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Q9H5Z1 (DHX35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent RNA helicase DHX35

EC=3.6.4.13
Alternative name(s):
DEAH box protein 35
Gene names
Name:DHX35
Synonyms:C20orf15, DDX35
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length703 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in pre-mRNA splicing.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Identified in the spliceosome C complex. Ref.5

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence caution

The sequence BAB15166.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentSpliceosome
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandATP-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA splicing, via spliceosome

Inferred by curator Ref.5. Source: UniProtKB

   Cellular_componentcatalytic step 2 spliceosome

Inferred from direct assay Ref.5. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H5Z1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H5Z1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     59-89: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 703703Probable ATP-dependent RNA helicase DHX35
PRO_0000055168

Regions

Domain64 – 229166Helicase ATP-binding
Domain261 – 438178Helicase C-terminal
Nucleotide binding77 – 848ATP By similarity
Motif176 – 1794DEAH box

Natural variations

Alternative sequence59 – 8931Missing in isoform 2.
VSP_047178
Natural variant1891I → T.
Corresponds to variant rs36053162 [ dbSNP | Ensembl ].
VAR_052184
Natural variant7031P → L.
Corresponds to variant rs3752302 [ dbSNP | Ensembl ].
VAR_020211

Experimental info

Sequence conflict351T → I in BAG64452. Ref.1
Sequence conflict5821R → G in BAB15476. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: CFC03B2A90CE5819

FASTA70378,910
        10         20         30         40         50         60 
MAAPVGPVKF WRPGTEGPGV SISEERQSLA ENSGTTVVYN PYAALSIEQQ RQKLPVFKLR 

        70         80         90        100        110        120 
NHILYLIENY QTVVIVGETG CGKSTQIPQY LAEAGWTAEG RVVGVTQPRR VAAVTVAGRV 

       130        140        150        160        170        180 
AEERGAVLGH EVGYCIRFDD CTDQLATRIK FLTDGMLVRE MMVDPLLTKY SVIMLDEAHE 

       190        200        210        220        230        240 
RTLYTDIAIG LLKKIQKKRG DLRLIVASAT LDADKFRDFF NQNETSDPAR DTCVILTVEG 

       250        260        270        280        290        300 
RTFPVDIFYL QSPVPDYIKS TVETVVKIHQ TEGDGDVLAF LTGQEEVETV VSMLIEQARA 

       310        320        330        340        350        360 
LARTGMKRHL RVLPMYAGLP SFEQMKVFER VSRSVRKVIV ATNVAETSIT ISGIVYVIDC 

       370        380        390        400        410        420 
GFVKLRAYNP RTAIECLVVV PVSQASANQR AGRGGRSRSG KCYRLYTEEA FDKLPQSTVP 

       430        440        450        460        470        480 
EMQRSNLAPV ILQLKALGID NVLRFHFMSP PPAQSMVQAL ELLYALGGLD KDCRLTEPLG 

       490        500        510        520        530        540 
MRIAEFPLNP MFAKMLLESG NFGCSQEILS IAAMMQIQNI FVVPPNQKSH AIRVHRKFAV 

       550        560        570        580        590        600 
EEGDHLTMLN IYEAFIKHNK DSKWCQEHFL NYKGLVRAAT VREQLKKLLV KFQVPRKSSE 

       610        620        630        640        650        660 
GDPDLVLRCI VSGFFANAAR FHSTGAYRTI RDDHELHIHP ASVLYAEKPP RWVIYNEVIQ 

       670        680        690        700 
TSKYYMRDVT AIESAWLLEL APHFYQQGTH LSLKAKRAKV QDP 

« Hide

Isoform 2 [UniParc].

Checksum: F771E3502CEDC6F7
Show »

FASTA67275,440

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Ileal mucosa and Thymus.
[2]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[5]"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK026412 mRNA. Translation: BAB15476.1.
AK025541 mRNA. Translation: BAB15166.1. Different initiation.
AK303396 mRNA. Translation: BAG64452.1.
AL023803 Genomic DNA. Translation: CAI22035.1.
CH471077 Genomic DNA. Translation: EAW76006.1.
BC132669 mRNA. Translation: AAI32670.1.
CCDSCCDS13310.1. [Q9H5Z1-1]
CCDS54463.1. [Q9H5Z1-2]
RefSeqNP_001177738.1. NM_001190809.1. [Q9H5Z1-2]
NP_068750.2. NM_021931.3. [Q9H5Z1-1]
UniGeneHs.444520.

3D structure databases

ProteinModelPortalQ9H5Z1.
SMRQ9H5Z1. Positions 23-700.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121945. 2 interactions.
IntActQ9H5Z1. 1 interaction.

PTM databases

PhosphoSiteQ9H5Z1.

Proteomic databases

MaxQBQ9H5Z1.
PaxDbQ9H5Z1.
PRIDEQ9H5Z1.

Protocols and materials databases

DNASU60625.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000252011; ENSP00000252011; ENSG00000101452. [Q9H5Z1-1]
ENST00000373323; ENSP00000362420; ENSG00000101452. [Q9H5Z1-2]
GeneID60625.
KEGGhsa:60625.
UCSCuc002xjh.3. human. [Q9H5Z1-1]

Organism-specific databases

CTD60625.
GeneCardsGC20P037590.
HGNCHGNC:15861. DHX35.
HPAHPA054451.
neXtProtNX_Q9H5Z1.
PharmGKBPA27222.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1643.
HOGENOMHOG000175261.
HOVERGENHBG039428.
InParanoidQ9H5Z1.
KOK13117.
OMAMAEFPLN.
PhylomeDBQ9H5Z1.
TreeFamTF105843.

Gene expression databases

ArrayExpressQ9H5Z1.
BgeeQ9H5Z1.
CleanExHS_DHX35.
GenevestigatorQ9H5Z1.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi60625.
NextBio35476770.
PROQ9H5Z1.

Entry information

Entry nameDHX35_HUMAN
AccessionPrimary (citable) accession number: Q9H5Z1
Secondary accession number(s): A2RTX3 expand/collapse secondary AC list , B4E0J0, F5GXM6, Q5THR0, Q9H4H7, Q9H6T6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM