Reviewed, UniProtKB/Swiss-Prot Q9H4X1 (RGC32_HUMAN)

Names and origin

Protein names

Recommended name:
    Response gene to complement 32 protein
      Short name=RGC-32

Gene names

Name:	RGC32
Synonyms:	C13orf15

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Modulates the activity of cell cycle-specific kinases. Enhances CDC2 activity. May contribute to the regulation of the cell cycle. May inhibit growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. Ref.1 Ref.5
Subunit structure	Interacts with CDC2 and PLK1. Ref.5
Subcellular location	Cytoplasm. Nucleus. Centrosome. Note: Cytoplasmic in unstimulated cells. Nuclear after activation by complement. Associated with the centrosome during prometaphase and metaphase. Ref.1 Ref.5
Tissue specificity	Detected in brain, heart and liver (at protein level). Highly expressed in liver, skeletal muscle, kidney and pancreas. Detected at lower levels in heart, brain and placenta. Detected in aorta endothelial cells. Ref.1
Induction	Up-regulated in aorta endothelial cells in response to complement activation. Ref.1

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW regulation of cyclin-dependent protein kinase activity Traceable author statement. Source: ProtInc
Cellular component	centrosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H4X1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H4X1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     6-25: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	Response gene to complement 32 protein		PRO_0000274701
Regions
	Compositional bias	11 – 26	16	Ala-rich
	Compositional bias	64 – 109	46	Ser/Thr-rich
Amino acid modifications
	Modified residue	9	1	Phosphoserine Ref.4
	Modified residue	29	1	Phosphoserine Ref.4
	Modified residue	44	1	Phosphoserine Ref.4
	Modified residue	111	1	Phosphothreonine; by CDC2 Ref.1
Natural variations
	Alternative sequence	6 – 25	20	Missing in isoform 2.		VSP_022873
Experimental info
	Mutagenesis	111	1	T → A: Loss of phosphorylation. Reduced stimulation of CDC2 activity. Ref.1
	Sequence conflict	3	1	P → Q in AAH66334. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 76265677DBCD9525	FASTA	137	14,559
	10 20 30 40 50 60 MKPPAAQGSP AAAAAAAPAL DSAAAEDLSD ALCEFDAVLA DFASPFHERH FHYEEHLERM 70 80 90 100 110 120 KRRSSASVSD SSGFSDSESA DSLYRNSFSF SDEKLNSPTD STPALLSATV TPQKAKLGDT 130 KELEAFIADL DKTLASM
	Isoform 2. Checksum: 3F01B45873DE5909	FASTA	117	12,924
	10 20 30 40 50 60 MKPPAEDLSD ALCEFDAVLA DFASPFHERH FHYEEHLERM KRRSSASVSD SSGFSDSESA 70 80 90 100 110 DSLYRNSFSF SDEKLNSPTD STPALLSATV TPQKAKLGDT KELEAFIADL DKTLASM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RGC-32 increases p34CDC2 kinase activity and entry of aortic smooth muscle cells into S-phase." Badea T., Niculescu F., Soane L., Fosbrink M., Sorana H., Rus V., Shin M.L., Rus H. J. Biol. Chem. 277:502-508(2002) [PubMed: 11687586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INDUCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-111, PHOSPHORYLATION AT THR-111, TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; SER-29 AND SER-44, MASS SPECTROMETRY. Tissue: Epithelium.
[5]	"RGC32, a novel p53-inducible gene, is located on centrosomes during mitosis and results in G2/M arrest." Saigusa K., Imoto I., Tanikawa C., Aoyagi M., Ohno K., Nakamura Y., Inazawa J. Oncogene 26:1110-1121(2007) [PubMed: 17146433] [Abstract] Cited for: INTERACTION WITH PLK1, FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF036549 mRNA. Translation: AAF04336.1. AL354833 Genomic DNA. Translation: CAC13101.1. BC066334 mRNA. Translation: AAH66334.1.
IPI	IPI00022350. IPI00827579.
RefSeq	NP_054778.2.
UniGene	Hs.507866
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q9H4X1.
Genome annotation databases
Ensembl	ENSG00000102760. Homo sapiens. [Contig view]
GeneID	28984.
KEGG	hsa:28984.
Organism-specific databases
GeneCards	GC13P040932.
HGNC	HGNC:20369. C13orf15.
MIM	610077. gene.
PharmGKB	PA134895181.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9H4X1.
OMA	Q9H4X1. FRYDEHL.
Gene expression databases
ArrayExpress	Q9H4X1.
Bgee	Q9H4X1.
CleanEx	HS_C13orf15.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	51881.
SOURCE	Search...

Entry information

Entry name

RGC32_HUMAN

Accession

Primary (citable) accession number: Q9H4X1
Secondary accession number(s): Q6NZ48, Q9UL69

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)