Q9H4G4 (GAPR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi-associated plant pathogenesis-related protein 1
Short name=GAPR-1
Short name=Golgi-associated PR-1 protein

Alternative name(s):
Glioma pathogenesis-related protein 2
Short name=GliPR 2

Gene names

Name:	GLIPR2
Synonyms:	C9orf19, GAPR1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Homodimer. Interacts with CAV1. Ref.1 Ref.6
Subcellular location	Golgi apparatus membrane; Lipid-anchor. Note: Binds lipid-enriched microdomains of Golgi membranes not only by ionic interactions but also through the myristate. Ref.1
Tissue specificity	Highest expression in lung and peripheral leukocytes, and minor expression in liver and kidney. Ref.2
Sequence similarities	Belongs to the CRISP family. Contains 1 SCP domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil
PTM	Lipoprotein Myristate
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 154

153

Golgi-associated plant pathogenesis-related protein 1

PRO_0000211524

Regions

Domain

14 – 132

119

SCP

Region

91 – 98

8

Interaction with CAV1

Coiled coil

30 – 53

24

Potential

Amino acid modifications

Lipidation

2

1

N-myristoyl glycine Ref.1

Experimental info

Sequence conflict

37

1

R → Q AA sequence Ref.1

Sequence conflict

87 – 90

4

IKNY → VHFM in AAH17918. Ref.5

Sequence conflict

101 – 154

54

TGHFT…LPPKK → IRFFFFNFLLFLSKPLLYFS YF in BAC11019. Ref.3

Secondary structure

1

.

.

.

.

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.

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.

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154

Helix Strand Turn

Details...

Helix

5 – 23

19

Helix

33 – 49

17

Helix

56 – 59

4

Beta strand

62 – 72

11

Helix

76 – 84

9

Helix

85 – 89

5

Turn

99 – 101

3

Helix

102 – 108

7

Beta strand

114 – 121

8

Beta strand

127 – 135

9

Helix

144 – 148

5

Sequences

	Sequence		Length	Mass (Da)
	Q9H4G4 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5AA9B26A21EF1039	FASTA	154	17,218
	10 20 30 40 50 60 MGKSASKQFH NEVLKAHNEY RQKHGVPPLK LCKNLNREAQ QYSEALASTR ILKHSPESSR 70 80 90 100 110 120 GQCGENLAWA SYDQTGKEVA DRWYSEIKNY NFQQPGFTSG TGHFTAMVWK NTKKMGVGKA 130 140 150 SASDGSSFVV ARYFPAGNVV NEGFFEENVL PPKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex." Eberle H.B., Serrano R.L., Fuellekrug J., Schlosser A., Lehmann W.D., Lottspeich F., Kaloyanova D., Wieland F.T., Helms J.B. J. Cell Sci. 115:827-838(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-49 AND 54-68, SUBCELLULAR LOCATION, MYRISTOYLATION AT GLY-2, INTERACTION WITH CAV1. Tissue: Brain.
[2]	"Cloning and characterization of a human novel gene C9orf19 encoding a conserved putative protein with an SCP-like extracellular protein domain." Eisenberg I., Barash M., Kahan T., Mitrani-Rosenbaum S. Gene 293:141-148(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Placenta.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo and Placenta.
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-90. Tissue: Prostate.
[6]	"Structural analysis of the human Golgi-associated plant pathogenesis related protein GAPR-1 implicates dimerization as a regulatory mechanism." Serrano R.L., Kuhn A., Hendricks A., Helms J.B., Sinning I., Groves M.R. J. Mol. Biol. 339:173-183(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS), HOMODIMERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ011129 mRNA. Translation: CAC12812.1.
AY039756 mRNA. Translation: AAK83460.1.
AK074488 mRNA. Translation: BAC11019.1.
AK075091 mRNA. Translation: BAC11395.1.
AL158830 Genomic DNA. Translation: CAH73678.1.
BC017918 mRNA. Translation: AAH17918.1.

IPI

IPI00007067.

RefSeq

NP_071738.1. NM_022343.2.

UniGene

Hs.493819.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SMB	X-ray	1.55	A	1-154	[»]
4AIW	X-ray	1.50	A	1-154	[»]

ProteinModelPortal

Q9H4G4.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000367196.

PTM databases

PhosphoSite

Q9H4G4.

Polymorphism databases

DMDM

46397431.

Proteomic databases

PaxDb

Q9H4G4.

PeptideAtlas

Q9H4G4.

PRIDE

Q9H4G4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000377960; ENSP00000367196; ENSG00000122694.

GeneID

152007.

KEGG

hsa:152007.

UCSC

uc003zyy.1. human.

Organism-specific databases

CTD

152007.

GeneCards

GC09P036127.

HGNC

HGNC:18007. GLIPR2.

HPA

HPA029478.

MIM

607141. gene.

neXtProt

NX_Q9H4G4.

PharmGKB

PA164720283.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2340.

HOVERGEN

HBG051701.

OMA

DQSGKDV.

PhylomeDB

Q9H4G4.

Gene expression databases

ArrayExpress

Q9H4G4.

Bgee

Q9H4G4.

CleanEx

HS_GLIPR2.

Genevestigator

Q9H4G4.

GermOnline

ENSG00000122694. Homo sapiens.

Family and domain databases

Gene3D

3.40.33.10. 1 hit.

InterPro

IPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
[Graphical view]

PANTHER

PTHR10334. PTHR10334. 1 hit.

Pfam

PF00188. CAP. 1 hit.
[Graphical view]

PRINTS

PR00837. V5TPXLIKE.

SMART

SM00198. SCP. 1 hit.
[Graphical view]

SUPFAM

SSF55797. SCP-like_extracellular. 1 hit.

PROSITE

PS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. False negative.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

GLIPR2. human.

EvolutionaryTrace

Q9H4G4.

GenomeRNAi

152007.

NextBio

86851.

SOURCE

Search...

Entry information

Entry name

GAPR1_HUMAN

Accession

Primary (citable) accession number: Q9H4G4
Secondary accession number(s): Q5VZR1 , Q8N2S6, Q8WWC9, Q8WX36

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.