Q9H5Z1 (DHX35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ATP-dependent RNA helicase DHX35
EC=3.6.4.13

Alternative name(s):
DEAH box protein 35

Gene names

Name:	DHX35
Synonyms:	C20orf15, DDX35

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in pre-mRNA splicing.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Identified in the spliceosome C complex. Ref.5
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence BAB15166.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Spliceosome
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred by curator Ref.5. Source: UniProtKB
Cellular_component	catalytic step 2 spliceosome Inferred from direct assay Ref.5. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 703	703	Probable ATP-dependent RNA helicase DHX35		PRO_0000055168
Regions
	Domain	64 – 229	166	Helicase ATP-binding
	Domain	261 – 438	178	Helicase C-terminal
	Nucleotide binding	77 – 84	8	ATP By similarity
	Motif	176 – 179	4	DEAH box
Natural variations
	Natural variant	189	1	I → T. Corresponds to variant rs36053162 [ dbSNP | Ensembl ].		VAR_052184
	Natural variant	703	1	P → L. Corresponds to variant rs3752302 [ dbSNP | Ensembl ].		VAR_020211
Experimental info
	Sequence conflict	582	1	R → G in BAB15476. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9H5Z1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: CFC03B2A90CE5819	FASTA	703	78,910
	10 20 30 40 50 60 MAAPVGPVKF WRPGTEGPGV SISEERQSLA ENSGTTVVYN PYAALSIEQQ RQKLPVFKLR 70 80 90 100 110 120 NHILYLIENY QTVVIVGETG CGKSTQIPQY LAEAGWTAEG RVVGVTQPRR VAAVTVAGRV 130 140 150 160 170 180 AEERGAVLGH EVGYCIRFDD CTDQLATRIK FLTDGMLVRE MMVDPLLTKY SVIMLDEAHE 190 200 210 220 230 240 RTLYTDIAIG LLKKIQKKRG DLRLIVASAT LDADKFRDFF NQNETSDPAR DTCVILTVEG 250 260 270 280 290 300 RTFPVDIFYL QSPVPDYIKS TVETVVKIHQ TEGDGDVLAF LTGQEEVETV VSMLIEQARA 310 320 330 340 350 360 LARTGMKRHL RVLPMYAGLP SFEQMKVFER VSRSVRKVIV ATNVAETSIT ISGIVYVIDC 370 380 390 400 410 420 GFVKLRAYNP RTAIECLVVV PVSQASANQR AGRGGRSRSG KCYRLYTEEA FDKLPQSTVP 430 440 450 460 470 480 EMQRSNLAPV ILQLKALGID NVLRFHFMSP PPAQSMVQAL ELLYALGGLD KDCRLTEPLG 490 500 510 520 530 540 MRIAEFPLNP MFAKMLLESG NFGCSQEILS IAAMMQIQNI FVVPPNQKSH AIRVHRKFAV 550 560 570 580 590 600 EEGDHLTMLN IYEAFIKHNK DSKWCQEHFL NYKGLVRAAT VREQLKKLLV KFQVPRKSSE 610 620 630 640 650 660 GDPDLVLRCI VSGFFANAAR FHSTGAYRTI RDDHELHIHP ASVLYAEKPP RWVIYNEVIQ 670 680 690 700 TSKYYMRDVT AIESAWLLEL APHFYQQGTH LSLKAKRAKV QDP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ileal mucosa.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK026412 mRNA. Translation: BAB15476.1. AK025541 mRNA. Translation: BAB15166.1. Different initiation. AL023803 Genomic DNA. Translation: CAI22035.1. CH471077 Genomic DNA. Translation: EAW76006.1. BC132669 mRNA. Translation: AAI32670.1.
IPI	IPI00030288.
RefSeq	NP_068750.2. NM_021931.3.
UniGene	Hs.444520.
3D structure databases
ProteinModelPortal	Q9H5Z1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H5Z1. 1 interaction.
PTM databases
PhosphoSite	Q9H5Z1.
Polymorphism databases
DMDM	20137971.
Proteomic databases
PaxDb	Q9H5Z1.
PRIDE	Q9H5Z1.
Protocols and materials databases
DNASU	60625.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000252011; ENSP00000252011; ENSG00000101452.
GeneID	60625.
KEGG	hsa:60625.
UCSC	uc002xjh.3. human.
Organism-specific databases
CTD	60625.
GeneCards	GC20P037590.
HGNC	HGNC:15861. DHX35.
HPA	HPA054451.
neXtProt	NX_Q9H5Z1.
PharmGKB	PA27222.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1643.
HOGENOM	HOG000175261.
HOVERGEN	HBG039428.
InParanoid	Q9H5Z1.
KO	K13117.
OMA	NIFVVPP.
PhylomeDB	Q9H5Z1.
Gene expression databases
ArrayExpress	Q9H5Z1.
Bgee	Q9H5Z1.
CleanEx	HS_DHX35.
Genevestigator	Q9H5Z1.
GermOnline	ENSG00000101452. Homo sapiens.
Family and domain databases
InterPro	IPR002464. DNA/RNA_helicase_DEAH_CS. IPR011709. DUF1605. IPR007502. Helicase-assoc_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF04408. HA2. 1 hit. PF00271. Helicase_C. 1 hit. PF07717. OB_NTP_bind. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00847. HA2. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	60625.
NextBio	65461.

Entry information

Entry name

DHX35_HUMAN

Accession

Primary (citable) accession number: Q9H5Z1
Secondary accession number(s): A2RTX3 , Q5THR0, Q9H4H7, Q9H6T6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2002
Last modified:	May 29, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.