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Q9H4E5

- RHOJ_HUMAN

UniProt

Q9H4E5 - RHOJ_HUMAN

Protein

Rho-related GTP-binding protein RhoJ

Gene

RHOJ

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 358GTPBy similarity
    Nucleotide bindingi75 – 795GTPBy similarity
    Nucleotide bindingi133 – 1364GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB
    2. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. actin cytoskeleton organization Source: UniProtKB
    2. GTP catabolic process Source: GOC
    3. regulation of cell shape Source: UniProtKB-KW
    4. regulation of small GTPase mediated signal transduction Source: Reactome
    5. retina vasculature morphogenesis in camera-type eye Source: Ensembl
    6. Rho protein signal transduction Source: Ensembl
    7. small GTPase mediated signal transduction Source: Reactome

    Keywords - Biological processi

    Cell shape

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_11051. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho-related GTP-binding protein RhoJ
    Alternative name(s):
    Ras-like protein family member 7B
    Tc10-like GTP-binding protein
    Gene namesi
    Name:RHOJ
    Synonyms:ARHJ, RASL7B, RHOI, TCL
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:688. RHOJ.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24981.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 211211Rho-related GTP-binding protein RhoJPRO_0000198869Add
    BLAST
    Propeptidei212 – 2143Removed in mature formBy similarityPRO_0000281220

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei211 – 2111Cysteine methyl esterBy similarity
    Lipidationi211 – 2111S-farnesyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiQ9H4E5.
    PaxDbiQ9H4E5.
    PRIDEiQ9H4E5.

    PTM databases

    PhosphoSiteiQ9H4E5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9H4E5.
    BgeeiQ9H4E5.
    CleanExiHS_RHOJ.
    GenevestigatoriQ9H4E5.

    Organism-specific databases

    HPAiHPA003050.

    Interactioni

    Subunit structurei

    Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp.By similarity

    Protein-protein interaction databases

    BioGridi121499. 2 interactions.
    IntActiQ9H4E5. 2 interactions.
    STRINGi9606.ENSP00000316729.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H4E5.
    SMRiQ9H4E5. Positions 21-197.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 589Effector regionSequence Analysis

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rho family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOVERGENiHBG009351.
    InParanoidiQ9H4E5.
    KOiK07864.
    OMAiARMNCKE.
    PhylomeDBiQ9H4E5.
    TreeFamiTF101109.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00174. RHO. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51420. RHO. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9H4E5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNCKEGTDSS CGCRGNDEKK MLKCVVVGDG AVGKTCLLMS YANDAFPEEY    50
    VPTVFDHYAV TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF 100
    SVVNPASYHN VQEEWVPELK DCMPHVPYVL IGTQIDLRDD PKTLARLLYM 150
    KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ KGLKAVFDEA ILTIFHPKKK 200
    KKRCSEGHSC CSII 214
    Length:214
    Mass (Da):23,821
    Last modified:March 1, 2001 - v1
    Checksum:iAAD2E2CED036F48C
    GO
    Isoform 2 (identifier: Q9H4E5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         113-134: Missing.

    Note: No experimental confirmation available. Could be created by usage of an unusual splicing donor site.

    Show »
    Length:192
    Mass (Da):21,255
    Checksum:iF20844F6EC738489
    GO

    Sequence cautioni

    The sequence BAB55013.1 differs from that shown. Reason: Frameshift at position 143.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31C → R in BAB55013. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei113 – 13422Missing in isoform 2. 1 PublicationVSP_007231Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ276567 mRNA. Translation: CAC06611.1.
    AF309563 mRNA. Translation: AAL09440.1.
    AK027278 mRNA. Translation: BAB55013.1. Frameshift.
    AK027351 mRNA. Translation: BAB55055.1.
    AF498977 mRNA. Translation: AAM21124.1.
    BC062575 mRNA. Translation: AAH62575.1.
    CCDSiCCDS9757.1. [Q9H4E5-1]
    RefSeqiNP_065714.1. NM_020663.4. [Q9H4E5-1]
    UniGeneiHs.656339.

    Genome annotation databases

    EnsembliENST00000316754; ENSP00000316729; ENSG00000126785. [Q9H4E5-1]
    GeneIDi57381.
    KEGGihsa:57381.
    UCSCiuc001xgb.2. human. [Q9H4E5-1]

    Polymorphism databases

    DMDMi24418646.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ276567 mRNA. Translation: CAC06611.1 .
    AF309563 mRNA. Translation: AAL09440.1 .
    AK027278 mRNA. Translation: BAB55013.1 . Frameshift.
    AK027351 mRNA. Translation: BAB55055.1 .
    AF498977 mRNA. Translation: AAM21124.1 .
    BC062575 mRNA. Translation: AAH62575.1 .
    CCDSi CCDS9757.1. [Q9H4E5-1 ]
    RefSeqi NP_065714.1. NM_020663.4. [Q9H4E5-1 ]
    UniGenei Hs.656339.

    3D structure databases

    ProteinModelPortali Q9H4E5.
    SMRi Q9H4E5. Positions 21-197.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121499. 2 interactions.
    IntActi Q9H4E5. 2 interactions.
    STRINGi 9606.ENSP00000316729.

    PTM databases

    PhosphoSitei Q9H4E5.

    Polymorphism databases

    DMDMi 24418646.

    Proteomic databases

    MaxQBi Q9H4E5.
    PaxDbi Q9H4E5.
    PRIDEi Q9H4E5.

    Protocols and materials databases

    DNASUi 57381.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000316754 ; ENSP00000316729 ; ENSG00000126785 . [Q9H4E5-1 ]
    GeneIDi 57381.
    KEGGi hsa:57381.
    UCSCi uc001xgb.2. human. [Q9H4E5-1 ]

    Organism-specific databases

    CTDi 57381.
    GeneCardsi GC14P063671.
    HGNCi HGNC:688. RHOJ.
    HPAi HPA003050.
    MIMi 607653. gene.
    neXtProti NX_Q9H4E5.
    PharmGKBi PA24981.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOVERGENi HBG009351.
    InParanoidi Q9H4E5.
    KOi K07864.
    OMAi ARMNCKE.
    PhylomeDBi Q9H4E5.
    TreeFami TF101109.

    Enzyme and pathway databases

    Reactomei REACT_11051. Rho GTPase cycle.

    Miscellaneous databases

    ChiTaRSi RHOJ. human.
    GeneWikii RHOJ.
    GenomeRNAii 57381.
    NextBioi 63486.
    PROi Q9H4E5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9H4E5.
    Bgeei Q9H4E5.
    CleanExi HS_RHOJ.
    Genevestigatori Q9H4E5.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00174. RHO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51420. RHO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of TCL, a new GTPase of the Rho family related to TC10 and Cdc42."
      Vignal E., De Toledo M., Comunale F., Ladopoulou A., Gauthier-Rouviere C., Blangy A., Fort P.
      J. Biol. Chem. 275:36457-36464(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "A novel Rho GTPase (RhoI) induces loss of stress-fibers and results in apical actin reorganization."
      Allen M., Halford S., Daniels H., McIntosh B., Kanuga N., Greenwood J., Carey A.H., Adamson P.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Embryo.
    4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Colon.

    Entry informationi

    Entry nameiRHOJ_HUMAN
    AccessioniPrimary (citable) accession number: Q9H4E5
    Secondary accession number(s): Q96KC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3