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Protein

Probable ATP-dependent RNA helicase DHX35

Gene

DHX35

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in pre-mRNA splicing.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: UniProtKB
  • RNA processing Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101452-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DHX35 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 35
Gene namesi
Name:DHX35
Synonyms:C20orf15, DDX35
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:15861. DHX35.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • cytoplasm Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi60625.
OpenTargetsiENSG00000101452.
PharmGKBiPA27222.

Polymorphism and mutation databases

BioMutaiDHX35.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551681 – 703Probable ATP-dependent RNA helicase DHX35Add BLAST703

Proteomic databases

EPDiQ9H5Z1.
MaxQBiQ9H5Z1.
PaxDbiQ9H5Z1.
PeptideAtlasiQ9H5Z1.
PRIDEiQ9H5Z1.

PTM databases

iPTMnetiQ9H5Z1.
PhosphoSitePlusiQ9H5Z1.

Expressioni

Gene expression databases

BgeeiENSG00000101452.
CleanExiHS_DHX35.
ExpressionAtlasiQ9H5Z1. baseline and differential.
GenevisibleiQ9H5Z1. HS.

Organism-specific databases

HPAiHPA054451.
HPA062700.

Interactioni

Subunit structurei

Identified in the spliceosome C complex.1 Publication

Protein-protein interaction databases

BioGridi121945. 27 interactors.
IntActiQ9H5Z1. 10 interactors.
STRINGi9606.ENSP00000252011.

Structurei

3D structure databases

ProteinModelPortaliQ9H5Z1.
SMRiQ9H5Z1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 229Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini261 – 438Helicase C-terminalPROSITE-ProRule annotationAdd BLAST178

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi176 – 179DEAH box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ9H5Z1.
KOiK13117.
OMAiYQTLVIV.
OrthoDBiEOG091G0T3A.
PhylomeDBiQ9H5Z1.
TreeFamiTF105843.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H5Z1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPVGPVKF WRPGTEGPGV SISEERQSLA ENSGTTVVYN PYAALSIEQQ
60 70 80 90 100
RQKLPVFKLR NHILYLIENY QTVVIVGETG CGKSTQIPQY LAEAGWTAEG
110 120 130 140 150
RVVGVTQPRR VAAVTVAGRV AEERGAVLGH EVGYCIRFDD CTDQLATRIK
160 170 180 190 200
FLTDGMLVRE MMVDPLLTKY SVIMLDEAHE RTLYTDIAIG LLKKIQKKRG
210 220 230 240 250
DLRLIVASAT LDADKFRDFF NQNETSDPAR DTCVILTVEG RTFPVDIFYL
260 270 280 290 300
QSPVPDYIKS TVETVVKIHQ TEGDGDVLAF LTGQEEVETV VSMLIEQARA
310 320 330 340 350
LARTGMKRHL RVLPMYAGLP SFEQMKVFER VSRSVRKVIV ATNVAETSIT
360 370 380 390 400
ISGIVYVIDC GFVKLRAYNP RTAIECLVVV PVSQASANQR AGRGGRSRSG
410 420 430 440 450
KCYRLYTEEA FDKLPQSTVP EMQRSNLAPV ILQLKALGID NVLRFHFMSP
460 470 480 490 500
PPAQSMVQAL ELLYALGGLD KDCRLTEPLG MRIAEFPLNP MFAKMLLESG
510 520 530 540 550
NFGCSQEILS IAAMMQIQNI FVVPPNQKSH AIRVHRKFAV EEGDHLTMLN
560 570 580 590 600
IYEAFIKHNK DSKWCQEHFL NYKGLVRAAT VREQLKKLLV KFQVPRKSSE
610 620 630 640 650
GDPDLVLRCI VSGFFANAAR FHSTGAYRTI RDDHELHIHP ASVLYAEKPP
660 670 680 690 700
RWVIYNEVIQ TSKYYMRDVT AIESAWLLEL APHFYQQGTH LSLKAKRAKV

QDP
Length:703
Mass (Da):78,910
Last modified:January 23, 2002 - v2
Checksum:iCFC03B2A90CE5819
GO
Isoform 2 (identifier: Q9H5Z1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-89: Missing.

Note: No experimental confirmation available.
Show »
Length:672
Mass (Da):75,440
Checksum:iF771E3502CEDC6F7
GO

Sequence cautioni

The sequence BAB15166 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35T → I in BAG64452 (PubMed:14702039).Curated1
Sequence conflicti582R → G in BAB15476 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052184189I → T.Corresponds to variant rs36053162dbSNPEnsembl.1
Natural variantiVAR_020211703P → L.Corresponds to variant rs3752302dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04717859 – 89Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026412 mRNA. Translation: BAB15476.1.
AK025541 mRNA. Translation: BAB15166.1. Different initiation.
AK303396 mRNA. Translation: BAG64452.1.
AL023803 Genomic DNA. Translation: CAI22035.1.
CH471077 Genomic DNA. Translation: EAW76006.1.
BC132669 mRNA. Translation: AAI32670.1.
CCDSiCCDS13310.1. [Q9H5Z1-1]
CCDS54463.1. [Q9H5Z1-2]
RefSeqiNP_001177738.1. NM_001190809.1. [Q9H5Z1-2]
NP_068750.2. NM_021931.3. [Q9H5Z1-1]
UniGeneiHs.444520.

Genome annotation databases

EnsembliENST00000252011; ENSP00000252011; ENSG00000101452. [Q9H5Z1-1]
ENST00000373323; ENSP00000362420; ENSG00000101452. [Q9H5Z1-2]
GeneIDi60625.
KEGGihsa:60625.
UCSCiuc002xjh.4. human. [Q9H5Z1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026412 mRNA. Translation: BAB15476.1.
AK025541 mRNA. Translation: BAB15166.1. Different initiation.
AK303396 mRNA. Translation: BAG64452.1.
AL023803 Genomic DNA. Translation: CAI22035.1.
CH471077 Genomic DNA. Translation: EAW76006.1.
BC132669 mRNA. Translation: AAI32670.1.
CCDSiCCDS13310.1. [Q9H5Z1-1]
CCDS54463.1. [Q9H5Z1-2]
RefSeqiNP_001177738.1. NM_001190809.1. [Q9H5Z1-2]
NP_068750.2. NM_021931.3. [Q9H5Z1-1]
UniGeneiHs.444520.

3D structure databases

ProteinModelPortaliQ9H5Z1.
SMRiQ9H5Z1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121945. 27 interactors.
IntActiQ9H5Z1. 10 interactors.
STRINGi9606.ENSP00000252011.

PTM databases

iPTMnetiQ9H5Z1.
PhosphoSitePlusiQ9H5Z1.

Polymorphism and mutation databases

BioMutaiDHX35.

Proteomic databases

EPDiQ9H5Z1.
MaxQBiQ9H5Z1.
PaxDbiQ9H5Z1.
PeptideAtlasiQ9H5Z1.
PRIDEiQ9H5Z1.

Protocols and materials databases

DNASUi60625.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252011; ENSP00000252011; ENSG00000101452. [Q9H5Z1-1]
ENST00000373323; ENSP00000362420; ENSG00000101452. [Q9H5Z1-2]
GeneIDi60625.
KEGGihsa:60625.
UCSCiuc002xjh.4. human. [Q9H5Z1-1]

Organism-specific databases

CTDi60625.
DisGeNETi60625.
GeneCardsiDHX35.
HGNCiHGNC:15861. DHX35.
HPAiHPA054451.
HPA062700.
neXtProtiNX_Q9H5Z1.
OpenTargetsiENSG00000101452.
PharmGKBiPA27222.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ9H5Z1.
KOiK13117.
OMAiYQTLVIV.
OrthoDBiEOG091G0T3A.
PhylomeDBiQ9H5Z1.
TreeFamiTF105843.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101452-MONOMER.

Miscellaneous databases

ChiTaRSiDHX35. human.
GenomeRNAii60625.
PROiQ9H5Z1.

Gene expression databases

BgeeiENSG00000101452.
CleanExiHS_DHX35.
ExpressionAtlasiQ9H5Z1. baseline and differential.
GenevisibleiQ9H5Z1. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHX35_HUMAN
AccessioniPrimary (citable) accession number: Q9H5Z1
Secondary accession number(s): A2RTX3
, B4E0J0, F5GXM6, Q5THR0, Q9H4H7, Q9H6T6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.