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Q9H6D3 (XKR8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
XK-related protein 8

Short name=hXkr8

Cleaved into the following chain:

  1. XK-related protein 8, processed form
Gene names
Name:XKR8
Synonyms:XRG8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes phosphatidylserine exposure on apoptotic cell surface, possibly by mediating phospholipid scrambling. Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment. Has no effect on calcium-induced exposure of phosphatidylserine. Activated upon caspase cleavage, suggesting that it does not act prior the onset of apoptosis. Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.6.

Post-translational modification

Undergoes proteolytic processing by caspase-3 (CASP3), leading to its activation.

Miscellaneous

Expression is repressed in PLB-985 leukemia and Raji lymphoma cells due to CpG methylation near the transcription start site of XKR8 gene, possibly explaining the inability of PLB-985 leukemia and Raji lymphoma cells to expose phosphatidylserine during apoptosis (Ref.6).

Sequence similarities

Belongs to the XK family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395XK-related protein 8
PRO_0000190792
Chain1 – 355355XK-related protein 8, processed form Potential
PRO_0000423984

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3321Helical; Potential
Topological domain34 – 4714Extracellular Potential
Transmembrane48 – 6821Helical; Potential
Topological domain69 – 15890Cytoplasmic Potential
Transmembrane159 – 17921Helical; Potential
Topological domain180 – 20021Extracellular Potential
Transmembrane201 – 22121Helical; Potential
Topological domain222 – 2232Cytoplasmic Potential
Transmembrane224 – 24421Helical; Potential
Topological domain245 – 25915Extracellular Potential
Transmembrane260 – 27718Helical; Potential
Topological domain278 – 2847Cytoplasmic Potential
Transmembrane285 – 30521Helical; Potential
Topological domain306 – 3127Extracellular Potential
Transmembrane313 – 33321Helical; Potential
Topological domain334 – 39562Cytoplasmic Potential

Sites

Site355 – 3562Cleavage; by caspase-3 Potential

Experimental info

Mutagenesis352 – 3554DQVD → AQVA: Abolishes cleavage by CASP3 and ability to promote phosphatidylserine exposure. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q9H6D3 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 23199BAEEA6964C6

FASTA39544,655
        10         20         30         40         50         60 
MPWSSRGALL RDLVLGVLGT AAFLLDLGTD LWAAVQYALG GRYLWAALVL ALLGLASVAL 

        70         80         90        100        110        120 
QLFSWLWLRA DPAGLHGSQP PRRCLALLHL LQLGYLYRCV QELRQGLLVW QQEEPSEFDL 

       130        140        150        160        170        180 
AYADFLALDI SMLRLFETFL ETAPQLTLVL AIMLQSGRAE YYQWVGICTS FLGISWALLD 

       190        200        210        220        230        240 
YHRALRTCLP SKPLLGLGSS VIYFLWNLLL LWPRVLAVAL FSALFPSYVA LHFLGLWLVL 

       250        260        270        280        290        300 
LLWVWLQGTD FMPDPSSEWL YRVTVATILY FSWFNVAEGR TRGRAIIHFA FLLSDSILLV 

       310        320        330        340        350        360 
ATWVTHSSWL PSGIPLQLWL PVGCGCFFLG LALRLVYYHW LHPSCCWKPD PDQVDGARSL 

       370        380        390 
LSPEGYQLPQ NRRMTHLAQK FFPKAKDEAA SPVKG 

« Hide

References

« Hide 'large scale' references
[1]"A superfamily of XK-related genes (XRG) widely expressed in vertebrates and invertebrates."
Huang C.-H., Chen Y.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[5]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[6]"Xk-Related protein 8 and CED-8 promote phosphatidylserine exposure in apoptotic cells."
Suzuki J., Denning D.P., Imanishi E., Horvitz H.R., Nagata S.
Science 341:403-406(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, PROTEOLYTIC PROCESSING BY CASP3, MUTAGENESIS OF 352-ASP--ASP-355.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY534246 mRNA. Translation: AAT07095.1.
AK026024 mRNA. Translation: BAB15326.1.
AL512288 Genomic DNA. Translation: CAI14292.1.
BC013379 mRNA. Translation: AAH13379.1.
BC028564 mRNA. Translation: AAH28564.1.
RefSeqNP_060523.2. NM_018053.2.
UniGeneHs.55024.

3D structure databases

ProteinModelPortalQ9H6D3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4723120.
STRING9606.ENSP00000362991.

Polymorphism databases

DMDM74752693.

Proteomic databases

PaxDbQ9H6D3.
PRIDEQ9H6D3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000373884; ENSP00000362991; ENSG00000158156.
GeneID55113.
KEGGhsa:55113.
UCSCuc001bph.1. human.

Organism-specific databases

CTD55113.
GeneCardsGC01P028285.
HGNCHGNC:25508. XKR8.
HPAHPA049984.
neXtProtNX_Q9H6D3.
PharmGKBPA142670566.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG258849.
HOGENOMHOG000082540.
HOVERGENHBG055840.
InParanoidQ9H6D3.
OMAFPHYVAL.
OrthoDBEOG7T4MKZ.
PhylomeDBQ9H6D3.
TreeFamTF316454.

Gene expression databases

ArrayExpressQ9H6D3.
BgeeQ9H6D3.
CleanExHS_XKR8.
GenevestigatorQ9H6D3.

Family and domain databases

InterProIPR018629. Transport_prot_XK.
[Graphical view]
PfamPF09815. XK-related. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi55113.
NextBio58740.
PROQ9H6D3.

Entry information

Entry nameXKR8_HUMAN
AccessionPrimary (citable) accession number: Q9H6D3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: March 1, 2001
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM