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Protein

Nuclear RNA export factor 3

Gene

NXF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a tissue-specific nuclear mRNA export factor.

GO - Molecular functioni

GO - Biological processi

  • mRNA export from nucleus Source: UniProtKB
  • poly(A)+ mRNA export from nucleus Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear RNA export factor 3
Alternative name(s):
TAP-like protein 3
Short name:
TAPL-3
Gene namesi
Name:NXF3
Synonyms:TAPL3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:8073. NXF3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nuclear RNA export factor complex Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi300L → R: Inactivates CRM1 binding; when associated with R-302. 1 Publication1
Mutagenesisi302L → R: Inactivates CRM1 binding; when associated with R-300. 1 Publication1

Organism-specific databases

DisGeNETi56000.
OpenTargetsiENSG00000147206.
PharmGKBiPA31860.

Polymorphism and mutation databases

BioMutaiNXF3.
DMDMi20455187.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002205341 – 531Nuclear RNA export factor 3Add BLAST531

Proteomic databases

EPDiQ9H4D5.
PaxDbiQ9H4D5.
PeptideAtlasiQ9H4D5.
PRIDEiQ9H4D5.

PTM databases

iPTMnetiQ9H4D5.
PhosphoSitePlusiQ9H4D5.

Expressioni

Tissue specificityi

Expressed at high level in testis and at low level in a small number of tissues.

Gene expression databases

BgeeiENSG00000147206.
CleanExiHS_NXF3.
ExpressionAtlasiQ9H4D5. baseline and differential.
GenevisibleiQ9H4D5. HS.

Organism-specific databases

HPAiHPA003132.
HPA046757.

Interactioni

Subunit structurei

Interacts with NXT1, NXT2, E1B-AP5 and CRM1 nuclear export factor.

Binary interactionsi

WithEntry#Exp.IntActNotes
NXT1Q9UKK68EBI-750038,EBI-301889
NXT2Q9NPJ87EBI-750038,EBI-752122
NXT2Q9NPJ8-34EBI-750038,EBI-10698339
SKILP127573EBI-750038,EBI-2902468

Protein-protein interaction databases

BioGridi121025. 6 interactors.
IntActiQ9H4D5. 3 interactors.
STRINGi9606.ENSP00000378504.

Structurei

3D structure databases

ProteinModelPortaliQ9H4D5.
SMRiQ9H4D5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini113 – 192RRMAdd BLAST80
Domaini344 – 494NTF2PROSITE-ProRule annotationAdd BLAST151

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi296 – 302Leu-rich7
Compositional biasi481 – 484Poly-Ser4

Domaini

Lacks C-terminal domain that mediates direct interactions with nucleoporins.
Contains a novel CRM1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain.
The RNA-binding domain is a non-canonical RNP-type domain.

Sequence similaritiesi

Belongs to the NXF family.Curated
Contains 1 NTF2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3763. Eukaryota.
ENOG410XR55. LUCA.
GeneTreeiENSGT00390000007539.
HOGENOMiHOG000236269.
HOVERGENiHBG013199.
InParanoidiQ9H4D5.
KOiK14284.
OMAiDQTHVNM.
OrthoDBiEOG091G04QA.
PhylomeDBiQ9H4D5.
TreeFamiTF314566.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR012677. Nucleotide-bd_a/b_plait.
IPR030216. NXF3.
IPR030217. NXF_fam.
IPR015245. Tap_RNA-bd.
[Graphical view]
PANTHERiPTHR10662. PTHR10662. 1 hit.
PTHR10662:SF12. PTHR10662:SF12. 1 hit.
PfamiPF02136. NTF2. 1 hit.
PF09162. Tap-RNA_bind. 1 hit.
[Graphical view]
ProDomiPD043466. Tap_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54427. SSF54427. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H4D5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLPSGHTTG HTDQVVQRRA RCWDIYQRRF SSRSEPVNPG MHSSSHQQQD
60 70 80 90 100
GDAAMHGAHM DSPVRYTPYT ISPYNRKGSF RKQDQTHVNM EREQKPPERR
110 120 130 140 150
MEGNMPDGTL GSWFKITVPF GIKYNEKWLL NLIQNECSVP FVPVEFHYEN
160 170 180 190 200
MHASFFVENA SIAYALKNVS GKIWDEDNEK ISIFVNPAGI PHFVHRELKS
210 220 230 240 250
EKVEQIKLAM NQQCDVSQEA LDIQRLPFYP DMVNRDTKMA SNPRKCMAAS
260 270 280 290 300
LDVHEENIPT VMSAGEMDKW KGIEPGEKCA DRSPVCTTFS DTSSNINSIL
310 320 330 340 350
ELFPKLLCLD GQQSPRATLC GTEAHKRLPT CKGSFFGSEM LKNLVLQFLQ
360 370 380 390 400
QYYLIYDSGD RQGLLSAYHD EACFSLSIPF NPEDSAPSSF CKFFKDSRNI
410 420 430 440 450
KILKDPYLRG ELLKHTKLDI VDSLSALPKT QHDLSSFLVD MWYQTEWMLC
460 470 480 490 500
FSVNGVFKEV EGQSQGSVLA FTRTFIATPG SSSSLCIVND KLFVRDTSHQ
510 520 530
GTQSALFTLV PTAFSSSVPA FSQEQQKMLP S
Length:531
Mass (Da):60,102
Last modified:March 1, 2001 - v1
Checksum:i3A47C7D35FC396B9
GO
Isoform 2 (identifier: Q9H4D5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: Missing.
     297-322: NSILELFPKLLCLDGQQSPRATLCGT → KSVVPSVTTWDPDLCLIAPFCRRRQH
     323-531: Missing.

Note: No experimental confirmation available.
Show »
Length:233
Mass (Da):26,653
Checksum:i98A4095DE20120F0
GO

Sequence cautioni

The sequence CAC20434 differs from that shown. Reason: Frameshift at position 463.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050419186N → I.Corresponds to variant rs2301387dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0570681 – 89Missing in isoform 2. 1 PublicationAdd BLAST89
Alternative sequenceiVSP_057069297 – 322NSILE…TLCGT → KSVVPSVTTWDPDLCLIAPF CRRRQH in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_057070323 – 531Missing in isoform 2. 1 PublicationAdd BLAST209

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277527 mRNA. Translation: CAC16589.1.
AF346619 mRNA. Translation: AAL07564.1.
AK302586 mRNA. Translation: BAG63839.1.
Z75746 Genomic DNA. Translation: CAI41984.1.
CH471190 Genomic DNA. Translation: EAW54722.1.
BC031616 mRNA. Translation: AAH31616.1.
AJ277660 mRNA. Translation: CAC20434.1. Frameshift.
CCDSiCCDS14503.1. [Q9H4D5-1]
RefSeqiNP_071335.1. NM_022052.1. [Q9H4D5-1]
UniGeneiHs.60386.

Genome annotation databases

EnsembliENST00000395065; ENSP00000378504; ENSG00000147206. [Q9H4D5-1]
GeneIDi56000.
KEGGihsa:56000.
UCSCiuc004eju.5. human. [Q9H4D5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277527 mRNA. Translation: CAC16589.1.
AF346619 mRNA. Translation: AAL07564.1.
AK302586 mRNA. Translation: BAG63839.1.
Z75746 Genomic DNA. Translation: CAI41984.1.
CH471190 Genomic DNA. Translation: EAW54722.1.
BC031616 mRNA. Translation: AAH31616.1.
AJ277660 mRNA. Translation: CAC20434.1. Frameshift.
CCDSiCCDS14503.1. [Q9H4D5-1]
RefSeqiNP_071335.1. NM_022052.1. [Q9H4D5-1]
UniGeneiHs.60386.

3D structure databases

ProteinModelPortaliQ9H4D5.
SMRiQ9H4D5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121025. 6 interactors.
IntActiQ9H4D5. 3 interactors.
STRINGi9606.ENSP00000378504.

PTM databases

iPTMnetiQ9H4D5.
PhosphoSitePlusiQ9H4D5.

Polymorphism and mutation databases

BioMutaiNXF3.
DMDMi20455187.

Proteomic databases

EPDiQ9H4D5.
PaxDbiQ9H4D5.
PeptideAtlasiQ9H4D5.
PRIDEiQ9H4D5.

Protocols and materials databases

DNASUi56000.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000395065; ENSP00000378504; ENSG00000147206. [Q9H4D5-1]
GeneIDi56000.
KEGGihsa:56000.
UCSCiuc004eju.5. human. [Q9H4D5-1]

Organism-specific databases

CTDi56000.
DisGeNETi56000.
GeneCardsiNXF3.
HGNCiHGNC:8073. NXF3.
HPAiHPA003132.
HPA046757.
MIMi300316. gene.
neXtProtiNX_Q9H4D5.
OpenTargetsiENSG00000147206.
PharmGKBiPA31860.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3763. Eukaryota.
ENOG410XR55. LUCA.
GeneTreeiENSGT00390000007539.
HOGENOMiHOG000236269.
HOVERGENiHBG013199.
InParanoidiQ9H4D5.
KOiK14284.
OMAiDQTHVNM.
OrthoDBiEOG091G04QA.
PhylomeDBiQ9H4D5.
TreeFamiTF314566.

Miscellaneous databases

ChiTaRSiNXF3. human.
GenomeRNAii56000.
PROiQ9H4D5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000147206.
CleanExiHS_NXF3.
ExpressionAtlasiQ9H4D5. baseline and differential.
GenevisibleiQ9H4D5. HS.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR012677. Nucleotide-bd_a/b_plait.
IPR030216. NXF3.
IPR030217. NXF_fam.
IPR015245. Tap_RNA-bd.
[Graphical view]
PANTHERiPTHR10662. PTHR10662. 1 hit.
PTHR10662:SF12. PTHR10662:SF12. 1 hit.
PfamiPF02136. NTF2. 1 hit.
PF09162. Tap-RNA_bind. 1 hit.
[Graphical view]
ProDomiPD043466. Tap_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54427. SSF54427. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNXF3_HUMAN
AccessioniPrimary (citable) accession number: Q9H4D5
Secondary accession number(s): B4DYS7, Q5H9I1, Q9H1A9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.