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Q9H4E5

- RHOJ_HUMAN

UniProt

Q9H4E5 - RHOJ_HUMAN

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Protein

Rho-related GTP-binding protein RhoJ

Gene

RHOJ

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 358GTPBy similarity
Nucleotide bindingi75 – 795GTPBy similarity
Nucleotide bindingi133 – 1364GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. actin cytoskeleton organization Source: UniProtKB
  2. GTP catabolic process Source: GOC
  3. regulation of cell shape Source: UniProtKB-KW
  4. regulation of small GTPase mediated signal transduction Source: Reactome
  5. retina vasculature morphogenesis in camera-type eye Source: Ensembl
  6. Rho protein signal transduction Source: Ensembl
  7. small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell shape

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoJ
Alternative name(s):
Ras-like protein family member 7B
Tc10-like GTP-binding protein
Gene namesi
Name:RHOJ
Synonyms:ARHJ, RASL7B, RHOI, TCL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:688. RHOJ.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. extracellular vesicular exosome Source: UniProtKB
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24981.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Rho-related GTP-binding protein RhoJPRO_0000198869Add
BLAST
Propeptidei212 – 2143Removed in mature formBy similarityPRO_0000281220

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei211 – 2111Cysteine methyl esterBy similarity
Lipidationi211 – 2111S-farnesyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiQ9H4E5.
PaxDbiQ9H4E5.
PRIDEiQ9H4E5.

PTM databases

PhosphoSiteiQ9H4E5.

Expressioni

Gene expression databases

BgeeiQ9H4E5.
CleanExiHS_RHOJ.
ExpressionAtlasiQ9H4E5. baseline and differential.
GenevestigatoriQ9H4E5.

Organism-specific databases

HPAiHPA003050.

Interactioni

Subunit structurei

Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp.By similarity

Protein-protein interaction databases

BioGridi121499. 10 interactions.
IntActiQ9H4E5. 2 interactions.
STRINGi9606.ENSP00000316729.

Structurei

3D structure databases

ProteinModelPortaliQ9H4E5.
SMRiQ9H4E5. Positions 21-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 589Effector regionSequence Analysis

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118978.
HOVERGENiHBG009351.
InParanoidiQ9H4E5.
KOiK07864.
OMAiARMNCKE.
PhylomeDBiQ9H4E5.
TreeFamiTF101109.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9H4E5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNCKEGTDSS CGCRGNDEKK MLKCVVVGDG AVGKTCLLMS YANDAFPEEY
60 70 80 90 100
VPTVFDHYAV TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF
110 120 130 140 150
SVVNPASYHN VQEEWVPELK DCMPHVPYVL IGTQIDLRDD PKTLARLLYM
160 170 180 190 200
KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ KGLKAVFDEA ILTIFHPKKK
210
KKRCSEGHSC CSII
Length:214
Mass (Da):23,821
Last modified:March 1, 2001 - v1
Checksum:iAAD2E2CED036F48C
GO
Isoform 2 (identifier: Q9H4E5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-134: Missing.

Note: No experimental confirmation available. Could be created by usage of an unusual splicing donor site.

Show »
Length:192
Mass (Da):21,255
Checksum:iF20844F6EC738489
GO

Sequence cautioni

The sequence BAB55013.1 differs from that shown. Reason: Frameshift at position 143. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31C → R in BAB55013. (PubMed:14702039)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei113 – 13422Missing in isoform 2. 1 PublicationVSP_007231Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276567 mRNA. Translation: CAC06611.1.
AF309563 mRNA. Translation: AAL09440.1.
AK027278 mRNA. Translation: BAB55013.1. Frameshift.
AK027351 mRNA. Translation: BAB55055.1.
AF498977 mRNA. Translation: AAM21124.1.
BC062575 mRNA. Translation: AAH62575.1.
CCDSiCCDS9757.1. [Q9H4E5-1]
RefSeqiNP_065714.1. NM_020663.4. [Q9H4E5-1]
UniGeneiHs.656339.

Genome annotation databases

EnsembliENST00000316754; ENSP00000316729; ENSG00000126785. [Q9H4E5-1]
GeneIDi57381.
KEGGihsa:57381.
UCSCiuc001xgb.2. human. [Q9H4E5-1]

Polymorphism databases

DMDMi24418646.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276567 mRNA. Translation: CAC06611.1 .
AF309563 mRNA. Translation: AAL09440.1 .
AK027278 mRNA. Translation: BAB55013.1 . Frameshift.
AK027351 mRNA. Translation: BAB55055.1 .
AF498977 mRNA. Translation: AAM21124.1 .
BC062575 mRNA. Translation: AAH62575.1 .
CCDSi CCDS9757.1. [Q9H4E5-1 ]
RefSeqi NP_065714.1. NM_020663.4. [Q9H4E5-1 ]
UniGenei Hs.656339.

3D structure databases

ProteinModelPortali Q9H4E5.
SMRi Q9H4E5. Positions 21-197.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121499. 10 interactions.
IntActi Q9H4E5. 2 interactions.
STRINGi 9606.ENSP00000316729.

PTM databases

PhosphoSitei Q9H4E5.

Polymorphism databases

DMDMi 24418646.

Proteomic databases

MaxQBi Q9H4E5.
PaxDbi Q9H4E5.
PRIDEi Q9H4E5.

Protocols and materials databases

DNASUi 57381.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000316754 ; ENSP00000316729 ; ENSG00000126785 . [Q9H4E5-1 ]
GeneIDi 57381.
KEGGi hsa:57381.
UCSCi uc001xgb.2. human. [Q9H4E5-1 ]

Organism-specific databases

CTDi 57381.
GeneCardsi GC14P063671.
HGNCi HGNC:688. RHOJ.
HPAi HPA003050.
MIMi 607653. gene.
neXtProti NX_Q9H4E5.
PharmGKBi PA24981.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000118978.
HOVERGENi HBG009351.
InParanoidi Q9H4E5.
KOi K07864.
OMAi ARMNCKE.
PhylomeDBi Q9H4E5.
TreeFami TF101109.

Enzyme and pathway databases

Reactomei REACT_11051. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSi RHOJ. human.
GeneWikii RHOJ.
GenomeRNAii 57381.
NextBioi 63486.
PROi Q9H4E5.
SOURCEi Search...

Gene expression databases

Bgeei Q9H4E5.
CleanExi HS_RHOJ.
ExpressionAtlasi Q9H4E5. baseline and differential.
Genevestigatori Q9H4E5.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00174. RHO. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51420. RHO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of TCL, a new GTPase of the Rho family related to TC10 and Cdc42."
    Vignal E., De Toledo M., Comunale F., Ladopoulou A., Gauthier-Rouviere C., Blangy A., Fort P.
    J. Biol. Chem. 275:36457-36464(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "A novel Rho GTPase (RhoI) induces loss of stress-fibers and results in apical actin reorganization."
    Allen M., Halford S., Daniels H., McIntosh B., Kanuga N., Greenwood J., Carey A.H., Adamson P.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Embryo.
  4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon.

Entry informationi

Entry nameiRHOJ_HUMAN
AccessioniPrimary (citable) accession number: Q9H4E5
Secondary accession number(s): Q96KC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3