Q9H4E5 (RHOJ_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho-related GTP-binding protein RhoJ

Alternative name(s):
Ras-like protein family member 7B
Tc10-like GTP-binding protein

Gene names

Name:	RHOJ
Synonyms:	ARHJ, RASL7B, RHOI, TCL

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology By similarity.
Subunit structure	Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.
Sequence caution	The sequence BAB55013.1 differs from that shown. Reason: Frameshift at position 143.

Ontologies

Keywords
Biological process	Cell shape
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Inferred from electronic annotation. Source: Compara actin cytoskeleton organization Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H4E5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H4E5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     113-134: Missing.

Note: No experimental confirmation available. Could be created by usage of an unusual splicing donor site.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Rho-related GTP-binding protein RhoJ		PRO_0000198869
	Propeptide	212 – 214	3	Removed in mature form By similarity		PRO_0000281220
Regions
	Nucleotide binding	28 – 35	8	GTP By similarity
	Nucleotide binding	75 – 79	5	GTP By similarity
	Nucleotide binding	133 – 136	4	GTP By similarity
	Motif	50 – 58	9	Effector region Potential
Amino acid modifications
	Modified residue	211	1	Cysteine methyl ester By similarity
	Lipidation	211	1	S-farnesyl cysteine By similarity
Natural variations
	Alternative sequence	113 – 134	22	Missing in isoform 2.		VSP_007231
Experimental info
	Sequence conflict	3	1	C → R in BAB55013. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: AAD2E2CED036F48C	FASTA	214	23,821
	10 20 30 40 50 60 MNCKEGTDSS CGCRGNDEKK MLKCVVVGDG AVGKTCLLMS YANDAFPEEY VPTVFDHYAV 70 80 90 100 110 120 TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF SVVNPASYHN VQEEWVPELK 130 140 150 160 170 180 DCMPHVPYVL IGTQIDLRDD PKTLARLLYM KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ 190 200 210 KGLKAVFDEA ILTIFHPKKK KKRCSEGHSC CSII
	Isoform 2 [UniParc]. Checksum: F20844F6EC738489	FASTA	192	21,255
	10 20 30 40 50 60 MNCKEGTDSS CGCRGNDEKK MLKCVVVGDG AVGKTCLLMS YANDAFPEEY VPTVFDHYAV 70 80 90 100 110 120 TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF SVVNPASYHN VQIDLRDDPK 130 140 150 160 170 180 TLARLLYMKE KPLTYEHGVK LAKAIGAQCY LECSALTQKG LKAVFDEAIL TIFHPKKKKK 190 RCSEGHSCCS II

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of TCL, a new GTPase of the Rho family related to TC10 and Cdc42." Vignal E., De Toledo M., Comunale F., Ladopoulou A., Gauthier-Rouviere C., Blangy A., Fort P. J. Biol. Chem. 275:36457-36464(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"A novel Rho GTPase (RhoI) induces loss of stress-fibers and results in apical actin reorganization." Allen M., Halford S., Daniels H., McIntosh B., Kanuga N., Greenwood J., Carey A.H., Adamson P. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Embryo.
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ276567 mRNA. Translation: CAC06611.1. AF309563 mRNA. Translation: AAL09440.1. AK027278 mRNA. Translation: BAB55013.1. Frameshift. AK027351 mRNA. Translation: BAB55055.1. AF498977 mRNA. Translation: AAM21124.1. BC062575 mRNA. Translation: AAH62575.1.
IPI	IPI00027952. IPI00249526.
RefSeq	NP_065714.1. NM_020663.4.
UniGene	Hs.656339.
3D structure databases
ProteinModelPortal	Q9H4E5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H4E5. 2 interactions.
STRING	9606.ENSP00000316729.
PTM databases
PhosphoSite	Q9H4E5.
Polymorphism databases
DMDM	24418646.
Proteomic databases
PaxDb	Q9H4E5.
PRIDE	Q9H4E5.
Protocols and materials databases
DNASU	57381.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000316754; ENSP00000316729; ENSG00000126785.
GeneID	57381.
KEGG	hsa:57381.
UCSC	uc001xgb.2. human.
Organism-specific databases
CTD	57381.
GeneCards	GC14P063671.
HGNC	HGNC:688. RHOJ.
HPA	HPA003050.
MIM	607653. gene.
neXtProt	NX_Q9H4E5.
PharmGKB	PA24981.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1100.
HOVERGEN	HBG009351.
InParanoid	Q9H4E5.
KO	K07864.
OMA	NDSSCGC.
OrthoDB	EOG434W6W.
PhylomeDB	Q9H4E5.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9H4E5.
Bgee	Q9H4E5.
CleanEx	HS_RHOJ.
Genevestigator	Q9H4E5.
GermOnline	ENSG00000126785. Homo sapiens.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RHOJ. human.
GenomeRNAi	57381.
NextBio	63486.
SOURCE	Search...

Entry information

Entry name

RHOJ_HUMAN

Accession

Primary (citable) accession number: Q9H4E5
Secondary accession number(s): Q96KC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.