Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase NRDP1

Gene

RNF41

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88. Negatively regulates MYD88-dependent production of proinflammatory cytokines. Can promote TRIF-dependent production of type I interferon and inhibits infection with vesicular stomatitis virus (By similarity). Promotes also activation of TBK1 and IRF3. Involved in the ubiquitination of erythropoietin (EPO) and interleukin-3 (IL-3) receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages (By similarity). Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of intracellular redox state. The RNF41-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control (PubMed:24949970).By similarity7 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-type; degeneratePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri78 – 138SIAH-type; degeneratePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

  • erythropoietin receptor binding Source: Ensembl
  • interleukin-3 receptor binding Source: Ensembl
  • protein domain specific binding Source: CAFA
  • Ral GTPase binding Source: UniProtKB
  • receptor tyrosine kinase binding Source: CAFA
  • ubiquitin protein ligase activity Source: ParkinsonsUK-UCL
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • extrinsic apoptotic signaling pathway Source: UniProtKB
  • negative regulation of cell migration Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of mitophagy Source: Ensembl
  • positive regulation of DNA binding transcription factor activity Source: Ensembl
  • positive regulation of protein catabolic process Source: CAFA
  • positive regulation of reactive oxygen species metabolic process Source: ParkinsonsUK-UCL
  • proteasomal protein catabolic process Source: ParkinsonsUK-UCL
  • protein autoubiquitination Source: FlyBase
  • protein polyubiquitination Source: UniProtKB
  • regulation of establishment of cell polarity Source: Ensembl
  • regulation of lymphocyte differentiation Source: Ensembl
  • regulation of MAPK cascade Source: MGI
  • regulation of myeloid cell differentiation Source: Ensembl
  • regulation of protein kinase B signaling Source: MGI
  • regulation of reactive oxygen species metabolic process Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processApoptosis, Autophagy, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi6.3.2.19 2681
ReactomeiR-HSA-1358803 Downregulation of ERBB2:ERBB3 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase NRDP1 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 41
RING-type E3 ubiquitin transferase NRDP1Curated
Gene namesi
Name:RNF41
Synonyms:FLRF, NRDP1
ORF Names:SBBI03
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000181852.17
HGNCiHGNC:18401 RNF41
neXtProtiNX_Q9H4P4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi34C → S: Loss of activity; when associated with Q-36. 2 Publications1
Mutagenesisi36H → Q: Loss of activity; when associated with S-34. 2 Publications1
Mutagenesisi56D → V: Loss of activity. 1 Publication1

Organism-specific databases

DisGeNETi10193
OpenTargetsiENSG00000181852
PharmGKBiPA134875033

Polymorphism and mutation databases

BioMutaiRNF41
DMDMi88909120

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002239541 – 317E3 ubiquitin-protein ligase NRDP1Add BLAST317

Post-translational modificationi

Autoubiquitinated. Autoubiquitination leads to proteasomal degradation. Deubiquitinated by USP8 to get stabilized which induces apoptosis.3 Publications

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ9H4P4
MaxQBiQ9H4P4
PaxDbiQ9H4P4
PeptideAtlasiQ9H4P4
PRIDEiQ9H4P4

PTM databases

iPTMnetiQ9H4P4
PhosphoSitePlusiQ9H4P4

Expressioni

Tissue specificityi

Detected in ovary, testis and prostate.1 Publication

Gene expression databases

BgeeiENSG00000181852
CleanExiHS_RNF41
ExpressionAtlasiQ9H4P4 baseline and differential
GenevisibleiQ9H4P4 HS

Organism-specific databases

HPAiHPA016812

Interactioni

Subunit structurei

Interacts with USP8, ERBB3, PRKN and BIRC6. Interacts with CSF2RB, EPOR, IL3RA, MYD88 and TBK1. Interacts with CLEC16A (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • erythropoietin receptor binding Source: Ensembl
  • interleukin-3 receptor binding Source: Ensembl
  • protein domain specific binding Source: CAFA
  • Ral GTPase binding Source: UniProtKB
  • receptor tyrosine kinase binding Source: CAFA

Protein-protein interaction databases

BioGridi115488, 89 interactors
IntActiQ9H4P4, 53 interactors
MINTiQ9H4P4
STRINGi9606.ENSP00000342755

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi193 – 205Combined sources13
Beta strandi206 – 211Combined sources6
Helixi214 – 216Combined sources3
Beta strandi217 – 220Combined sources4
Helixi223 – 235Combined sources13
Helixi240 – 248Combined sources9
Helixi252 – 254Combined sources3
Turni257 – 259Combined sources3
Helixi262 – 267Combined sources6
Turni268 – 270Combined sources3
Helixi271 – 274Combined sources4
Beta strandi277 – 279Combined sources3
Beta strandi283 – 289Combined sources7
Helixi290 – 292Combined sources3
Turni298 – 300Combined sources3
Beta strandi303 – 312Combined sources10
Beta strandi314 – 316Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FZPX-ray1.87A193-317[»]
2GWFX-ray2.30B/D/F193-317[»]
ProteinModelPortaliQ9H4P4
SMRiQ9H4P4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H4P4

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-type; degeneratePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri78 – 138SIAH-type; degeneratePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410ISDX Eukaryota
ENOG410XNN7 LUCA
GeneTreeiENSGT00530000063647
HOGENOMiHOG000006561
HOVERGENiHBG053154
InParanoidiQ9H4P4
KOiK11981
OMAiSRQPTCP
OrthoDBiEOG091G0BKR
PhylomeDBiQ9H4P4
TreeFamiTF351947

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR015036 NRDP1
IPR037255 NRDP1_C
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
IPR013010 Znf_SIAH
PfamiView protein in Pfam
PF08941 USP8_interact, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SUPFAMiSSF160088 SSF160088, 1 hit
SSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H4P4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGYDVTRFQG DVDEDLICPI CSGVLEEPVQ APHCEHAFCN ACITQWFSQQ
60 70 80 90 100
QTCPVDRSVV TVAHLRPVPR IMRNMLSKLQ IACDNAVFGC SAVVRLDNLM
110 120 130 140 150
SHLSDCEHNP KRPVTCEQGC GLEMPKDELP NHNCIKHLRS VVQQQQTRIA
160 170 180 190 200
ELEKTSAEHK HQLAEQKRDI QLLKAYMRAI RSVNPNLQNL EETIEYNEIL
210 220 230 240 250
EWVNSLQPAR VTRWGGMIST PDAVLQAVIK RSLVESGCPA SIVNELIENA
260 270 280 290 300
HERSWPQGLA TLETRQMNRR YYENYVAKRI PGKQAVVVMA CENQHMGDDM
310
VQEPGLVMIF AHGVEEI
Length:317
Mass (Da):35,905
Last modified:February 21, 2006 - v2
Checksum:i46AE87AF8BE1A369
GO
Isoform 2 (identifier: Q9H4P4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-71: Missing.

Note: No experimental confirmation available.
Show »
Length:246
Mass (Da):27,965
Checksum:iF21D7FD03FA53642
GO

Sequence cautioni

The sequence AAG01988 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0446701 – 71Missing in isoform 2. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077599 mRNA Translation: AAC27647.1
AK314811 mRNA Translation: BAG37335.1
AC073896 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW96909.1
CH471054 Genomic DNA Translation: EAW96910.1
BC024284 mRNA No translation available.
BC032637 mRNA Translation: AAH32637.1
AY007109 mRNA Translation: AAG01988.1 Different initiation.
CCDSiCCDS8909.1 [Q9H4P4-1]
CCDS8910.1 [Q9H4P4-2]
RefSeqiNP_001229755.1, NM_001242826.1 [Q9H4P4-1]
NP_005776.1, NM_005785.3 [Q9H4P4-1]
NP_919339.1, NM_194358.2 [Q9H4P4-2]
NP_919340.1, NM_194359.2 [Q9H4P4-1]
XP_005268618.1, XM_005268561.4 [Q9H4P4-2]
XP_011536036.1, XM_011537734.2 [Q9H4P4-2]
XP_011536037.1, XM_011537735.2 [Q9H4P4-2]
UniGeneiHs.524502

Genome annotation databases

EnsembliENST00000345093; ENSP00000342755; ENSG00000181852 [Q9H4P4-1]
ENST00000394013; ENSP00000377581; ENSG00000181852 [Q9H4P4-2]
ENST00000552656; ENSP00000447303; ENSG00000181852 [Q9H4P4-1]
ENST00000615206; ENSP00000484671; ENSG00000181852 [Q9H4P4-1]
GeneIDi10193
KEGGihsa:10193
UCSCiuc001ske.3 human [Q9H4P4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRNF41_HUMAN
AccessioniPrimary (citable) accession number: Q9H4P4
Secondary accession number(s): A6NFW0, B2RBT8, O75598
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: February 21, 2006
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health