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Q9H5Z1

- DHX35_HUMAN

UniProt

Q9H5Z1 - DHX35_HUMAN

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Protein

Probable ATP-dependent RNA helicase DHX35

Gene

DHX35

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in pre-mRNA splicing.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 848ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DHX35 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 35
Gene namesi
Name:DHX35
Synonyms:C20orf15, DDX35
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:15861. DHX35.

Subcellular locationi

GO - Cellular componenti

  1. catalytic step 2 spliceosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27222.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 703703Probable ATP-dependent RNA helicase DHX35PRO_0000055168Add
BLAST

Proteomic databases

MaxQBiQ9H5Z1.
PaxDbiQ9H5Z1.
PRIDEiQ9H5Z1.

PTM databases

PhosphoSiteiQ9H5Z1.

Expressioni

Gene expression databases

BgeeiQ9H5Z1.
CleanExiHS_DHX35.
ExpressionAtlasiQ9H5Z1. baseline and differential.
GenevestigatoriQ9H5Z1.

Organism-specific databases

HPAiHPA054451.

Interactioni

Subunit structurei

Identified in the spliceosome C complex.1 Publication

Protein-protein interaction databases

BioGridi121945. 7 interactions.
IntActiQ9H5Z1. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9H5Z1.
SMRiQ9H5Z1. Positions 23-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 229166Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini261 – 438178Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi176 – 1794DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1643.
GeneTreeiENSGT00760000119249.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ9H5Z1.
KOiK13117.
OMAiMAEFPLN.
PhylomeDBiQ9H5Z1.
TreeFamiTF105843.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9H5Z1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPVGPVKF WRPGTEGPGV SISEERQSLA ENSGTTVVYN PYAALSIEQQ
60 70 80 90 100
RQKLPVFKLR NHILYLIENY QTVVIVGETG CGKSTQIPQY LAEAGWTAEG
110 120 130 140 150
RVVGVTQPRR VAAVTVAGRV AEERGAVLGH EVGYCIRFDD CTDQLATRIK
160 170 180 190 200
FLTDGMLVRE MMVDPLLTKY SVIMLDEAHE RTLYTDIAIG LLKKIQKKRG
210 220 230 240 250
DLRLIVASAT LDADKFRDFF NQNETSDPAR DTCVILTVEG RTFPVDIFYL
260 270 280 290 300
QSPVPDYIKS TVETVVKIHQ TEGDGDVLAF LTGQEEVETV VSMLIEQARA
310 320 330 340 350
LARTGMKRHL RVLPMYAGLP SFEQMKVFER VSRSVRKVIV ATNVAETSIT
360 370 380 390 400
ISGIVYVIDC GFVKLRAYNP RTAIECLVVV PVSQASANQR AGRGGRSRSG
410 420 430 440 450
KCYRLYTEEA FDKLPQSTVP EMQRSNLAPV ILQLKALGID NVLRFHFMSP
460 470 480 490 500
PPAQSMVQAL ELLYALGGLD KDCRLTEPLG MRIAEFPLNP MFAKMLLESG
510 520 530 540 550
NFGCSQEILS IAAMMQIQNI FVVPPNQKSH AIRVHRKFAV EEGDHLTMLN
560 570 580 590 600
IYEAFIKHNK DSKWCQEHFL NYKGLVRAAT VREQLKKLLV KFQVPRKSSE
610 620 630 640 650
GDPDLVLRCI VSGFFANAAR FHSTGAYRTI RDDHELHIHP ASVLYAEKPP
660 670 680 690 700
RWVIYNEVIQ TSKYYMRDVT AIESAWLLEL APHFYQQGTH LSLKAKRAKV

QDP
Length:703
Mass (Da):78,910
Last modified:January 23, 2002 - v2
Checksum:iCFC03B2A90CE5819
GO
Isoform 2 (identifier: Q9H5Z1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-89: Missing.

Note: No experimental confirmation available.

Show »
Length:672
Mass (Da):75,440
Checksum:iF771E3502CEDC6F7
GO

Sequence cautioni

The sequence BAB15166.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351T → I in BAG64452. (PubMed:14702039)Curated
Sequence conflicti582 – 5821R → G in BAB15476. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti189 – 1891I → T.
Corresponds to variant rs36053162 [ dbSNP | Ensembl ].
VAR_052184
Natural varianti703 – 7031P → L.
Corresponds to variant rs3752302 [ dbSNP | Ensembl ].
VAR_020211

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei59 – 8931Missing in isoform 2. 1 PublicationVSP_047178Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK026412 mRNA. Translation: BAB15476.1.
AK025541 mRNA. Translation: BAB15166.1. Different initiation.
AK303396 mRNA. Translation: BAG64452.1.
AL023803 Genomic DNA. Translation: CAI22035.1.
CH471077 Genomic DNA. Translation: EAW76006.1.
BC132669 mRNA. Translation: AAI32670.1.
CCDSiCCDS13310.1. [Q9H5Z1-1]
CCDS54463.1. [Q9H5Z1-2]
RefSeqiNP_001177738.1. NM_001190809.1. [Q9H5Z1-2]
NP_068750.2. NM_021931.3. [Q9H5Z1-1]
UniGeneiHs.444520.

Genome annotation databases

EnsembliENST00000252011; ENSP00000252011; ENSG00000101452. [Q9H5Z1-1]
ENST00000373323; ENSP00000362420; ENSG00000101452. [Q9H5Z1-2]
GeneIDi60625.
KEGGihsa:60625.
UCSCiuc002xjh.3. human. [Q9H5Z1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK026412 mRNA. Translation: BAB15476.1 .
AK025541 mRNA. Translation: BAB15166.1 . Different initiation.
AK303396 mRNA. Translation: BAG64452.1 .
AL023803 Genomic DNA. Translation: CAI22035.1 .
CH471077 Genomic DNA. Translation: EAW76006.1 .
BC132669 mRNA. Translation: AAI32670.1 .
CCDSi CCDS13310.1. [Q9H5Z1-1 ]
CCDS54463.1. [Q9H5Z1-2 ]
RefSeqi NP_001177738.1. NM_001190809.1. [Q9H5Z1-2 ]
NP_068750.2. NM_021931.3. [Q9H5Z1-1 ]
UniGenei Hs.444520.

3D structure databases

ProteinModelPortali Q9H5Z1.
SMRi Q9H5Z1. Positions 23-700.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121945. 7 interactions.
IntActi Q9H5Z1. 1 interaction.

PTM databases

PhosphoSitei Q9H5Z1.

Proteomic databases

MaxQBi Q9H5Z1.
PaxDbi Q9H5Z1.
PRIDEi Q9H5Z1.

Protocols and materials databases

DNASUi 60625.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000252011 ; ENSP00000252011 ; ENSG00000101452 . [Q9H5Z1-1 ]
ENST00000373323 ; ENSP00000362420 ; ENSG00000101452 . [Q9H5Z1-2 ]
GeneIDi 60625.
KEGGi hsa:60625.
UCSCi uc002xjh.3. human. [Q9H5Z1-1 ]

Organism-specific databases

CTDi 60625.
GeneCardsi GC20P037590.
HGNCi HGNC:15861. DHX35.
HPAi HPA054451.
neXtProti NX_Q9H5Z1.
PharmGKBi PA27222.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1643.
GeneTreei ENSGT00760000119249.
HOGENOMi HOG000175261.
HOVERGENi HBG039428.
InParanoidi Q9H5Z1.
KOi K13117.
OMAi MAEFPLN.
PhylomeDBi Q9H5Z1.
TreeFami TF105843.

Miscellaneous databases

GenomeRNAii 60625.
NextBioi 35476770.
PROi Q9H5Z1.

Gene expression databases

Bgeei Q9H5Z1.
CleanExi HS_DHX35.
ExpressionAtlasi Q9H5Z1. baseline and differential.
Genevestigatori Q9H5Z1.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Ileal mucosa and Thymus.
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
    Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
    RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.

Entry informationi

Entry nameiDHX35_HUMAN
AccessioniPrimary (citable) accession number: Q9H5Z1
Secondary accession number(s): A2RTX3
, B4E0J0, F5GXM6, Q5THR0, Q9H4H7, Q9H6T6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: October 29, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3