Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9H598 (VIAAT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicular inhibitory amino acid transporter
Alternative name(s):
GABA and glycine transporter
Solute carrier family 32 member 1
Vesicular GABA transporter
Short name=hVIAAT
Gene names
Name:SLC32A1
Synonyms:VGAT, VIAAT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the uptake of GABA and glycine into the synaptic vesicles.

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Retina. Expressed throughout the horizontal cells or more specifically at the terminals. Ref.5

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Sequence caution

The sequence AAH36458.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processNeurotransmitter transport
Transport
   Cellular componentCytoplasmic vesicle
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Nitration
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from electronic annotation. Source: Ensembl

ion transport

Traceable author statement. Source: Reactome

neurotransmitter secretion

Traceable author statement. Source: Reactome

synaptic transmission

Traceable author statement. Source: Reactome

transmembrane transport

Traceable author statement. Source: Reactome

   Cellular_componentcell tip

Inferred from electronic annotation. Source: Ensembl

clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane

Traceable author statement. Source: Reactome

cone cell pedicle

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from direct assay Ref.5. Source: MGI

dendrite terminus

Inferred from electronic annotation. Source: Ensembl

inhibitory synapse

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuron projection

Inferred from direct assay Ref.5. Source: MGI

neuron projection terminus

Inferred from direct assay Ref.5. Source: MGI

plasma membrane

Traceable author statement. Source: Reactome

synaptic vesicle membrane

Traceable author statement. Source: Reactome

   Molecular_functiongamma-aminobutyric acid:proton symporter activity

Inferred from electronic annotation. Source: Ensembl

glycine transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Vesicular inhibitory amino acid transporter
PRO_0000093820

Regions

Topological domain1 – 133133Cytoplasmic Potential
Transmembrane134 – 15421Helical; Potential
Topological domain155 – 20450Lumenal, vesicle Potential
Transmembrane205 – 22521Helical; Potential
Topological domain226 – 24217Cytoplasmic Potential
Transmembrane243 – 26321Helical; Potential
Topological domain264 – 2652Lumenal, vesicle Potential
Transmembrane266 – 28621Helical; Potential
Topological domain287 – 30519Cytoplasmic Potential
Transmembrane306 – 32621Helical; Potential
Topological domain327 – 34115Lumenal, vesicle Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 38321Cytoplasmic Potential
Transmembrane384 – 40421Helical; Potential
Topological domain405 – 43834Lumenal, vesicle Potential
Transmembrane439 – 45921Helical; Potential
Topological domain460 – 4612Cytoplasmic Potential
Transmembrane462 – 48221Helical; Potential
Topological domain483 – 4897Lumenal, vesicle Potential
Transmembrane490 – 51021Helical; Potential
Topological domain511 – 52515Cytoplasmic Potential

Amino acid modifications

Modified residue1861Nitrated tyrosine By similarity
Glycosylation3411N-linked (GlcNAc...) Potential

Natural variations

Natural variant4231S → G.
Corresponds to variant rs34517228 [ dbSNP | Ensembl ].
VAR_048121

Experimental info

Sequence conflict2611K → R in AAH36458. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9H598 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: C3C4B0786B8DE745

FASTA52557,415
        10         20         30         40         50         60 
MATLLRSKLS NVATSVSNKS QAKMSGMFAR MGFQAATDEE AVGFAHCDDL DFEHRQGLQM 

        70         80         90        100        110        120 
DILKAEGEPC GDEGAEAPVE GDIHYQRGSG APLPPSGSKD QVGGGGEFGG HDKPKITAWE 

       130        140        150        160        170        180 
AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF LIIFAAVVCC YTGKILIACL YEENEDGEVV 

       190        200        210        220        230        240 
RVRDSYVAIA NACCAPRFPT LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ 

       250        260        270        280        290        300 
KSWSIIATAV LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY 

       310        320        330        340        350        360 
IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL KGLFALVAYL 

       370        380        390        400        410        420 
TWADETKEVI TDNLPGSIRA VVNIFLVAKA LLSYPLPFFA AVEVLEKSLF QEGSRAFFPA 

       430        440        450        460        470        480 
CYSGDGRLKS WGLTLRCALV VFTLLMAIYV PHFALLMGLT GSLTGAGLCF LLPSLFHLRL 

       490        500        510        520 
LWRKLLWHQV FFDVAIFVIG GICSVSGFVH SLEGLIEAYR TNAED 

« Hide

References

« Hide 'large scale' references
[1]"Expression of the vesicular inhibitory amino acid transporter in pancreatic islet cells: distribution of the transporter within rat islets."
Chessler S.D., Simonson W.T., Sweet I.R., Hammerle L.P.
Diabetes 51:1763-1771(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Cellular localization of the vesicular inhibitory amino acid transporter in the mouse and human retina."
Jellali A., Stussi-Garaud C., Gasnier B., Rendon A., Sahel J.-A., Dreyfus H., Picaud S.
J. Comp. Neurol. 449:76-87(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY044836 mRNA. Translation: AAK98782.1.
AK055051 mRNA. Translation: BAB70846.1.
AL133519 Genomic DNA. Translation: CAC15529.2.
BC036458 mRNA. Translation: AAH36458.2. Different initiation.
BC053582 mRNA. Translation: AAH53582.1.
CCDSCCDS13307.1.
RefSeqNP_542119.1. NM_080552.2.
UniGeneHs.179080.

3D structure databases

ProteinModelPortalQ9H598.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000217420.

Chemistry

DrugBankDB00145. Glycine.
GuidetoPHARMACOLOGY1133.

Protein family/group databases

TCDB2.A.18.5.4. the amino acid/auxin permease (aaap) family.

PTM databases

PhosphoSiteQ9H598.

Polymorphism databases

DMDM29428257.

Proteomic databases

PaxDbQ9H598.
PeptideAtlasQ9H598.
PRIDEQ9H598.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000217420; ENSP00000217420; ENSG00000101438.
GeneID140679.
KEGGhsa:140679.
UCSCuc002xjc.3. human.

Organism-specific databases

CTD140679.
GeneCardsGC20P037353.
HGNCHGNC:11018. SLC32A1.
HPACAB037156.
HPA058859.
HPA059985.
neXtProtNX_Q9H598.
PharmGKBPA401.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG294245.
HOGENOMHOG000220837.
HOVERGENHBG061364.
InParanoidQ9H598.
KOK15015.
OMAMYNSFPN.
OrthoDBEOG7SR4M2.
PhylomeDBQ9H598.
TreeFamTF312818.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_19419. Amino acid and oligopeptide SLC transporters.

Gene expression databases

BgeeQ9H598.
CleanExHS_SLC32A1.
GenevestigatorQ9H598.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSLC32A1.
GenomeRNAi140679.
NextBio84209.
PROQ9H598.

Entry information

Entry nameVIAAT_HUMAN
AccessionPrimary (citable) accession number: Q9H598
Secondary accession number(s): Q8N489
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM