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Protein

Probable ATP-dependent RNA helicase DHX35

Gene

DHX35

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in pre-mRNA splicing.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: UniProtKB
  • RNA processing Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DHX35 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 35
Gene namesi
Name:DHX35
Synonyms:C20orf15, DDX35
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101452.14
HGNCiHGNC:15861 DHX35
neXtProtiNX_Q9H5Z1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi60625
OpenTargetsiENSG00000101452
PharmGKBiPA27222

Polymorphism and mutation databases

BioMutaiDHX35

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551681 – 703Probable ATP-dependent RNA helicase DHX35Add BLAST703

Proteomic databases

EPDiQ9H5Z1
MaxQBiQ9H5Z1
PaxDbiQ9H5Z1
PeptideAtlasiQ9H5Z1
PRIDEiQ9H5Z1

PTM databases

iPTMnetiQ9H5Z1
PhosphoSitePlusiQ9H5Z1

Expressioni

Gene expression databases

BgeeiENSG00000101452
CleanExiHS_DHX35
ExpressionAtlasiQ9H5Z1 baseline and differential
GenevisibleiQ9H5Z1 HS

Organism-specific databases

HPAiHPA054451
HPA062700

Interactioni

Subunit structurei

Identified in the spliceosome C complex.1 Publication

Protein-protein interaction databases

BioGridi121945, 32 interactors
CORUMiQ9H5Z1
IntActiQ9H5Z1, 12 interactors
STRINGi9606.ENSP00000252011

Structurei

3D structure databases

ProteinModelPortaliQ9H5Z1
SMRiQ9H5Z1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 229Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini261 – 438Helicase C-terminalPROSITE-ProRule annotationAdd BLAST178

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi176 – 179DEAH box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0925 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
HOVERGENiHBG039428
InParanoidiQ9H5Z1
KOiK13117
OMAiQSPVPDY
OrthoDBiEOG091G0T3A
PhylomeDBiQ9H5Z1
TreeFamiTF105843

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H5Z1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPVGPVKF WRPGTEGPGV SISEERQSLA ENSGTTVVYN PYAALSIEQQ
60 70 80 90 100
RQKLPVFKLR NHILYLIENY QTVVIVGETG CGKSTQIPQY LAEAGWTAEG
110 120 130 140 150
RVVGVTQPRR VAAVTVAGRV AEERGAVLGH EVGYCIRFDD CTDQLATRIK
160 170 180 190 200
FLTDGMLVRE MMVDPLLTKY SVIMLDEAHE RTLYTDIAIG LLKKIQKKRG
210 220 230 240 250
DLRLIVASAT LDADKFRDFF NQNETSDPAR DTCVILTVEG RTFPVDIFYL
260 270 280 290 300
QSPVPDYIKS TVETVVKIHQ TEGDGDVLAF LTGQEEVETV VSMLIEQARA
310 320 330 340 350
LARTGMKRHL RVLPMYAGLP SFEQMKVFER VSRSVRKVIV ATNVAETSIT
360 370 380 390 400
ISGIVYVIDC GFVKLRAYNP RTAIECLVVV PVSQASANQR AGRGGRSRSG
410 420 430 440 450
KCYRLYTEEA FDKLPQSTVP EMQRSNLAPV ILQLKALGID NVLRFHFMSP
460 470 480 490 500
PPAQSMVQAL ELLYALGGLD KDCRLTEPLG MRIAEFPLNP MFAKMLLESG
510 520 530 540 550
NFGCSQEILS IAAMMQIQNI FVVPPNQKSH AIRVHRKFAV EEGDHLTMLN
560 570 580 590 600
IYEAFIKHNK DSKWCQEHFL NYKGLVRAAT VREQLKKLLV KFQVPRKSSE
610 620 630 640 650
GDPDLVLRCI VSGFFANAAR FHSTGAYRTI RDDHELHIHP ASVLYAEKPP
660 670 680 690 700
RWVIYNEVIQ TSKYYMRDVT AIESAWLLEL APHFYQQGTH LSLKAKRAKV

QDP
Length:703
Mass (Da):78,910
Last modified:January 23, 2002 - v2
Checksum:iCFC03B2A90CE5819
GO
Isoform 2 (identifier: Q9H5Z1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-89: Missing.

Note: No experimental confirmation available.
Show »
Length:672
Mass (Da):75,440
Checksum:iF771E3502CEDC6F7
GO

Sequence cautioni

The sequence BAB15166 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35T → I in BAG64452 (PubMed:14702039).Curated1
Sequence conflicti582R → G in BAB15476 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052184189I → T. Corresponds to variant dbSNP:rs36053162Ensembl.1
Natural variantiVAR_020211703P → L. Corresponds to variant dbSNP:rs3752302Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04717859 – 89Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026412 mRNA Translation: BAB15476.1
AK025541 mRNA Translation: BAB15166.1 Different initiation.
AK303396 mRNA Translation: BAG64452.1
AL023803 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76006.1
BC132669 mRNA Translation: AAI32670.1
CCDSiCCDS13310.1 [Q9H5Z1-1]
CCDS54463.1 [Q9H5Z1-2]
RefSeqiNP_001177738.1, NM_001190809.1 [Q9H5Z1-2]
NP_068750.2, NM_021931.3 [Q9H5Z1-1]
UniGeneiHs.444520

Genome annotation databases

EnsembliENST00000252011; ENSP00000252011; ENSG00000101452 [Q9H5Z1-1]
ENST00000373323; ENSP00000362420; ENSG00000101452 [Q9H5Z1-2]
GeneIDi60625
KEGGihsa:60625
UCSCiuc002xjh.4 human [Q9H5Z1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDHX35_HUMAN
AccessioniPrimary (citable) accession number: Q9H5Z1
Secondary accession number(s): A2RTX3
, B4E0J0, F5GXM6, Q5THR0, Q9H4H7, Q9H6T6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: May 23, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

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