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Q9H6Q4 (NARFL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosolic Fe-S cluster assembly factor NARFL
Alternative name(s):
Iron-only hydrogenase-like protein 1
Short name=IOP1
Nuclear prelamin A recognition factor-like protein
Protein related to Narf
Gene names
Name:NARFL
Synonyms:PRN
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. Ref.8 Ref.9

Subunit structure

Component of the CIA complex. Ref.12 Ref.13

Tissue specificity

Widely expressed. Ref.8

Sequence similarities

Belongs to the NARF family.

Sequence caution

The sequence AAK61251.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H6Q4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H6Q4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-243: Missing.
Isoform 3 (identifier: Q9H6Q4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.10
Chain2 – 476475Cytosolic Fe-S cluster assembly factor NARFL
PRO_0000288485

Sites

Metal binding241Iron-sulfur 1 (4Fe-4S) Potential
Metal binding711Iron-sulfur 1 (4Fe-4S) Potential
Metal binding741Iron-sulfur 1 (4Fe-4S) Potential
Metal binding771Iron-sulfur 1 (4Fe-4S) Potential
Metal binding1901Iron-sulfur 2 (4Fe-4S) Potential
Metal binding2461Iron-sulfur 2 (4Fe-4S) Potential
Metal binding3951Iron-sulfur 2 (4Fe-4S) Potential
Metal binding3991Iron-sulfur 2 (4Fe-4S) Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.10

Natural variations

Alternative sequence1 – 243243Missing in isoform 2.
VSP_025694
Alternative sequence1 – 102102Missing in isoform 3.
VSP_025695
Natural variant381V → M.
Corresponds to variant rs8045850 [ dbSNP | Ensembl ].
VAR_053911
Natural variant4441H → R.
Corresponds to variant rs7188554 [ dbSNP | Ensembl ].
VAR_053912

Experimental info

Sequence conflict2051I → T in BAD96725. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 88A019DA4D7988EA

FASTA47653,020
        10         20         30         40         50         60 
MASPFSGALQ LTDLDDFIGP SQECIKPVKV EKRAGSGVAK IRIEDDGSYF QINQDGGTRR 

        70         80         90        100        110        120 
LEKAKVSLND CLACSGCITS AETVLITQQS HEELKKVLDA NKMAAPSQQR LVVVSVSPQS 

       130        140        150        160        170        180 
RASLAARFQL NPTDTARKLT SFFKKIGVHF VFDTAFSRHF SLLESQREFV RRFRGQADCR 

       190        200        210        220        230        240 
QALPLLASAC PGWICYAEKT HGSFILPHIS TARSPQQVMG SLVKDFFAQQ QHLTPDKIYH 

       250        260        270        280        290        300 
VTVMPCYDKK LEASRPDFFN QEHQTRDVDC VLTTGEVFRL LEEEGVSLPD LEPAPLDSLC 

       310        320        330        340        350        360 
SGASAEEPTS HRGGGSGGYL EHVFRHAARE LFGIHVAEVT YKPLRNKDFQ EVTLEKEGQV 

       370        380        390        400        410        420 
LLHFAMAYGF RNIQNLVQRL KRGRCPYHYV EVMACPSGCL NGGGQLQAPD RPSRELLQHV 

       430        440        450        460        470 
ERLYGMVRAE APEDAPGVQE LYTHWLQGTD SECAGRLLHT QYHAVEKAST GLGIRW 

« Hide

Isoform 2 [UniParc].

Checksum: AA85FD01731E7051
Show »

FASTA23326,135
Isoform 3 [UniParc].

Checksum: D22B5E1FFD155545
Show »

FASTA37442,010

References

« Hide 'large scale' references
[1]"A comparison of Narf, HPRN and Nar1p in human and yeast cells."
Barton R.M., Worman H.J.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Brain.
[3]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Small intestine.
[4]"Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16."
Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R.
Hum. Mol. Genet. 10:339-352(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.
[8]"IOP1, a novel hydrogenase-like protein that modulates hypoxia-inducible factor-1alpha activity."
Huang J., Song D., Flores A., Zhao Q., Mooney S.M., Shaw L.M., Lee F.S.
Biochem. J. 401:341-352(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[9]"A role for IOP1 in mammalian cytosolic iron-sulfur protein biogenesis."
Song D., Lee F.S.
J. Biol. Chem. 283:9231-9238(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity."
Stehling O., Vashisht A.A., Mascarenhas J., Jonsson Z.O., Sharma T., Netz D.J., Pierik A.J., Wohlschlegel J.A., Lill R.
Science 337:195-199(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CIA COMPLEX.
[13]"MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism."
Gari K., Leon Ortiz A.M., Borel V., Flynn H., Skehel J.M., Boulton S.J.
Science 337:243-245(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CIA COMPLEX.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY129231 mRNA. Translation: AAM98737.1.
AK025641 mRNA. Translation: BAB15199.1.
AK025861 mRNA. Translation: BAB15261.1.
AK095675 mRNA. Translation: BAG53105.1.
AK223005 mRNA. Translation: BAD96725.1.
AE006464 Genomic DNA. Translation: AAK61251.1. Sequence problems.
Z98258 Genomic DNA. Translation: CAM26480.1.
CH471112 Genomic DNA. Translation: EAW85723.1.
BC030248 mRNA. Translation: AAH30248.1.
BC129940 mRNA. Translation: AAI29941.1.
RefSeqNP_071938.1. NM_022493.1.
XP_005255557.1. XM_005255500.1.
UniGeneHs.513247.

3D structure databases

ProteinModelPortalQ9H6Q4.
SMRQ9H6Q4. Positions 70-464.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122176. 4 interactions.
STRING9606.ENSP00000251588.

PTM databases

PhosphoSiteQ9H6Q4.

Polymorphism databases

DMDM74733617.

Proteomic databases

PaxDbQ9H6Q4.
PeptideAtlasQ9H6Q4.
PRIDEQ9H6Q4.

Protocols and materials databases

DNASU64428.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000251588; ENSP00000251588; ENSG00000103245. [Q9H6Q4-1]
ENST00000540986; ENSP00000444008; ENSG00000103245. [Q9H6Q4-3]
ENST00000568545; ENSP00000457058; ENSG00000103245. [Q9H6Q4-3]
GeneID64428.
KEGGhsa:64428.
UCSCuc002cjp.3. human. [Q9H6Q4-1]

Organism-specific databases

CTD64428.
GeneCardsGC16M000794.
HGNCHGNC:14179. NARFL.
HPAHPA040851.
MIM611118. gene.
neXtProtNX_Q9H6Q4.
PharmGKBPA128394707.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG4624.
HOGENOMHOG000191744.
HOVERGENHBG055005.
OMAPQALMGT.
OrthoDBEOG7VB2FK.
PhylomeDBQ9H6Q4.
TreeFamTF106273.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressQ9H6Q4.
BgeeQ9H6Q4.
CleanExHS_NARFL.
GenevestigatorQ9H6Q4.

Family and domain databases

Gene3D4.10.260.20. 1 hit.
InterProIPR009016. Fe_hydrogenase.
IPR004108. Fe_hydrogenase_lsu_C.
IPR003149. Fe_hydrogenase_ssu-like.
[Graphical view]
PfamPF02906. Fe_hyd_lg_C. 1 hit.
PF02256. Fe_hyd_SSU. 1 hit.
[Graphical view]
SMARTSM00902. Fe_hyd_SSU. 1 hit.
[Graphical view]
SUPFAMSSF53920. SSF53920. 2 hits.
ProtoNetSearch...

Other

GenomeRNAi64428.
NextBio66437.
PROQ9H6Q4.
SOURCESearch...

Entry information

Entry nameNARFL_HUMAN
AccessionPrimary (citable) accession number: Q9H6Q4
Secondary accession number(s): A1L385 expand/collapse secondary AC list , B3KTJ3, Q53GC6, Q96S10, Q9H6J8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM