Q9H598 (VIAAT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicular inhibitory amino acid transporter

Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name=hVIAAT

Gene names

Name:	SLC32A1
Synonyms:	VGAT, VIAAT

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the uptake of GABA and glycine into the synaptic vesicles.
Subcellular location	Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Retina. Expressed throughout the horizontal cells or more specifically at the terminals. Ref.5
Sequence similarities	Belongs to the amino acid/polyamine transporter 2 family.
Sequence caution	The sequence AAH36458.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Neurotransmitter transport Transport
Cellular component	Cytoplasmic vesicle Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from electronic annotation. Source: Compara ion transport Traceable author statement. Source: Reactome neurotransmitter secretion Traceable author statement. Source: Reactome transmembrane transport Traceable author statement. Source: Reactome
Cellular_component	cell tip Inferred from electronic annotation. Source: Compara clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane Traceable author statement. Source: Reactome cone cell pedicle Inferred from electronic annotation. Source: Compara dendrite Inferred from direct assay Ref.5. Source: MGI dendrite terminus Inferred from electronic annotation. Source: Compara inhibitory synapse Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection terminus Inferred from direct assay Ref.5. Source: MGI plasma membrane Traceable author statement. Source: Reactome synaptic vesicle membrane Traceable author statement. Source: Reactome
Molecular_function	gamma-aminobutyric acid:hydrogen symporter activity Inferred from electronic annotation. Source: Compara glycine transmembrane transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Vesicular inhibitory amino acid transporter		PRO_0000093820
Regions
	Topological domain	1 – 133	133	Cytoplasmic Potential
	Transmembrane	134 – 154	21	Helical; Potential
	Topological domain	155 – 204	50	Lumenal, vesicle Potential
	Transmembrane	205 – 225	21	Helical; Potential
	Topological domain	226 – 242	17	Cytoplasmic Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Topological domain	264 – 265	2	Lumenal, vesicle Potential
	Transmembrane	266 – 286	21	Helical; Potential
	Topological domain	287 – 305	19	Cytoplasmic Potential
	Transmembrane	306 – 326	21	Helical; Potential
	Topological domain	327 – 341	15	Lumenal, vesicle Potential
	Transmembrane	342 – 362	21	Helical; Potential
	Topological domain	363 – 383	21	Cytoplasmic Potential
	Transmembrane	384 – 404	21	Helical; Potential
	Topological domain	405 – 438	34	Lumenal, vesicle Potential
	Transmembrane	439 – 459	21	Helical; Potential
	Topological domain	460 – 461	2	Cytoplasmic Potential
	Transmembrane	462 – 482	21	Helical; Potential
	Topological domain	483 – 489	7	Lumenal, vesicle Potential
	Transmembrane	490 – 510	21	Helical; Potential
	Topological domain	511 – 525	15	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	341	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	423	1	S → G. Corresponds to variant rs34517228 [ dbSNP | Ensembl ].		VAR_048121
Experimental info
	Sequence conflict	261	1	K → R in AAH36458. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9H598 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: C3C4B0786B8DE745	FASTA	525	57,415
	10 20 30 40 50 60 MATLLRSKLS NVATSVSNKS QAKMSGMFAR MGFQAATDEE AVGFAHCDDL DFEHRQGLQM 70 80 90 100 110 120 DILKAEGEPC GDEGAEAPVE GDIHYQRGSG APLPPSGSKD QVGGGGEFGG HDKPKITAWE 130 140 150 160 170 180 AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF LIIFAAVVCC YTGKILIACL YEENEDGEVV 190 200 210 220 230 240 RVRDSYVAIA NACCAPRFPT LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ 250 260 270 280 290 300 KSWSIIATAV LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY 310 320 330 340 350 360 IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL KGLFALVAYL 370 380 390 400 410 420 TWADETKEVI TDNLPGSIRA VVNIFLVAKA LLSYPLPFFA AVEVLEKSLF QEGSRAFFPA 430 440 450 460 470 480 CYSGDGRLKS WGLTLRCALV VFTLLMAIYV PHFALLMGLT GSLTGAGLCF LLPSLFHLRL 490 500 510 520 LWRKLLWHQV FFDVAIFVIG GICSVSGFVH SLEGLIEAYR TNAED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of the vesicular inhibitory amino acid transporter in pancreatic islet cells: distribution of the transporter within rat islets." Chessler S.D., Simonson W.T., Sweet I.R., Hammerle L.P. Diabetes 51:1763-1771(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Cellular localization of the vesicular inhibitory amino acid transporter in the mouse and human retina." Jellali A., Stussi-Garaud C., Gasnier B., Rendon A., Sahel J.-A., Dreyfus H., Picaud S. J. Comp. Neurol. 449:76-87(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY044836 mRNA. Translation: AAK98782.1. AK055051 mRNA. Translation: BAB70846.1. AL133519 Genomic DNA. Translation: CAC15529.2. BC036458 mRNA. Translation: AAH36458.2. Different initiation. BC053582 mRNA. Translation: AAH53582.1.
IPI	IPI00026015.
RefSeq	NP_542119.1. NM_080552.2.
UniGene	Hs.179080.
3D structure databases
ProteinModelPortal	Q9H598.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000217420.
PTM databases
PhosphoSite	Q9H598.
Polymorphism databases
DMDM	29428257.
Proteomic databases
PaxDb	Q9H598.
PeptideAtlas	Q9H598.
PRIDE	Q9H598.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000217420; ENSP00000217420; ENSG00000101438.
GeneID	140679.
KEGG	hsa:140679.
UCSC	uc002xjc.3. human.
Organism-specific databases
CTD	140679.
GeneCards	GC20P037353.
HGNC	HGNC:11018. SLC32A1.
HPA	CAB037156.
neXtProt	NX_Q9H598.
PharmGKB	PA401.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG294245.
HOGENOM	HOG000220837.
HOVERGEN	HBG061364.
InParanoid	Q9H598.
KO	K15015.
OMA	QMDILKS.
OrthoDB	EOG4HMJ91.
PhylomeDB	Q9H598.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System. REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters.
Gene expression databases
Bgee	Q9H598.
CleanEx	HS_SLC32A1.
Genevestigator	Q9H598.
GermOnline	ENSG00000101438. Homo sapiens.
Family and domain databases
InterPro	IPR013057. AA_transpt_TM. [Graphical view]
Pfam	PF01490. Aa_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL4536.
DrugBank	DB00145. Glycine.
GenomeRNAi	140679.
NextBio	84209.

Entry information

Entry name

VIAAT_HUMAN

Accession

Primary (citable) accession number: Q9H598
Secondary accession number(s): Q8N489

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.