Q9H4W6 (COE3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor COE3

Alternative name(s):
Early B-cell factor 3
Short name=EBF-3
Olf-1/EBF-like 2
Short name=O/E-2
Short name=OE-2

Gene names

Name:	EBF3
Synonyms:	COE3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' By similarity.
Subunit structure	Forms either a homodimer or a heterodimer with a related family member By similarity.
Subcellular location	Nucleus Potential.
Tissue specificity	Expressed in brain. Ref.4
Sequence similarities	Belongs to the COE family. Contains 1 IPT/TIG domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Developmental protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MEN1	O00255	1	EBI-2111898,EBI-592789

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q9H4W6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q9H4W6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     252-260: Missing.
     521-558: IVPSSPTMAASSVTLPSNCSSTHGIFSFSPANVISAVK → MK

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 596

596

Transcription factor COE3

PRO_0000107832

Regions

Domain

263 – 346

84

IPT/TIG

Zinc finger

151 – 170

20

C5-type Potential

Region

63 – 66

4

Interaction with DNA By similarity

Region

197 – 204

8

Interaction with DNA By similarity

Region

236 – 239

4

Interaction with DNA By similarity

Compositional bias

464 – 555

92

Pro/Ser/Thr-rich

Sites

Site

163

1

Interaction with DNA By similarity

Site

172

1

Interaction with DNA By similarity

Natural variations

Alternative sequence

252 – 260

9

Missing in isoform Short.

VSP_001113

Alternative sequence

521 – 558

38

IVPSS…ISAVK → MK in isoform Short.

VSP_001114

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

596

Helix Strand Turn

Details...

Beta strand

267 – 270

4

Beta strand

280 – 284

5

Beta strand

293 – 295

3

Beta strand

303 – 307

5

Beta strand

310 – 314

5

Beta strand

327 – 330

4

Beta strand

333 – 335

3

Helix

358 – 363

6

Helix

376 – 390

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified May 4, 2001. Version 2. Checksum: EE5C9D10CD3DED02	FASTA	596	64,864
	10 20 30 40 50 60 MFGIQENIPR GGTTMKEEPL GSGMNPVRSW MHTAGVVDAN TAAQSGVGLA RAHFEKQPPS 70 80 90 100 110 120 NLRKSNFFHF VLALYDRQGQ PVEIERTAFV DFVEKEKEPN NEKTNNGIHY KLQLLYSNGV 130 140 150 160 170 180 RTEQDLYVRL IDSMTKQAIV YEGQDKNPEM CRVLLTHEIM CSRCCDKKSC GNRNETPSDP 190 200 210 220 230 240 VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH 250 260 270 280 290 300 GRRARRLDPS EGTAPSYLEN ATPCIKAISP SEGWTTGGAT VIIIGDNFFD GLQVVFGTML 310 320 330 340 350 360 VWSELITPHA IRVQTPPRHI PGVVEVTLSY KSKQFCKGAP GRFVYTALNE PTIDYGFQRL 370 380 390 400 410 420 QKVIPRHPGD PERLPKEVLL KRAADLVEAL YGMPHNNQEI ILKRAADIAE ALYSVPRNHN 430 440 450 460 470 480 QIPTLGNNPA HTGMMGVNSF SSQLAVNVSE TSQANDQVGY SRNTSSVSPR GYVPSSTPQQ 490 500 510 520 530 540 SNYNTVSTSM NGYGSGAMAS LGVPGSPGFL NGSSANSPYG IVPSSPTMAA SSVTLPSNCS 550 560 570 580 590 STHGIFSFSP ANVISAVKQK SAFAPVVRPQ ASPPPSCTSA NGNGLQAMSG LVVPPM
	Isoform Short [UniParc]. Checksum: 0813B855111FAB12	FASTA	551	60,415
	10 20 30 40 50 60 MFGIQENIPR GGTTMKEEPL GSGMNPVRSW MHTAGVVDAN TAAQSGVGLA RAHFEKQPPS 70 80 90 100 110 120 NLRKSNFFHF VLALYDRQGQ PVEIERTAFV DFVEKEKEPN NEKTNNGIHY KLQLLYSNGV 130 140 150 160 170 180 RTEQDLYVRL IDSMTKQAIV YEGQDKNPEM CRVLLTHEIM CSRCCDKKSC GNRNETPSDP 190 200 210 220 230 240 VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH 250 260 270 280 290 300 GRRARRLDPS EATPCIKAIS PSEGWTTGGA TVIIIGDNFF DGLQVVFGTM LVWSELITPH 310 320 330 340 350 360 AIRVQTPPRH IPGVVEVTLS YKSKQFCKGA PGRFVYTALN EPTIDYGFQR LQKVIPRHPG 370 380 390 400 410 420 DPERLPKEVL LKRAADLVEA LYGMPHNNQE IILKRAADIA EALYSVPRNH NQIPTLGNNP 430 440 450 460 470 480 AHTGMMGVNS FSSQLAVNVS ETSQANDQVG YSRNTSSVSP RGYVPSSTPQ QSNYNTVSTS 490 500 510 520 530 540 MNGYGSGAMA SLGVPGSPGF LNGSSANSPY GMKQKSAFAP VVRPQASPPP SCTSANGNGL 550 QAMSGLVVPP M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS LONG AND SHORT).
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
[4]	"Integrated genomic and epigenomic analyses pinpoint biallelic gene inactivation in tumors." Zardo G., Tiirikainen M.I., Hong C., Misra A., Feuerstein B.G., Volik S., Collins C.C., Lamborn K.R., Bollen A., Pinkel D., Albertson D.G., Costello J.F. Nat. Genet. 32:453-458(2002) [PubMed: 12355068] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Structural determination of functional domains in early B-cell factor (EBF) family of transcription factors reveals similarities to Rel DNA-binding proteins and a novel dimerization motif." Siponen M.I., Wisniewska M., Lehtio L., Johansson I., Svensson L., Raszewski G., Nilsson L., Sigvardsson M., Berglund H. J. Biol. Chem. 285:25875-25879(2010) [PubMed: 20592035] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 261-415.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL354950 Genomic DNA. Translation: CAI12792.1.
AL354950 Genomic DNA. Translation: CAI12793.1.
CH471066 Genomic DNA. Translation: EAW49160.1.
BC126130 mRNA. Translation: AAI26131.1.
BC130479 mRNA. Translation: AAI30480.1.

IPI

IPI00024242.
IPI00220430.

RefSeq

NP_001005463.1. NM_001005463.2.

UniGene

Hs.591374.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3MUJ	X-ray	1.92	A/B	261-395	[»]
3N50	X-ray	3.10	A/B/C/D/E/F	261-415	[»]

ProteinModelPortal

Q9H4W6.

SMR

Q9H4W6. Positions 35-414.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9H4W6.

Polymorphism databases

DMDM

13959320.

Proteomic databases

PRIDE

Q9H4W6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000355311; ENSP00000347463; ENSG00000108001.

GeneID

253738.

KEGG

hsa:253738.

UCSC

uc001lki.1. human.

Organism-specific databases

CTD

253738.

GeneCards

GC10M131633.

HGNC

HGNC:19087. EBF3.

MIM

607407. gene.

neXtProt

NX_Q9H4W6.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00390000014051.

HOGENOM

HBG444229.

HOVERGEN

HBG005108.

InParanoid

Q9H4W6.

OMA

ANSPYGM.

OrthoDB

EOG4TXBRK.

PhylomeDB

Q9H4W6.

Gene expression databases

ArrayExpress

Q9H4W6.

Bgee

Q9H4W6.

CleanEx

HS_EBF3.

Genevestigator

Q9H4W6.

GermOnline

ENSG00000108001. Homo sapiens.

Family and domain databases

InterPro

IPR011598. HLH_DNA-bd.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT_TIG_rcpt.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

KO

K09103.

PANTHER

PTHR10747. TF_COE. 1 hit.

Pfam

PF01833. TIG. 1 hit.
[Graphical view]

SMART

SM00353. HLH. 1 hit.
SM00429. IPT. 1 hit.
[Graphical view]

SUPFAM

SSF81296. Ig_E-set. 1 hit.

PROSITE

PS01345. COE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

COE3_HUMAN

Accession

Primary (citable) accession number: Q9H4W6
Secondary accession number(s): A0AUY1, Q5T6H9, Q9H4W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 4, 2001
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.