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Protein

Sentrin-specific protease 3

Gene

SENP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.7 Publications

Catalytic activityi

Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.

Enzyme regulationi

On oxidative stress, SENP3 degradation is blocked by inhibition of its ubiquitination, which stabilizes it as it accumulates in the nucleoplasm.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei465By similarity1
Active sitei482By similarity1
Active sitei532By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000161956-MONOMER.
BRENDAi3.4.22.B72. 2681.
ReactomeiR-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Protein family/group databases

MEROPSiC48.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Sentrin-specific protease 3 (EC:3.4.22.68)
Alternative name(s):
SUMO-1-specific protease 3
Sentrin/SUMO-specific protease SENP3
Gene namesi
Name:SENP3
Synonyms:SSP3, SUSP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:17862. SENP3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • MLL1 complex Source: UniProtKB
  • nucleolus Source: LIFEdb
  • nucleoplasm Source: Reactome
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi532C → S: Loss of enzymatic activity. 1 Publication1

Organism-specific databases

DisGeNETi26168.
OpenTargetsiENSG00000161956.
PharmGKBiPA134933213.

Polymorphism and mutation databases

BioMutaiSENP3.
DMDMi119370525.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001017211 – 574Sentrin-specific protease 3Add BLAST574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54PhosphoserineCombined sources1
Modified residuei73PhosphoserineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei169PhosphoserineBy similarity1
Modified residuei176PhosphothreonineBy similarity1
Modified residuei181PhosphoserineBy similarity1
Modified residuei188PhosphoserineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei232PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9H4L4.
MaxQBiQ9H4L4.
PaxDbiQ9H4L4.
PeptideAtlasiQ9H4L4.
PRIDEiQ9H4L4.

PTM databases

iPTMnetiQ9H4L4.
PhosphoSitePlusiQ9H4L4.

Expressioni

Gene expression databases

BgeeiENSG00000161956.
CleanExiHS_SENP3.
ExpressionAtlasiQ9H4L4. baseline and differential.
GenevisibleiQ9H4L4. HS.

Organism-specific databases

HPAiHPA060290.

Interactioni

Subunit structurei

Binds to SUMO1 and SUMO3 (By similarity). Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with EP300, NPM1 and CDCA8. Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148. Interacts with NOL9. Interacts with CCAR2.By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NPM1P067486EBI-2880236,EBI-78579
PELP1Q8IZL85EBI-2880236,EBI-716449
TEX10Q9NXF12EBI-2880236,EBI-2371062
UBCP0CG482EBI-2880236,EBI-3390054
WDR18Q9BV383EBI-2880236,EBI-727429

Protein-protein interaction databases

BioGridi117594. 116 interactors.
DIPiDIP-47511N.
IntActiQ9H4L4. 29 interactors.
MINTiMINT-1200849.
STRINGi9606.ENSP00000403712.

Structurei

3D structure databases

ProteinModelPortaliQ9H4L4.
SMRiQ9H4L4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni386 – 543ProteaseAdd BLAST158

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi125 – 128Nuclear localization signalSequence analysis4
Motifi153 – 159Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 68Pro-richAdd BLAST56
Compositional biasi76 – 92Glu-richAdd BLAST17
Compositional biasi192 – 195Poly-Pro4

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGiKOG0778. Eukaryota.
COG5160. LUCA.
GeneTreeiENSGT00530000062941.
HOGENOMiHOG000154287.
HOVERGENiHBG059450.
InParanoidiQ9H4L4.
KOiK08593.
OrthoDBiEOG091G092N.
PhylomeDBiQ9H4L4.
TreeFamiTF316289.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H4L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKETIQGTGS WGPEPPGPGI PPAYSSPRRE RLRWPPPPKP RLKSGGGFGP
60 70 80 90 100
DPGSGTTVPA RRLPVPRPSF DASASEEEEE EEEEEDEDEE EEVAAWRLPP
110 120 130 140 150
RWSQLGTSQR PRPSRPTHRK TCSQRRRRAM RAFRMLLYSK STSLTFHWKL
160 170 180 190 200
WGRHRGRRRG LAHPKNHLSP QQGGATPQVP SPCCRFDSPR GPPPPRLGLL
210 220 230 240 250
GALMAEDGVR GSPPVPSGPP MEEDGLRWTP KSPLDPDSGL LSCTLPNGFG
260 270 280 290 300
GQSGPEGERS LAPPDASILI SNVCSIGDHV AQELFQGSDL GMAEEAERPG
310 320 330 340 350
EKAGQHSPLR EEHVTCVQSI LDEFLQTYGS LIPLSTDEVV EKLEDIFQQE
360 370 380 390 400
FSTPSRKGLV LQLIQSYQRM PGNAMVRGFR VAYKRHVLTM DDLGTLYGQN
410 420 430 440 450
WLNDQVMNMY GDLVMDTVPE KVHFFNSFFY DKLRTKGYDG VKRWTKNVDI
460 470 480 490 500
FNKELLLIPI HLEVHWSLIS VDVRRRTITY FDSQRTLNRR CPKHIAKYLQ
510 520 530 540 550
AEAVKKDRLD FHQGWKGYFK MNVARQNNDS DCGAFVLQYC KHLALSQPFS
560 570
FTQQDMPKLR RQIYKELCHC KLTV
Length:574
Mass (Da):65,010
Last modified:December 12, 2006 - v2
Checksum:iE495137EE7500741
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30 – 31ER → DG in AAG33252 (PubMed:10806345).Curated2
Sequence conflicti60A → T in AAG33252 (PubMed:10806345).Curated1
Sequence conflicti65V → A in AAG33252 (PubMed:10806345).Curated1
Sequence conflicti113 – 114PS → AL in AAG33252 (PubMed:10806345).Curated2
Sequence conflicti117T → S in AAG33252 (PubMed:10806345).Curated1
Sequence conflicti134R → Q in AAG33252 (PubMed:10806345).Curated1
Sequence conflicti161L → S in AAH48306 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051544515W → R.Corresponds to variant rs9972914dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008763 mRNA. Translation: AAG33252.1.
AF199459 mRNA. Translation: AAL25652.1.
AL050283 mRNA. Translation: CAB43384.2.
AL834294 mRNA. Translation: CAD38967.1.
BC048306 mRNA. Translation: AAH48306.1.
BC080658 mRNA. Translation: AAH80658.1.
CCDSiCCDS73958.1.
PIRiT08759.
RefSeqiNP_056485.2. NM_015670.5.
UniGeneiHs.513926.
Hs.733104.

Genome annotation databases

EnsembliENST00000321337; ENSP00000314029; ENSG00000161956.
ENST00000429205; ENSP00000403712; ENSG00000161956.
GeneIDi26168.
KEGGihsa:26168.
UCSCiuc032esp.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008763 mRNA. Translation: AAG33252.1.
AF199459 mRNA. Translation: AAL25652.1.
AL050283 mRNA. Translation: CAB43384.2.
AL834294 mRNA. Translation: CAD38967.1.
BC048306 mRNA. Translation: AAH48306.1.
BC080658 mRNA. Translation: AAH80658.1.
CCDSiCCDS73958.1.
PIRiT08759.
RefSeqiNP_056485.2. NM_015670.5.
UniGeneiHs.513926.
Hs.733104.

3D structure databases

ProteinModelPortaliQ9H4L4.
SMRiQ9H4L4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117594. 116 interactors.
DIPiDIP-47511N.
IntActiQ9H4L4. 29 interactors.
MINTiMINT-1200849.
STRINGi9606.ENSP00000403712.

Protein family/group databases

MEROPSiC48.003.

PTM databases

iPTMnetiQ9H4L4.
PhosphoSitePlusiQ9H4L4.

Polymorphism and mutation databases

BioMutaiSENP3.
DMDMi119370525.

Proteomic databases

EPDiQ9H4L4.
MaxQBiQ9H4L4.
PaxDbiQ9H4L4.
PeptideAtlasiQ9H4L4.
PRIDEiQ9H4L4.

Protocols and materials databases

DNASUi26168.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000321337; ENSP00000314029; ENSG00000161956.
ENST00000429205; ENSP00000403712; ENSG00000161956.
GeneIDi26168.
KEGGihsa:26168.
UCSCiuc032esp.2. human.

Organism-specific databases

CTDi26168.
DisGeNETi26168.
GeneCardsiSENP3.
H-InvDBHIX0013501.
HGNCiHGNC:17862. SENP3.
HPAiHPA060290.
MIMi612844. gene.
neXtProtiNX_Q9H4L4.
OpenTargetsiENSG00000161956.
PharmGKBiPA134933213.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0778. Eukaryota.
COG5160. LUCA.
GeneTreeiENSGT00530000062941.
HOGENOMiHOG000154287.
HOVERGENiHBG059450.
InParanoidiQ9H4L4.
KOiK08593.
OrthoDBiEOG091G092N.
PhylomeDBiQ9H4L4.
TreeFamiTF316289.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000161956-MONOMER.
BRENDAi3.4.22.B72. 2681.
ReactomeiR-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

ChiTaRSiSENP3. human.
GeneWikiiSENP3.
GenomeRNAii26168.
PROiQ9H4L4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161956.
CleanExiHS_SENP3.
ExpressionAtlasiQ9H4L4. baseline and differential.
GenevisibleiQ9H4L4. HS.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSENP3_HUMAN
AccessioniPrimary (citable) accession number: Q9H4L4
Secondary accession number(s): Q66K15
, Q86VS7, Q96PS4, Q9Y3W9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: December 12, 2006
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.