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Protein

Rho-related GTP-binding protein RhoJ

Gene

RHOJ

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 35GTPBy similarity8
Nucleotide bindingi75 – 79GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Biological processCell shape
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoJ
Alternative name(s):
Ras-like protein family member 7B
Tc10-like GTP-binding protein
Gene namesi
Name:RHOJ
Synonyms:ARHJ, RASL7B, RHOI, TCL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:688. RHOJ.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • plasma membrane Source: Reactome

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi57381.
OpenTargetsiENSG00000126785.
PharmGKBiPA24981.

Polymorphism and mutation databases

BioMutaiRHOJ.
DMDMi24418646.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988691 – 211Rho-related GTP-binding protein RhoJAdd BLAST211
PropeptideiPRO_0000281220212 – 214Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei211Cysteine methyl esterBy similarity1
Lipidationi211S-farnesyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiQ9H4E5.
PaxDbiQ9H4E5.
PeptideAtlasiQ9H4E5.
PRIDEiQ9H4E5.

PTM databases

iPTMnetiQ9H4E5.
PhosphoSitePlusiQ9H4E5.
SwissPalmiQ9H4E5.

Expressioni

Gene expression databases

BgeeiENSG00000126785.
CleanExiHS_RHOJ.
ExpressionAtlasiQ9H4E5. baseline and differential.
GenevisibleiQ9H4E5. HS.

Organism-specific databases

HPAiHPA003050.

Interactioni

Subunit structurei

Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp.By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi121499. 20 interactors.
IntActiQ9H4E5. 17 interactors.
STRINGi9606.ENSP00000316729.

Structurei

3D structure databases

ProteinModelPortaliQ9H4E5.
SMRiQ9H4E5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 58Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOVERGENiHBG009351.
InParanoidiQ9H4E5.
KOiK07864.
OMAiKKRCSEC.
OrthoDBiEOG091G0KCM.
PhylomeDBiQ9H4E5.
TreeFamiTF101109.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51420. RHO. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H4E5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNCKEGTDSS CGCRGNDEKK MLKCVVVGDG AVGKTCLLMS YANDAFPEEY
60 70 80 90 100
VPTVFDHYAV TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF
110 120 130 140 150
SVVNPASYHN VQEEWVPELK DCMPHVPYVL IGTQIDLRDD PKTLARLLYM
160 170 180 190 200
KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ KGLKAVFDEA ILTIFHPKKK
210
KKRCSEGHSC CSII
Length:214
Mass (Da):23,821
Last modified:March 1, 2001 - v1
Checksum:iAAD2E2CED036F48C
GO
Isoform 2 (identifier: Q9H4E5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-134: Missing.

Note: No experimental confirmation available. Could be created by usage of an unusual splicing donor site.
Show »
Length:192
Mass (Da):21,255
Checksum:iF20844F6EC738489
GO

Sequence cautioni

The sequence BAB55013 differs from that shown. Reason: Frameshift at position 143.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3C → R in BAB55013 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007231113 – 134Missing in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276567 mRNA. Translation: CAC06611.1.
AF309563 mRNA. Translation: AAL09440.1.
AK027278 mRNA. Translation: BAB55013.1. Frameshift.
AK027351 mRNA. Translation: BAB55055.1.
AF498977 mRNA. Translation: AAM21124.1.
BC062575 mRNA. Translation: AAH62575.1.
CCDSiCCDS9757.1. [Q9H4E5-1]
RefSeqiNP_065714.1. NM_020663.4. [Q9H4E5-1]
UniGeneiHs.656339.

Genome annotation databases

EnsembliENST00000316754; ENSP00000316729; ENSG00000126785. [Q9H4E5-1]
GeneIDi57381.
KEGGihsa:57381.
UCSCiuc001xgb.3. human. [Q9H4E5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRHOJ_HUMAN
AccessioniPrimary (citable) accession number: Q9H4E5
Secondary accession number(s): Q96KC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: March 1, 2001
Last modified: August 30, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families