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Q9H598

- VIAAT_HUMAN

UniProt

Q9H598 - VIAAT_HUMAN

Protein

Vesicular inhibitory amino acid transporter

Gene

SLC32A1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Involved in the uptake of GABA and glycine into the synaptic vesicles.

    GO - Molecular functioni

    1. gamma-aminobutyric acid:proton symporter activity Source: Ensembl
    2. glycine transmembrane transporter activity Source: Ensembl

    GO - Biological processi

    1. aging Source: Ensembl
    2. ion transport Source: Reactome
    3. neurotransmitter secretion Source: Reactome
    4. synaptic transmission Source: Reactome
    5. transmembrane transport Source: Reactome

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.
    REACT_23947. GABA synthesis, release, reuptake and degradation.

    Protein family/group databases

    TCDBi2.A.18.5.4. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicular inhibitory amino acid transporter
    Alternative name(s):
    GABA and glycine transporter
    Solute carrier family 32 member 1
    Vesicular GABA transporter
    Short name:
    hVIAAT
    Gene namesi
    Name:SLC32A1
    Synonyms:VGAT, VIAAT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:11018. SLC32A1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell tip Source: Ensembl
    2. clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane Source: Reactome
    3. cone cell pedicle Source: Ensembl
    4. dendrite Source: MGI
    5. dendrite terminus Source: Ensembl
    6. inhibitory synapse Source: Ensembl
    7. integral component of membrane Source: UniProtKB-KW
    8. neuron projection Source: MGI
    9. neuron projection terminus Source: MGI
    10. plasma membrane Source: Reactome
    11. synaptic vesicle membrane Source: Reactome

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA401.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 525525Vesicular inhibitory amino acid transporterPRO_0000093820Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei186 – 1861Nitrated tyrosineBy similarity
    Glycosylationi341 – 3411N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Nitration

    Proteomic databases

    PaxDbiQ9H598.
    PeptideAtlasiQ9H598.
    PRIDEiQ9H598.

    PTM databases

    PhosphoSiteiQ9H598.

    Expressioni

    Tissue specificityi

    Retina. Expressed throughout the horizontal cells or more specifically at the terminals.1 Publication

    Gene expression databases

    BgeeiQ9H598.
    CleanExiHS_SLC32A1.
    GenevestigatoriQ9H598.

    Organism-specific databases

    HPAiCAB037156.
    HPA058859.
    HPA059985.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000217420.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H598.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 133133CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini155 – 20450Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini226 – 24217CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini264 – 2652Lumenal, vesicleSequence Analysis
    Topological domaini287 – 30519CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini327 – 34115Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini363 – 38321CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini405 – 43834Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini460 – 4612CytoplasmicSequence Analysis
    Topological domaini483 – 4897Lumenal, vesicleSequence Analysis
    Topological domaini511 – 52515CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei134 – 15421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei205 – 22521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei266 – 28621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei306 – 32621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei384 – 40421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei439 – 45921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei462 – 48221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei490 – 51021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG294245.
    HOGENOMiHOG000220837.
    HOVERGENiHBG061364.
    InParanoidiQ9H598.
    KOiK15015.
    OMAiMYNSFPN.
    OrthoDBiEOG7SR4M2.
    PhylomeDBiQ9H598.
    TreeFamiTF312818.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9H598-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATLLRSKLS NVATSVSNKS QAKMSGMFAR MGFQAATDEE AVGFAHCDDL    50
    DFEHRQGLQM DILKAEGEPC GDEGAEAPVE GDIHYQRGSG APLPPSGSKD 100
    QVGGGGEFGG HDKPKITAWE AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF 150
    LIIFAAVVCC YTGKILIACL YEENEDGEVV RVRDSYVAIA NACCAPRFPT 200
    LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ KSWSIIATAV 250
    LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY 300
    IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL 350
    KGLFALVAYL TWADETKEVI TDNLPGSIRA VVNIFLVAKA LLSYPLPFFA 400
    AVEVLEKSLF QEGSRAFFPA CYSGDGRLKS WGLTLRCALV VFTLLMAIYV 450
    PHFALLMGLT GSLTGAGLCF LLPSLFHLRL LWRKLLWHQV FFDVAIFVIG 500
    GICSVSGFVH SLEGLIEAYR TNAED 525
    Length:525
    Mass (Da):57,415
    Last modified:June 1, 2001 - v2
    Checksum:iC3C4B0786B8DE745
    GO

    Sequence cautioni

    The sequence AAH36458.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti261 – 2611K → R in AAH36458. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti423 – 4231S → G.
    Corresponds to variant rs34517228 [ dbSNP | Ensembl ].
    VAR_048121

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY044836 mRNA. Translation: AAK98782.1.
    AK055051 mRNA. Translation: BAB70846.1.
    AL133519 Genomic DNA. Translation: CAC15529.2.
    BC036458 mRNA. Translation: AAH36458.2. Different initiation.
    BC053582 mRNA. Translation: AAH53582.1.
    CCDSiCCDS13307.1.
    RefSeqiNP_542119.1. NM_080552.2.
    UniGeneiHs.179080.

    Genome annotation databases

    EnsembliENST00000217420; ENSP00000217420; ENSG00000101438.
    GeneIDi140679.
    KEGGihsa:140679.
    UCSCiuc002xjc.3. human.

    Polymorphism databases

    DMDMi29428257.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY044836 mRNA. Translation: AAK98782.1 .
    AK055051 mRNA. Translation: BAB70846.1 .
    AL133519 Genomic DNA. Translation: CAC15529.2 .
    BC036458 mRNA. Translation: AAH36458.2 . Different initiation.
    BC053582 mRNA. Translation: AAH53582.1 .
    CCDSi CCDS13307.1.
    RefSeqi NP_542119.1. NM_080552.2.
    UniGenei Hs.179080.

    3D structure databases

    ProteinModelPortali Q9H598.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000217420.

    Chemistry

    DrugBanki DB00145. Glycine.
    GuidetoPHARMACOLOGYi 1133.

    Protein family/group databases

    TCDBi 2.A.18.5.4. the amino acid/auxin permease (aaap) family.

    PTM databases

    PhosphoSitei Q9H598.

    Polymorphism databases

    DMDMi 29428257.

    Proteomic databases

    PaxDbi Q9H598.
    PeptideAtlasi Q9H598.
    PRIDEi Q9H598.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000217420 ; ENSP00000217420 ; ENSG00000101438 .
    GeneIDi 140679.
    KEGGi hsa:140679.
    UCSCi uc002xjc.3. human.

    Organism-specific databases

    CTDi 140679.
    GeneCardsi GC20P037353.
    HGNCi HGNC:11018. SLC32A1.
    HPAi CAB037156.
    HPA058859.
    HPA059985.
    neXtProti NX_Q9H598.
    PharmGKBi PA401.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG294245.
    HOGENOMi HOG000220837.
    HOVERGENi HBG061364.
    InParanoidi Q9H598.
    KOi K15015.
    OMAi MYNSFPN.
    OrthoDBi EOG7SR4M2.
    PhylomeDBi Q9H598.
    TreeFami TF312818.

    Enzyme and pathway databases

    Reactomei REACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.
    REACT_23947. GABA synthesis, release, reuptake and degradation.

    Miscellaneous databases

    GeneWikii SLC32A1.
    GenomeRNAii 140679.
    NextBioi 84209.
    PROi Q9H598.

    Gene expression databases

    Bgeei Q9H598.
    CleanExi HS_SLC32A1.
    Genevestigatori Q9H598.

    Family and domain databases

    InterProi IPR013057. AA_transpt_TM.
    [Graphical view ]
    Pfami PF01490. Aa_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of the vesicular inhibitory amino acid transporter in pancreatic islet cells: distribution of the transporter within rat islets."
      Chessler S.D., Simonson W.T., Sweet I.R., Hammerle L.P.
      Diabetes 51:1763-1771(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Cellular localization of the vesicular inhibitory amino acid transporter in the mouse and human retina."
      Jellali A., Stussi-Garaud C., Gasnier B., Rendon A., Sahel J.-A., Dreyfus H., Picaud S.
      J. Comp. Neurol. 449:76-87(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiVIAAT_HUMAN
    AccessioniPrimary (citable) accession number: Q9H598
    Secondary accession number(s): Q8N489
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3