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Protein

Vesicular inhibitory amino acid transporter

Gene

SLC32A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the uptake of GABA and glycine into the synaptic vesicles.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

BioCyciZFISH:MONOMER66-34359.
ReactomeiR-HSA-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.
R-HSA-888590. GABA synthesis, release, reuptake and degradation.

Protein family/group databases

TCDBi2.A.18.5.4. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular inhibitory amino acid transporter
Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name:
hVIAAT
Gene namesi
Name:SLC32A1
Synonyms:VGAT, VIAAT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:11018. SLC32A1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 133CytoplasmicSequence analysisAdd BLAST133
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Topological domaini155 – 204Lumenal, vesicleSequence analysisAdd BLAST50
Transmembranei205 – 225HelicalSequence analysisAdd BLAST21
Topological domaini226 – 242CytoplasmicSequence analysisAdd BLAST17
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 265Lumenal, vesicleSequence analysis2
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Topological domaini287 – 305CytoplasmicSequence analysisAdd BLAST19
Transmembranei306 – 326HelicalSequence analysisAdd BLAST21
Topological domaini327 – 341Lumenal, vesicleSequence analysisAdd BLAST15
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 383CytoplasmicSequence analysisAdd BLAST21
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Topological domaini405 – 438Lumenal, vesicleSequence analysisAdd BLAST34
Transmembranei439 – 459HelicalSequence analysisAdd BLAST21
Topological domaini460 – 461CytoplasmicSequence analysis2
Transmembranei462 – 482HelicalSequence analysisAdd BLAST21
Topological domaini483 – 489Lumenal, vesicleSequence analysis7
Transmembranei490 – 510HelicalSequence analysisAdd BLAST21
Topological domaini511 – 525CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi140679.
OpenTargetsiENSG00000101438.
PharmGKBiPA401.

Chemistry databases

DrugBankiDB00145. Glycine.
GuidetoPHARMACOLOGYi1133.

Polymorphism and mutation databases

BioMutaiSLC32A1.
DMDMi29428257.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938201 – 525Vesicular inhibitory amino acid transporterAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186Nitrated tyrosineBy similarity1
Glycosylationi341N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Nitration

Proteomic databases

PaxDbiQ9H598.
PeptideAtlasiQ9H598.
PRIDEiQ9H598.

PTM databases

iPTMnetiQ9H598.
PhosphoSitePlusiQ9H598.

Expressioni

Tissue specificityi

Retina. Expressed throughout the horizontal cells or more specifically at the terminals.1 Publication

Gene expression databases

BgeeiENSG00000101438.
CleanExiHS_SLC32A1.
GenevisibleiQ9H598. HS.

Organism-specific databases

HPAiCAB037156.
HPA058859.
HPA059985.

Interactioni

Protein-protein interaction databases

BioGridi126644. 2 interactors.
IntActiQ9H598. 1 interactor.
STRINGi9606.ENSP00000217420.

Structurei

3D structure databases

ProteinModelPortaliQ9H598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4303. Eukaryota.
COG0814. LUCA.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000220837.
HOVERGENiHBG061364.
InParanoidiQ9H598.
KOiK15015.
OMAiANACCSP.
OrthoDBiEOG091G05MY.
PhylomeDBiQ9H598.
TreeFamiTF312818.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLLRSKLS NVATSVSNKS QAKMSGMFAR MGFQAATDEE AVGFAHCDDL
60 70 80 90 100
DFEHRQGLQM DILKAEGEPC GDEGAEAPVE GDIHYQRGSG APLPPSGSKD
110 120 130 140 150
QVGGGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF
160 170 180 190 200
LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT
210 220 230 240 250
LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV
260 270 280 290 300
LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY
310 320 330 340 350
IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL
360 370 380 390 400
KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNIFLVAKA LLSYPLPFFA
410 420 430 440 450
AVEVLEKSLF QEGSRAFFPA CYSGDGRLKS WGLTLRCALV VFTLLMAIYV
460 470 480 490 500
PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG
510 520
GICSVSGFVH SLEGLIEAYR TNAED
Length:525
Mass (Da):57,415
Last modified:June 1, 2001 - v2
Checksum:iC3C4B0786B8DE745
GO

Sequence cautioni

The sequence AAH36458 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti261K → R in AAH36458 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048121423S → G.Corresponds to variant rs34517228dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY044836 mRNA. Translation: AAK98782.1.
AK055051 mRNA. Translation: BAB70846.1.
AL133519 Genomic DNA. Translation: CAC15529.2.
BC036458 mRNA. Translation: AAH36458.2. Different initiation.
BC053582 mRNA. Translation: AAH53582.1.
CCDSiCCDS13307.1.
RefSeqiNP_542119.1. NM_080552.2.
UniGeneiHs.179080.

Genome annotation databases

EnsembliENST00000217420; ENSP00000217420; ENSG00000101438.
GeneIDi140679.
KEGGihsa:140679.
UCSCiuc002xjc.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY044836 mRNA. Translation: AAK98782.1.
AK055051 mRNA. Translation: BAB70846.1.
AL133519 Genomic DNA. Translation: CAC15529.2.
BC036458 mRNA. Translation: AAH36458.2. Different initiation.
BC053582 mRNA. Translation: AAH53582.1.
CCDSiCCDS13307.1.
RefSeqiNP_542119.1. NM_080552.2.
UniGeneiHs.179080.

3D structure databases

ProteinModelPortaliQ9H598.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126644. 2 interactors.
IntActiQ9H598. 1 interactor.
STRINGi9606.ENSP00000217420.

Chemistry databases

DrugBankiDB00145. Glycine.
GuidetoPHARMACOLOGYi1133.

Protein family/group databases

TCDBi2.A.18.5.4. the amino acid/auxin permease (aaap) family.

PTM databases

iPTMnetiQ9H598.
PhosphoSitePlusiQ9H598.

Polymorphism and mutation databases

BioMutaiSLC32A1.
DMDMi29428257.

Proteomic databases

PaxDbiQ9H598.
PeptideAtlasiQ9H598.
PRIDEiQ9H598.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000217420; ENSP00000217420; ENSG00000101438.
GeneIDi140679.
KEGGihsa:140679.
UCSCiuc002xjc.3. human.

Organism-specific databases

CTDi140679.
DisGeNETi140679.
GeneCardsiSLC32A1.
HGNCiHGNC:11018. SLC32A1.
HPAiCAB037156.
HPA058859.
HPA059985.
MIMi616440. gene.
neXtProtiNX_Q9H598.
OpenTargetsiENSG00000101438.
PharmGKBiPA401.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4303. Eukaryota.
COG0814. LUCA.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000220837.
HOVERGENiHBG061364.
InParanoidiQ9H598.
KOiK15015.
OMAiANACCSP.
OrthoDBiEOG091G05MY.
PhylomeDBiQ9H598.
TreeFamiTF312818.

Enzyme and pathway databases

BioCyciZFISH:MONOMER66-34359.
ReactomeiR-HSA-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.
R-HSA-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

GeneWikiiSLC32A1.
GenomeRNAii140679.
PROiQ9H598.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101438.
CleanExiHS_SLC32A1.
GenevisibleiQ9H598. HS.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVIAAT_HUMAN
AccessioniPrimary (citable) accession number: Q9H598
Secondary accession number(s): Q8N489
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.