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Protein

Epoxide hydrolase 3

Gene

EPHX3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of epoxide-containing fatty acids. Active in vitro against epoxyeicosatrienoic acids (EETs) including 8,9-EET, 9,10-EET, 11,12-EET and 14,15-EET and leukotoxin.1 Publication

Catalytic activityi

An epoxide + H2O = a glycol.1 Publication

Enzyme regulationi

Inhibited by 1-(1-acetylpiperidin-4-yl)-3-(4-(trifl uoromethoxy)phenyl)urea (TPAU), 1-cyclohexyl-3-dodecylurea (CDU), 12-(3-adamantan-1-yl-ureido)-dodecanoic acid (AUDA), 1-((3S, 5S, 7S)-adamantan-1-yl)-3-(5-(2-(2-ethoxyethoxy) ethoxy)pentyl)urea (AEPU) and to a lesser extent by 8-(3-((3S, 5S, 7S)-adamantan-1-yl)ureido) octanoic acid (AUOA).1 Publication

Kineticsi

  1. KM=30 µM for 8,9-EET1 Publication
  2. KM=80 µM for 11,12-EET1 Publication
  3. KM=130 µM for 14,15-EET1 Publication
  4. KM=25 µM for leukotoxin1 Publication
  1. Vmax=12 µmol/min/mg enzyme with 8,9-EET as substrate1 Publication
  2. Vmax=50 µmol/min/mg enzyme with 11,12-EET as substrate1 Publication
  3. Vmax=60 µmol/min/mg enzyme with 14,15-EET as substrate1 Publication
  4. Vmax=22 µmol/min/mg enzyme with leukotoxin as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173NucleophileBy similarity1
Active sitei281Proton donorBy similarity1
Active sitei337Proton acceptorBy similarity1

GO - Molecular functioni

  • epoxide hydrolase activity Source: UniProtKB

GO - Biological processi

  • epoxide metabolic process Source: UniProtKB
  • lipid metabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism

Protein family/group databases

ESTHERihuman-EPHX3 Epoxide_hydrolase
MEROPSiS33.978

Chemistry databases

SwissLipidsiSLP:000001116

Names & Taxonomyi

Protein namesi
Recommended name:
Epoxide hydrolase 3 (EC:3.3.2.101 Publication)
Short name:
EH31 Publication
Alternative name(s):
Abhydrolase domain-containing protein 9
Gene namesi
Name:EPHX3
Synonyms:ABHD9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105131.7
HGNCiHGNC:23760 EPHX3
MIMi617400 gene
neXtProtiNX_Q9H6B9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173D → A: Loss of catalytic activity. 1 Publication1
Mutagenesisi173D → N: No effect on catalytic activity. 1 Publication1
Mutagenesisi220Y → F: Loss of catalytic activity. 1 Publication1
Mutagenesisi280Y → F: No effect on catalytic activity. 1 Publication1
Mutagenesisi281Y → F: Loss of catalytic activity. 1 Publication1
Mutagenesisi307D → A or N: Loss of catalytic activity. 1 Publication1
Mutagenesisi337H → Q: Loss of catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi79852
OpenTargetsiENSG00000105131
PharmGKBiPA164719188

Polymorphism and mutation databases

BioMutaiEPHX3
DMDMi74718486

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002642321 – 360Epoxide hydrolase 3Add BLAST360

Proteomic databases

EPDiQ9H6B9
MaxQBiQ9H6B9
PaxDbiQ9H6B9
PeptideAtlasiQ9H6B9
PRIDEiQ9H6B9

PTM databases

iPTMnetiQ9H6B9
PhosphoSitePlusiQ9H6B9

Expressioni

Gene expression databases

BgeeiENSG00000105131
CleanExiHS_ABHD9
ExpressionAtlasiQ9H6B9 baseline and differential
GenevisibleiQ9H6B9 HS

Organism-specific databases

HPAiHPA012842

Structurei

3D structure databases

ProteinModelPortaliQ9H6B9
SMRiQ9H6B9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410INN9 Eukaryota
ENOG410XQGI LUCA
GeneTreeiENSGT00530000063213
HOGENOMiHOG000028073
HOVERGENiHBG099190
InParanoidiQ9H6B9
KOiK22368
OMAiTTLTHRK
OrthoDBiEOG091G0ASI
PhylomeDBiQ9H6B9
TreeFamiTF314403

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000073 AB_hydrolase_1
IPR000639 Epox_hydrolase-like
PfamiView protein in Pfam
PF12697 Abhydrolase_6, 1 hit
PRINTSiPR00111 ABHYDROLASE
PR00412 EPOXHYDRLASE
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

Q9H6B9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPELVVTALL APSRLSLKLL RAFMWSLVFS VALVAAAVYG CIALTHVLCR
60 70 80 90 100
PRRGCCGRRR SASPACLSDP SLGEHGFLNL KSSGLRLHYV SAGRGNGPLM
110 120 130 140 150
LFLHGFPENW FSWRYQLREF QSRFHVVAVD LRGYGPSDAP RDVDCYTIDL
160 170 180 190 200
LLVDIKDVIL GLGYSKCILV AHDWGALLAW HFSIYYPSLV ERMVVVSGAP
210 220 230 240 250
MSVYQDYSLH HISQFFRSHY MFLFQLPWLP EKLLSMSDFQ ILKTTLTHRK
260 270 280 290 300
TGIPCLTPSE LEAFLYNFSQ PGGLTGPLNY YRNLFRNFPL EPQELTTPTL
310 320 330 340 350
LLWGEKDTYL ELGLVEAIGS RFVPGRLEAH ILPGIGHWIP QSNPQEMHQY
360
MWAFLQDLLD
Length:360
Mass (Da):40,909
Last modified:March 1, 2001 - v1
Checksum:iC465A4B0E2D56BA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026061 mRNA Translation: BAB15342.1
CH471106 Genomic DNA Translation: EAW84467.1
BC115002 mRNA Translation: AAI15003.1
BC132958 mRNA Translation: AAI32959.1
BC132960 mRNA Translation: AAI32961.1
CCDSiCCDS12327.1
RefSeqiNP_001136358.1, NM_001142886.1
NP_079070.1, NM_024794.2
UniGeneiHs.156457

Genome annotation databases

EnsembliENST00000221730; ENSP00000221730; ENSG00000105131
ENST00000435261; ENSP00000410323; ENSG00000105131
ENST00000602233; ENSP00000469345; ENSG00000105131
GeneIDi79852
KEGGihsa:79852
UCSCiuc002nap.4 human

Similar proteinsi

Entry informationi

Entry nameiEPHX3_HUMAN
AccessioniPrimary (citable) accession number: Q9H6B9
Secondary accession number(s): A3KMR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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