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Protein

CUB domain-containing protein 1

Gene

CDCP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.6 Publications

Enzyme and pathway databases

SIGNORiQ9H5V8.

Names & Taxonomyi

Protein namesi
Recommended name:
CUB domain-containing protein 1
Alternative name(s):
Membrane glycoprotein gp140
Subtractive immunization M plus HEp3-associated 135 kDa protein
Short name:
SIMA135
Transmembrane and associated with src kinases
CD_antigen: CD318
Gene namesi
Name:CDCP1
Synonyms:TRASK
ORF Names:UNQ2486/PRO5773
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:24357. CDCP1.

Subcellular locationi

Isoform 1 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 667ExtracellularSequence analysisAdd BLAST638
Transmembranei668 – 688HelicalSequence analysisAdd BLAST21
Topological domaini689 – 836CytoplasmicSequence analysisAdd BLAST148

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi734Y → F: Impaired association with SRC. 1 Publication1
Mutagenesisi762Y → F: Impaired association with protein kinase PRKCG but not with SRC. 1 Publication1

Organism-specific databases

DisGeNETi64866.
OpenTargetsiENSG00000163814.
PharmGKBiPA142672140.

Polymorphism and mutation databases

DMDMi317373455.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 292 PublicationsAdd BLAST29
ChainiPRO_000022624930 – 836CUB domain-containing protein 1Add BLAST807

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...)Sequence analysis1
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Glycosylationi180N-linked (GlcNAc...)Sequence analysis1
Glycosylationi205N-linked (GlcNAc...)Sequence analysis1
Glycosylationi270N-linked (GlcNAc...)Sequence analysis1
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Glycosylationi386N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi476 ↔ 499By similarity
Modified residuei734Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.4 Publications
N-glycosylated.3 Publications
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei368 – 369Cleavage; by ST14/MT-SP12

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9H5V8.
MaxQBiQ9H5V8.
PaxDbiQ9H5V8.
PeptideAtlasiQ9H5V8.
PRIDEiQ9H5V8.

PTM databases

iPTMnetiQ9H5V8.
PhosphoSitePlusiQ9H5V8.
SwissPalmiQ9H5V8.

Expressioni

Tissue specificityi

Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.4 Publications

Gene expression databases

BgeeiENSG00000163814.
CleanExiHS_CDCP1.
GenevisibleiQ9H5V8. HS.

Organism-specific databases

HPAiCAB025637.
HPA010978.
HPA010979.

Interactioni

Subunit structurei

Interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1. Also interacts with SRC and PRKCG/protein kinase C gamma.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SDCBPO005605EBI-1019736,EBI-727004

Protein-protein interaction databases

BioGridi122336. 8 interactors.
DIPiDIP-33861N.
IntActiQ9H5V8. 12 interactors.
MINTiMINT-1346387.
STRINGi9606.ENSP00000296129.

Structurei

3D structure databases

ProteinModelPortaliQ9H5V8.
SMRiQ9H5V8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini417 – 544CUBAdd BLAST128

Sequence similaritiesi

Contains 1 CUB domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJR2. Eukaryota.
ENOG4110CXF. LUCA.
GeneTreeiENSGT00390000010209.
HOVERGENiHBG081070.
InParanoidiQ9H5V8.
KOiK06732.
OMAiSIKRLCI.
OrthoDBiEOG091G01M3.
PhylomeDBiQ9H5V8.
TreeFamiTF331392.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H5V8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGLNCGVSI ALLGVLLLGA ARLPRGAEAF EIALPRESNI TVLIKLGTPT
60 70 80 90 100
LLAKPCYIVI SKRHITMLSI KSGERIVFTF SCQSPENHFV IEIQKNIDCM
110 120 130 140 150
SGPCPFGEVQ LQPSTSLLPT LNRTFIWDVK AHKSIGLELQ FSIPRLRQIG
160 170 180 190 200
PGESCPDGVT HSISGRIDAT VVRIGTFCSN GTVSRIKMQE GVKMALHLPW
210 220 230 240 250
FHPRNVSGFS IANRSSIKRL CIIESVFEGE GSATLMSANY PEGFPEDELM
260 270 280 290 300
TWQFVVPAHL RASVSFLNFN LSNCERKEER VEYYIPGSTT NPEVFKLEDK
310 320 330 340 350
QPGNMAGNFN LSLQGCDQDA QSPGILRLQF QVLVQHPQNE SNKIYVVDLS
360 370 380 390 400
NERAMSLTIE PRPVKQSRKF VPGCFVCLES RTCSSNLTLT SGSKHKISFL
410 420 430 440 450
CDDLTRLWMN VEKTISCTDH RYCQRKSYSL QVPSDILHLP VELHDFSWKL
460 470 480 490 500
LVPKDRLSLV LVPAQKLQQH THEKPCNTSF SYLVASAIPS QDLYFGSFCP
510 520 530 540 550
GGSIKQIQVK QNISVTLRTF APSFQQEASR QGLTVSFIPY FKEEGVFTVT
560 570 580 590 600
PDTKSKVYLR TPNWDRGLPS LTSVSWNISV PRDQVACLTF FKERSGVVCQ
610 620 630 640 650
TGRAFMIIQE QRTRAEEIFS LDEDVLPKPS FHHHSFWVNI SNCSPTSGKQ
660 670 680 690 700
LDLLFSVTLT PRTVDLTVIL IAAVGGGVLL LSALGLIICC VKKKKKKTNK
710 720 730 740 750
GPAVGIYNDN INTEMPRQPK KFQKGRKDND SHVYAVIEDT MVYGHLLQDS
760 770 780 790 800
SGSFLQPEVD TYRPFQGTMG VCPPSPPTIC SRAPTAKLAT EEPPPRSPPE
810 820 830
SESEPYTFSH PNNGDVSSKD TDIPLLNTQE PMEPAE
Length:836
Mass (Da):92,932
Last modified:January 11, 2011 - v3
Checksum:i544FFDBBDD371518
GO
Isoform 2 (identifier: Q9H5V8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-187: Missing.

Note: No experimental confirmation available.
Show »
Length:649
Mass (Da):72,723
Checksum:iCA14C5F721CDCD49
GO
Isoform 3 (identifier: Q9H5V8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     342-343: NK → SE
     344-836: Missing.

Show »
Length:343
Mass (Da):37,818
Checksum:i3E4E13379DD94D1B
GO

Sequence cautioni

The sequence BAB15388 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252W → C in BAB14695 (PubMed:14702039).Curated1
Sequence conflicti284Y → D in BAB15388 (PubMed:14702039).Curated1
Sequence conflicti434S → G in BAB14695 (PubMed:14702039).Curated1
Sequence conflicti466K → R in BAB15388 (PubMed:14702039).Curated1
Sequence conflicti827N → S in AAO33397 (PubMed:12660814).Curated1
Sequence conflicti827N → S in BAB15511 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_025498525Q → R.3 PublicationsCorresponds to variant rs3749191dbSNPEnsembl.1
Natural variantiVAR_055095673A → V.1 PublicationCorresponds to variant rs35428731dbSNPEnsembl.1
Natural variantiVAR_025499709D → G.3 PublicationsCorresponds to variant rs9874077dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0174321 – 187Missing in isoform 2. 1 PublicationAdd BLAST187
Alternative sequenceiVSP_017433342 – 343NK → SE in isoform 3. 2 Publications2
Alternative sequenceiVSP_017434344 – 836Missing in isoform 3. 2 PublicationsAdd BLAST493

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY026461 mRNA. Translation: AAK02058.1.
AF468010 mRNA. Translation: AAO33397.1.
AY167484 mRNA. Translation: AAO34538.1.
AY358779 mRNA. Translation: AAQ89139.1.
AK023834 mRNA. Translation: BAB14695.1.
AK026187 mRNA. Translation: BAB15388.1. Different initiation.
AK026622 mRNA. Translation: BAB15511.1.
AC104165 Genomic DNA. No translation available.
AC105902 Genomic DNA. No translation available.
BC021099 mRNA. Translation: AAH21099.1.
BC069254 mRNA. Translation: AAH69254.1.
AY375452 mRNA. Translation: AAR21289.1.
CCDSiCCDS2727.1. [Q9H5V8-1]
CCDS46812.1. [Q9H5V8-3]
RefSeqiNP_073753.3. NM_022842.4. [Q9H5V8-1]
NP_835488.1. NM_178181.2. [Q9H5V8-3]
UniGeneiHs.476093.

Genome annotation databases

EnsembliENST00000296129; ENSP00000296129; ENSG00000163814. [Q9H5V8-1]
ENST00000425231; ENSP00000399342; ENSG00000163814. [Q9H5V8-3]
GeneIDi64866.
KEGGihsa:64866.
UCSCiuc003com.5. human. [Q9H5V8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY026461 mRNA. Translation: AAK02058.1.
AF468010 mRNA. Translation: AAO33397.1.
AY167484 mRNA. Translation: AAO34538.1.
AY358779 mRNA. Translation: AAQ89139.1.
AK023834 mRNA. Translation: BAB14695.1.
AK026187 mRNA. Translation: BAB15388.1. Different initiation.
AK026622 mRNA. Translation: BAB15511.1.
AC104165 Genomic DNA. No translation available.
AC105902 Genomic DNA. No translation available.
BC021099 mRNA. Translation: AAH21099.1.
BC069254 mRNA. Translation: AAH69254.1.
AY375452 mRNA. Translation: AAR21289.1.
CCDSiCCDS2727.1. [Q9H5V8-1]
CCDS46812.1. [Q9H5V8-3]
RefSeqiNP_073753.3. NM_022842.4. [Q9H5V8-1]
NP_835488.1. NM_178181.2. [Q9H5V8-3]
UniGeneiHs.476093.

3D structure databases

ProteinModelPortaliQ9H5V8.
SMRiQ9H5V8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122336. 8 interactors.
DIPiDIP-33861N.
IntActiQ9H5V8. 12 interactors.
MINTiMINT-1346387.
STRINGi9606.ENSP00000296129.

PTM databases

iPTMnetiQ9H5V8.
PhosphoSitePlusiQ9H5V8.
SwissPalmiQ9H5V8.

Polymorphism and mutation databases

DMDMi317373455.

Proteomic databases

EPDiQ9H5V8.
MaxQBiQ9H5V8.
PaxDbiQ9H5V8.
PeptideAtlasiQ9H5V8.
PRIDEiQ9H5V8.

Protocols and materials databases

DNASUi64866.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296129; ENSP00000296129; ENSG00000163814. [Q9H5V8-1]
ENST00000425231; ENSP00000399342; ENSG00000163814. [Q9H5V8-3]
GeneIDi64866.
KEGGihsa:64866.
UCSCiuc003com.5. human. [Q9H5V8-1]

Organism-specific databases

CTDi64866.
DisGeNETi64866.
GeneCardsiCDCP1.
H-InvDBHIX0003238.
HGNCiHGNC:24357. CDCP1.
HPAiCAB025637.
HPA010978.
HPA010979.
MIMi611735. gene.
neXtProtiNX_Q9H5V8.
OpenTargetsiENSG00000163814.
PharmGKBiPA142672140.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJR2. Eukaryota.
ENOG4110CXF. LUCA.
GeneTreeiENSGT00390000010209.
HOVERGENiHBG081070.
InParanoidiQ9H5V8.
KOiK06732.
OMAiSIKRLCI.
OrthoDBiEOG091G01M3.
PhylomeDBiQ9H5V8.
TreeFamiTF331392.

Enzyme and pathway databases

SIGNORiQ9H5V8.

Miscellaneous databases

ChiTaRSiCDCP1. human.
GeneWikiiCDCP1.
GenomeRNAii64866.
PROiQ9H5V8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163814.
CleanExiHS_CDCP1.
GenevisibleiQ9H5V8. HS.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCDCP1_HUMAN
AccessioniPrimary (citable) accession number: Q9H5V8
Secondary accession number(s): Q49UB4
, Q6NT71, Q6U9Y2, Q8WU91, Q96QU7, Q9H676, Q9H8C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.