Q9H668 (STN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CST complex subunit STN1

Alternative name(s):
Oligonucleotide/oligosaccharide-binding fold-containing protein 1
Suppressor of cdc thirteen homolog

Gene names

Name:	OBFC1
Synonyms:	STN1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Ref.5 Ref.6
Subunit structure	Component of the CST complex, composed of TEN1/C17orf106, CTC1/C17orf68 and STN1/OBFC1. Interacts with TEN1/C17orf106 and CTC1/C17orf68; the interaction is direct. Interacts with ACD/TPP1. Ref.5 Ref.6
Subcellular location	Nucleus. Chromosome › telomere Ref.5 Ref.6.
Sequence similarities	Belongs to the STN1 family. Contains 1 OB DNA-binding domain.

Ontologies

Keywords
Cellular component	Chromosome Nucleus Telomere
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of DNA replication Inferred from sequence or structural similarity. Source: UniProtKB telomere maintenance via telomere lengthening Inferred from mutant phenotype Ref.6. Source: UniProtKB
Cellular component	intermediate filament cytoskeleton Inferred from direct assay. Source: HPA
Molecular function	protein binding Inferred from physical interaction Ref.6Ref.5. Source: UniProtKB single-stranded telomeric DNA binding Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	CST complex subunit STN1		PRO_0000058020
Regions
	DNA binding	57 – 155	99	OB Ref.6
Natural variations
	Natural variant	151	1	T → A. Ref.1 Ref.4 Corresponds to variant rs2487999 [ dbSNP | Ensembl ].		VAR_022364
	Natural variant	248	1	S → C. Ref.1 Ref.4 Corresponds to variant rs10786775 [ dbSNP | Ensembl ].		VAR_022365

Sequences

	Sequence		Length	Mass (Da)
	Q9H668 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: 087E7EF75544A1F0	FASTA	368	42,119
	10 20 30 40 50 60 MQPGSSRCEE ETPSLLWGLD PVFLAFAKLY IRDILDMKES RQVPGVFLYN GHPIKQVDVL 70 80 90 100 110 120 GTVIGVRERD AFYSYGVDDS TGVINCICWK KLNTESVSAA PSAARELSLT SQLKKLQETI 130 140 150 160 170 180 EQKTKIEIGD TIRVRGSIRT YREEREIHAT TYYKVDDPVW NIQIARMLEL PTIYRKVYDQ 190 200 210 220 230 240 PFHSSALEKE EALSNPGALD LPSLTSLLSE KAKEFLMENR VQSFYQQELE MVESLLSLAN 250 260 270 280 290 300 QPVIHSASSD QVNFKKDTTS KAIHSIFKNA IQLLQEKGLV FQKDDGFDNL YYVTREDKDL 310 320 330 340 350 360 HRKIHRIIQQ DCQKPNHMEK GCHFLHILAC ARLSIRPGLS EAVLQQVLEL LEDQSDIVST MEHYYTAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ALA-151 AND CYS-248. Tissue: Small intestine.
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ALA-151 AND CYS-248. Tissue: Ovary.
[5]	"RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway." Miyake Y., Nakamura M., Nabetani A., Shimamura S., Tamura M., Yonehara S., Saito M., Ishikawa F. Mol. Cell 36:193-206(2009) [PubMed: 19854130] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE CST COMPLEX, SUBCELLULAR LOCATION, INTERACTION WITH C17ORF68 AND C17ORF106.
[6]	"OB fold-containing protein 1 (OBFC1), a human homolog of yeast Stn1, associates with TPP1 and is implicated in telomere length regulation." Wan M., Qin J., Songyang Z., Liu D. J. Biol. Chem. 284:26725-26731(2009) [PubMed: 19648609] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING, INTERACTION WITH ACD.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK026212 mRNA. Translation: BAB15396.1. AL133355 Genomic DNA. Translation: CAB81623.1. CH471066 Genomic DNA. Translation: EAW49619.1. CH471066 Genomic DNA. Translation: EAW49620.1. BC017400 mRNA. Translation: AAH17400.1.
IPI	IPI00554443.
RefSeq	NP_079204.2. NM_024928.4.
UniGene	Hs.62314.
3D structure databases
ProteinModelPortal	Q9H668.
SMR	Q9H668. Positions 195-301.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H668. 1 interaction.
MINT	MINT-1482105.
STRING	Q9H668.
Polymorphism databases
DMDM	62900737.
Proteomic databases
PRIDE	Q9H668.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000224950; ENSP00000224950; ENSG00000107960. ENST00000369764; ENSP00000358779; ENSG00000107960.
GeneID	79991.
KEGG	hsa:79991.
NMPDR	fig|9606.3.peg.4609.
UCSC	uc001kxl.1. human.
Organism-specific databases
CTD	79991.
GeneCards	GC10M105632.
HGNC	HGNC:26200. OBFC1.
HPA	HPA037924. HPA037925.
MIM	613128. gene.
neXtProt	NX_Q9H668.
PharmGKB	PA134987118.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07792.
GeneTree	ENSGT00390000000909.
HOGENOM	HBG714338.
HOVERGEN	HBG053364.
InParanoid	Q9H668.
OMA	AIHSIFK.
OrthoDB	EOG4H19W3.
PhylomeDB	Q9H668.
Gene expression databases
ArrayExpress	Q9H668.
Bgee	Q9H668.
CleanEx	HS_OBFC1.
Genevestigator	Q9H668.
GermOnline	ENSG00000107960. Homo sapiens.
Family and domain databases
InterPro	IPR014647. CST_STN1. IPR015253. DUF1879_CST_STN1. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF09170. DUF1879. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PIRSF	PIRSF036950. UCP036950. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...
Other
NextBio	70050.
SOURCE	Search...

Entry information

Entry name

STN1_HUMAN

Accession

Primary (citable) accession number: Q9H668
Secondary accession number(s): D3DR99, Q5TCZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.