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Q9H5Z1

- DHX35_HUMAN

UniProt

Q9H5Z1 - DHX35_HUMAN

Protein

Probable ATP-dependent RNA helicase DHX35

Gene

DHX35

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    May be involved in pre-mRNA splicing.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi77 – 848ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: UniProtKB

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent RNA helicase DHX35 (EC:3.6.4.13)
    Alternative name(s):
    DEAH box protein 35
    Gene namesi
    Name:DHX35
    Synonyms:C20orf15, DDX35
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:15861. DHX35.

    Subcellular locationi

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: UniProtKB

    Keywords - Cellular componenti

    Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27222.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 703703Probable ATP-dependent RNA helicase DHX35PRO_0000055168Add
    BLAST

    Proteomic databases

    MaxQBiQ9H5Z1.
    PaxDbiQ9H5Z1.
    PRIDEiQ9H5Z1.

    PTM databases

    PhosphoSiteiQ9H5Z1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9H5Z1.
    BgeeiQ9H5Z1.
    CleanExiHS_DHX35.
    GenevestigatoriQ9H5Z1.

    Organism-specific databases

    HPAiHPA054451.

    Interactioni

    Subunit structurei

    Identified in the spliceosome C complex.1 Publication

    Protein-protein interaction databases

    BioGridi121945. 2 interactions.
    IntActiQ9H5Z1. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H5Z1.
    SMRiQ9H5Z1. Positions 23-700.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini64 – 229166Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini261 – 438178Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi176 – 1794DEAH box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1643.
    HOGENOMiHOG000175261.
    HOVERGENiHBG039428.
    InParanoidiQ9H5Z1.
    KOiK13117.
    OMAiMAEFPLN.
    PhylomeDBiQ9H5Z1.
    TreeFamiTF105843.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9H5Z1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAPVGPVKF WRPGTEGPGV SISEERQSLA ENSGTTVVYN PYAALSIEQQ    50
    RQKLPVFKLR NHILYLIENY QTVVIVGETG CGKSTQIPQY LAEAGWTAEG 100
    RVVGVTQPRR VAAVTVAGRV AEERGAVLGH EVGYCIRFDD CTDQLATRIK 150
    FLTDGMLVRE MMVDPLLTKY SVIMLDEAHE RTLYTDIAIG LLKKIQKKRG 200
    DLRLIVASAT LDADKFRDFF NQNETSDPAR DTCVILTVEG RTFPVDIFYL 250
    QSPVPDYIKS TVETVVKIHQ TEGDGDVLAF LTGQEEVETV VSMLIEQARA 300
    LARTGMKRHL RVLPMYAGLP SFEQMKVFER VSRSVRKVIV ATNVAETSIT 350
    ISGIVYVIDC GFVKLRAYNP RTAIECLVVV PVSQASANQR AGRGGRSRSG 400
    KCYRLYTEEA FDKLPQSTVP EMQRSNLAPV ILQLKALGID NVLRFHFMSP 450
    PPAQSMVQAL ELLYALGGLD KDCRLTEPLG MRIAEFPLNP MFAKMLLESG 500
    NFGCSQEILS IAAMMQIQNI FVVPPNQKSH AIRVHRKFAV EEGDHLTMLN 550
    IYEAFIKHNK DSKWCQEHFL NYKGLVRAAT VREQLKKLLV KFQVPRKSSE 600
    GDPDLVLRCI VSGFFANAAR FHSTGAYRTI RDDHELHIHP ASVLYAEKPP 650
    RWVIYNEVIQ TSKYYMRDVT AIESAWLLEL APHFYQQGTH LSLKAKRAKV 700
    QDP 703
    Length:703
    Mass (Da):78,910
    Last modified:January 23, 2002 - v2
    Checksum:iCFC03B2A90CE5819
    GO
    Isoform 2 (identifier: Q9H5Z1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         59-89: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:672
    Mass (Da):75,440
    Checksum:iF771E3502CEDC6F7
    GO

    Sequence cautioni

    The sequence BAB15166.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351T → I in BAG64452. (PubMed:14702039)Curated
    Sequence conflicti582 – 5821R → G in BAB15476. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti189 – 1891I → T.
    Corresponds to variant rs36053162 [ dbSNP | Ensembl ].
    VAR_052184
    Natural varianti703 – 7031P → L.
    Corresponds to variant rs3752302 [ dbSNP | Ensembl ].
    VAR_020211

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei59 – 8931Missing in isoform 2. 1 PublicationVSP_047178Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK026412 mRNA. Translation: BAB15476.1.
    AK025541 mRNA. Translation: BAB15166.1. Different initiation.
    AK303396 mRNA. Translation: BAG64452.1.
    AL023803 Genomic DNA. Translation: CAI22035.1.
    CH471077 Genomic DNA. Translation: EAW76006.1.
    BC132669 mRNA. Translation: AAI32670.1.
    CCDSiCCDS13310.1. [Q9H5Z1-1]
    CCDS54463.1. [Q9H5Z1-2]
    RefSeqiNP_001177738.1. NM_001190809.1. [Q9H5Z1-2]
    NP_068750.2. NM_021931.3. [Q9H5Z1-1]
    UniGeneiHs.444520.

    Genome annotation databases

    EnsembliENST00000252011; ENSP00000252011; ENSG00000101452. [Q9H5Z1-1]
    ENST00000373323; ENSP00000362420; ENSG00000101452. [Q9H5Z1-2]
    GeneIDi60625.
    KEGGihsa:60625.
    UCSCiuc002xjh.3. human. [Q9H5Z1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK026412 mRNA. Translation: BAB15476.1 .
    AK025541 mRNA. Translation: BAB15166.1 . Different initiation.
    AK303396 mRNA. Translation: BAG64452.1 .
    AL023803 Genomic DNA. Translation: CAI22035.1 .
    CH471077 Genomic DNA. Translation: EAW76006.1 .
    BC132669 mRNA. Translation: AAI32670.1 .
    CCDSi CCDS13310.1. [Q9H5Z1-1 ]
    CCDS54463.1. [Q9H5Z1-2 ]
    RefSeqi NP_001177738.1. NM_001190809.1. [Q9H5Z1-2 ]
    NP_068750.2. NM_021931.3. [Q9H5Z1-1 ]
    UniGenei Hs.444520.

    3D structure databases

    ProteinModelPortali Q9H5Z1.
    SMRi Q9H5Z1. Positions 23-700.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121945. 2 interactions.
    IntActi Q9H5Z1. 1 interaction.

    PTM databases

    PhosphoSitei Q9H5Z1.

    Proteomic databases

    MaxQBi Q9H5Z1.
    PaxDbi Q9H5Z1.
    PRIDEi Q9H5Z1.

    Protocols and materials databases

    DNASUi 60625.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000252011 ; ENSP00000252011 ; ENSG00000101452 . [Q9H5Z1-1 ]
    ENST00000373323 ; ENSP00000362420 ; ENSG00000101452 . [Q9H5Z1-2 ]
    GeneIDi 60625.
    KEGGi hsa:60625.
    UCSCi uc002xjh.3. human. [Q9H5Z1-1 ]

    Organism-specific databases

    CTDi 60625.
    GeneCardsi GC20P037590.
    HGNCi HGNC:15861. DHX35.
    HPAi HPA054451.
    neXtProti NX_Q9H5Z1.
    PharmGKBi PA27222.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1643.
    HOGENOMi HOG000175261.
    HOVERGENi HBG039428.
    InParanoidi Q9H5Z1.
    KOi K13117.
    OMAi MAEFPLN.
    PhylomeDBi Q9H5Z1.
    TreeFami TF105843.

    Miscellaneous databases

    GenomeRNAii 60625.
    NextBioi 35476770.
    PROi Q9H5Z1.

    Gene expression databases

    ArrayExpressi Q9H5Z1.
    Bgeei Q9H5Z1.
    CleanExi HS_DHX35.
    Genevestigatori Q9H5Z1.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Ileal mucosa and Thymus.
    2. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    5. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
      Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
      RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.

    Entry informationi

    Entry nameiDHX35_HUMAN
    AccessioniPrimary (citable) accession number: Q9H5Z1
    Secondary accession number(s): A2RTX3
    , B4E0J0, F5GXM6, Q5THR0, Q9H4H7, Q9H6T6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3