H6Q4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.

Show »
Length:374
Mass (Da):42,010
Checksum:iD22B5E1FFD155545
GO

Sequence cautioni

The sequence AAK61251 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti205I → T in BAD96725 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05391138V → M.Corresponds to variant rs8045850dbSNPEnsembl.1
Natural variantiVAR_053912444H → R.Corresponds to variant rs7188554dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0256941 – 243Missing in isoform 2. 1 PublicationAdd BLAST243
Alternative sequenceiVSP_0256951 – 102Missing in isoform 3. 1 PublicationAdd BLAST102

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY129231 mRNA. Translation: AAM98737.1.
AK025641 mRNA. Translation: BAB15199.1.
AK025861 mRNA. Translation: BAB15261.1.
AK095675 mRNA. Translation: BAG53105.1.
AK223005 mRNA. Translation: BAD96725.1.
AE006464 Genomic DNA. Translation: AAK61251.1. Sequence problems.
Z98258 Genomic DNA. Translation: CAM26480.1.
CH471112 Genomic DNA. Translation: EAW85723.1.
BC030248 mRNA. Translation: AAH30248.1.
BC129940 mRNA. Translation: AAI29941.1.
CCDSiCCDS10425.1. [Q9H6Q4-1]
CCDS76798.1. [Q9H6Q4-3]
RefSeqiNP_001291728.1. NM_001304799.1. [Q9H6Q4-3]
NP_071938.1. NM_022493.2. [Q9H6Q4-1]
UniGeneiHs.513247.

Genome annotation databases

EnsembliENST00000251588; ENSP00000251588; ENSG00000103245. [Q9H6Q4-1]
ENST00000540986; ENSP00000444008; ENSG00000103245. [Q9H6Q4-3]
ENST00000568545; ENSP00000457058; ENSG00000103245. [Q9H6Q4-3]
GeneIDi64428.
KEGGihsa:64428.
UCSCiuc002cjp.4. human. [Q9H6Q4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY129231 mRNA. Translation: AAM98737.1.
AK025641 mRNA. Translation: BAB15199.1.
AK025861 mRNA. Translation: BAB15261.1.
AK095675 mRNA. Translation: BAG53105.1.
AK223005 mRNA. Translation: BAD96725.1.
AE006464 Genomic DNA. Translation: AAK61251.1. Sequence problems.
Z98258 Genomic DNA. Translation: CAM26480.1.
CH471112 Genomic DNA. Translation: EAW85723.1.
BC030248 mRNA. Translation: AAH30248.1.
BC129940 mRNA. Translation: AAI29941.1.
CCDSiCCDS10425.1. [Q9H6Q4-1]
CCDS76798.1. [Q9H6Q4-3]
RefSeqiNP_001291728.1. NM_001304799.1. [Q9H6Q4-3]
NP_071938.1. NM_022493.2. [Q9H6Q4-1]
UniGeneiHs.513247.

3D structure databases

ProteinModelPortaliQ9H6Q4.
SMRiQ9H6Q4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122176. 13 interactors.
IntActiQ9H6Q4. 1 interactor.
STRINGi9606.ENSP00000251588.

PTM databases

iPTMnetiQ9H6Q4.
PhosphoSitePlusiQ9H6Q4.

Polymorphism and mutation databases

BioMutaiNARFL.
DMDMi74733617.

Proteomic databases

EPDiQ9H6Q4.
MaxQBiQ9H6Q4.
PaxDbiQ9H6Q4.
PeptideAtlasiQ9H6Q4.
PRIDEiQ9H6Q4.

Protocols and materials databases

DNASUi64428.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000251588; ENSP00000251588; ENSG00000103245. [Q9H6Q4-1]
ENST00000540986; ENSP00000444008; ENSG00000103245. [Q9H6Q4-3]
ENST00000568545; ENSP00000457058; ENSG00000103245. [Q9H6Q4-3]
GeneIDi64428.
KEGGihsa:64428.
UCSCiuc002cjp.4. human. [Q9H6Q4-1]

Organism-specific databases

CTDi64428.
DisGeNETi64428.
GeneCardsiNARFL.
HGNCiHGNC:14179. NARFL.
HPAiHPA040851.
MIMi611118. gene.
neXtProtiNX_Q9H6Q4.
OpenTargetsiENSG00000103245.
PharmGKBiPA128394707.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2439. Eukaryota.
COG4624. LUCA.
GeneTreeiENSGT00390000017231.
HOGENOMiHOG000191744.
HOVERGENiHBG055005.
InParanoidiQ9H6Q4.
OMAiFIGPSQD.
OrthoDBiEOG091G0BWP.
PhylomeDBiQ9H6Q4.
TreeFamiTF106273.

Enzyme and pathway databases

ReactomeiR-HSA-2564830. Cytosolic iron-sulfur cluster assembly.

Miscellaneous databases

ChiTaRSiNARFL. human.
GenomeRNAii64428.
PROiQ9H6Q4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103245.
CleanExiHS_NARFL.
ExpressionAtlasiQ9H6Q4. baseline and differential.
GenevisibleiQ9H6Q4. HS.

Family and domain databases

Gene3Di4.10.260.20. 1 hit.
InterProiIPR009016. Fe_hydrogenase.
IPR004108. Fe_hydrogenase_lsu_C.
IPR003149. Fe_hydrogenase_ssu.
[Graphical view]
PfamiPF02906. Fe_hyd_lg_C. 1 hit.
PF02256. Fe_hyd_SSU. 1 hit.
[Graphical view]
SMARTiSM00902. Fe_hyd_SSU. 1 hit.
[Graphical view]
SUPFAMiSSF53920. SSF53920. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNARFL_HUMAN
AccessioniPrimary (citable) accession number: Q9H6Q4
Secondary accession number(s): A1L385
, B3KTJ3, Q53GC6, Q96S10, Q9H6J8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.