Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CST complex subunit STN1

Gene

OBFC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22964711, PubMed:22763445). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins (PubMed:25483097). Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres (PubMed:22863775, PubMed:22964711). Promotes general replication start following replication-fork stalling implicating new origin firing (PubMed:22863775). May be in involved in C-strand fill-in during late S/G2 phase independent of ot its role in telomere duplex replication (PubMed:23142664).1 Publication7 Publications
Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi57 – 15599OBAdd
BLAST

GO - Molecular functioni

  • single-stranded DNA binding Source: UniProtKB
  • single-stranded telomeric DNA binding Source: UniProtKB
  • telomeric DNA binding Source: BHF-UCL

GO - Biological processi

  • negative regulation of telomerase activity Source: BHF-UCL
  • positive regulation of DNA replication Source: UniProtKB
  • positive regulation of telomerase activity Source: BHF-UCL
  • positive regulation of telomere maintenance via telomerase Source: BHF-UCL
  • regulation of telomere maintenance via telomere lengthening Source: BHF-UCL
  • telomere capping Source: InterPro
  • telomere maintenance Source: UniProtKB
  • telomere maintenance via telomere lengthening Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CST complex subunit STN1
Alternative name(s):
Oligonucleotide/oligosaccharide-binding fold-containing protein 1
Suppressor of cdc thirteen homolog
Gene namesi
Name:OBFC1
Synonyms:STN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:26200. OBFC1.

Subcellular locationi

GO - Cellular componenti

  • CST complex Source: BHF-UCL
  • intermediate filament cytoskeleton Source: HPA
  • intracellular membrane-bounded organelle Source: HPA
  • nuclear chromosome, telomeric region Source: UniProtKB
  • nucleolus Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi78 – 781D → A: Defective of TEN1 binding; when associated with ALA-164 or ALA-167. 1 Publication
Mutagenesisi164 – 1641I → A: Defective of TEN1 binding; when associated with ALA-78. 1 Publication
Mutagenesisi167 – 1671M → A: Defective of TEN1 binding; when associated with ALA-78. 1 Publication

Organism-specific databases

MalaCardsiOBFC1.
Orphaneti2032. Idiopathic pulmonary fibrosis.
PharmGKBiPA134987118.

Polymorphism and mutation databases

BioMutaiOBFC1.
DMDMi62900737.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368CST complex subunit STN1PRO_0000058020Add
BLAST

Proteomic databases

EPDiQ9H668.
MaxQBiQ9H668.
PaxDbiQ9H668.
PeptideAtlasiQ9H668.
PRIDEiQ9H668.

PTM databases

iPTMnetiQ9H668.
PhosphoSiteiQ9H668.

Expressioni

Gene expression databases

BgeeiENSG00000107960.
CleanExiHS_OBFC1.
GenevisibleiQ9H668. HS.

Organism-specific databases

HPAiHPA037924.
HPA037925.

Interactioni

Subunit structurei

Component of the CST complex, composed of TEN1/C17orf106, CTC1/C17orf68 and STN1/OBFC1; in the complex interacts directly with TEN1 and CTC1. Interacts with ACD/TPP1, POT1 and POLA1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACDQ96AP04EBI-746930,EBI-717666
CTC1Q2NKJ36EBI-746930,EBI-2562802
LDLRAP1Q5SW963EBI-746930,EBI-747813
TEN1Q86WV53EBI-746930,EBI-2562799

Protein-protein interaction databases

BioGridi123054. 58 interactions.
DIPiDIP-59914N.
IntActiQ9H668. 16 interactions.
MINTiMINT-1482105.
STRINGi9606.ENSP00000224950.

Structurei

Secondary structure

1
368
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 366Combined sources
Beta strandi41 – 433Combined sources
Beta strandi47 – 493Combined sources
Beta strandi52 – 543Combined sources
Beta strandi56 – 6813Combined sources
Beta strandi70 – 789Combined sources
Beta strandi83 – 897Combined sources
Helixi113 – 1175Combined sources
Helixi118 – 1225Combined sources
Beta strandi131 – 14111Combined sources
Beta strandi144 – 15512Combined sources
Helixi161 – 17616Combined sources
Turni177 – 1793Combined sources
Helixi191 – 1944Combined sources
Helixi196 – 1983Combined sources
Helixi201 – 21818Combined sources
Beta strandi223 – 2253Combined sources
Helixi226 – 2305Combined sources
Helixi233 – 2397Combined sources
Helixi242 – 2454Combined sources
Helixi259 – 27618Combined sources
Beta strandi279 – 2813Combined sources
Beta strandi284 – 2863Combined sources
Beta strandi290 – 2934Combined sources
Helixi294 – 2963Combined sources
Helixi298 – 30912Combined sources
Helixi311 – 3133Combined sources
Beta strandi320 – 3234Combined sources
Helixi324 – 33411Combined sources
Helixi341 – 35313Combined sources
Beta strandi356 – 3616Combined sources
Beta strandi364 – 3674Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JOIX-ray2.05A/B19-184[»]
4JQFX-ray1.60A191-368[»]
ProteinModelPortaliQ9H668.
SMRiQ9H668. Positions 24-183, 191-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 185185Interaction with CTC11 PublicationAdd
BLAST
Regioni191 – 295105Winged helix-turn-helix (wHTH) 1Add
BLAST
Regioni296 – 36873Winged helix-turn-helix (wHTH) 2Add
BLAST

Sequence similaritiesi

Belongs to the STN1 family.Curated
Contains 1 OB DNA-binding domain.Curated

Phylogenomic databases

eggNOGiKOG3108. Eukaryota.
COG5235. LUCA.
GeneTreeiENSGT00390000000909.
HOGENOMiHOG000088638.
HOVERGENiHBG053364.
InParanoidiQ9H668.
OMAiQTFYQQE.
OrthoDBiEOG091G0A0K.
PhylomeDBiQ9H668.
TreeFamiTF328623.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR015253. CST_STN1_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR014647. Stn1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13989:SF14. PTHR13989:SF14. 1 hit.
PfamiPF09170. STN1_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF036950. UCP036950. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50249. SSF50249. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9H668-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPGSSRCEE ETPSLLWGLD PVFLAFAKLY IRDILDMKES RQVPGVFLYN
60 70 80 90 100
GHPIKQVDVL GTVIGVRERD AFYSYGVDDS TGVINCICWK KLNTESVSAA
110 120 130 140 150
PSAARELSLT SQLKKLQETI EQKTKIEIGD TIRVRGSIRT YREEREIHAT
160 170 180 190 200
TYYKVDDPVW NIQIARMLEL PTIYRKVYDQ PFHSSALEKE EALSNPGALD
210 220 230 240 250
LPSLTSLLSE KAKEFLMENR VQSFYQQELE MVESLLSLAN QPVIHSASSD
260 270 280 290 300
QVNFKKDTTS KAIHSIFKNA IQLLQEKGLV FQKDDGFDNL YYVTREDKDL
310 320 330 340 350
HRKIHRIIQQ DCQKPNHMEK GCHFLHILAC ARLSIRPGLS EAVLQQVLEL
360
LEDQSDIVST MEHYYTAF
Length:368
Mass (Da):42,119
Last modified:April 26, 2005 - v2
Checksum:i087E7EF75544A1F0
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti151 – 1511T → A.2 Publications
Corresponds to variant rs2487999 [ dbSNP | Ensembl ].
VAR_022364
Natural varianti248 – 2481S → C.2 Publications
Corresponds to variant rs10786775 [ dbSNP | Ensembl ].
VAR_022365

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026212 mRNA. Translation: BAB15396.1.
AL133355 Genomic DNA. Translation: CAB81623.1.
CH471066 Genomic DNA. Translation: EAW49619.1.
CH471066 Genomic DNA. Translation: EAW49620.1.
BC017400 mRNA. Translation: AAH17400.1.
CCDSiCCDS7552.1.
RefSeqiNP_079204.2. NM_024928.4.
XP_006718039.1. XM_006717976.3.
UniGeneiHs.62314.

Genome annotation databases

EnsembliENST00000224950; ENSP00000224950; ENSG00000107960.
ENST00000369764; ENSP00000358779; ENSG00000107960.
GeneIDi79991.
KEGGihsa:79991.
UCSCiuc001kxl.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026212 mRNA. Translation: BAB15396.1.
AL133355 Genomic DNA. Translation: CAB81623.1.
CH471066 Genomic DNA. Translation: EAW49619.1.
CH471066 Genomic DNA. Translation: EAW49620.1.
BC017400 mRNA. Translation: AAH17400.1.
CCDSiCCDS7552.1.
RefSeqiNP_079204.2. NM_024928.4.
XP_006718039.1. XM_006717976.3.
UniGeneiHs.62314.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JOIX-ray2.05A/B19-184[»]
4JQFX-ray1.60A191-368[»]
ProteinModelPortaliQ9H668.
SMRiQ9H668. Positions 24-183, 191-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123054. 58 interactions.
DIPiDIP-59914N.
IntActiQ9H668. 16 interactions.
MINTiMINT-1482105.
STRINGi9606.ENSP00000224950.

PTM databases

iPTMnetiQ9H668.
PhosphoSiteiQ9H668.

Polymorphism and mutation databases

BioMutaiOBFC1.
DMDMi62900737.

Proteomic databases

EPDiQ9H668.
MaxQBiQ9H668.
PaxDbiQ9H668.
PeptideAtlasiQ9H668.
PRIDEiQ9H668.

Protocols and materials databases

DNASUi79991.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000224950; ENSP00000224950; ENSG00000107960.
ENST00000369764; ENSP00000358779; ENSG00000107960.
GeneIDi79991.
KEGGihsa:79991.
UCSCiuc001kxl.4. human.

Organism-specific databases

CTDi79991.
GeneCardsiOBFC1.
HGNCiHGNC:26200. OBFC1.
HPAiHPA037924.
HPA037925.
MalaCardsiOBFC1.
MIMi613128. gene.
neXtProtiNX_Q9H668.
Orphaneti2032. Idiopathic pulmonary fibrosis.
PharmGKBiPA134987118.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3108. Eukaryota.
COG5235. LUCA.
GeneTreeiENSGT00390000000909.
HOGENOMiHOG000088638.
HOVERGENiHBG053364.
InParanoidiQ9H668.
OMAiQTFYQQE.
OrthoDBiEOG091G0A0K.
PhylomeDBiQ9H668.
TreeFamiTF328623.

Miscellaneous databases

ChiTaRSiOBFC1. human.
GenomeRNAii79991.
PROiQ9H668.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107960.
CleanExiHS_OBFC1.
GenevisibleiQ9H668. HS.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR015253. CST_STN1_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR014647. Stn1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13989:SF14. PTHR13989:SF14. 1 hit.
PfamiPF09170. STN1_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF036950. UCP036950. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50249. SSF50249. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTN1_HUMAN
AccessioniPrimary (citable) accession number: Q9H668
Secondary accession number(s): D3DR99, Q5TCZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: September 7, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Cells expressing STN1 mutants defective for dimerization with TEN1 display elongated telomeres and telomere defects associated with telomere uncapping.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.